Haemopoietic Growth Factor
Bone Marrow Transplantation (2003) 32, 31–34. doi:10.1038/sj.bmt.1703902
Tetraploid myeloid cells in donors of peripheral blood stem cells treated with rhG-CSF
C Kaplinsky1, L Trakhtenbrot1, I Hardan2, M Reichart3, M Daniely3, A Toren1, N Amariglio1, G Rechavi1 and S Izraeli1
- 1Department of Pediatric Hemato-Oncology, The Chaim Sheba Medical Center, Tel-Hashomer, Israel
- 2Institute of Hematology, The Chaim Sheba Medical Center, Tel-Hashomer, Israel and Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel
- 3BioView Ltd, Nes Ziona, Israel
Correspondence: Dr S Izraeli, Department of Pediatric Hemato-Oncology, Sheba Medical Center, Tel-Hashomer 52621, Israel
Received 5 September 2002; Accepted 5 November 2002.
Abstract
Recombinant human granulocyte colony-stimulating factor (rhG-CSF) is frequently used to mobilize CD34+ cells in healthy donors and patient with malignant diseases prior to peripheral blood stem cell (PBSC) harvest. To analyze the effects of rhG-CSF on morphology and genotype of white blood cells, a novel multiparametric cell scanning system that combines morphologic, immune and genotypic analyses of the same cells was used. We report here that tetraploid myeloid cells are present in the peripheral blood of donors treated with rhG-CSF. The tetraploidy was detected in up to 0.6% of differentiated myeloid cells and all observed CD34+ cells were diploid. Thus, short treatment with rhG-CSF of PBSC donors induces numerfical chromosomal alterations in a small subset of mature myeloid cells.
Keywords:
G-CSF, cytogenetics, tetraploidy, peripheral stem cell harvest, peripheral stem cell transplantation
