FIGURE 3
FROM:
Increased expression of the ubiquitin – proteasome pathway in murine myotubes by proteolysis-inducing factor (PIF) is associated with activation of the transcription factor NF-
B
A S Whitehouse and M J Tisdale
BACK TO ARTICLEFigure 3.
Western blot analysis of 20S proteasome 
-subunit expression (A) or (C) the 19S subunit, p42 in C2C12 myotubes 24 h after addition of PIF alone at 0 nM (lane 1), 2 nM (lane 2), 4 nM (lane 3), 8 nM (lane 4), 16 nM (lane 5) or 40 nM (lane 6) or after pretreatment with 50 
M EPA for 2 h and subsequent treatment with PIF at 0 nM (lane 7), 2 nM (lane 8), 4 nM (lane 9), 8 nM (lane 10), 16 nM (lane 11) and 40 nM (lane 12). (B) Densitometric analysis of the blot shown in (A), n=2. (D) Densitometric analysis of the blot shown in (C), n=2. Values shown are in the absence (black boxes) or presence (stippled boxes) of 50
M EPA. Differences from control are shown as a, P<0.001, while differences from PIF-treated cells are shown as b, P<0.001.
