¹Department of Haematology, Christian Medical College, Vellore, Tamil Nadu, India

Correspondence: Dr B George, Department of Haematology, Christian Medical College, Vellore 632004, Tamil Nadu, India. E-mail: biju@cmcvellore.ac.in

Received 23 April 2004; Accepted 16 September 2004; Published online 10 January 2005.

Abstract

A fludarabine-based protocol (fludarabine (25 mg/m²/day 6 days), cyclophosphamide (10 mg/kg/day 2 days) and ATG (ATGAM 10 mg/kg/day 4 days)) was used in four multiply transfused Fanconi's anemia (FA) patients aged 5–15 years to reduce rejection during allogeneic bone marrow transplantation (BMT). Graft-versus-host disease (GVHD) prophylaxis consisted of cyclosporine and mini methotrexate. The graft source was G-CSF-stimulated bone marrow or peripheral blood stem cells (PBSC) in two patients each. All patients engrafted with median time to ANC>500/mm³ being 14 days (range: 12–17) and unsupported platelet count >20 ,000/mm³ being 13 days (range: 11–18). One patient had secondary graft rejection on day 56 and expired on day 69 due to fungal pneumonia. One patient who developed acute myeloid leukemia on day 56 underwent successful induction with cytosine and daunorubicin followed by peripheral blood stem cell (PBSC) rescue on day 70 and is presently in remission with complete donor chimerism and grade I GVHD. At a median follow-up of 13 months (range: 4–21), three patients (75%) are well with complete donor chimerism. Addition of fludarabine to the conditioning regimen for BMT in FA can provide additional immunosuppression for engraftment without increasing toxicity.