Maternal immune activation results in complex microglial transcriptome signature in the adult offspring that is reversed by minocycline treatment

Mattei, D; Ivanov, A; Ferrai, C; Jordan, P; Guneykaya, D; Buonfiglioli, A; Schaafsma, W; Przanowski, P; Deuther-Conrad, W; Brust, P; Hesse, S; Patt, M; Sabri, O; Ross, T L; Eggen, B J L; Boddeke, E W G M; Kaminska, B; Beule, D; Pombo, A; Kettenmann, H; Wolf, S A

doi:10.1038/tp.2017.80

Download PDF

Original Article
Open access
Published: 09 May 2017

Maternal immune activation results in complex microglial transcriptome signature in the adult offspring that is reversed by minocycline treatment

D Mattei¹^na1,
A Ivanov^2,3^na1,
C Ferrai⁴,
P Jordan¹,
D Guneykaya¹,
A Buonfiglioli^1,5,
W Schaafsma⁶,
P Przanowski⁷,
W Deuther-Conrad⁸,
P Brust⁸,
S Hesse^9,10,
M Patt⁹,
O Sabri⁹,
T L Ross¹¹,
B J L Eggen⁶,
E W G M Boddeke⁶,
B Kaminska⁷,
D Beule^2,12,
A Pombo⁴,
H Kettenmann¹ &
…
S A Wolf¹

Translational Psychiatry volume 7, page e1120 (2017)Cite this article

7840 Accesses
148 Citations
7 Altmetric
Metrics details

Subjects

Abstract

Maternal immune activation (MIA) during pregnancy has been linked to an increased risk of developing psychiatric pathologies in later life. This link may be bridged by a defective microglial phenotype in the offspring induced by MIA, as microglia have key roles in the development and maintenance of neuronal signaling in the central nervous system. The beneficial effects of the immunomodulatory treatment with minocycline on schizophrenic patients are consistent with this hypothesis. Using the MIA mouse model, we found an altered microglial transcriptome and phagocytic function in the adult offspring accompanied by behavioral abnormalities. The changes in microglial phagocytosis on a functional and transcriptional level were similar to those observed in a mouse model of Alzheimer’s disease hinting to a related microglial phenotype in neurodegenerative and psychiatric disorders. Minocycline treatment of adult MIA offspring reverted completely the transcriptional, functional and behavioral deficits, highlighting the potential benefits of therapeutic targeting of microglia in psychiatric disorders.

Maternal immune activation alters fetal and neonatal microglia phenotype and disrupts neurogenesis in mice

Article 13 August 2022

Maternal immune activation induces sustained changes in fetal microglia motility

Article Open access 07 December 2020

Modulating microglia activation prevents maternal immune activation induced schizophrenia-relevant behavior phenotypes via arginase 1 in the dentate gyrus

Article 29 June 2020

Introduction

In recent years, microglia as the brain’s intrinsic immune cells have emerged as having new roles in the pathophysiology of psychiatric disorders such as schizophrenia.¹ Signs of their involvement stem from human postmortem and positron emission tomography studies indicating altered microglial cell density and binding to the translocator protein (TSPO) in the brains of a subset of schizophrenic patients.^{2, 3, 4} Microglia are able to phagocytose synaptic material in the process of synaptic pruning, which is essential for the proper development and maintenance of brain circuitry.⁵ Their phagocytic activity also controls the pool of neuronal progenitor cells during development and in adulthood.^{6, 7} As schizophrenia is a disorder with prominent abnormalities in synaptogenesis,⁸ neurogenesis^{9, 10} and neuronal transmission,^{8, 11} it is plausible that microglial dysfunction results in aberrant synaptogenesis and neurogenesis in the developing central nervous system, contributing to the development and progression of schizophrenia. Epidemiological studies have linked prenatal influenza infections with an increased risk of developing schizophrenia in the offspring.^{12, 13} Injection of the viral mimic polyinosinic:polycytidilic acid (Poly(I:C)) to pregnant rodent dams has been shown to induce a series of behavioral, morphological, cytoarchitectural and biochemical changes in the brain of adult offspring, which are highly comparable to the abnormalities observed in human schizophrenic patients.^{14, 15} The maternal immune activation (MIA) in humans and animal models alike could impact on the fetal microglia and result in functional changes that are retained in adulthood.

Several clinical case reports have suggested that minocycline alleviates mainly negative symptoms in schizophrenic patients. Subsequent clinical trials were initiated to test the potential of the drug as an add-on therapy for schizophrenia. In spite of the accumulating data showing beneficial effects of minocycline treatment on both human patients^{16, 17, 18, 19, 20, 21, 22} and in animal models of schizophrenia,^{23, 24} a mechanistic insight into the action of minocycline is lacking at present. It is suggested that minocycline interferes with microglial activation; however, its actions may be nonselective to microglial function. Chronic inflammation and, in particular, MIA could be a major factor that contributes to the development of neurodegenerative disorders such as Alzheimer’s disease.^{25, 26, 27} Lahiri et al.²⁸ proposed a 'Latent Early-life Associated Regulation' model, postulating that latent changes in the expression of specific genes initially primed at the developmental stage of life. However, the involvement of microglia in neuropsychiatric diseases is unclear.

Therefore, using the maternal immune activation model, we investigated the effects of minocycline treatment on the phenotype of hippocampal microglia derived from adult male offspring. We also compared these data sets to the ones obtained from an Alzheimer’s disease mouse model. We focused here on the hippocampus as it is a structure particularly affected in schizophrenia, and its malfunction contributes to the appearance of positive, negative and cognitive symptoms.^{29, 30, 31, 32}

Materials and methods

Animals

The C57BL/6 mice were handled according to governmental (LaGeSo) and internal (MDC) rules and regulations (animal protocol number G O196/12). Day one of pregnancy was calculated from the day of appearance of a vaginal plug. Pregnant dams were injected once intraperitoneally with either Polyinosinic:polycytidylic acid (5 mg kg⁻¹ Poly(I:C) VacciGrade, Invivogen, San Diego, CA, USA) or 0.9% saline solution at gestational day 15 (G15, Figure 1a). Despite its advantages, there are also limitations to the Poly(I:C)-based MIA animal paradigm. Apart from the ‘traditional’, general concerns relating to the usage of rodent systems to model complex neuropsychiatric disorders, which present with alterations of inherently ‘human’ behavioral features, there are also experimental constrains of Poly(I:C)-assisted MIA that need to be considered. The major drawback of using immunogenic manipulations based upon Poly(I:C), rather than live pathogens, is the—albeit well-characterized—limited set of immune responses elicited. Comparatively, stimulating the maternal immune system by administration of a real virus (such as influenza), leads to a much broader activation of not only the innate, but also the acquired immune system, thus more closely resembling the situation following infection during pregnancy in humans. To ensure a least of five animals per group for every set of experiments, an average of 10 dams per group (Poly(I:C) and saline injected) was used. Male offspring were weaned at postnatal day 21 (P21) and they were caged in a random manner so as to avoid confounding litter effects. The mice were left undisturbed until the day of behavioral phenotyping with the prepulse inhibition (PPI) test at P60. The latter was used as a selection criteria so that only the Poly(I:C) mice displaying deficits in PPI were selected for further experiments. In Supplementary Figure 3, we show all animals tested with PPI and indicate the ones that were included for further analysis. The mice were kept in an animal facility with 12 h of light and dark cycle with food and water ad libitum.

Minocycline treatment

Minocycline (Sigma-Aldrich, Munich, Germany) was dissolved in drinking water at 3 mg kg⁻¹ per day assuming an average consumption of 5 ml water per day per animal. The dose was selected on the basis of the oral dosage given to schizophrenic subjects in clinical trials (200 mg per day assuming 70 kg weight, reviewed in ref. 33). Minocycline treatment was started at postnatal day (P)70–P80 after the assessment of the baseline behavioral deficits, and was carried out for 5 weeks and throughout the behavioral testing period (Figure 1a). On treatment, the mice were randomly divided into the following groups: (Ctr), Controls—offspring from NaCl-treated dams; Poly—offspring of Poly(I:C)-treated dams; and Poly/Mino—offspring from Poly(I:C)-treated dams that received minocycline as adults. We have previously reported that the minocycline treatment given to control animals did not affect the sensorimotor gating, adult hippocampal neurogenesis, microglial cell density and cytokine expression pattern.²³

Behavioral testing

Previous work conducted on Poly(I:C) offspring showed that they start to display behavioral deficits at the switch between adolescence and early adulthood, as it is the case in human schizophrenic patients (reviewed in ref. 34). This age corresponds in mice to P60. We therefore started the behavioral battery tests at P60. The behavioral testing was performed by a person blind to the experimental groups.

Prepulse inhibition

The PPI of startle reflex was assessed using a standard startle chamber (Figure 1b, SR-Lab, San Diego Instruments, San Diego, CA, USA) according to the manufacturer's protocol. The session starts with 5 min habituation where the mouse is exposed to white noise at 65 decibel (dB). Afterwards, a pseudorandomized session started with 10 different types of trials: 8 acoustic startle pulse alone (120 dB) and 10 different prepulse followed by pulse trials were applied in which either 69, 73 or 81 dB stimuli were presented 100 ms before the pulse. Intertrial intervals of a random duration of 10–25 s (65 dB white noise) separated the trials. In the prepulse plus startle pulse trials, the amount of PPI is measured and expressed as percentage of the basal startle response (startle pulse alone).

Novel object recognition test

The test was performed in a square-shaped open-field box with high walls (72 × 72 × 33 cm, Figure 1d). The test was performed as described elsewhere, but with some modifications.³⁵ The test starts with a 5 min habituation session. After an intertrial interval of 5 min, the mouse is allowed to explore two different objects for 5 min. After an intertrial interval of 5 min, the mouse was allowed to explore the same arena for 3 min containing one old and one novel object (Figure 1d). The measurements were performed through the Anymaze software (ANY-maze 4.50, Stoelting Europe, Dublin, Ireland). To assess the detection of novelty, we calculated the percentage of time spent by the mice exploring the novel object (NO) as compared with the total time spent in exploring the new and old object (OB):

(NO_{ExplorationTime}/(NO_{ExplorationTime}+OB_{ExplorationTime})) × 100. The open field and the objects were cleaned with 5% ethanol after each mouse was tested, to avoid olfactory cues.

Sociability test

To assess the social behavior of the mice, the sociability test was performed in a tripartite chamber, composed of three chambers of equal size (20 × 40 cm, Figure 1f). After habituation for 5 min in the middle chamber, two cylindrical cages, one empty and one containing a stranger mouse were placed in the lateral chambers. The mouse was given then free access to all chambers for a total of 10 min (ANY-maze 4.50, Stoelting Europe). The preference for the stranger mouse (S) toward the empty cage (E) was assessed by calculating the percentage of time spent making contact with the stranger mouse over the sum of time spent with both the S and the E: (S_{TimeMakingContact}/(S_{TimeMakingContact}+E_{TimeMakingContact})) × 100. The cages and chambers were wiped clean with 5% ethanol after each mouse to avoid olfactory cues.

Magnetic cell sorting of microglial cells

The mice were deeply anesthetized with pentobarbital (Narcoren, Merial, Hallbergmoos, Germany) and perfused with ice-cold phosphate-buffered saline. After decapitation, the head was removed and the brain retrieved and put immediately in ice-cold Hank's balanced salt solution (GIBCO, Invitrogen, Karlsruhe, Germany). Tissue dissection and microglial isolation were performed as described elsewhere.³⁶ Briefly, the pelleted single-cell suspension of the hippocampi was resuspended in ice-cold percoll solution composed of myelin gradient buffer plus percoll (GE Healthcare, Little Chalfont, Buckinghamshire, UK) and a layer of phosphate-buffered saline was carefully applied on top. The tubes were then centrifuged at 950 g, 4 °C low brake and acceleration. The cells were resuspended and washed in ice-cold magnetic cell sorting (MACS) buffer (phosphate-buffered saline, 0.5% bovine serum albumin, 2 mm EDTA) followed by incubation with anti-mouse CD11b magnetic beads (Miltenyi Biotech, Bergisch Gladbach, Germany) at 4 °C for 15 min. The cells were resuspended in MACS buffer and passed through medium-sized MACS columns (Miltenyi Biotech) attached to a magnet. The flow-through was discarded and the cells were flushed out of the column in MACS buffer, pulled down by centrifugation and resuspended in 1 ml TRIzol (Thermo Scientific, Schwerte, Germany) snap frozen in liquid nitrogen and stored at −80 °C until library preparation.

mRNA library preparation and sequencing

The messenger RNA (mRNA) libraries were made using TruSeq RNA Sample Preparation Kits v2 setA (#RS-122-2001, Illumina, San Diego, CA, USA) following the manufacturer's instructions. RNA-sequencing (RNA-seq) was performed on mRNA extracted from freshly isolated microglia (as described above) from the hippocampi of control, Poly(I:C) and minocycline-treated Poly(I:C) animals. The Poly(I:C) animals used for the RNA-seq were chosen on the basis of their deficit in PPI compared with control animals (Supplementary Figure 1a). A group of Poly(I:C) animals were treated with minocycline (3 mg kg⁻¹ per day) for 5 weeks and re-tested for PPI to confirm the efficacy of minocycline on this schizophrenic endophenotype. A total amount of six animals per group were used. To obtain a substantial number of microglial cells, hippocampi were pooled together from three animals per group for a final number of two biological replicates per group. The complementary DNA (cDNA) libraries were loaded onto a HiSeq Rapid PE Flow Cell (Illumina) and mRNA-seq libraries were sequenced paired-end (2 × 100 bp) using a HiSeq 2500 sequencer (Illumina).

Quantitative PCR

For quantitative PCR validation of selected genes, we used the RNA extracted for the sequencing experiment. First-strand cDNA synthesis was done with the SuperScript II reverse transcriptase (Invitrogen, Carlsbad, CA, USA) using oligo-dT primers₁₂_–₁₈ (Invitrogen) according to the manufacturer’s instructions. Quantitative real-time PCR reactions were performed in a 7500 Fast Real-Time thermocycler (Applied Biosystems, Carlsbad, CA, USA) using the SYBR Select Master Mix (Applied Biosystems) according to the manufacturer’s instructions. cDNA input ranged between 1 and 5 ng μl⁻¹ of total RNA transcribed into cDNA. The expression data were normalized to beta-actin. The primers used are listed in Supplementary Material 1.

Computational analysis

Paired-end RNA-Seq reads were mapped to the mouse reference genome GCRM38/mm10 with STAR,³⁷ using the following parameters ‘—outFilterMultimapNmax 20 —alignSJoverhangMin 8 —alignSJDBoverhangMin 1 –outfilterMismatchNmax 999 —outFilterMismatchNovelLmax 0.04 —alignIntronMin 20 —alignIntronMax 10^6 —alignMatesGapMax 10^6’. Mapped read pairs were assigned to genes with feature Counts.³⁸ We used gencode version M6 (Ensembl 81) mouse genome annotation. Alternative scaffolds, fix- and novel- patches were removed from the analysis. To identify differentially expressed genes between different conditions, we used R/Bioconductor DeSeq2.³⁹ The analysis was carried out with DeSeq2 default parameters, using raw read counts. Gene ontology (GO) analysis was performed with topGO gene ontology tool, using the ‘elim’ algorithm (nodes=5; Alexa A and Rahnenfuhrer J 2016, topGO: Enrichment Analysis for Gene Ontology, R package version 2.24.0.).

For the comparison of differential expression with APP mouse model, we downloaded the log2 fold changes and the adjusted P-values from Holtman et al.⁴⁰ This (microarray) data set comprised 9938 genes of which 9813 could be matched with our Gencode M6 gene IDs. Among these, 1595 were differentially expressed in the APP model (using adjusted P-value < 0.01). From 1398 differentially expressed genes between Poly and control mice, 736 were present in the matched Holtman et al.⁴⁰ data set and 248 of these were also differentially expressed in the APP model (again using adjusted P-value <0.01). The corresponding Fisher’s exact yields a P-value of 3.8e⁻³⁴ and thus confirms a strong overlap of differentially regulated processes between the two models. PU.1 target genes were retrieved from Satoh et al.⁴¹ As a background control, we selected the same amount of genes that are not targeted by PU.1 but have a similar expression distribution in control samples.

Data accessibility

Deep sequencing data are deposited on Sequence Read Archive (BioProject: PRJNA341344 accession ids: SRR4244948, SRR4244949, SRR4244950, SRR4244951, SRR4244952, SRR4244953) and will be released on publication.

Immunohistochemistry and confocal microscopy

The detailed staining procedure was described elsewhere.²³ For staining of the ionized calcium-binding adaptor molecule 1 (Iba1) and CD18, free-floating 40 μm thick sections were incubated in 10% donkey serum and 0.13% Triton-X in tris-buffered saline solution (TBSplus). The primary antibodies were prepared in TBSplus at the following dilutions: rabbit anti-Iba1 (Wako chemicals, Neuss, Germany, product code: 019-19741) 1:400; rat anti-CD18 (Abcam, Cambridge, UK, ab119830) 1:350. The sections were incubated with the primary antibodies overnight at 4 °C. The secondary antibodies were also prepared in TBSplus at the following dilutions: donkey anti-rabbit Cy3 (Dianova, Hamburg, Germany) 1:350; donkey anti-rat Alexa Fluor 488 (Dianova). Nuclei were stained using DAPI (4',6-diamidino-2-phenylindole), 1:500. The sections were incubated with secondary antibodies at room temperature for 2 h. Confocal Z-stacks of 20 μm of thickness were taken with a Leica SPE confocal microscope (Leica, Bensheim, Germany) through the dentate gyrus of the hippocampus at 20-times magnification. Representative pictures were taken in the proximity of the dentate gyrus at 20-times magnification. The stacks were transformed into one picture using a Z-projection with maximum intensity using ImageJ software.⁴² Mean fluorescence intensity per area for each target (Iba1, CD18, DAPI) throughout the dentate gyrus was measured by ImageJ and divided by the mean fluorescent intensity per area measured for DAPI and averaged for all animals within each group. A total of two distinct hippocampal slices per animal were sampled for the analysis. The images were sampled and analyzed by a person blind to the group identity.

Enzyme-linked immunosorbent assay

Whole protein homogenates, from the hippocampi were obtained by mechanical tissue disruption using a syringe in ice-cold phosphate-buffered saline containing protease inhibitors (complete Ultra Tablet, Roche, Basel, Switzerland). Protein concentration was evaluated by NanoDrop 8000 spectrophotometer (Thermo Scientific, Waltham, MA, USA). Complement component 4a (C4a) levels in hippocampal lysates were measured using the mouse C4a enzyme-linked immunosorbent assay (ELISA) kit (Elabscience Biotechnology, Wuhan, China), according to the manufacturer’s manual. Detection of the cytokines was performed by using the Multiplex Immunoassay ProcartaPlex (Affymetrix eBioscence, Vienna, Austria). This beads-based ELISA allows the detection of the concentration of up to 20 analytes using the Luminex xMAP (multi-analyte profiling) technology. Specifically, the cytokines pattern included tumor necrosis factor-α and interleukin-6 (IL-6; mouse High-Sensitive Assay) combined with IL-1β (Mouse Assay).

Flow cytometry-based phagocytosis assay

Microglia were isolated from the hippocampi and purified via MACS as described above. After isolation, they were resuspended in Dulbecco's Modified Eagle Medium (GIBCO, Invitrogen) supplemented with 10% fetal calf serum, 2 mm l-glutamine, 37 °C. The cells were then seeded onto primary cultured neonatal astrocytes overnight. Fluoresbrite Carboxylate Micropheres (BrightBlue, 4.5 μm) were coated with 5% fetal calf serum for 30 min at room temperature, 1000 r.p.m. The beads were resuspended in Hank's balanced salt solution at a final concentration of 2 × 10⁶ beads per ml. Microglia-astrocyte co-cultures were washed once with Hank's balanced salt solution before 1 ml bead solution was applied. The cells were incubated with the beads for 30 min at 37 °C. Afterwards, they were collected and washed in fluorescence-activated cell sorting buffer. The cells were stained with: CD11b, 1:50 (eBioscience, San Diego, CA, USA) for 15 min at 4 °C. After staining, the cells were washed in fluorescence-activated cell sorting buffer and pulled down at 500 g for 5 min. The cells were resuspended in a propidium iodide solution (1:200 in fluorescence-activated cell sorting buffer) to stain dead cells. The stained cells were transferred to a BD LSRFortessa Flow cytometer (BD Bioscience, Heidelberg, Germany). The phagocytic index as shown in Figure 2c implements the total number of cells that phagocytosed and the number of beads taken up by a given cell. The data were analyzed using FlowJo v10 software (Tree Star, Ashland, OR, USA).

In vitro autoradiography

For the synthesis of [¹⁸F]GE180, [¹⁸F]fluoride was produced at a Scanditronix MC35 cyclotron via the nuclear reaction ¹⁸O(p,n)¹⁸F by proton bombardment of a ¹⁸O-water target, transferred to a shielded hotcell by a stream of argon and trapped on a QMA SepPak SPE cartridge (Waters, Eschborn, Germany, preconditioned with potassium bicarbonate). The [¹⁸F]fluoride was eluted by Kryptofix 2.2.2 (4 mg, 10 μmol) and potassium bicarbonate (1 mg, 10 μmol) in a mixture of water (0.5 ml) and acetonitrile (0.5 ml) into a sealed reaction vessel. This reaction mixture was dried under a stream of argon at 100 °C. Subsequently, without prior cooling of the mixture, the mesylate precursor (GE Healthcare; 0.5–1.0 mg) dissolved in 1 ml acetonitrile (absolute) was added, followed by 10 min of heating at 100 °C. After brief cooling, the mixture was diluted by 3.5 ml water and injected into the high-performance liquid chromatography system (Hichrom ACE 5 C18 columns, 5 μm, 100 × 10 mm) for purification. The product fraction was diluted with water (20 ml) and passed over a tC18 SepPak SPE cartridge (Waters, preconditioned with ethanol and water). After washing the cartridge with water (3 ml), the product was eluted with ethanol (0.5–1.0 ml) and formulated by further dilution with saline.

Sagittal brain sections (16 μm thick) from mice in control, Poly(I:C) and minocycline-treated Poly(I:C) animals were obtained by cryosectioning, mounted on microscopic glass slides and stored at −80 °C until the day of the experiment. The frozen brain sections were dried at room temperature in a stream of cold air, preincubated with 50 mm Tris-HCl, pH 7.4/21 °C at room temperature for 15 min and dried again before incubation with [¹⁸F]GE180 (0.17 MBq ml⁻¹ 50 mm Tris-HCl, pH 7.4/21 °C, containing 120 mm NaCl, 5 mm KCl, 2 mm MgCl₂, 1 mm CaCl₂; 2.5 nm at the start of the incubation) at room temperature for 60 min. Nonspecific binding of [¹⁸F]GE180 was determined by co-incubation with both 20 μm GE180 and 20 μm PK11195. After incubation, the sections were washed two times for 2 min with ice-cold 50 mm Tris-HCl, pH 7.4/4 °C and rinsed in ice-cold distilled water to remove the buffer salts. The slides were dried in a stream of cold air at room temperature and exposed to ¹⁸F-sensitive imaging plates (BAS 2025, Fujifilm, Tokyo, Japan). The imaging plates were scanned in a CR 35 Bio (Dürr NDT, Bietigheim-Bissingen, Germany) scanner with a pixel size of 12.5 μm × 12.5 μm. Binding of [¹⁸F]GE180 was quantified by measuring the optical density in hand-drawn region of interest (the hippocampus) on the respective autoradiogram. The images were analyzed using Aida Image Analyzer v. 4.27 (raytest, Elysia Germany, Straubenhardt, Germany). Specific binding was calculated as total binding minus nonspecific binding for the same region in the adjacent sections. The analysis of the radioligand-binding potential was performed by a person blind to the experimental groups.

Statistical analysis

To assess statistical differences, we used Prism 5 for Windows (Graphpad Software, La Jolla, CA, USA). For the PPI tests, we performed a two-way repeated-measures analysis of variance (ANOVA) to test for the effect of phenotype (Poly(I:C)) and treatment (minocycline) across the whole session followed by Bonferroni post hoc test. For the analysis of the remaining behavioral tests, the phagocytosis assay, the radioligand-binding assay, ELISA and immunohistochemically data a one-way ANOVA (when the data showed a normal distribution) followed by either Bonferroni post hoc test or Newman–Keuls post hoc test were performed. The variance between the groups was estimated through Bartlett's test for equal variances whenever the sample size was equal or bigger than 7. For smaller sample size, estimation was given based on the number of animals and standard deviation.

Results

Minocycline treatment rescues correlates of positive, negative and cognitive symptoms in adult Poly(I:C) animals

In the first set of experiments, we explored the effect of a chronic minocycline treatment on mouse behavioral traits similar to positive, negative and cognitive symptoms displayed by human schizophrenic patients. We used 16 Poly(I:C) animals and 14 controls for behavioral testing. Sensorimotor gating can be measured via the PPI of the startle reflex test in both humans and rodents.^{14, 43} Out of the 16 Poly(I:C) mice, 7 showed a robust deficit in sensorimotor gating (at 69, 73 and 81 dB prepulses; two-way ANOVA, F(2,66)=48.44, P<0.0001, Bonferroni's post hoc test, Poly versus controls, P<0.0001 for all three prepulses, Figures 1b and c). After 5 weeks of minocycline treatment (3 mg kg⁻¹ per day), the same mice displayed a significant rescue in sensorimotor gating (n=7 Poly(I:C) mice before and after minocycline treatment and 14 controls, Bonferroni post hoc test Poly/Mino versus Poly, P<0.001 for the response to 69 dB prepulse, P<0.0001 for the response to 73 dB prepulse and P<0.001 for the response to 81 dB prepulse, Figures 1b and c). There was no difference in the response to the pulse of 120 dB alone across all tested groups and cohorts (Supplementary Figure 2). For the cognitive symptoms domain, we used the novel object recognition test for working memory.⁴⁴ Poly(I:C) mice displayed working memory deficits in the novel object recognition test (Figures 1d and e), where their percentage of exploration of the new object over the total exploration of the objects (42%±8.8) was significantly lower compared with controls (81%±4.3). Minocycline treatment significantly improved their ability to discriminate a novel object from a familiar one (69%±4.2, n=16 Poly(I:C) mice before and after minocycline treatment and 14 controls, one-way ANOVA, F(2,36)=10.2, P=0.0003, Bonferroni post hoc test Poly versus control P<0.0001 and Poly versus Poly/Mino P<0.05). For readout of a negative-like symptom, we assessed social behavior. Poly(I:C) mice displayed deficits in social behavior (Figures 1f and g), shown as a significantly decreased percentage of time making contact with a stranger mouse (58%±3.8) as compared with controls (72%±3.8). After minocycline treatment, the percentage of time the mice spent with the stranger mouse normalized to control levels (70%±2.1, n=16 Poly(I:C) mice before and after minocycline treatment and 14 controls, one-way ANOVA, F(2,43)=7.41, P=0.0017, Bonferroni post hoc test Poly versus control P<0.05 and Poly versus Poly/Mino P<0.001). These results show similarities to the findings in human clinical trials of minocycline treatment alleviating negative, positive and cognitive symptoms in patients with schizophrenia.^{16, 17, 18, 20, 21} As deficits in rodent PPI reliably suggest a behavioral phenotype associated with schizophrenia in Poly(I:C) mice, we used this test to select only mice with a PPI deficit for all further experiments within this study. Supplementary Figures 1a–d show the final selection of the Poly(I:C) cohorts after minocycline treatment used for every experiment performed in this study. Supplementary Figures 3a–e show the single data points of the percentage of PPI for the initial selection for Poly(I:C) versus control mice before minocycline treatment.

Hippocampal microglial cells from Poly(I:C) mice show an altered transcriptome signature that is rescued by minocycline treatment

To obtain a comprehensive overview of the pathways involved in the microglial phenotype associated with maternal immune activation during pregnancy, we performed a mRNA-seq of freshly isolated microglia from the hippocampi of adult male Poly(I:C) mice, Poly(I:C) mice treated with minocycline and control animals. A total of six animals per group were used. To obtain a substantial number of microglial cells, hippocampi were pooled together from three animals per group for a final number of two biological replicates per group. We selected Poly(I:C) animals with a significant deficit in PPI (Supplementary Figures 1a and 3b). In total, we obtained between 19.8 and 26.9 million paired-end reads per sample with 83%–91% concordant pair alignment rate (see the 'Materials and methods' section). The comparison of the replicates demonstrated a high reproducibility of the data (Supplementary Figure 4a). Notably, further comparison of the samples showed that the mRNA expression profile of the two minocycline-treated Poly(I:C) samples were similar to the control samples, while the profile of both Poly(I:C) samples were distant from the latter groups (Supplementary Figure 4b). Differential expression analysis revealed that microglia derived from the hippocampi of adult Poly(I:C) mice presents with wide-scale changes in transcriptome relative to control. We identified 1398 significantly deregulated genes (adjusted P-value cutoff <0.01), of which 402 were upregulated and 563 downregulated more than two fold. When comparing hippocampal microglia from Poly(I:C) mice treated with minocycline and untreated Poly(I:C) using the same cutoff as above, we found 943 deregulated genes of which 441 were upregulated and 193 downregulated more than twofold. However, we found only 21 genes to be significantly deregulated, when comparing microglial cells from minocycline-treated Poly(I:C) mice and controls already showing that minocycline normalized the Poly(I:C)-associated microglia phenotype towards the naive microglial phenotype (Figure 3a, Supplementary Table 1). To identify the deregulated biological processes in Poly(I:C) mice, we separately subjected the up- (cluster 2) and downregulated (cluster 1) genes to Gene Ontology analysis.

In cluster 1, we found significant enrichment of genes associated with processes such as inflammatory response, cell migration and phagocytosis (Figure 3a; for a complete overview of the gene ontology analysis, see Supplementary Table 2a). Of particular interest are the downregulated genes that encode for cell surface receptors associated with ‘pro-phagocytic’ signals that initiate and regulate phagocytosis. These genes include Fcgr1, Itgav and P2ry6 (Figure 3a, Supplementary Table 1). Interestingly, genes also encoding microglial surface receptors that sense neuronal ‘anti-phagocytic’ signals, including Sirpa, Siglece and Cx3cr1 were downregulated (Figure 3a, Supplementary Table 1). In cluster 2, we found significant enrichment of genes associated with extracellular matrix organization, embryonic development, positive regulation of long-term neuronal plasticity and angiogenesis (Figure 3a, Supplementary Table 2a).

In cluster 1, we detected a significant deregulation of Spi1, Irf8 and Jun (Supplementary Table 1) that we validated via quantitative PCR comparing their relative expression to transcripts per million values obtained from the mRNA-seq (Supplementary Figures 4c and d). Importantly, Spi1 and Irf8 transcription factors may act as master switches responsible for such a drastic change in the microglial phenotype as they are crucial for microglial development and maintenance.^{41, 45, 46} Adult minocycline treatment restored the mRNA levels of these two transcription factors. In particular, Spi1, which encodes the transcription factor PU.1, may control around 5000 genes in microglia.⁴¹ We therefore compared the differential expression of previously published PU.1 target genes⁴¹ to expression-matched controls. This comparison demonstrated that PU.1 target genes were significantly downregulated in Poly(I:C) mice and restored on minocycline treatment (Figure 3b). We therefore hypothesize that the drastic changes in microglial transcriptome may arise from the downregulation of PU.1 expression along with its target genes.

Increased pro-inflammatory signaling in Poly(I:C) hippocampal microglia

Studies conducted so far in vivo and in postmortem human brains and in the Poly(I:C) model of schizophrenia reported upregulation of classical markers of microglial activation such as increased binding of radioligands to the TSPO and increased cytokine levels (reviewed in refs 14, 47). As our transcriptome analysis reveals a marked general downregulation of genes involved in processes related to immune cell activation, we analyzed whether these markers are altered and whether minocycline prevents this alteration.

To determine region-specific TSPO binding, we performed an autoradiographic study of hippocampal slices using the TSPO ligand [¹⁸F]GE180.⁴⁸ We used a strategy similar to the one adopted by Kreisl et al.⁴⁹ where the authors showed that schizophrenic patients display an increased binding potential to the TSPO in postmortem brain tissue relative to controls. These findings are similar to the in vivo results utilizing positron emission tomography scans.^{50, 51, 52} We selected male Poly(I:C) mice with PPI deficits and randomly assigned a group of these mice to minocycline treatment and investigated the radioligand [¹⁸F]GE180 binding capacity (Supplementary Figures 1b and 3c). In Figure 4a, we show representative picture of a slice from a control animal, used for the analysis of binding capacity along with a slice labeled with Nissl stain to outline anatomical structures (Figure 4b). Poly(I:C) mice have an increased binding potential expressed as relative intensity of the signal detected from the bound radioligand in the hippocampus as compared with control animals (Poly, n=5, 808±63.4 versus controls, n=6, 399±87, Figure 4c). Minocycline treatment normalized the radioligand [¹⁸F]GE180 binding potential in the hippocampus towards control levels (Poly/Mino, n=6, 280±35.7, Figure 4c, one-way ANOVA F(14,16)=5.64, P=0.016; Bonferroni's post hoc test Poly versus controls and Poly/Mino versus Poly, P<0.05).

As a second indicator of activation, we determined the immunoreactivity of Iba1.⁵³ We found that Poly(I:C) animals displayed a higher Iba1 immunoreactivity expressed as relative intensity (11±2, n=7) as compared with controls (4.8±1, n=5), while minocycline-treated mice showed an Iba1 immunoreactivity comparable to controls (3.1±0.6, n=4, Figures 4d and e, one-way ANOVA, F(2,13)=6.41, P=0.01, Newman–Keuls post hoc test Poly versus controls and Poly versus Poly/Mino, P<0.05). Next, we measured by means of ELISA the levels (pg/ng) of the following cytokines in whole hippocampal tissue from control (n=5), Poly(I:C) (n=5) and Poly(I:C)/Mino (n=4) mice: IL-1β, IL-6 and tumor necrosis factor-α (Figures 4f–h). We only observed a significant increase in IL-6 in Poly(I:C) hippocampal homogenates (0.25±0.022) as compared with controls (0.14±0.016) that was abolished in the Poly(I:C)/Mino group (0.16±0.031, Figure 4h, one-way ANOVA F(2,11)=6.62, P=0.013, Newman–Keuls post hoc test Poly versus controls and Poly versus Poly/Mino, P<0.05). The levels of IL-1β and tumor necrosis factor-α did not change between groups (Figures 4f and g). For the PPI measurement of this cohort, see Supplementary Figures 1d and 3e.

Decreased phagocytic activity displayed by Poly(I:C) hippocampal microglia is rescued by chronic minocycline treatment

Our transcriptome analysis indicated that genes involved in phagocytosis such as Spi1, Syk, Hck, Dock2 and Elmo1 are downregulated (Supplementary Table 1). To determine phagocytic activity on a functional level, we studied the incorporation of fluorescent latex beads within 30 min and quantified it by fluorescence-activated cell sorting analysis. Figure 2a shows the gating strategy to determine the uptake of the beads. In Figure 2b, we show representative histograms from all three experimental groups. The peaks represent the number of microglia that phagocytosed one, two or three beads. A detailed gating strategy is presented in Supplementary Figure 5. The phagocytosis assay conducted on hippocampal microglia revealed that these cells have a decreased phagocytic index in Poly(I:C) animals (0.58±0.06, n=12) as compared with controls (1.0±0.08, n=17, Figure 2c). Microglial cells isolated from hippocampi of Poly(I:C) mice treated with minocycline showed normal phagocytosis activity, comparable to control animals and significantly higher as compared with untreated Poly(I:C) mice (0.91±0.04, n=11, Figure 2c, one-way ANOVA, F(2,37)=10.21, P=0.003, Bonferroni post hoc test controls versus Poly, P<0.0001, Poly versus Poly/Mino, P<0.001). For the PPI measurements of this cohort, see Supplementary Figures 1c and 3d.

The phenotype of microglia derived from the MIA model is comparable to the phenotype of microglia derived from the APP/PS1 Alzheimer’s model

We previously demonstrated that microglia have decreased phagocytic functions in two mouse models of Alzheimer’s disease including the APP/PS1 mouse.⁵⁴ Moreover, Krstic et al.²⁵ showed that the offspring of Poly(I:C)-challenged dams are prone to develop an Alzheimer’s-like phenotype with aging, including increased levels of amyloid precursor protein and its proteolytic fragments, as well as mislocalization and hyperphosphorylation of tau in somatodendritic compartments. To test whether the transcriptome of microglia derived from Poly(I:C) mice display any similarity to the transcriptional program attained by microglia from the APP/PS1 mice, we compared the differentially expressed genes in microglia from the Poly(I:C) mice versus control with the differentially expressed genes in microglia from the APP/PS1 mice versus controls (published previously⁴⁰). We detected a high correlation between the expression changes in APP/PS1 and Poly(I:C) mice compared with controls (Pearson’s r=0.59, Figure 2d). We identified 248 common deregulated genes in Poly(I:C) and APP/PS1 microglia (Figure 2e). On gene ontology analysis, we found a significant enrichment in genes associated with phagocytosis that confirms impairment of this particular function in both animal models; similar results were obtained in the functional phagocytosis assay (Supplementary Table 2b). For a better view and understanding of the deregulated genes pertaining to different phagocytic functions, we show a schematic representation of some phagocytosis-associated pathways in Figure 2f.

Changes in the complement system in the hippocampus of Poly(I:C) mice

The complement system consists of components crucial for proper microglial-mediated phagocytosis of synapses especially during postnatal brain development.⁵⁵ In a recent study, Sekar et al.⁵⁶ demonstrated that individual variations in the allele coding for the complement component 4 (C4) can confer high genetic risk for schizophrenia. Microglia expresses the receptor for C4, which is composed of both CD18 and CD11c.⁵⁷ Accordingly, we measured C4a levels via ELISA on whole hippocampal homogenates and the expression of CD18 by Iba1-positive cells by fluorescent immunohistochemistry to see whether they are altered in hippocampal tissue of Poly(I:C) mice as compared with controls. We found that C4a levels (pg/ng) were unaltered in hippocampal tissue from Poly(I:C) mice (27.1±2.4, n=5) as compared with controls (26.4±1.8, n=5) and Poly(I:C) mice treated with minocycline (24.1±3.5, n=4, Figure 5a). On the other hand, the immunoreactivity for CD18 was significantly increased in Iba1-positive microglia residing in the dentate gyrus of the hippocampus of Poly(I:C) mice (0.24±0.059, n=7, Figures 5b and c) as compared with controls (0.086±0.019, n=5), whereas minocycline-treated Poly(I:C) animals displayed immunoreactivity comparable to baseline level (0.057±0.014, n=4, Figures 5b and c, one-way ANOVA F(2,13)=4.92, P=0.026, Newman–Keuls post hoc test Poly versus controls and Poly versus Poly/Mino, P<0.05).

Discussion

We here demonstrate that a one-time MIA with a viral mimic during pregnancy leads to a drastic change in transcriptome and phagocytic activity of microglial cells in the hippocampus of the adult offspring. These changes were associated with schizophrenic-like behavioral abnormalities in Poly(I:C) mice. Impaired sensory-motor gating, social interaction and memory recollection in our study concur with similar findings in previous studies (reviewed in refs 14, 58). We further provide evidence of how minocycline reverses the microglial alterations on the functional and transcriptional level. Importantly, our study revealed that behavioral deficits linked with schizophrenia and changes in microglia caused by a prenatal immune challenge can be alleviated by adult minocycline treatment, indicating that the developmental changes are not irreversible. This opens the opportunity to target microglia cells in the adult as a novel therapeutic approach for schizophrenic patients. Future research should address the question as to whether the beneficial effects of minocycline on the behavioral, cellular and molecular level are long-lasting once the treatment is stopped, especially regarding microglial transcriptome signature.

Minocycline readily crosses the blood–brain barrier⁵⁹ and is already successfully used in clinical trials and impacts human clinical data.⁶⁰ Several studies have shown its complex effects on microglia and macrophages, which depend on the particular physiological stimulus these cells are facing.^{61, 62, 63} Although we found that minocycline reversed the MIA-induced phenotype of microglia, we are aware that it is not acting only on microglia. Its neuroprotective action along with its influence on oligodendrocytes and astrocytes may participate together with its action on microglial cells to the outcome of minocycline treatment (reviewed in ref. 64). It is also feasible that antibiotic actions of minocycline may influence microglial immune function in the central nervous system indirectly via the microbiome, which has been shown to modulate microglial cell physiology.⁶⁵ The present study did not address minocycline effects on control animals. It would be relevant for future studies to screen the transcriptome signature of microglia from control animals treated with minocycline. However, in our previous study, when minocycline was administered to both Poly(I:C) animals and control animals, we did not observe any change of the behavioral, cellular or molecular measures tested.²³

In the present study, hippocampal microglia from male Poly(I:C) mice displayed downregulation in expression of genes associated with cell activation, immune response, motility and phagocytosis. Of particular interest are the downregulated genes that encode cell surface receptors associated with ‘pro-phagocytosis’/‘anti-phagocytosis’ signals (Fcgr1, Itgav, P2ry6, Sirpa, Siglece, Cx3cr1). These receptors constitute the ‘sensome’ by which microglia and neurons communicate and they control the phagocytosis of neurons or synapses by microglia.⁶⁶ Furthermore, genes such as Syk, Mertk, Hck, Dock1 and Elmo1 which encode proteins controlling cytoskeletal rearrangement and phagocytosis⁶⁷ were also found to be downregulated in Poly(I:C) animals. Importantly, two transcription factors that are crucial for microglial cell identity, phenotype and microglia-specific functions, namely PU.1 (encoded by the gene Sfpi1) and interferon regulatory factor 8 (Irf8) were downregulated in our study.^{41, 46, 68} It was shown that silencing of PU.1 impairs phagocytosis in human microglial cultures.⁴⁶ Comparing our data set with a recent Chip-seq study for PU.1,⁴¹ we found a major shift of the genes that are co-precipitated with PU.1 in Poly(I:C)-derived hippocampal microglia. Our ex vivo phagocytosis assay validated the fact that the phagocytic activity of hippocampal microglial cells in Poly(I:C) offspring is significantly decreased. We hypothesize that the disrupted sensome and phagocytic activity in hippocampal microglial cells participates in the behavioral dysfunctions observed in these mice.

The downregulation of phagocytosis is contrasted by increased expression of indicators of microglial activation, such as TSPO binding, Iba1 immunoreactivity and IL-6 levels. TSPO binding is not limited but mainly attributed to microglia in the current literature. Our finding is in line with the increasing evidence of a higher binding potential to TSPO in the brains of human schizophrenic patients^{50, 51, 52} especially in the hippocampus.⁵¹ However, in general, a direct link between changes in TSPO and microglial activation should be addressed with care. Studies have indeed demonstrated changes in microglial TSPO expression and binding potential in pathological conditions. Nevertheless, TSPO is expressed also by other brain cells such as ependymal and endothelial cells.⁶⁹ In addition, we observed a stronger radioligand binding in certain brain regions such as the brain stem and olfactory bulbs (see Figure 4a). This is likely due to the fact that TSPO is a mitochondrial protein responsible for the translocation of cholesterol and, therefore, it may show different expression patterns according to the specific metabolic needs of a particular brain region at any given moment.

It is important to emphasize that CD18 and Iba1 were significantly downregulated at the mRNA level, but showed an increased immunoreactivity at the protein level. Divergent mRNA and protein kinetics may depend on the relative half-life of a given mRNA versus its protein, which may not correlate.⁷⁰ Our data show that the kinetic of protein levels might not correspond with the kinetic of mRNA levels, which might also contribute to the controversial findings between studies.

Previous studies utilizing the maternal immune activation model including ours focused either on microglia density or cytokine production.^{23, 71, 72, 73, 74} Although some studies including the evaluation of human postmortem tissue reported increased microglia density in defined brain areas, others did not find major changes. Similar controversial data exist for cytokine levels in the brain of patients with schizophrenia as well as in the present animal model (reviewed in ref. 3). These differences could be attributed to microglia heterogeneity with respect to region, gender or age. One recent study highlighted the importance of microglia temporal heterogeneity during development.⁷⁵ A number of studies showed that microglia attain different transcriptional programs in distinct brain regions^{76, 77, 78} or differ between male and female brains.⁷⁹ Thus, when comparing data, it is important to consider the developmental time point, the brain region and gender as these factors might attribute to the reported differences between the above-mentioned studies.

Microglial cell polarization is a complex matter, as it has been demonstrated in Alzheimer’s disease where microglia can co-express classical markers of activation concomitant with markers of opposite, non-classically activated phenotypes.⁸⁰ This is in line with the microglia phenotype we describe in the current study: an increase in IL-6, TSPO binding and Iba1 reactivity is accompanied by unchanged levels of tumor necrosis factor-α and IL-1β and impairment in phagocytosis. We have previously reported impairment in phagocytosis of microglia associated with plaques in the APP/PS1 model for Alzheimer’s disease using a bead-based phagocytosis assay similar what was used in the current study.⁵⁴ It was also shown that immunotherapeutic amyloid clearance was less effective and microglia response altered when phagocytosis was impaired.^{81, 82} Thus, downregulation of functional microglial phagocytosis is a common feature in Alzheimer’s disease and MIA models. When comparing the microglial transcriptome changes in our model (Poly(I:C) versus control) with the changes observed in the APP/PS1 mice (APP/PS1 versus control),⁴⁰ we see a strong overlap in genes of the phagocytosis pathway. Vav1 and Elmo have been shown to be crucial for fibrillary β-amyloid-stimulated oxidative burst and phagocytosis.⁸³ Considering this overlap, especially in terms of phagocytic functions and the complex phenotype attained by Poly(I:C) microglia, our data support the hypothesis that early immune challenge can lead to an Alzheimer’s-like pathology as proposed by Krstic and colleagues.^{25, 26} Data-driven analyses of brain structural variation by magnetic resonance imaging revealed a network of brain regions that spatially recapitulates the pattern of brain abnormalities observed in both schizophrenia and Alzheimer’s disease. The authors of this study suggest that the common spatial pattern of abnormalities observed in these two disorders might be influenced by the timing of their separate and distinct pathological processes in disrupting cerebral development and aging, respectively.⁸⁴

With respect to the complement-linked phagocytic activity, it has been shown that complement component C3, complement receptor type 3 and C1q contribute to the early and excessive synaptic loss through microglia-mediated phagocytosis in a model of Alzheimer’s disease.⁸⁵ In a recent study, Sekar et al.⁵⁶ found an association between schizophrenia and variations in the major histocompatibility complex locus on alleles affecting the expression of the complement component 4 (C4) particularly in the hippocampus of schizophrenic patients.⁵⁶ The main function of C4 is to activate the complement component 3 (C3) for proper synaptic elimination during development, and the authors suggest this as a mechanism by which schizophrenic individuals exhibit decreased number of synapses in diverse brain regions in postmortem studies.⁵⁶ In our mouse model, we did not detect changes of C4a protein in the hippocampal homogenates between the groups, which is not surprising as the MIA mice do not harbor a genetic C4 mutation like the human subjects. However, changes in CD18 (part of the receptor for C3 and C4) indicates that prenatal insult perturbs the complement system also in the absence of genetic background, possibly participating to the development of a defect brain micro-circuitry.

We propose that an immune challenge during prenatal development causes changes in microglial function in adulthood that shares common features with changes in microglial function in neurodegenerative diseases such as Alzheimer’s disease. Early-life events such as MIA might contribute to the neurogenic reserve by setting the basis on how an individual might withstand secondary challenges later in life.⁸⁶ Lahiri et al.²⁸ propose a 'Latent Early-life Associated Regulation' model, positing latent changes in the expression of specific genes initially primed at the developmental stage of life. In this model, environmental agents epigenetically disturb gene regulation in a long-term manner, beginning at early developmental stages, but these perturbations might not have pathological results until significantly later in life.²⁸ Our data provide evidence for this hypothesis on the level of microglia functionality. By identifying common denominators and traits in microglia derived from mouse models of neurodegenerative and psychiatric disorders, we here highlight the importance of the brain’s immune system as a target for add-on therapeutic interventions, already starting before the onset of symptoms.

References

Elsayed M, Magistretti PJ . A new outlook on mental illnesses: glial involvement beyond the glue. Front Cell Neurosci 2015; 9: 468.
Article PubMed PubMed Central Google Scholar
Laskaris LE, Di Biase MA, Everall I, Chana G, Christopoulos A, Skafidas E et al. Microglial activation and progressive brain changes in schizophrenia. Br J Pharmacol 2016; 173: 666–680.
Article CAS PubMed PubMed Central Google Scholar
Trepanier MO, Hopperton KE, Mizrahi R, Mechawar N, Bazinet RP . Postmortem evidence of cerebral inflammation in schizophrenia: a systematic review. Mol Psychiatry 2016; 21: 1009–1026.
Article CAS PubMed PubMed Central Google Scholar
Bayer TA, Buslei R, Havas L, Falkai P . Evidence for activation of microglia in patients with psychiatric illnesses. Neurosci Lett 1999; 271: 126–128.
Article CAS PubMed Google Scholar
Salter MW, Beggs S . Sublime microglia: expanding roles for the guardians of the CNS. Cell 2014; 158: 15–24.
Article CAS PubMed Google Scholar
Cunningham CL, Martínez-Cerdeño V, Noctor SC . Microglia regulate the number of neural precursor cells in the developing cerebral cortex. J Neurosci 2013; 33: 4216–4233.
Article CAS PubMed PubMed Central Google Scholar
Sierra A, Encinas JM, Deudero JJP, Chancey JH, Enikolopov G, Overstreet-Wadiche LS et al. Microglia shape adult hippocampal neurogenesis through apoptosis-coupled phagocytosis. Cell Stem Cell 2010; 7: 483–495.
Article CAS PubMed PubMed Central Google Scholar
Frankle WG, Lerma J, Laruelle M . The synaptic hypothesis of Schizophrenia. Neuron 2003; 39: 205–216.
Article CAS PubMed Google Scholar
Toro CT, Deakin JFW . Adult neurogenesis and schizophrenia: a window on abnormal early brain development? Schizophr Res 2007; 90: 1–14.
Article CAS PubMed Google Scholar
Wolf SA, Melnik A, Kempermann G . Physical exercise increases adult neurogenesis and telomerase activity, and improves behavioral deficits in a mouse model of schizophrenia. Brain Behav Immun 2011; 25: 971–980.
Article CAS PubMed Google Scholar
Glausier JR, Lewis Da . Dendritic spine pathology in schizophrenia. Neuroscience 2013; 251: 90–107.
Article CAS PubMed Google Scholar
Brown AS, Begg MD, Gravenstein S, Schaefer CA, Wyatt RJ, Bresnahan M et al. Serologic evidence of prenatal influenza in the etiology of schizophrenia. Arch Gen Psychiatry 2004; 61: 774–780.
Article PubMed Google Scholar
Cai L, Wan C-L, He L, de Jong S, Chou K-C . Gestational influenza increases the risk of psychosis in adults. Med Chem 2015; 11: 676–682.
Article CAS PubMed Google Scholar
Mattei D, Schweibold R, Wolf SA . Brain in flames - animal models of psychosis: utility and limitations. Neuropsychiatr Dis Treat 2015; 11: 1313–1329.
CAS PubMed PubMed Central Google Scholar
Meyer U, Feldon J, Schedlowski M, Yee BK . Towards an immuno-precipitated neurodevelopmental animal model of schizophrenia. Neurosci Biobehav Rev 2005; 29: 913–947.
Article CAS PubMed Google Scholar
Chaudhry IB, Hallak J, Husain N, Minhas F, Stirling J, Richardson P et al. Minocycline benefits negative symptoms in early schizophrenia: a randomised double-blind placebo-controlled clinical trial in patients on standard treatment. J Psychopharmacol 2012; 26: 1185–1193.
Article PubMed CAS Google Scholar
Chaves C, Marque CR, Maia-de-Oliveira JP, Wichert-Ana L, Ferrari TB, Santos AC et al. Effects of minocycline add-on treatment on brain morphometry and cerebral perfusion in recent-onset schizophrenia. Schizophr Res 2015; 161: 439–445.
Article PubMed Google Scholar
Ghanizadeh A, Dehbozorgi S, OmraniSigaroodi M, Rezaei Z . Minocycline as add-on treatment decreases the negative symptoms of schizophrenia; a randomized placebo-controlled clinical trial. Recent Pat Inflamm Allergy Drug Discov 2014; 8: 211–215.
Article CAS PubMed Google Scholar
Jhamnani K, Shivakumar V, Kalmady S, Rao NP, Venkatasubramanian G . Successful use of add-on minocycline for treatment of persistent negative symptoms in schizophrenia. J Neuropsychiatry Clin Neurosci 2013; 25: E06–E07.
Article PubMed Google Scholar
Kelly DL, Sullivan KM, McEvoy JP, McMahon RP, Wehring HJ, Gold JM et al. Adjunctive minocycline in clozapine-treated schizophrenia patients with persistent symptoms. J Clin Psychopharmacol 2015; 35: 374–381.
CAS PubMed PubMed Central Google Scholar
Levkovitz Y, Mendlovich S, Riwkes S, Braw Y, Levkovitch-Verbin H, Gal G et al. A double-blind, randomized study of minocycline for the treatment of negative and cognitive symptoms in early-phase schizophrenia. J Clin Psychiatry 2010; 71: 138–149.
Article CAS PubMed Google Scholar
Liu F, Guo X, Wu R, Ou J, Zheng Y, Zhang B et al. Minocycline supplementation for treatment of negative symptoms in early-phase schizophrenia: a double blind, randomized, controlled trial. Schizophr Res 2014; 153: 169–176.
Article PubMed Google Scholar
Mattei D, Djodari-Irani A, Hadar R, Pelz A, de Cossío LF, Goetz T et al. Minocycline rescues decrease in neurogenesis, increase in microglia cytokines and deficits in sensorimotor gating in an animal model of schizophrenia. Brain Behav Immun 2014; 38: 175–184.
Article CAS PubMed Google Scholar
Giovanoli S, Engler H, Engler A, Richetto J, Feldon J, Riva MA et al. Preventive effects of minocycline in a neurodevelopmental two-hit model with relevance to schizophrenia. Transl Psychiatry 2016; 6: e772.
Article CAS PubMed PubMed Central Google Scholar
Krstic D, Madhusudan A, Doehner J, Vogel P, Notter T, Imhof C et al. Systemic immune challenges trigger and drive Alzheimer-like neuropathology in mice. J Neuroinflammation 2012; 9: 151.
Article CAS PubMed PubMed Central Google Scholar
Knuesel I, Chicha L, Britschgi M, Schobel SA, Bodmer M, Hellings JA et al. Maternal immune activation and abnormal brain development across CNS disorders. Nat Rev Neurol 2014; 10: 643–660.
Article CAS PubMed Google Scholar
Tartaglione AM, Venerosi A, Calamandrei G . Early-life toxic insults and onset of sporadic neurodegenerative diseases-an overview of experimental studies. Curr Top Behav Neurosci 2016; 29: 231–264.
Article CAS PubMed Google Scholar
Lahiri DK, Maloney B, Zawia NH . The LEARn model: an epigenetic explanation for idiopathic neurobiological diseases. Mol Psychiatry 2009; 14: 992–1003.
Article CAS PubMed PubMed Central Google Scholar
Pajonk F-G, Wobrock T, Gruber O, Scherk H, Berner D, Kaizl I et al. Hippocampal plasticity in response to exercise in schizophrenia. Arch Gen Psychiatry 2010; 67: 133–143.
Article PubMed Google Scholar
Tamminga Ca, Stan AD, Wagner AD . The hippocampal formation in schizophrenia. Am J Psychiatry 2010; 167: 1178–1193.
Article PubMed Google Scholar
Tregellas JR, Davalos DB, Rojas DC, Waldo MC, Gibson L, Wylie K et al. Increased hemodynamic response in the hippocampus, thalamus and prefrontal cortex during abnormal sensory gating in schizophrenia. Schizophr Res 2007; 92: 262–272.
Article PubMed PubMed Central Google Scholar
van Erp TGM, Hibar DP, Rasmussen JM, Glahn DC, Pearlson GD, Andreassen OA et al. Subcortical brain volume abnormalities in 2028 individuals with schizophrenia and 2540 healthy controls via the ENIGMA consortium. Mol Psychiatry 2016; 21: 585.
Article CAS PubMed Google Scholar
Chaves C, Zuardi AW, Hallak JEC . Minocycline as a potential treatment in the early stages of schizophrenia: a translational approach. Ther Targets Neurol Dis 2015; 2: e580.
Google Scholar
Reisinger S, Khan D, Kong E, Berger A, Pollak A, Pollak DD . The poly(I:C)-induced maternal immune activation model in preclinical neuropsychiatric drug discovery. Pharmacol Ther 2015; 149: 213–226.
Article CAS PubMed Google Scholar
Antunes M, Biala G . The novel object recognition memory: neurobiology, test procedure, and its modifications. Cogn Process 2012; 13: 93–110.
Article CAS PubMed Google Scholar
Szulzewsky F, Pelz A, Feng X, Synowitz M, Markovic D, Langmann T et al. Glioma-associated microglia/macrophages display an expression profile different from M1 and M2 polarization and highly express Gpnmb and Spp1. PLoS ONE 2015; 10: e0116644.
Article PubMed PubMed Central CAS Google Scholar
Dobin A, Davis CA, Schlesinger F, Drenkow J, Zaleski C, Jha S et al. STAR: ultrafast universal RNA-seq aligner. Bioinformatics 2013; 29: 15–21.
Article CAS PubMed Google Scholar
Liao Y, Smyth GK, Shi W . featureCounts: an efficient general purpose program for assigning sequence reads to genomic features. Bioinformatics 2014; 30: 923–930.
Article CAS PubMed Google Scholar
Love MI, Huber W, Anders S . Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol 2014; 15: 550.
Article PubMed PubMed Central CAS Google Scholar
Holtman IR, Raj DD, Miller JA, Schaafsma W, Yin Z, Brouwer N et al. Induction of a common microglia gene expression signature by aging and neurodegenerative conditions: a co-expression meta-analysis. Acta Neuropathol Commun 2015; 3: 31.
Article PubMed PubMed Central CAS Google Scholar
Satoh J-I, Asahina N, Kitano S, Kino Y . A comprehensive profile of ChIP-seq-based PU.1/Spi1 target genes in microglia. Gene Regul Syst Biol 2014; 8: 127–139.
CAS Google Scholar
Schneider CA, Rasband WS, Eliceiri KW . NIH image to ImageJ: 25 years of image analysis. Nat Methods 2012; 9: 671–675.
Article CAS PubMed PubMed Central Google Scholar
Braff DL, Geyer MA . Sensorimotor gating and schizophrenia. Human and animal model studies. Arch Gen Psychiatry 1990; 47: 181–188.
Article CAS PubMed Google Scholar
Lesh TA, Niendam TA, Minzenberg MJ, Carter CS . Cognitive control deficits in schizophrenia: mechanisms and meaning. Neuropsychopharmacology 2011; 36: 316–338.
Article PubMed Google Scholar
Horiuchi M, Wakayama K, Itoh A, Kawai K, Pleasure D, Ozato K et al. Interferon regulatory factor 8/interferon consensus sequence binding protein is a critical transcription factor for the physiological phenotype of microglia. J Neuroinflammation 2012; 9: 227.
Article CAS PubMed PubMed Central Google Scholar
Smith AM, Gibbons HM, Oldfield RL, Bergin PM, Mee EW, Faull RLM et al. The transcription factor PU.1 is critical for viability and function of human brain microglia. Glia 2013; 61: 929–942.
Article PubMed Google Scholar
Laskaris LE, Di Biase MA, Everall I, Chana G, Christopoulos A, Skafidas E et al. Microglial activation and progressive brain changes in schizophrenia. Br J Pharmacol 2016; 173: 666–680.
Article CAS PubMed PubMed Central Google Scholar
Airas L, Dickens AM, Elo P, Marjamaki P, Johansson J, Eskola O et al. In vivo PET imaging demonstrates diminished microglial activation after fingolimod treatment in an animal model of multiple sclerosis. J Nucl Med 2015; 56: 305–310.
Article PubMed CAS Google Scholar
Kreisl WC, Jenko KJ, Hines CS, Lyoo CH, Corona W, Morse CL et al. A genetic polymorphism for translocator protein 18 kDa affects both in vitro and in vivo radioligand binding in human brain to this putative biomarker of neuroinflammation. J Cereb Blood Flow Metab 2013; 33: 53–58.
Article CAS PubMed Google Scholar
Bloomfield PS, Selvaraj S, Veronese M, Rizzo G, Bertoldo A, Owen DR et al. Microglial activity in people at ultra high risk of psychosis and in schizophrenia: an [(11)C]PBR28 PET Brain Imaging Study. Am J Psychiatry 2016; 173: 44–52.
Article PubMed Google Scholar
Doorduin J, de Vries EFJ, Willemsen ATM, de Groot JC, Dierckx Ra, Klein HC . Neuroinflammation in schizophrenia-related psychosis: a PET study. J Nucl Med 2009; 50: 1801–1807.
Article PubMed Google Scholar
Kenk M, Selvanathan T, Rao N, Suridjan I, Rusjan P, Remington G et al. Imaging neuroinflammation in gray and white matter in schizophrenia: an in vivo PET study with [18F]-FEPPA. Schizophr Bull 2015; 41: 85–93.
Article PubMed Google Scholar
Hovens I, Nyakas C, Schoemaker R . A novel method for evaluating microglial activation using ionized calcium-binding adaptor protein-1 staining: cell body to cell size ratio. Neuroimmunol Neuroinflammation 2014; 1: 82.
Article Google Scholar
Krabbe G, Halle A, Matyash V, Rinnenthal JL, Eom GD, Bernhardt U et al. Functional impairment of microglia coincides with Beta-amyloid deposition in mice with Alzheimer-like pathology. PLoS ONE 2013; 8: e60921.
Article CAS PubMed PubMed Central Google Scholar
Schafer DP, Lehrman EK, Kautzman AG, Koyama R, Mardinly AR, Yamasaki R et al. Microglia sculpt postnatal neural circuits in an activity and complement-dependent manner. Neuron 2012; 74: 691–705.
Article CAS PubMed PubMed Central Google Scholar
Sekar A, Bialas AR, de Rivera H, Davis A, Hammond TR, Kamitaki N et al. Schizophrenia risk from complex variation of complement component 4. Nature 2016; 530: 177–183.
Article CAS PubMed PubMed Central Google Scholar
Stephan AH, Barres BA, Stevens B . The complement system: an unexpected role in synaptic pruning during development and disease. Annu Rev Neurosci 2012; 35: 369–389.
Article CAS PubMed Google Scholar
Meyer U . Prenatal poly(i:C) exposure and other developmental immune activation models in rodent systems. Biol Psychiatry 2014; 75: 307–315.
Article CAS PubMed Google Scholar
Garrido-Mesa N, Zarzuelo A, Gálvez J . Minocycline: far beyond an antibiotic. Br J Pharmacol 2013; 169: 337–352.
Article CAS PubMed PubMed Central Google Scholar
Oya K, Kishi T, Iwata N . Efficacy and tolerability of minocycline augmentation therapy in schizophrenia: a systematic review and meta-analysis of randomized controlled trials. Hum Psychopharmacol 2014; 29: 483–491.
Article CAS PubMed Google Scholar
Bonjoch L, Gea-Sorli S, Jordan J, Closa D . Minocycline inhibits peritoneal macrophages but activates alveolar macrophages in acute pancreatitis. J Physiol Biochem 2015; 71: 839–846.
Article CAS PubMed Google Scholar
Kobayashi K, Imagama S, Ohgomori T, Hirano K, Uchimura K, Sakamoto K et al. Minocycline selectively inhibits M1 polarization of microglia. Cell Death Dis 2013; 4: e525.
Article CAS PubMed PubMed Central Google Scholar
Yrjänheikki J, Keinänen R, Pellikka M, Hökfelt T, Koistinaho J . Tetracyclines inhibit microglial activation and are neuroprotective in global brain ischemia. Proc Natl Acad Sci USA 1998; 95: 15769–15774.
Article PubMed PubMed Central Google Scholar
Moller T, Bard F, Bhattacharya A, Biber K, Campbell B, Dale E et al. Critical data-based re-evaluation of minocycline as a putative specific microglia inhibitor. Glia 2016; 64: 1788–1794.
Article PubMed Google Scholar
Erny D, Hrabe de Angelis AL, Jaitin D, Wieghofer P, Staszewski O, David E et al. Host microbiota constantly control maturation and function of microglia in the CNS. Nat Neurosci 2015; 18: 965–977.
Article CAS PubMed PubMed Central Google Scholar
Brown GC, Neher JJ . Microglial phagocytosis of live neurons. Nat Rev Neurosci 2014; 15: 209–216.
Article CAS PubMed Google Scholar
Sierra A, Abiega O, Shahraz A, Neumann H . Janus-faced microglia: beneficial and detrimental consequences of microglial phagocytosis. Front Cell Neurosci 2013; 7: 6.
Article CAS PubMed PubMed Central Google Scholar
Minten C, Terry R, Deffrasnes C, King NJC, Campbell IL . IFN regulatory factor 8 is a key constitutive determinant of the morphological and molecular properties of microglia in the CNS. PLoS ONE 2012; 7: e49851.
Article CAS PubMed PubMed Central Google Scholar
Cosenza-Nashat M, Zhao ML, Suh HS, Morgan J, Natividad R, Morgello S et al. Expression of the translocator protein of 18 kDa by microglia, macrophages and astrocytes based on immunohistochemical localization in abnormal human brain. Neuropathol Appl Neurobiol 2009; 35: 306–328.
Article CAS PubMed Google Scholar
Schwanhausser B, Busse D, Li N, Dittmar G, Schuchhardt J, Wolf J et al. Global quantification of mammalian gene expression control. Nature 2011; 473: 337–342.
Article PubMed CAS Google Scholar
Giovanoli S, Notter T, Richetto J, Labouesse MA, Vuillermot S, Riva MA et al. Late prenatal immune activation causes hippocampal deficits in the absence of persistent inflammation across aging. J Neuroinflammation 2015; 12: 221.
Article PubMed PubMed Central CAS Google Scholar
Giovanoli S, Weber-Stadlbauer U, Schedlowski M, Meyer U, Engler H . Prenatal immune activation causes hippocampal synaptic deficits in the absence of overt microglia anomalies. Brain Behav Immun 2016; 55: 25–38.
Article CAS PubMed Google Scholar
Monji A, Kato TA, Mizoguchi Y, Horikawa H, Seki Y, Kasai M et al. Neuroinflammation in schizophrenia especially focused on the role of microglia. Prog Neuropsychopharmacol Biol Psychiatry 2013; 42: 115–121.
Article CAS PubMed Google Scholar
Juckel G, Manitz MP, Brune M, Friebe A, Heneka MT, Wolf RJ . Microglial activation in a neuroinflammational animal model of schizophrenia—a pilot study. Schizophr Res 2011; 131: 96–100.
Article PubMed Google Scholar
Matcovitch-Natan O, Winter DR, Giladi A, Vargas Aguilar S, Spinrad A, Sarrazin S et al. Microglia development follows a stepwise program to regulate brain homeostasis. Science 2016; 353: aad8670.
Article CAS PubMed Google Scholar
Doorn KJ, Breve JJ, Drukarch B, Boddeke HW, Huitinga I, Lucassen PJ et al. Brain region-specific gene expression profiles in freshly isolated rat microglia. Front Cell Neurosci 2015; 9: 84.
Article PubMed PubMed Central CAS Google Scholar
Grabert K, Michoel T . Microglial brain region-dependent diversity and selective regional sensitivities to aging. Nat Neurosci 2016; 19: 504–516.
Article CAS PubMed PubMed Central Google Scholar
Olson JK . Immune response by microglia in the spinal cord. Ann N Y Acad Sci 2010; 1198: 271–278.
Article CAS PubMed Google Scholar
Hanamsagar R, Bilbo SD . Sex differences in neurodevelopmental and neurodegenerative disorders: focus on microglial function and neuroinflammation during development. J Steroid Biochem Mol Biol 2016; 160: 127–133.
Article CAS PubMed Google Scholar
Walker DG, Lue LF . Immune phenotypes of microglia in human neurodegenerative disease: challenges to detecting microglial polarization in human brains. Alzheimers Res Ther 2015; 7: 56.
Article PubMed PubMed Central CAS Google Scholar
Ulrich JD, Finn MB, Wang Y, Shen A, Mahan TE, Jiang H et al. Altered microglial response to Abeta plaques in APPPS1-21 mice heterozygous for TREM2. Mol Neurodegener 2014; 9: 20.
Article PubMed PubMed Central CAS Google Scholar
Xiang X, Werner G, Bohrmann B, Liesz A, Mazaheri F, Capell A et al. TREM2 deficiency reduces the efficacy of immunotherapeutic amyloid clearance. EMBO Mol Med 2016; 8: 992–1004.
Article CAS PubMed PubMed Central Google Scholar
Wilkinson B, Koenigsknecht-Talboo J, Grommes C, Lee CY, Landreth G . Fibrillar beta-amyloid-stimulated intracellular signaling cascades require Vav for induction of respiratory burst and phagocytosis in monocytes and microglia. J Biol Chem 2006; 281: 20842–20850.
Article CAS PubMed Google Scholar
Douaud G, Groves AR, Tamnes CK, Westlye LT, Duff EP, Engvig A et al. A common brain network links development, aging, and vulnerability to disease. Proc Natl Acad Sci USA 2014; 111: 17648–17653.
Article CAS PubMed PubMed Central Google Scholar
Hong S, Beja-Glasser VF, Nfonoyim BM, Frouin A, Li S, Ramakrishnan S et al. Complement and microglia mediate early synapse loss in Alzheimer mouse models. Science 2016; 352: 712–716.
Article CAS PubMed PubMed Central Google Scholar
Kempermann G . The neurogenic reserve hypothesis: what is adult hippocampal neurogenesis good for? Trends Neurosci 2008; 31: 163–169.
Article CAS PubMed Google Scholar

Download references

Acknowledgements

We thank Dr Larissa Bulavina and Philipp Jordan (Philipp.Jordan@mdc-berlin.de) for graphic preparations, and Dr Frederick Schwaller for proofreading the manuscript. We also thank Dr Kajetan Bentele for the discussion on computational analysis. The study was supported by the DFG grants SFB-TR43 (granted to HK and SAW) and WO 1418/3-1 (granted to SAW).

Author contributions

DM performed the experiments, performed data analysis and interpretation and prepared the manuscript. AI performed bioinformatics analysis contributed to data interpretation and manuscript preparation. CF performed RNA extraction and library preparation and contributed to data interpretation. PJ performed and analyzed the phagocytosis experiments. DG performed microglia isolation and sample preparation. AB performed the experiments (ELISA). WS optimized microglia isolation protocol. PP contributed to data interpretation. WD-C performed autoradiography experiments, contributed to data interpretation. PB consulted on autoradiography experiments. SH consulted on study design, radioligand preparation and autoradiography experiments. MP consulted on study design, radioligand preparation and autoradiography experiments. OS consulted on study design, radioligand preparation and autoradiography experiments. TLR prepared the radioligand. BJLE contributed to data interpretation. EWGMB contributed to data interpretation. BK contributed to data interpretation. DB contributed to data interpretation. AP consulted on RNA-sequencing setup. HK provided financial support and contributed to data interpretation and manuscript preparation. SAW initiated the study; designed the experiments; and contributed to data analysis, interpretation and manuscript preparation.

Author information

D Mattei and A Ivanov: These two authors contributed equally to this work.

Authors and Affiliations

Cellular Neurocience, Max-Delbrueck-Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany
D Mattei, P Jordan, D Guneykaya, A Buonfiglioli, H Kettenmann & S A Wolf
Core Unit Bioinformatics, Berlin Institute of Health, Berlin, Germany
A Ivanov & D Beule
Charite Medical University, Berlin, Germany
A Ivanov
Epigenetic Regulation and Chromatin Architecture Group, Berlin Institute for Medical Systems Biology, Max-Delbrück Centre for Molecular Medicine in the Helmholtz Association, Berlin, Germany
C Ferrai & A Pombo
Institute of Cell Biology and Neurobiology, Charité-Universitaetsmedizin, Berlin, Germany
A Buonfiglioli
Department of Neuroscience, Section Medical Physiology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands
W Schaafsma, B J L Eggen & E W G M Boddeke
Laboratory of Molecular Neurobiology, Nencki Institute of Experimental Biology, Warsaw, Poland
P Przanowski & B Kaminska
Department of Neuroradiopharmaceuticals, Institute of Radiopharmaceutical Cancer Research, Research Site Leipzig, Helmholtz-Zentrum Dresden-Rossendorf, Leipzig, Germany
W Deuther-Conrad & P Brust
Department of Nuclear Medicine, University of Leipzig, Leipzig, Germany
S Hesse, M Patt & O Sabri
Integrated Treatment and Research Centre (IFB) Adiposity Diseases, University of Leipzig, Leipzig, Germany
S Hesse
Department of Nuclear Medicine, Hannover Medical School, Hannover, Germany
T L Ross
Max-Delbrueck-Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany
D Beule

Authors

D Mattei
View author publications
You can also search for this author in PubMed Google Scholar
A Ivanov
View author publications
You can also search for this author in PubMed Google Scholar
C Ferrai
View author publications
You can also search for this author in PubMed Google Scholar
P Jordan
View author publications
You can also search for this author in PubMed Google Scholar
D Guneykaya
View author publications
You can also search for this author in PubMed Google Scholar
A Buonfiglioli
View author publications
You can also search for this author in PubMed Google Scholar
W Schaafsma
View author publications
You can also search for this author in PubMed Google Scholar
P Przanowski
View author publications
You can also search for this author in PubMed Google Scholar
W Deuther-Conrad
View author publications
You can also search for this author in PubMed Google Scholar
P Brust
View author publications
You can also search for this author in PubMed Google Scholar
S Hesse
View author publications
You can also search for this author in PubMed Google Scholar
M Patt
View author publications
You can also search for this author in PubMed Google Scholar
O Sabri
View author publications
You can also search for this author in PubMed Google Scholar
T L Ross
View author publications
You can also search for this author in PubMed Google Scholar
B J L Eggen
View author publications
You can also search for this author in PubMed Google Scholar
E W G M Boddeke
View author publications
You can also search for this author in PubMed Google Scholar
B Kaminska
View author publications
You can also search for this author in PubMed Google Scholar
D Beule
View author publications
You can also search for this author in PubMed Google Scholar
A Pombo
View author publications
You can also search for this author in PubMed Google Scholar
H Kettenmann
View author publications
You can also search for this author in PubMed Google Scholar
S A Wolf
View author publications
You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to S A Wolf.

Ethics declarations

Competing interests

The authors declare no conflict of interest.

Additional information

Supplementary Information accompanies the paper on the Translational Psychiatry website

Supplementary information

Supplementary Table 1 (XLSX 3556 kb)

Supplementary Table 2 (XLSX 19 kb)

Supplementary Material 1 (PDF 44 kb)

Supplementary Figure 1 (JPG 197 kb)

Supplementary Figure 2 (JPG 214 kb)

Supplementary Figure 3 (JPG 200 kb)

Supplementary Figure 4 (JPG 260 kb)

Supplementary Figure 5 (JPG 225 kb)

Rights and permissions

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

Reprints and permissions

About this article

Cite this article

Mattei, D., Ivanov, A., Ferrai, C. et al. Maternal immune activation results in complex microglial transcriptome signature in the adult offspring that is reversed by minocycline treatment. Transl Psychiatry 7, e1120 (2017). https://doi.org/10.1038/tp.2017.80

Download citation

Received: 07 December 2016
Revised: 04 February 2017
Accepted: 23 February 2017
Published: 09 May 2017
Issue Date: May 2017
DOI: https://doi.org/10.1038/tp.2017.80

This article is cited by

Noteworthy perspectives on microglia in neuropsychiatric disorders
- Hongrui Zhu
- Ao Guan
- Sheng Wang
Journal of Neuroinflammation (2023)
Neurofibromatosis type 1-dependent alterations in mouse microglia function are not cell-intrinsic
- Francesca Logiacco
- Laura Cathleen Grzegorzek
- Marcus Semtner
Acta Neuropathologica Communications (2023)
Behavioral and cellular responses to circadian disruption and prenatal immune activation in mice
- Tara C. Delorme
- William Ozell-Landry
- Lalit K. Srivastava
Scientific Reports (2023)
Microglia and microbiome in schizophrenia: can immunomodulation improve symptoms?
- Georg Juckel
- Nadja Freund
Journal of Neural Transmission (2023)
Microglial contribution to the pathology of neurodevelopmental disorders in humans
- Rugile Matuleviciute
- Elizabeth T. Akinluyi
- David A. Menassa
Acta Neuropathologica (2023)