Short Communication

British Journal of Cancer (2000) 82, 1218–1222. doi:10.1054/bjoc.1999.1066 www.bjcancer.com
Published online 22 February 2000

Analysis of pilocytic astrocytoma by comparative genomic hybridization

D Sanoudou1, O Tingby3,4, M A Ferguson-Smith2, V P Collins1,3,4 and N Coleman1,3

  1. 1Departments of Pathology
  2. Clinical Veterinary Medicine, University of Cambridge, Cambridge, UK
  3. 3Department of Histopathology, Addenbrooke's Hospital, Cambridge, UK
  4. 4Institution of Oncology and Pathology, Karolinska Institute, S-17176 Stockholm, Sweden

Received 7 July 1999; Revised 9 November 1999; Accepted 11 November 1999.

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Abstract

Very little is known about genetic abnormalities involved in the development of pilocytic astrocytoma, the most frequently occurring brain tumour of childhood. We have analysed 48 pilocytic astrocytoma specimens using comparative genomic hybridization. Only five of 41 tumours from children showed abnormalities detectable by comparative genomic hybridization, and in each case this represented gain of a single chromosome. Interestingly, two of seven tumours from adults showed abnormalities, which were multiple and relatively complex. Six of the seven tumours showing abnormalities were from female patients (two adults and four children). The most frequently detectable abnormality was gain of 9q34.1-qter, which was present in three cases (two adult and one paediatric). © 2000 Cancer Research Campaign

Keywords:

pilocytic astrocytoma, glioma, comparative genomic hybridization

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References

  1. Agamanolis DP and Malone AM (1995) Chromosomal abnormalities in 47 pediatric brain tumors. Cancer Genet Cytogenet 81: 125–134 | Article | PubMed | ChemPort |
  2. al-Sarraj S, Bridges LR, Cawkwell L, Lewis FA and Quirke P (1995) p53 allelic imbalance in astrocytoma detected using fluorescent PCR of microsatellite repeat polymorphisms. Neuropathol Appl Neurobiol 21: 344–351
  3. Bhattacharjee MB, Armstrong DD, Vogel H and Cooley LD (1997) Cytogenetic analysis of 120 primary pediatric brain tumors and literature review. Cancer Genet Cytogenet 97: 39–53 | Article | PubMed | ChemPort |
  4. Bigner SH, McLendon RE, Fuchs H, McKeever PE and Friedman HS (1997) Chromosomal characteristics of childhood brain tumors. Cancer Genet Cytogenet 97: 125–134 | Article | PubMed | ChemPort |
  5. Debiec-Rychter M, Alwasiak J, Liberski PP, Nedoszytko B, Babinska M, Mrozek K, Imielinski B, Borowska-Lehman J and Limon J (1995) Accumulation of chromosomal changes in human glioma progression. A cytogenetic study of 50 cases. Cancer Genet Cytogenet 85: 61–67 | Article | PubMed | ChemPort |
  6. Duerr EM, Rollbrocker B, Hayashi Y, Peters N, Meyer-Puttlitz B, Louis DN, Schramm J, Wiestler OD, Parsons R, Eng C and von Deimling A (1998) PTEN mutations in gliomas and glioneuronal tumors. Oncogene 16: 2259–2264 | Article | PubMed | ISI | ChemPort |
  7. Ganju V, Jenkins RB, O'Fallon JR, Scheithauer BW, Ransom DT, Katzmann JA and Kimmel DW (1994) Prognostic factors in gliomas. A multivariate analysis of clinical, pathologic, flow cytometric, cytogenetic, and molecular markers. Cancer 74: 920–927
  8. Giannini C and Scheithauer BW (1997) Classification and grading of low-grade astrocytic tumors in children. Brain Pathol 7: 785–798
  9. Jenkins RB, Kimmel DW, Moertel CA, Schultz CG, Scheithauer BW, Kelly PJ and Dewald GW (1989) A cytogenetic study of 53 human gliomas. Cancer Genet Cytogenet 39: 253–279 | Article | PubMed | ISI | ChemPort |
  10. Johansson B, Heim S, Mandahl N, Mertens F and Mitelman F (1993) Trisomy 7 in nonneoplastic cells. Genes Chromosomes Cancer 6: 199–205
  11. Kallioniemi A, Kallioniemi OP, Sudar D, Rutovitz D, Gray JW, Waldman F and Pinkel D (1992) Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors. Science 258: 818–821 | Article | PubMed | ISI | ChemPort |
  12. Kallioniemi OP, Kallioniemi A, Piper J, Isola J, Waldman FM, Gray JW and Pinkel D (1994) Optimizing comparative genomic hybridization for analysis of DNA sequence copy number changes in solid tumors. Genes Chromosomes Cancer 10: 231–243 | Article | PubMed | ISI | ChemPort |
  13. Karnes PS, Tran TN, Cui MY, Raffel C, Gilles FH, Barranger JA and Ying KL (1992) Cytogenetic analysis of 39 pediatric central nervous system tumors. Cancer Genet Cytogenet 59: 12–19 | Article | PubMed | ChemPort |
  14. Lang FF, Miller DC, Pisharody S, Koslow M and Newcomb EW (1994) High frequency of p53 protein accumulation without p53 gene mutation in human juvenile pilocytic, low grade and anaplastic astrocytomas. Oncogene 9: 949–954 | PubMed | ISI | ChemPort |
  15. Liu L, Ichimura K, Pettersson EH and Collins VP (1998) Chromosome 7 rearrangements in glioblastoma; loci adjacent to EGFR are independently amplified. J Neuropathol Exp Neurol,
  16. Mathew P, Look T, Luo X, Ashmun R, Nash M, Gajjar A, Walter A, Kun L and Heideman RL (1996) DNA index of glial tumors in children. Correlation with tumor grade and prognosis. Cancer 78: 881–886
  17. Patt S, Gries H, Giraldo M, Cervos-Navarro J, Martin H, Janisch W and Brockmoller J (1996) p53 gene mutations in human astrocytic brain tumors including pilocytic astrocytomas. Human Pathol 27: 586–589
  18. Phelan CM, Liu L, Ruttledge MH, Muntzning K, Ridderheim PA and Collins VP (1995) Chromosome 17 abnormalities and lack of TP53 mutations in paediatric central nervous system tumours. Hum Genet 96: 684–690
  19. Platten M, Giordano MJ, Dirven CM, Gutmann DH and Louis DN (1996) Up-regulation of specific NF 1 gene transcripts in sporadic pilocytic astrocytomas. Am J Pathol 149: 621–627 | PubMed | ChemPort |
  20. Ransom DT, Ritland SR, Kimmel DW, Moertel CA, Dahl RJ, Scheithauer BW, Kelly PJ and Jenkins RB (1992) Cytogenetic and loss of heterozygosity studies in ependymomas, pilocytic astrocytomas, and oligodendrogliomas. Genes Chromosomes Cancer 5: 348–356 | Article | PubMed | ChemPort |
  21. Scheurlen WG and Senf L (1995) Analysis of the gap-related domain of the neurofibromatosis type 1 (nf1) gene in childhood brain tumors. Int J Cancer 64: 234–238
  22. Telenius H, Pelmear AH, Tunnacliffe A, Carter NP, Behmel A, Ferguson-Smith MA, Nordenskjold M, Pfragner R and Ponder BA (1992) Cytogenetic analysis by chromosome painting using DOP-PCR amplified flow-sorted chromosomes. Genes Chromosomes Cancer 4: 257–263 | Article | PubMed | ISI | ChemPort |
  23. Thiel G, Losanowa T, Kintzel D, Nisch G, Martin H, Vorpahl K and Witkowski R (1992) Karyotypes in 90 human gliomas. Cancer Genet Cytogenet 58: 109–120 | Article | PubMed | ISI | ChemPort |
  24. Tomlinson FH, Scheithauer BW, Hayostek CJ, Parisi JE, Meyer FB, Shaw EG, Weiland TL, Katzmann JA and Jack CR Jr (1994) The significance of atypia and histologic malignancy in pilocytic astrocytoma of the cerebellum: a clinicopathologic and flow cytometric study. J Child Neurol 9: 301–310 | PubMed | ISI | ChemPort |
  25. von Deimling A, Louis DN, Menon AG, von AK, Petersen I, Ellison D, Wiestler OD and Seizinger BR (1993) Deletions on the long arm of chromosome 17 in pilocytic astrocytoma. Acta Neuropathol (Berl) 86: 81–85
  26. White FV, Anthony DC, Yunis EJ, Tarbell NJ, Scott RM and Schofield DE (1995) Nonrandom chromosomal gains in pilocytic astrocytomas of childhood. Hum Pathol 26: 979–986 | Article | PubMed | ChemPort |
  27. Willert JR, Daneshvar L, Sheffield VC and Cogen PH (1995) Deletion of chromosome arm 17p DNA sequences in pediatric high-grade and juvenile pilocytic astrocytomas. Genes Chromosomes Cancer 12: 165–172
  28. Zattara-Cannoni H, Gambarelli D, Lena G, Dufour H, Choux M, Grisoli F and Vagner-Capodano AM (1998) Are juvenile pilocytic astrocytomas benign tumors? A cytogenetic study in 24 cases. Cancer Genet Cytogenet 104: 157–160 | Article | PubMed | ChemPort |