Figure 7: Activation of Y406A on the surface of Caco-2 cells.

From: Engineering a pH responsive pore forming protein

Figure 7: Activation of Y406A on the surface of Caco-2 cells.
Figure 7

(a) Cells exposed to Y406A and washed with the medium at two different pH values. Left column, differential interference contrast image of Caco-2 monolayer superimposed with fluorescent image; Right column, fluorescence microscopy of the same monolayer. (b) Quantification of fluorescence. Lines 1 and 2, positive and negative control, respectively, at pH 7.4; Lines 3 and 4, positive and negative control, respectively, at pH 6; Line 5, Caco-2 cells washed with medium at pH 7.4; Line 6, Caco-2 cells washed with medium at pH 6.