Increased frequency of the k-ras G12C mutation in MYH polyposis colorectal adenomas

doi:doi:10.1038/sj.bjc.6601747

British Journal of Cancer (2004) 90, 1591–1593. doi:10.1038/sj.bjc.6601747 www.bjcancer.com
Published online 30 March 2004

Increased frequency of the k-ras G12C mutation in MYH polyposis colorectal adenomas

S Jones¹, S Lambert¹, G T Williams², J M Best², J R Sampson¹ and J P Cheadle¹

¹Institute of Medical Genetics, University of Wales College of Medicine, Heath Park, Cardiff CF14 4XN, UK
²Department of Pathology, University of Wales College of Medicine, Heath Park, Cardiff CF14 4XN, UK

Correspondence: Dr JP Cheadle, E-mail: cheadlejp@cardiff.ac.uk

Received 17 September 2003; Accepted 4 February 2004; Published online 30 March 2004.

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Abstract

Colorectal tumours from MYH polyposis patients display an excess of somatic G : C T : A transversions in the adenomatous polyposis coli gene. Here, we identify k-ras mutations in nine out of 54 (16.7%) MYH polyposis tumours. Their presence was associated with increased dysplasia and tubulovillous morphology (P=0.005). G : C T : A transversions in k-ras were significantly more frequent in MYH polyposis adenomas than in sporadic or familial adenomatous polyposis-associated tumours (P less than or equal to 0.002), and all resulted in a glycine-to-cysteine substitution at codon 12.