Cell Death and Differentiation - Figure 1 for article: White adipocytes are less prone to apoptotic stimuli than brown adipocytes in rodent

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White adipocytes are less prone to apoptotic stimuli than brown adipocytes in rodent

E Nisoli, A Cardile, A Bulbarelli, L Tedesco, R Bracale, V Cozzi, M Morroni, S Cinti, A Valerio and M O Carruba

Figure 1.

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Different grades of apoptosis in brown and white adipocytes in culture. (a) Phase-contrast photomicrographs of brown and white adipocytes differentiated in culture. Magnification, |[times]| 20. (b) DNA fragmentation in cells treated for 5|[thinsp]|h with either 1 or 10|[thinsp]|nM TNF-|[alpha]| plus CHX. M, molecular marker; C, untreated cells. (c) Cytofluorimetric analysis. The cells were treated with 10|[thinsp]|nM TNF-|[alpha]| plus CHX for 5|[thinsp]|h and analyzed for AnnexinV-FITC positivity: square 2, propidium iodide-positive cells; square 3, nonapoptotic cells; square 4, apoptotic cells. (d, e) Cytofluorometer analysis of time- and dose-dependence. Open bars, white adipocytes; closed bars, brown adipocytes. The bars represent the mean values|[plusmn]|S.E.M. of three separate experiments. ^|[ast]| P<0.01: difference from untreated cells (C) at time 0

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FIGURE 1

White adipocytes are less prone to apoptotic stimuli than brown adipocytes in rodent

Figure 1.