FIGURES AND TABLES
FROM:
Role of lipid-mobilising factor (LMF) in protecting tumour cells from oxidative damage
P M Sanders and M J Tisdale
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Figure 1.
SDS–PAGE of LMF purified from human urine according to the protocol described in Materials and methods. Lane 1, MW markers; lane 2, LMF. Detection was by Coomassie brilliant blue stain.
Full figure and legend (73K)
Figure 2.
Immunoblot of hZAG (lanes 1–3) and hLMF (lanes 4–6) (10 
g) detected with polyclonal antibody to hZAG.
Figure 3.
Immunoblot of soluble extracts of MAC13 (lanes 1–3) and MAC16 (lanes 4–6) detected with monoclonal antibody to human ZAG.
Full figure and legend (42K)
Figure 4.
Immunoblot of UCP-2 expression in MAC13 cell line (A) in the presence of 0 (lane 1), 0.23 (lane 2), 0.35 (lane 3) and 0.58 (lane 4)
M LMF after 24 h incubation and (B) in the presence of 0 (lanes 1 and 6), 0.23 (lanes 2 and 7), 0.35 (lanes 3 and 8), 0.46 (lanes 4 and 9) and 0.58 (lanes 5 and 10)
M LMF for 24 h in the absence (lanes 1–5) or presence (lanes 6–10) of 10
M SR59230A. (C) Densitometric analysis of the blot shown in (B). The symbols 
are in the absence of SR59230A and 
in the presence; n=3. Differences from values in the presence of SR59230A are indicated as b, P<0.01.
Figure 5.
Effect of bleomycin alone on the growth of MAC13 cells (open boxes), or in the presence of 0.58
M LMF (hatched boxes), or 0.58
M LMF+10
M SR59230A (stippled boxes). Zinc-
2-glycoprotein alone was used at a concentration of 0.58
M. Total repeats n=3. Differences from values in the presence of bleomycin alone are indicated as c, P<0.001, white differences from bleomycin +ZAG are indicated as b, P<0.01, and d, P<0.001.
Figure 6.
Effect of hydrogen peroxide on the growth of MAC13 cells alone (open boxes) and in the presence of 0.58
M ZAG (hatched boxes). Zinc-2-glycoprotein alone was used at a concentration of 0.58
M. Total repeats n=3. Differences from values in the presence of hydrogen peroxide alone are indicated as a, P<0.05, and b, P<0.01.
Figure 7.
(A) Effect of paraquat on the growth of MAC13 cells alone (open boxes) and in the presence of 0.58
M LMF alone (hatched boxes) or with 10
M SR59230A (stippled boxes). Lipid-mobilising factor was used alone at a concentration of 0.58
M. Total repeats n=3. Differences from values in the presence of paraquat alone are indicated as a, P<0.05, and b, P<0.01, white differences from paraquat+LMF are indicated as c, P<0.001. (B) Levels of MDA in MAC13 cells after no treatment (C) or treatment with 0.1
M paraquat (P), 0.1
M paraquat+0.58
M ZAG (P+ZAG) or 0.1
M paraquat+0.58
M ZAG+10
M SR59230A (P+ZAG+SR); n=6. Differences from control are shown as a, P<0.05, while differences from 0.1
M paraquat are shown as b, P<0.001, and differences from P+ZAG are shown as c, P<0.001.
Figure 8.
Effect of chlorambucil on the growth of MAC13 cells alone (open boxes) and in the presence of 0.58
M LMF (hatched boxes). Lipid-mobilising factor was used alone at a concentration of 0.58
M. There were no significant differences between the two groups.
