FIGURES AND TABLES
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Rapid detection of allelic losses in brain tumours using microsatellite repeat markers and high-performance liquid chromatography
O B Chernova, G H Barnett and J K Cowell
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Figure 1.
Comparison of LOH analysis using DNA-HPLC and denaturing polyacrylamide gel electrophoresis. Examples from five tumours using marker D1S551 are shown. DNA from peripheral blood (B) or tumour (T) were analysed by both methods. Electrophoresis results are shown on the right. The relevant portions of the corresponding HPLC elution profiles are shown on the left in each example. Peaks 2 and 3 represent the two allelic homoduplexes of D1S551 in normal DNA from individuals heterozygous for the marker. Peak 1 represents a heteroduplex with shorter retention time. Tumour ccf 117 is constitutionally homozygous and shows only one peak. Tumour ccf 12 is heterozygous, but the two alleles differ in size by only four base pairs and so the two allelic peaks in this case do not resolve as well as in the other examples shown. In the three tumours showing LOH, the presence of a single peak in the tumour indicating the retained allele together with the loss of the heteroduplex peak can be clearly seen. The elution time (min) is represented on the x-axis, and the ultraviolet absorbance at 260 nm is represented on the y-axis (in 
V).
Figure 2.
High-performance liquid chromatography detection of LOH in glial tumours. Representative chromatograms from seven different tumour (T)/normal (B) comparisons are shown. Many of the markers show the clear results described for marker D1S551. Two markers, D1S430 and D19S572, show evidence of stutter bands in the PCR products as seen by the minor peaks flanking the main peak. Even despite the extra 'noise' seen in the chromatogram, it is still relatively easy to identify LOH in these cases by the presence of only a single main peak.
Full figure and legend (55K)
