TABLE 2

FROM:

Kallikrein gene downregulation in breast cancer

G M Yousef, G M Yacoub, M-E Polymeris, C Popalis, A Soosaipillai and E P Diamandis

Table 2. Primers and conditions used for PCR analysis

Gene	Sequencea	Product lengthb	T a c	Number of cycles
KLK5	GTCACCAGTTTATGAATCTGGGC	328	60	35
	GGCGCAGAACATGGTGTCATC
KLK6	GAAGCTGATGGTGGTGCTGAGTCTG	454	60	35
	GTCAGGGAAATCACCATCTGCTGTC
KLK8	GCCTTGTTCCAGGGCCAGC	416	65	35
	GCATCCTCACACTTCTTCTGGG
KLK10	GGAAACAAGCCACTGTGGGC	468	60	35
	GAGGATGCCTTGGAGGGTCTC
KLK12	GAGCAGATCCGGCACAGCGG	425	69	35
	TCTTGTCCACAGGGCCCCACAG
	GGCAGGGCGCAGCGCTCC
KLK15	CTACGGACCACGTCTCGGGTC	459	65	35
	GACACCAGGCTTGGTGGTGTTG
-actin	ATCTGGCACCACACCTTCTA	835	62	35
	CGTCATACTCCTGCTTGCTG

^a All sequences are mentioned from the 5' to 3' direction.

^b In base pairs.

^c Annealing temperature.