Figure 2 : High glucose upregulates ADAM9 expression while MiR-126 mimic inhibits its expression in RAW 264.7 cells.

From: MicroRNA-126 overexpression rescues diabetes-induced impairment in efferocytosis of apoptotic cardiomyocytes

Figure 2

(A) Quantitative real time PCR data showing increase in ADAM9 mRNA expression in RAW 264.7 cells treated with high glucose compared to normoglycemia (normal glucose) treated cells. n = 3. *P < 0.01. (B) Representative western blot and densitometry (C) showing a corresponding increase in ADAM9 protein expression in high glucose-treated cells. (D) Reduced ADAM9 mRNA levels in RAW 264.7 cells transfected with miR-126 mimic compared to control mimic treated cells; n = 3, **P < 0.05. (E) Representative western blot and densitometry (F) showing ADAM9 protein expression decreases in miR-126 mimic treated RAW 264.7 cells compared to controls. (G) Quantitative dual-luciferase reporter assay showing decrease in ADAM9 3′UTR-mediated luciferase activity in miR126 mimic treated cells when compared to control mimic treated cells; n = 3. ***P < 0.01. Data normalized to GAPDH.