FIGURES AND TABLES

Figure 1.

Enzyme characterisation of primary prostate epithelial cells and the prostate cell lines grown with BMS. Immunocytochemical staining showing typical colonies of cytokeratin (CK) positive (DAB brown staining) and fluorescent dual staining of epithelial cells grown in BMS co-culture (each picture is unrelated). Enzyme stained photographs are shown as dual fluorescence: red=cytokeratin; green=location of either uPA, MMP-1 or MMP-7; yellow=double staining; blue=DAPI nuclear counterstain (n=5). All negative controls were black. Arrows indicate retracted stromal edge.

Figure 2.

Serial sections of a prostatic bone marrow metastasis. Typical examples of the enzyme staining found from seven samples (see Table 2). Each photograph shows the same region of sample immunohistochemically (DAB) labelled for: (A) negative control (haematoxylin), (B) cytokeratin indicating the colonies of epithelial cells within the bone marrow space, (C) MMP-1, (D) MMP-7 and (E) uPA.

Figure 3.

Antibody inhibition of epithelial colony area (%). A comparison of the inhibition of prostate epithelial colony area in the presence of enzyme antibodies was made for a variety of primary epithelial samples. Typical immunocytochemical (DAB) cytokeratin staining of a BPH epithelial cell/BMS co-culture in the presence of non-specific mouse IgG1, anti-MMP-1, anti-MMP-7 and anti-uPA antibodies (2 g day^-1) after 7 days in culture is shown in (A). Median area of >100 colonies per sample were calculated and inhibition was calculated as a per cent of the control area. Median percentage inhibition is represented by the bar: (B) CaP, MMP-1 and MMP-7 (n=6), uPA n=4; (C) BPH, MMP-1 and MMP-7 (n=7), uPA (n=3).

Table 1 - Immunocytochemical (DAB) staining for the proteolytic enzymes in prostate epithelial cells derived from patients with either CaP or BPH and in the PC-3 and PNT2-C2 cell lines grown either on plastic or together with BMS as a co-culture.