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Post-Transplant Events

Comparative analysis of a double primer PCR assay with plasma, leukocytes and antigenemia for diagnosis of active human cytomegalovirus infection in bone marrow transplant patients

Bone Marrow Transplantation volume 35, pages 595–599 (2005)Cite this article

Summary:

The aim of the study was to determine the prognostic value of a double primer PCR assay to detect human cytomegalovirus (HCMV) infection or disease in bone marrow transplant (BMT) recipients. A total of 209 blood samples including peripheral blood mononuclear cells (PBMN), polymorphonuclear (PMN) leukocytes and plasma from 26 BMT recipients were tested by PCR assay. To discriminate between latent and active HCMV infection, 177 blood samples were also tested by a quantitative antigenemia assay. HCMV serology status of donors and recipients was determined before transplantation by an enzyme immunosorbent assay method. Using the double primer PCR assay, the number of positive samples increased by an average of 11.6%. Symptomatic active HCMV infection was diagnosed in 14 (53.8%) out of 26 BMT patients. There was a good association between double primer PCR assay of PMN leukocytes and antigenemia assays for detection of active HCMV infection in all patients. Detection of HCMV DNA in PMN leukocytes of BMT patients by double primer PCR assay can be an alternative method for antigenemia assay. However, quantitative PCR methods will be necessary for monitoring antiviral treatment.

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References

  1. Ochs L, Ou Shu X, Miller J et al. Late infections after allogeneic bone marrow transplant: comparison of incidence in related and unrelated donor transplant recipients. Blood 1995; 86: 3979–3986.

    CAS  Google Scholar 

  2. Maltezou HC, Kafetzis DA, Abisaid D et al. Viral infections in children undergoing hematopoietic stem cell transplant. J Pediatr Infect Dis 2000; 19: 307–312.

    Article  CAS  Google Scholar 

  3. Pass RF . Cytomegalovirus. In: Knipp DM, Howley PM (eds). Fields Virology, 4th edn. Lippincott-Williams & Wilkins, 2001, pp 2675–2706.

    Google Scholar 

  4. Van Der Bij W, Schirm J, Torensma R et al. Comparison between viremia and antigenemia for detection of cytomegalovirus in blood. J Clin Micorbiol 1998; 26: 2531–2535.

    Google Scholar 

  5. Cope AV, Sweny P, Sabin C et al. Quantity of cytomegalovirus viruria is a major risk factor for cytomegalovirus disease after renal transplantation. J Med Virol 1997; 52: 200–205.

    Article  CAS  Google Scholar 

  6. Van Den Berg AP, Klompmaker IJ, Haagsma EB et al. Antigenemia in the diagnosis and monitoring of active cytomegalovirus infection after liver transplantation. J Infect Dis 1991; 164: 265–270.

    Article  CAS  Google Scholar 

  7. Boeckh M, Hawkins G, Myerson D et al. Plasma PCR for cytomegalovirus DNA after allogeneic marrow transplantation: comparison with PCR using peripheral blood leukocytes, pp65 antigenemia, and viral culture. Transplantation 1997; 64: 108–113.

    Article  CAS  Google Scholar 

  8. Visser CE, Van zeijl CJG, De klerk EPA et al. First experiences with an accelerated CMV antigenemia test: CMV Brite Turbo assay. J Clin Virol 2000; 17: 65–68.

    Article  CAS  Google Scholar 

  9. Marianne L-V, Marie O, Richard D et al. Monitoring cytomegalovirus infection in adult and pediatric bone marrow transplant recipients by a real-time PCR assay performed with blood plasma marianne. J Clin Microbiol 2003; 41: 2040–2046.

    Article  Google Scholar 

  10. Erice A, Holm MA, Gill PC et al. Cytomegalovirus (CMV) antigenemia assay is more sensitive than shell vial cultures for rapid detection of CMV in polymorphonuclear blood leukocytes. J Clin Microbiol 1992; 30: 2822–2825.

    CAS  PubMed  PubMed Central  Google Scholar 

  11. Van der Bij W, Torensma R, Van son WJ et al. Rapid immunodiagnosis of active cytomegalovirus infection by monoclonal antibody staining of blood leucocytes. J Med Virol 1988; 25: 179–188.

    Article  CAS  Google Scholar 

  12. Caballero OL, Menezes CL, Costa MC et al. Highly sensitive single-step PCR protocol for diagnosis and monitoring of human cytomegalovirus infection in renal transplant recipients. J Clin Microbiol 1997; 35: 3192–3197.

    CAS  PubMed  PubMed Central  Google Scholar 

  13. Ljungman P, Griffiths P, Paya C . Definitions of cytomegalovirus infection and disease in transplant recipients. Clin Infect Dis 2002; 34: 1094–1097.

    Article  Google Scholar 

  14. Stephen KN, Ho Chi-Yuen Lo, Ignatius KPC et al. Rapid cytomegalovirus pp65 antigenemia assay by direct erythrocyte lysis and immunofluorescence staining. J Clin Microbiol 1998; 36: 638–640.

    Google Scholar 

  15. Sambrook JK, Russell DW, Irwin N et al. Molecular Cloning. A Laboratory Manual, Vol. 3, 3rd edn. Cold Spring Harbor Laboratory Press: New York, 2001, p A1.23.

    Google Scholar 

  16. Saiki RK, Gelfand DH, Stoffel S et al. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 1988; 239: 487–491.

    Article  CAS  Google Scholar 

  17. Yakushiji K, Gondo H, Kamezaki K et al. Monitoring of cytomegalovirus reactivation after allogeneic stem cell transplantation: comparison of an antigenemia assay and quantitative real-time polymerase chain reaction. Bone Marrow Transplant 2002; 29: 599–606.

    Article  CAS  Google Scholar 

  18. Yun Z, Lewensohn FI, Ljungman P et al. Real time monitoring of cytomegalovirus infections after stem cell transplantation using TaqMan polymerase chain reaction assays. Transplantation 2000; 27: 1733–1736.

    Article  Google Scholar 

  19. Abecassis MM, Koffron AJ, Kaplan B et al. The role of PCR in the diagnosis and management of CMV in solid organ recipients. Transplantation 1997; 63: 275–279.

    Article  CAS  Google Scholar 

  20. Tanabe K, Tokumoto T, Ishikawa N et al. Comparative study of cytomegalovirus (CMV) antigenemia assay, polymerase chain reaction, serology, and shell vial assay in the early diagnosis and monitoring of CMV infection after renal transplantation. Transplantation 1997; 27: 1721–1725.

    Article  Google Scholar 

  21. Roberts TC, Brennan DC, Buller RS et al. Quantitative polymerase chain reaction to predict occurrence of symptomatic cytomegalovirus infection and assess response to ganciclovir therapy in renal transplant recipients. J Infect Dis 1998; 178: 626–635.

    Article  CAS  Google Scholar 

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Acknowledgements

This project was financially supported by Tarbiat Moddares University, Tehran Iran and Shiraz University of Medical Sciences, Shiraz, Iran.

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Authors and Affiliations

  1. Virology Section, School of Medicine, Tarbiat Modarres University, Tehran, Iran

    R Yaghobi, F Sabahi & M H Roustaee

  2. Organ Transplant Research Center, Clinical Virology Section, Namazi Hospital, Shiraz University of Medical Sciences, Shiraz, Iran

    A Behzad-Behbahani

  3. Professor Alborzi Clinical Microbiology Research Center, Namazi Hospital, Shiraz University of Medical Sciences, Shiraz, Iran

    A Alborzi

  4. Hematology Research Center, Hematology, Oncology and Bone Marrow Transplant Center, Shiraz University of Medical Sciences, Shiraz, Iran

    M Ramzi & H Nourani

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  1. R Yaghobi
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Correspondence to A Behzad-Behbahani.

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Yaghobi, R., Behzad-Behbahani, A., Sabahi, F. et al. Comparative analysis of a double primer PCR assay with plasma, leukocytes and antigenemia for diagnosis of active human cytomegalovirus infection in bone marrow transplant patients. Bone Marrow Transplant 35, 595–599 (2005). https://doi.org/10.1038/sj.bmt.1704797

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  • DOI: https://doi.org/10.1038/sj.bmt.1704797

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