TABLE 1
FROM:
Modifying an immunogenic epitope on a therapeutic protein: a step towards an improved system for antibody-directed enzyme prodrug therapy (ADEPT)
A Mayer, S K Sharma, B Tolner, N P Minton, D Purdy, P Amlot, G Tharakan, R H J Begent and K A Chester
BACK TO ARTICLETable 1. Binding of biotinylated CM79 to MFECP, MFEdmCP and MFECPHis
| Fusion protein | CM79 bindinga (%) |
|---|---|
| MFECP (no tag) | 100 |
| MFEdmCP (no tag) | 0.8b |
| MFECPHis (His-tag) | 54b |
| MFEdmCPHis (His-tag) | 0.9b |
a Measured by ELISA on CEA-coated plates. Binding of biotinylated CM79 was detected by Extravidin AP.
b Binding of MFEdmCP, MFECPHis and MFEdmCPHis expressed as percentage of binding of MFECP.
