Table 6: Primers used in the study.

From: Development and characterisation of highly antibiotic resistant Bartonella bacilliformis mutants

Table 6: Primers used in the study.
PrimerTargetSequence (5′-3′)Ann (°C)Size (bp)Ref.
Resistance to quinolones
gyrA-FgyrACAT GCG ATG AAT GAA ATG GGA CTT TTG5523319
gyrA-RAAA CGA CAT TCC GTG TAA CGC ATC GC
gyrB-FgyrBCTG AAG TCC GTC CAA TTG TT*48634TS
gyrB-RTCT TCA AAT GCT GCT TCA TT*
parE-FparECAA TAC GTG ATC CTT TCG AT*47564TS
parE-RTTC CTC CTT GTG ATA TTC TG*
parC-FparCTCT TAT GCT AAG TGT GCA CGG A5534914
parC-RTAC CAA CAG CAA TCC CTG AAG AA
Resistance to macrolides
rplD_FrplDAGA AGT CTC TGT AGC TGA GGG49688TS
rplD_RACT GGA CTG ACA ATT ACA TCA
rplV_FrplVCTG GAC TGA CAA TTA CAT CAT49702TS
rplV_RGGC GAC TCC AAT AGC AGA AG
Resistance to macrolides and amphenicols
23S_rRNA_F23S rRNAAGT GAA ATT GAA TTC CCC46780TS
23S_rRNA_RGGA ATA CTC GTT TTC AGG T
Resistance to rifamycins
rpoB-FrpoBGAT GAT ATC GAC AAT CTT GGT A**49818TS
rpoB-RGCA GCA CCT GAA TCA CGA GCC
Bacterial identification
16SBartonella-F16S rRNACCT TCA GTT MGG CTG GAT C5543859
16SBartonella-RGCC YCC TTG CGG TTA GCA CA
  1. TS. This Study.
  2. *These primers are modifications of those described by Angelakis et al.43.
  3. **This primer is a modification of the BarpoBF primer designed by Biswas et al.18.