Figure 1 : Spatial heterogeneity of lymphocytic infiltrate captured by biopsies varies among patients.

From: Biopsy variability of lymphocytic infiltration in breast cancer subtypes and the ImmunoSkew score

Figure 1

(A) Illustration of the virtual biopsy pipeline. Three whole-tumour H&E stained sections to represent a primary breast tumour in the METABRIC study. Virtual, non-overlapping TMA cores were taken from this tumour. (B) An example of a virtual TMA core image, and identified cells after automated image analysis (green dots: cancer cells; red dots: stromal cells; blue dots: lymphocytes). (C,D) Illustration of tumour samples with low and high spatial correlation in lymphocytic infiltrate. For both samples, the left panel demonstrates the TMA cores sampled from a tissue section. Black coloured circle represents the reference core to compare against, the variation of colours between red and green shows the increase in distance from reference core (red means TMAs are closer to reference core while green means the core is relatively far away from reference core). Size of each circle scales with the number of cells in each TMA core. The middle panel plots the distance between each core from reference core against the difference in lymphocyte ratio between each core and the reference core. The right panel shows the marginal distribution of lymphocyte ratio in the cores.