Regular Article

British Journal of Cancer (2000) 82, 1808–1813. doi:10.1054/bjoc.2000.1111 www.bjcancer.com
Published online 4 May 2000

Human tumour-associated cell adhesion protein MN/CA IX: identification of M75 epitope and of the region mediating cell adhesion

J Závada1, Z Závadová1, J Pastorek2, Z Biesová2, J Jez breveek3 and J Velek3

  1. 1Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Flemingovo nam. 2, 16637 Prague, Czech Republic
  2. 2Institute of Virology, Slovak Academy of Sciences, 84246 Bratislava, Slovakia
  3. 3Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, 16610 Prague

Received 16 September 1999; Accepted 27 January 2000.

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Abstract

MN/CA IX is a cell surface protein, strongly associated with several types of human carcinomas. It exerts activity of carbonic anhydrase and capacity of binding to cell surface receptors. In the present work, we used affinity purified MN/CA IX protein to demonstrate that the cells adhere to immobilized MN/CA IX and that the monoclonal antibody M75 abrogates cell attachment to MN/CA IX. Using synthetic oligopeptides, we identified M75 epitope and located it in the proteoglycan domain, which contains a sixfold tandem repeat of six amino acids GEEDLP. From phage display library of random heptapeptides we identified and chemically synthesized those which compete for the epitope with M75 and inhibit adhesion of cells to MN/CA IX. These heptapeptides might serve as lead compounds for drug design. © 2000 Cancer Research Campaign

Keywords:

cell adhesion molecules, carbonic anhydrase, tumour immunology, phage display, drug design

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