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Early neoplastic and metastatic mammary tumours of transgenic mice detected by 5-aminolevulinic acid-stimulated protoporphyrin IX accumulation
A M Dorward, K S Fancher, T M Duffy, W G Beamer and H Walt
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Figure 1.
Digital image capture of PpIX-stimulated red fluorescence in tumours of three mammary tumour-bearing transgenic strains 1 h following in vivo administration of 200 mg kg-1 ALA, with corresponding H&E stained histological sections. (A, B) Red fluorescence in a papillary adenocarcinoma of a WapTag1 female (77 weeks) (20 
). (C, D) HRAS male mammary tumour (11 weeks) showing prominent fluorescence and solid carcinoma histology (20 ); (E, F) Fluorescent multifocal tumours of a PyVT female (5 weeks) with solid carcinoma histology (20 ). Scale bar represents 1 mm.
Figure 2.
Photographic image of PpIX-stimulated red fluorescence in a metastatic tumour derived from a PyVT strain female (15 weeks) 1 h following 200 mg kg-1 ALA administration. (A) Brightfield image of isolated bronchial tree. (B) Red fluorescence detection of a metastatic tumour in the lung (arrow). (C) Representative H&E stained histological section of a tumour metastases from PyVT strain females (4 ).
Figure 3.
Digital image capture of PpIX-stimulated red fluorescence in early DCIS lesions of two WapTag1 females (41 and 45 weeks) 1 h following in vivo administration of 200 mg kg-1 ALA. (A, C) Red fluorescence detection of DCIS in the mammary gland. In (A) The white arrow indicates the region of DCIS, and the black arrow indicates accumulated proteinaceous fluid. (B, D) Corresponding H&E stained histological sections (20 ). Scale bar represents 0.5 mm.
Figure 4.
Flow cytometric measurement of digital mean fluorescence in PpIX-loaded tumour cells 1 h following in vivo administration of 200 mg kg-1 ALA (mean
s.e.). (A) Mammary tumour cells from HRAS males. (B) Normal mammary gland and mammary tumour cells from PyVT females.
