Regular Article

British Journal of Cancer (2000) 83, 1468–1472. doi:10.1054/bjoc.2000.1464 www.bjcancer.com
Published online 14 November 2000

Expression of the novel tumour suppressor p33ING1 is independent of p53

K-J J Cheung Jr1, J A Bush1, W Jia2 and G Li1

  1. 1Department of Medicine, Division of Dermatology, Vancouver, British Columbia, V6H 3Z6, Canada
  2. 2Department of Surgery, University of British Columbia and Vancouver Hospital and Sciences Centre, Vancouver, British Columbia, V6H 3Z6, Canada

Correspondence: G Li, E-mail: gangli@interchange.ubc.ca

Received 22 March 2000; Accepted 18 July 2000.

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Abstract

A recently cloned tumour suppressor candidate, p33ING1, has been shown in vitro to collaborate with p53 to execute growth arrest and apoptosis. However, it is unclear as to how the expression of ING1 is regulated in normal and stress conditions. Using a p53-knockout mouse model, we investigated if the expression of ING1 was dependent on p53. We found that there was no difference in ING1 mRNA and protein levels between p53+/+ and p53–/– murine organs. In addition, when normal human epithelial keratinocytes (NHEK) and a keratinocyte cell line, HaCaT, which lacks wild-type p53 function, were exposed to UVB irradiation, the expression levels of ING1 were elevated in both NHEK and HaCaT cells. It is interesting, however, that UVB irradiation did not induce ING1 expression in dermal fibroblasts isolated from p53+/+ and p53–/– mice. Based on our findings, we therefore conclude that the expression of ING1 is independent of p53 status. UV induction of ING1 in keratinocytes suggests that ING1 may play a role in cellular stress response and skin carcinogenesis. © 2000 Cancer Research Campaign http://www.bjcancer.com

Keywords:

p33ING1, p53, in vivo expression, tumour suppressor gene, UV irradiation

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