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| February 2001, Volume 27, Number 4, Pages 425-431 |
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| Immune Recovery |
| Reconstitution of the CD45RO+ and CD20+ lymphoid marrow population following allogeneic bone marrow transplantation for Ph+ CML |
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| J Thiele1, H M Kvasnicka1, D W Beelen2, A Welter1, S Schneider1, L D Leder3 and U W Schaefer2 |
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1Institute of Pathology, University of Cologne, Germany
2Department of Bone Marrow Transplantation, University of Essen, Essen, Germany
3Institutes of Pathology, Universities of Cologne and Essen, Essen, Germany
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Correspondence to: Dr J Thiele, Institute of Pathology, University of Cologne, Joseph-Stelzmannstr.9, D-50924 Cologne, Germany
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| Abstract |
| Following bone marrow transplantation (BMT) investigations on the recovery of the B and T lymphocyte populations have focused on the peripheral blood and only marginally regard the bone marrow. An immunohistochemical and morphometric study was performed on 352 trephine biopsies derived from 123 patients with chronic myelogenous leukemia (CML) at standardized endpoints before and after allogeneic BMT and compared to a control group. The purpose of this investigation was to quantify the B-CD20+ and T-CD45RO+ lymphocyte subsets and to determine possible relationships with the occurrence of acute and chronic GVHD. Moreover, we studied the dynamics of lymphocyte repopulation in the post-transplant period, correlations with the total peripheral lymphocyte count and differences associated with sibling vs alternate HLA-compatible (unmanipulated) marrow grafts. Morphometric analysis revealed a very fast regeneration of CD45RO+ and CD20+ marrow lymphocytes in the first 2 weeks following BMT. In less than 2 months, in most patients, the post-transplant quantity of lymphocytes was comparable to that of the normal bone marrow. This finding was opposed to the profound depression of the absolute lymphocyte count in the peripheral blood. No relevant relationships could be calculated between engraftment status and the lymphocyte repopulation in the bone marrow. On the other hand, significant correlations were calculable between the development of (chronic and acute) GVHD including severity with the number of CD45RO+ lymphocytes. In non-related graft constellations a more frequent evolution of acute grade III + IV GVHD was detectable. This complication was accompanied by an increased quantity of CD45RO+ lymphocytes in the marrow. Bone Marrow Transplantation (2001) 27, 425-431. |
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| Keywords |
| lymphocyte subsets (CD45RO+, CD20+); bone marrow transplantation; CML; GVHD; trephine biopsies; immunohistochemistry |
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| Received 3 July 2000; accepted 15 November 2000 |
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| February 2001, Volume 27, Number 4, Pages 425-431 |
| Table of contents Previous Abstract Next Full text PDF |
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