1. ¹Department of Biochemistry, Medical Centre for Postgraduate Education, Marymoncka 99, 01-813 Warsaw, Poland
2. ²Department of Pathology, Medical Centre for Postgraduate Education, Cegowska 80, 01-809 Warsaw, Poland
3. ³Department of Gastroenterology, Medical Centre for Postgraduate Education, Oncology Centre, M. Skodowska - Curie Memorial Institute, Roentgena 5, 02-781 Warsaw, Poland
4. ⁴Cell & Immunobiology Laboratory, Department of Medicine I, Medical University, Ratzeburger Allee 160, D-23538 Lübeck, Germany

Correspondence: B Czarnocka, E-mail: barbarac@cmkp.edu.pl

Received 1 January 2005; Revised 19 April 2005; Accepted 26 April 2005; Published online 7 June 2005.

Abstract

The Pendred syndrome gene (PDS) encodes a transmembrane protein, pendrin, which is expressed in follicular thyroid cells and participates in the apical iodide transport. Pendrin expression has been studied in various thyroid neoplasms by means of immunohistochemistry (IHC), Western blot and RT–quantitative real-time PCR. The expression was related to the functional activity of the thyroid tissue. Follicular cells of normal, nodular goitre and Graves' disease tissues express pendrin at the apical pole of the thyrocytes. In follicular adenomas, pendrin was detected in cell membranes and cytoplasm simultaneously in 10 out of 15 cases. Pendrin protein was detected in 73.3 and 76.7% of the follicular (FTC) and papillary (PTC) thyroid carcinomas, respectively, where pendrin was solely localised inside the cytoplasm. An extensive intracellular immunostaining of pendrin was observed in six out of 11 (54.5%) of positive FTCs and 19 out of 23 (82%) of PTCs. Focal reactivity was detected in one follicular- and three papillary carcinomas, whereas pendrin protein was absent in three of 15 FTC and four of 30 PTC; mRNA of pendrin was detected in 92.4% of thyroid tumours. The relative mRNA expression of pendrin was lower in cancers than in normal thyroid tissues (P<0.001). The pendrin protein level was found to parallel its mRNA expression, which was not, however, related to the tumour size and tumour stage. In conclusion, pendrin is expressed in the majority of differentiated thyroid tumours with high individual variability but its targeting to the apical cell membrane is affected.