TABLE 1
FROM:
In vitro hypoxia-conditioned colon cancer cell lines derived from HCT116 and HT29 exhibit altered apoptosis susceptibility and a more angiogenic profile in vivo
K Yao, J A Gietema, S Shida, M Selvakumaran, X Fonrose, N B Haas, J Testa and P J O'Dwyer
BACK TO ARTICLETable 1. Growth characteristics of HT29 and HCT116-derived cell lines before and after repeated 4 h exposures to hypoxia. Doubling times and cell cycle distribution in oxic conditions were measured under standard culture conditions
| Cell line | HT29 | HP40 | HCT 116 | HCP40 |
|---|---|---|---|---|
| Doubling time (h) | 25.9 2.3 | 33.81.9*
| 28.5 1.7 | 20.51.1*
|
| Cell cycle distribution | ||||
| Oxic conditions | ||||
| G0/G1 (%) | 72.5 | 71.5 | 50.6 | 54.8 |
| G2/M (%) | 8.5 | 9.5 | 23.5 | 20.2 |
| S (%) | 13.9 | 16.5 | 22.7 | 20.5 |
| Hypoxic conditions | ||||
| G0/G1 (%) | 59.3 | 74.1 | 62.4 | 47.5 |
| G2/M (%) | 10.7 | 11.5 | 10.2 | 9.4 |
| S (%) | 18.2 | 12.3 | 11.9 | 9.2 |
Cell cycle analysis under hypoxic conditions was conducted after a 24 h exposure to hypoxia, and included adherent and floating cells.
* denote a statistically significant difference between parental cell line and its hypoxia-conditioned derivative with P-value <0.05).
