FIGURES AND TABLES

Figure 1.

Unsupervised hierarchical clustering of 15 primary uterine cell lines (i.e., 10 USPC and five NEC). The cluster is colour coded using red for upregulation, green for downregulation, and black for median expression. Agglomerative clustering of genes is illustrated with dendrograms.

Figure 2.

Quantitative RT–PCR and microarray expression analysis of CDKN2A/p16, CDKN2A/p14ARF, claudin-3, claudin-4, GRB-7 and c-erbB2 genes differentially expressed between USPC and NEC. Quantitative RT-PCR data were highly correlated to the microarray data (P<0.001).

Figure 3.

Representative immunohistochemical staining for claudin-4 on two paraffin-embedded USPC specimens (B–C) and one NEC specimen (A). Normal endometrial cell 1 (upper panel) showed light membrane staining for claudin-4, while USPC 1 and USPC 3 showed heavy cytoplasmic and membranous staining for claudin-4 (middle and lower panel). Note the large difference in the size of USPC cells when compared to the control glandular epithelium of a menopausal women. Original magnification 400.

Figure 4.

Representative dose-dependent CPE-mediated cytotoxicity of primary USPC compared to positive control Vero cells or negative controls (i.e., NEC, fibroblasts, and monocytes) after 24 h exposure to scalar doses of CPE. VERO, positive control cells. Uterine serous papillary carcinoma-1 to USPC-3 primary uterine serous tumors. NEC, normal endometrial cells. Fibroblasts, normal human fibroblasts. Monocytes, normal mononuclear cells.

Table 1 - Characteristics of the patients.

Table 2 - Upregulated genes expressed at least five-fold higher in USPC compared with NEC.

Table 3 - Upregulated genes expressed at least 10-fold higher in NEC compared with USPC.