British Journal of Cancer

TABLE 1

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Assessing proliferation, cell-cycle arrest and apoptotic end points in human buccal punch biopsies for use as pharmacodynamic biomarkers in drug development

D R Camidge, M N Pemberton, J W Growcott, D Johnstone, P J Laud, J R Foster, K J Randall and A M Hughes

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Table 1. Details of antibodies/techniques used

Figure and tables index
Target Supplier Host/type Catalogue number Dilution Detection System
Total-pRbCSTMouse monoclonal93091 : 100Mouse Envision+
Phospho(S249/T252)-pRbBiosourceRabbit polyclonal44-5841 : 1000Rabbit Envision+
Total-p27RDIRabbit polyclonalP27CabrX1 : 100Rabbit Envision+
Phospho(T187)-p27UpstateRabbit polyclonal06-9961 : 100Rabbit Envision+
p53NovoCastraRabbit polyclonalNCL-p53-CM11 : 2500Rabbit Envision+
Cyclin ANeomarkersMouse monoclonalMS-1061S11 : 40Mouse Envision+
Cyclin ENovoCastraMouse monoclonalNCL-CYCLIN E1 : 20Mouse Envision+
Ki67DakoMouse monoclonalM72401 : 25Mouse Envision+
Phospho(S10)-Histone H3UpstateRabbit polyclonal06–5701 : 1000Rabbit Envision+
p21BD PharmingenMouse monoclonal6102341 : 25Mouse Envision+
Cleaved-Caspase-3CSTRabbit polyclonal96611 : 300Rabbit Envision+
M30RocheMouse monoclonal2 140 3491 : 200Mouse Envision+
TUNEL (in situ cell death kit)RocheN/A1 684 817  

 pRb=phosphorylated pRb; phospho-p27=phosphorylated p27; TUNEL=terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labelling.

 CST–New England Biolabs (UK) Ltd, Hitchin, Hertfordshire, UK; Biosource–B-1400 Nivelles, Belgium; RDI–Concord MA 01742-3049 USA; Upstate–Dundee, UK; Novocastra–Newcastle upon Tyne, UK; Neomarkers–Lab Vision (UK) Ltd, Newmarket, Suffolk, UK; Dako–Ely, Cambridgeshire, UK; BD Pharmingen–Cowley, Oxford, UK.

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