Abstract
The establishment and characterisation (morphology, ultrastructure, tumourigenicity) of six cell lines from primary human colorectal adenocarcinomas is described. These lines were established from surgical specimens, from 49 unselected patients, without the use of 'feeder' cells, 'conditioned' medium or passage of cells in nude mice. The six cell lines exhibit considerable variation in morphology, CEA secretion and tumourigenicity in nude mice. At least two of the lines retain some of the differentiated characteristics of colorectal epithelium.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 24 print issues and online access
$259.00 per year
only $10.79 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
Rights and permissions
About this article
Cite this article
Kirkland, S., Bailey, I. Establishment and characterisation of six human colorectal adenocarcinoma cell lines. Br J Cancer 53, 779–785 (1986). https://doi.org/10.1038/bjc.1986.132
Issue Date:
DOI: https://doi.org/10.1038/bjc.1986.132
This article is cited by
-
Explant culture of sarcoma patients' tissue
Laboratory Investigation (2016)
-
Culture of low passage colorectal cancer cells and demonstration of variation in selected tumour marker expression
Cytotechnology (2014)
-
Rectal washout eliminates exfoliated malignant cells
Diseases of the Colon & Rectum (1998)
-
Establishment and characterization of a human colon cancer cell line, OUMS-23, from a patient with familial adenomatous polyposis
Journal of Cancer Research and Clinical Oncology (1996)
-
Retention of tissue‐specific phenotype in a panel of colon carcinoma cell lines: Relationship to correlates
Immunology & Cell Biology (1992)