FIGURES AND TABLES

Figure 1.

Analysis of a primary squamous cell carcinoma (SCC-1) by M-FISH: the metaphase spread stained with DAPI (A) and the M-FISH probe (B), an example of a translocation involving material originating from chromosomes 10, 2 and 11 (C) and the karyotype of the cell (D). The chromosome view shows (from left to right): the chromosome as it appears in the metaphase spread, the pseudocolours generated by the computer, a graph of the intensity of each fluorochrome along the chromosome axis, the individual colour planes (red, gold, green, aqua and far red), the composite image of all the colours and the chromosome stained with DAPI. This metaphase contains 51 chromosomes and is one of the seven spreads used in compiling the composite karyotype of the sample (see Table 2).

Figure 2.

Graphical representation of the total number of interchromosomal translocations for each chromosome observed in the composite karyotypes for four primary NSCLC samples and two cell lines. The dashed line indicates an arbitary cutoff between the six chromosomes, which appear to be frequently involved in translocations and those which are involved less often.

Table 1 - Characteristics and colcemid treatment conditions of the four primary NSCLC samples.

Table 2 - Composite karyotype for each tumour sample and cell line.

Table 3 - 17 translocations that were observed in more than one sample by M-FISH and the number of times they were observed primary tumours and cell lines.