Summary:
Recombinant human granulocyte colony-stimulating factor (rhG-CSF) is frequently used to mobilize CD34+ cells in healthy donors and patient with malignant diseases prior to peripheral blood stem cell (PBSC) harvest. To analyze the effects of rhG-CSF on morphology and genotype of white blood cells, a novel multiparametric cell scanning system that combines morphologic, immune and genotypic analyses of the same cells was used. We report here that tetraploid myeloid cells are present in the peripheral blood of donors treated with rhG-CSF. The tetraploidy was detected in up to 0.6% of differentiated myeloid cells and all observed CD34+ cells were diploid. Thus, short treatment with rhG-CSF of PBSC donors induces numerfical chromosomal alterations in a small subset of mature myeloid cells.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Welte K, Gabrilove J, Bronchud MH et al. Filgrastim (r-metHuG-CSF): the first 10 years. Blood 1996; 88: 1907–1929.
Link DC . Mechanisms of granulocyte colony-stimulating factor-induced hematopoietic progenitor-cell mobilization. Semin Hematol 2000; 37: 25–32.
Cleaver SA, Goldman JM . Use of G-CSF to mobilise PBSC in normal healthy donors–an international survey. Bone Marrow Transplant 1998; 21 (Suppl. 3): S29–S31.
Price TH . The use of G-CSF in normal neutrophil donors. Ann Med Interne (Paris) 2000; 151: 115–122.
Volk EE, Domen RE, Smith ML . An examination of ethical issues raised in the pretreatment of normal volunteer granulocyte donors with granulocyte colony-stimulating factor. Arch Pathol Lab Med 1999; 123: 508–513.
Anderlini P, Donato M, Chan KW et al. Allogeneic blood progenitor cell collection in normal donors after mobilization with filgrastim: the M.D. Anderson Cancer Center experience. Transfusion 1999; 39: 555–560.
Anderlini P, Korbling M, Dale D et al. Allogeneic blood stem cell transplantation: considerations for donors. Blood 1997; 90: 903–908.
Tidow N, Kasper B, Welte K . Clinical implications of G-CSF receptor mutations. Crit Rev Oncol Hematol 1998; 28: 1–6.
Stroncek D, Anderlini P . Mobilized PBPC concentrates: a maturing blood component. Transfusion 2001; 41: 168–171.
Stroncek DF, Yau YY, Oblitas J, Leitman SF . Administration of G-CSF plus dexamethasone produces greater granulocyte concentrate yields while causing no more donor toxicity than G-CSF alone. Transfusion 2001; 41: 1037–1044.
Platzbecker U, Prange-Krex G, Bornhauser M et al. Spleen enlargement in healthy donors during G-CSF mobilization of PBPCs. Transfusion 2001; 41: 184–189.
Campbell LJ, Maher DW, Tay DL et al. Marrow proliferation and the appearance of giant neutrophils in response to recombinant human granulocyte colony stimulating factor (rhG-CSF). Br J Haematol 1992; 80: 298–304.
Ulich TR, del Castillo J, Souza L . Kinetics and mechanisms of recombinant human granulocyte-colony stimulating factor-induced neutrophilia. Am J Pathol 1988; 133: 630–638.
Shimoni A, Nagler A, Kaplinsky C et al. Chimerism testing and detection of minimal residual disease after allogeneic hematopoietic transplantation using the bioView (Duet) combined morphological and cytogenetical analysis. Leukemia 2002; 16: 1413–1418; discussion 1419–1422.
Bielorai B, Golan H, Trakhtenbrot L et al. Combined analysis of morphology and FISH in follow-up of minimal residual disease (MRD) in a child with ph+ acute lymphoblastic leukemia. Cancer Genet Cytogenet 2002; 138: 64–68.
Jakubowski A, Gabrilove J . Granulocyte colony stimulating factor (G-CSF): biology and clinical status. Cancer Biother Radiopharm 1996; 11: 5–20.
Clarke DJ, Gimenez-Abian JF . Checkpoints controlling mitosis. Bioessays 2000; 22: 351–363.
Schmidt-Mende J, Tehranchi R, Forsblom AM et al. Granulocyte colony-stimulating factor inhibits Fas-triggered apoptosis in bone marrow cells isolated from patients with refractory anemia with ringed sideroblasts. Leukemia 2001; 15: 742–751.
Villunger A, O'Reilly LA, Holler N et al. Fas ligand, Bcl-2, granulocyte colony-stimulating factor, and p38 mitogen-activated protein kinase: regulators of distinct cell death and survival pathways in granulocytes. J Exp Med 2000; 192: 647–658.
Kaplinsky C, Lotem J, Sachs L . Protection of human myeloid leukemic cells against doxorubicin-induced apoptosis by granulocyte–macrophage colony-stimulating factor and interleukin 3. Leukemia 1996; 10: 460–465.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kaplinsky, C., Trakhtenbrot, L., Hardan, I. et al. Tetraploid myeloid cells in donors of peripheral blood stem cells treated with rhG-CSF. Bone Marrow Transplant 32, 31–34 (2003). https://doi.org/10.1038/sj.bmt.1703902
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/sj.bmt.1703902
Keywords
This article is cited by
-
Long-term follow-up of cancer and catastrophic diseases in hematopoietic stem cell donors: a comprehensive matched cohort study
Bone Marrow Transplantation (2024)
-
A review of the genetic and long-term effects of G-CSF injections in healthy donors: a reassuring lack of evidence for the development of haematological malignancies
Bone Marrow Transplantation (2015)
-
Long-Term Inhaled Granulocyte Macrophage–Colony-Stimulating Factor in Autoimmune Pulmonary Alveolar Proteinosis: Effectiveness, Safety, and Lowest Effective Dose
Clinical Drug Investigation (2014)
-
Allogeneic hematopoietic stem cell donation—standardized assessment of donor outcome data: A consensus statement from the Worldwide Network for Blood and Marrow Transplantation (WBMT)
Bone Marrow Transplantation (2013)
-
Haematological malignancies in sibling and unrelated donors of allogeneic peripheral stem cells mobilised with G-CSF filgrastim: a transplant centre and Czech National Marrow Donors Registry experience
Bone Marrow Transplantation (2012)