Regular Article

British Journal of Cancer (1999) 79, 472–477. doi:10.1038/sj.bjc.6690073 www.bjcancer.com
Published online 14 January 1999

Expression of tissue factor in non-small-cell lung cancers and its relationship to metastasis

M Sawada1,2, S Miyake1,2, S Ohdama1,2, O Matsubara3, S Masuda4, K Yakumaru5 and Y Yoshizawa1,2

  1. 1Department of Pulmonary Medicine, Tokyo Medical and Dental University, 1-5-45, Bunkyo-ku, Tokyo 113-8519, Japan
  2. 2First Department of Internal Medicine, Tokyo Medical and Dental University, 1-5-45 Bunkyo-ku, Tokyo 113-8519, Japan
  3. 3Department of Pathology, National Defense Medical College, 3-2 Tokorozawa, Saitama, 359-0042, Japan
  4. 4Department of Surgery, Tokyo Teishin Hospital, 2-14-23 Chiyoda-ku, Tokyo 102-0071, Japan
  5. 5Department of Pathology, Tokyo Teishin Hospital, 2-14-23 Chiyoda-ku, Tokyo 102-0071, Japan

Correspondence: Y Yoshizawa, Department of Pulmonary Medicine, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8519, Japan

Received 1 September 1997; Revised 8 April 1998; Accepted 16 April 1998.

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Abstract

Tissue factor (TF) is an initiator of the extrinsic cascade of blood coagulation. Although recent studies have revealed a relationship between metastatic properties and TF expression in some neoplastic cells, the significance of TF in lung cancer, especially in non-small-cell lung cancer (NSCLC), is still unclear. In this study, TF was detected in NSCLC cell lines by functional study, Western blot analysis and immunocytochemical staining. TF levels in eight NSCLC cell lines were also quantitated by enzyme-linked immunosorbent assay (ELISA), and TF expression was evaluated in 55 specimens of surgically resected NSCLCs. NSCLC cell lines derived from metastatic lesions produced high levels of TF (48.3 plusminus 23.5 ng 10–6 cells, mean plusminus s.e.m.), whereas those derived from primary lesions produced low levels of TF (0.2 plusminus 0.1 ng 10–6 cells). Immunohistochemical studies disclosed significantly stronger staining for TF in cells from NSCLC patients with metastasis than in those without metastasis. Among the 28 patients with metastasis, ten were strongly positive, 16 were moderately positive and two were negative for TF. In contrast, among the 27 patients without metastasis, only two were strongly positive, 18 were moderately positive and seven were negative for TF. Therefore, malignant cells from patients with lung cancer produce various levels of TF, and TF may play an important role in the metastatic process.

Keywords:

tissue factor, lung cancer, non-small-cell lung cancer, metastasis

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