1. ¹Department of Radiation Oncology, Dokuz Eylül University Medical School, Inciraltu, Izmir 35340, Turkey
2. ²Department of General Surgery, Dokuz Eylül University Medical School, Izmir 35340, Turkey
3. ³Department of Pathology, Dokuz Eylül University Medical School, Izmir 35340, Turkey
4. ⁴Tumour Metabolism and Therapeutics Group, School of Pharmacy and Chemistry, John Moores University, Liverpool L3 3AF, UK
5. ⁵Academic Department of Radiation Oncology, Christie Hospital, Manchester M20 4BX, UK

Correspondence: Dr R Cooper, E-mail: rachel.cooper@deu.edu.tr

Received 7 March 2003; Revised 19 June 2003; Accepted 24 June 2003.

Abstract

The aim of the study is to evaluate the pattern and level of expression of glucose transporter-1 (GLUT-1) in rectal carcinoma in relation to outcome as a potential surrogate marker of tumour hypoxia. Formalin-fixed tumour sections from 43 patients with rectal carcinoma, who had undergone radical resection with curative intent, were immunohistochemically stained for GLUT-1. A mean of three sections per tumour (range 1–12) were examined. Each section was semiquantitatively scored; 0, no staining; 1, <10%; 2, 10–50%; 3, >50% and a score given for the whole section, the superficial (luminal) and deep (mural) part of the tumour. Staining was seen in 70% of tumours. Increased staining was noted adjacent to necrosis and ulceration. A diffuse and patchy pattern of staining, with and without colocalisation to necrosis was seen. Patients with high GLUT-1-expressing tumours (score 3 vs 0–2) had a significantly poorer overall survival (P=0.041), which was associated with poorer metastasis-free survival with no difference in local control. No significant correlation was seen with other prognostic factors. There was a strong correlation between the score for the superficial and deep parts of the tumour (r=0.81), but a significant relationship with outcome was only found in the deep part (P=0.003 vs P=0.46). In conclusion, increased GLUT-1 expression in rectal tumours was an adverse prognostic factor and is worth further evaluation as a predictive marker of response to therapy.