Cell Death and Differentiation

FIGURE 2

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ADAM10 regulates FasL cell surface expression and modulates FasL-induced cytotoxicity and activation-induced cell death

M Schulte, K Reiss, M Lettau, T Maretzky, A Ludwig, D Hartmann, B de Strooper, O Janssen and P Saftig

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Figure 2.

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Recombinant human FasL is cleaved by recombinant human ADAM10 in vitro. Recombinant FasL was incubated with recombinant ADAM10 or ADAM17 in reaction buffer. Before starting the reaction, a control sample was taken, representing the intact FasL. After 5 h and after 14 h, samples were immediately mixed with laemmli buffer and analyzed on SDS-PAGE. ADAM10 and ADAM17 were detected by immunoblot with anti-His-antibodies. For FasL detection anti-Flag-antibodies against the N-terminal Flag tag were used. The blot is representative of three independent experiments. NTF: NTFs of FasL; FL: full-length FasL

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