Cell Death and Differentiation

FIGURE 5

FROM:

Lack of involvement of mitochondrial factors in caspase activation in a Drosophila cell-free system

J C Means, I Muro and R J Clem

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Figure 5.

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Lysed S2 cell mitochondria are capable of inducing caspase activation in S100 obtained from human cells but not in S100 from Drosophila cells. S100 lysate obtained from untreated 2 times 109 Drosophila S2 (a) or 5 times 106 human 293 (b) cells was incubated with either S2 or 293 P10, purified cytochrome c, or S100 from 1 times 108 UV-treated S2 cells treated with the caspase inhibitor zVAD-fmk and caspase activity was determined using DEVD-afc as substrate

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