Figure 1 : Unpredictable chronic mild stress (UCMS) induces despair behavior and microbiota dysregulation.

From: Microbiota alteration is associated with the development of stress-induced despair behavior

Figure 1

(a) Experimental design. (b) Quantification of escape behavior in the forced swim test (n = 11 naïve and 12 stressed; representative of 3 independent experiments; two-tailed t-test with Welch’s correction, **p < 0.01; mean ± s.e.m.). (c) Total bacterial load quantification by qRT-PCR of 16S rRNA (n = 17 samples per group; two-tailed t-test; mean ± s.e.m.). (d) Principal coordinate analysis of microbiome communities in naïve and stressed mice. Analysis based on 2 UCMS experiments (n = 12 naïve and 16 stressed; representative of 2 sequencing experiments) (e) Representative graphs of bacterial class distribution in individual subjects show a decrease in bacilli (yellow) (n = 5 naïve and 6 stressed; representative of 2 sequencing experiments).