FIGURE 1
FROM:
Lack of involvement of mitochondrial factors in caspase activation in a Drosophila cell-free system
J C Means, I Muro and R J Clem
BACK TO ARTICLEFigure 1.
Cytochrome c is not released from S2 cell mitochondria during UV-induced apoptosis. (a) Drosophila S2 cells were UV irradiated and at the indicated times the P10 and S100 fractions were isolated and analyzed by immunoblotting with anti-cytochrome c and anti-DrICE antibodies (above). Caspase activity was measured in S100 from UV-treated cells using DEVD-afc as substrate (below). (b) Human 293 cells were UV irradiated and at the indicated times the P10 and S100 fractions were isolated and analyzed by immunoblotting with anti-cytochrome c antibody (above). Caspase activity was measured in S100 from UV-treated cells using DEVD-afc as substrate (below)
