Figure 1 : Computational pipeline of circRNA profiling.

From: Acfs: accurate circRNA identification and quantification from RNA-Seq data

Figure 1

(a) An example of a circRNA (colored in orange) originated from a multi-exonic gene locus (black squares). The red vertical bar on the circRNA marks the BSJ. (b) Schematic workflow of acfs, ovals denote processes for fusion circRNA identification, dashed boxes denote optional input. (c) Determination of the BSJ using circ_NEIL3 as an example. The BSJ supporting read can be partitioned in three manners (colored in orange) due to the sequence similarity at the splice sites. Exon sequences are shown in upper case, and intron sequences are shown in lower case. Splice strength is estimated for each of the potential splice sites using the maxEnt model. One partition pattern (red dash-line) is predicted to be generated by the splicing machinery and is reported as the BSJ.