After the discovery of small interference RNA (siRNA), nanostructured siRNA delivery systems have been introduced to achieve an efficient regulation of the target gene expression. Here we report a new siRNA-generating two dimensional nanostructure in a formation of nanosized sheet. Inspired by tunable mechanical and functional properties of the previously reported RNA membrane, siRNA nanosized sheets (siRNA-NS) with multiple Dicer cleavage sites were prepared. The siRNA-NS has two dimensional structure, providing a large surface area for Dicer to cleave the siRNA-NS for the generation of functional siRNAs. Furthermore, downregulation of the cellular target gene expression was achieved by delivery of siRNA-NS without chemical modification of RNA strands or conjugation to other substances.
Attention towards ribonucleic acid (RNA) has naturally grown due to its myriad of biological potentials. In addition to messenger RNA (mRNA) carrying genetic information within organisms, non-coding RNAs such as transfer RNA (tRNA), ribosomal RNA (rRNA), short-interfering RNA (siRNA) and small nuclear RNA (snRNA) can also serve particular roles in biological system1. In addition, RNA has been recently used as a generic material for nano- and micro-architectures in the scope of materials engineering to utilize a wide range of its functions2,3,4. In particular, after the discovery of RNA interference (RNAi) in 19985, RNA architectures carrying Dicer substrates have been widely investigated. For Dicer enzyme to generate small interference RNA (siRNA) or micro RNA (miRNA) to induce gene silencing effect, RNA nanostructures have been designed to bear double-stranded RNA (dsRNA). With this consideration, various RNA structures were developed with the shapes of dumbbell6,7,8, branched multimer9 and dendrimer10. By rational design, siRNAs generated from the artificial RNA structures can inhibit expression of virtually any gene in target-specific manner11.
In particular, after the development of our self-assembled RNAi-inducing microsponges bearing multiple hairpin structures as siRNA precursors12, our group has intensively explored for controllable synthesis of RNAi-inducing microscopic constructs to achieve high therapeutic efficacy. In the synthesis of RNAi microsponge, enzymatic approach was adapted to generate multiple copies of long RNA strands as well as to induce self-assembly of the strands. Moreover, size-controlling of RNA nanoparticles was successfully demonstrated recently via adjusting the concentrations of RNA polymerase in enzymatic replication reaction13. Building on previous achievements, a novel approach to prepare RNA membrane without polymer supports or complexation was also recently introduced14. This was the first example of a macroscopic RNA membrane, which is composed of RNA strands and has tunable mechanical properties and biological functions. In this study, we also demonstrated successful release of chemical drugs from our RNA membranes. In addition, we found that two-dimensional structure of the membrane was advantageous for effective siRNA generation by Dicer.
Inspired by membrane’s structural property, here we report the preparation of siRNA nanosized sheet (siRNA-NS) with lateral dimensions of <500 nm. To boost therapeutic potential of two-dimensional RNA structure with fully exploiting its material properties, such as large surface area and tunable biological functions, our RNA membrane was embedded with siRNA strands and fractured into nanosheets via ultrasonication to induce RNAi in cytoplasm (Fig. 1). By controlling degree of base-pairing, only functional parts, potential siRNA, are designed to form duplexes via hybridization. In this design, we can eliminate undesired formation of short duplexes that are not functional (Fig. 1a). From this partially complementary RNA membrane with bubbles between potential siRNAs that is relatively thinner and flexible, we could easily generate siRNA-NS via ultrasonication.
Results and Discussion
To synthesize siRNA-NS for mediating RNAi, two types of circular DNAs with rationally designed sequences were first generated using the method reported elsewhere14,15. Circular DNA1 and circular DNA2 depicted in Fig. 1a are rationally designed to encode sense strand and antisense strand for anti-GFP siRNA, respectively (Table 1). Similar to the method reported previously14, both circular DNAs bear promoter region for T7 RNA polymerase, thus two kinds of RNA strands can be generated repeatedly via complementary rolling circle transcription. By rational design, two partially complementary circular DNAs were used to generate repeating siRNA duplexes as fabrics for siRNA-NS. To be specific, several transcripts by RCT are hybridized and entangled with other neighbouring transcripts, then finally condensed into the membrane formulation through evaporation-induced self-assembly. By extensive ultrasonication, RNA membrane was easily torn up into the nanosized sheets (Fig. 1b). The digital camera image in Fig. 1b clearly shows the tearing process of RNA membrane to a small piece of membranes within several minutes of sonication.
To closely investigate the tearing process, each step of the process was observed by scanning electron microscope (SEM) including the image of intact RNA membrane (Fig. 2a). When introduced with ultrasonic pulses, RNA membrane starts to be torn in a similar way of a sheet of paper being torn. After a brief ultrasonication for 20 s, RNA membrane become fractured into large pieces still visible by naked eyes when stained with GelRed. After further sonication, dominating products were microsized (Fig. 2b). The high magnification SEM image of the split site of the RNA membrane in the process of production of siRNA-NS reveals that the RNA membrane is composed of multiple thinner layers. By ultrasonic impulses, the layers fall off from the membrane, and are simultaneously torn into small pieces. With further ultrasonication, sediments and floating pieces coexist in the reaction tube. After 20 min of vigorous sonication, the sheets finally become under 500 nm-sized, which is confirmed by SEM (Fig. 2c,d) and dynamic light scattering (DLS, Fig. 2e). As a result of sonication, morphology of siRNA-NS was varied. Further analysis with energy-dispersive X-ray spectroscopy (EDX) revealed siRNA-NS is composed of carbon (C), nitrogen (N), oxygen (O) and phosphorous (P), indicating siRNA-NS is made of RNA strands (Fig. 2f).
As illustrated in Fig. 3a, each siRNA-NS has numerous cleavage sites, and the cleaved product can later serve as functional siRNA. To examine the potential of siRNA-NS to generate embedded siRNAs, Dicer enzyme-mediated in vitro generation of siRNA duplexes was analysed by polyacrylamide gel electrophoresis (Fig. 3b,c). 42.7% of total RNA was cleaved to the length of ~23 bp by 24 h (Fig. 3f), and 85.5% of the total RNA was cleaved to the length of ~23 bp by 48 h (Fig. 3g). Compared to the micro-sized particle formulated short hairpin RNAs (shRNA) which was previously reported to generate 21% of cleavable siRNA strands12, siRNA-NS has shown capability of high-yield generation of siRNAs. This is because thin sheet-like 2-D structure of the siRNA-NS provides a large surface area for enzyme-substrate contact which enables the cleaving enzyme to work efficiently. However, biological stability of siRNA-NS was comparably higher than naked siRNA mainly due to densely packed internal structure of the nanosheets (see Supplementary Fig. S1).
To examine therapeutic efficacy of siRNA-NS, HeLa cells expressing enhanced green fluorescence protein (HeLa-GFP) were treated with anti-GFP siRNA-NS. Decrease of green fluorescence intensities was confirmed at 24 h after the treatment of anti-GFP siRNA-NS, showing maximum silencing efficiency with 500 pM (Fig. 4a). The cellular GFP level of the anti-GFP siRNA-NS treated cells was reduced to 50% in 24 h, while scrambled siRNA-NS had negligible effect on the cellular GFP level (Fig. 4b). Cytometry analysis also presents green fluorescence intensities of the HeLa-GFP cells treated with anti-GFP siRNA-NS were reduced (Fig. 4c). Moreover, the target gene silencing effect of siRNA-NS persisted until 6 days post-transfection (see Supplementary Fig. S2), indicating a capability of long-term gene repression by delivering siRNA-NS.
In conclusion, nanosized RNA sheets were synthesized successfully with potential siRNA strands. For producing siRNA-NS, partially complementary RNA membrane which has 46 base-pairings out of 92 bases was chosen, so that one turn of T7 RNA polymerase around the circular DNA can result in two potential siRNA regions. Through repetitive polymerization on the template DNAs, long RNA strands can be generated, which results in multiple Dicer cleavage sites. In addition, this RNA membrane could be easily torn into nanosized sheets by ultrasonication because the membrane has flexible mechanical property. We have also demonstrated efficient generation of siRNAs from siRNA-NS in presence of Dicer enzyme and fast gene silencing effect in cells, which suggests siRNA-NS can be utilized as a new platform for efficient siRNA delivery. Therefore, over the advantage of using spherical structures, two dimensional nanosized RNA sheet could bolster therapeutic efficacy of RNA-based materials.
Materials and reagents
DNA oligonucleotides were purchased from Integrated DNA Technologies (IDT). T4 DNA ligase (Cat. No. M1804) was purchased from Promega. T7 RNA polymerase (Cat. No. M0251L), 10X RNA polymerase reaction buffer (Cat. No. B9012S) and ribonucleotide solution mix (Cat. No. N0466S) were purchased from New England BioLabs. GelRed (Cat. No. 41003) was purchased from Biotium. Dulbecco’s Modified Eagle’s Medium (DMEM, Cat. No. LM 001-10) and fetal bovine serum (FBS, Cat. No. S 101-01) were purchased from Welgene. 100 X Antibiotic-Antimycotic (Cat. No. 15240-062), trypsin-EDTA (Cat. No. R-001-100) and Dulbecco’s phosphate-buffered saline (DPBS, Cat. No. 14190-144) were purchased from Gibco. Transfection reagent (TransIT-X2 Dynamic Delivery System, Cat. No. MIR 6000) was purchased from Mirus.
Circularization of linear DNA
Phosphorylated 92 base-long linear DNA and 22 base-long primer DNA at the final concentration of 10 μM were mixed in nuclease-free water. For denaturation and annealing, the mixture was heated at 95 °C for 2 min and slowly cooled to 25 °C for 1 h using thermal cycler (Bio-Rad). To ligate the nick in the circularized DNA, the solution was incubated overnight with 0.03 U μl−1 of T4 DNA ligase and ligase buffer (30 mM Tris-HCl (pH 7.8), 10 mM MgCl2, 10 mM DTT and 1 mM ATP).
Generation of siRNA-NS
For complementary rolling circle transcription process, circularized DNA (final concentration of 3 μM) and complementary circular DNA (final concentration of 3 μM) were mixed with 15 mM of ribonucleotide solution mix, reaction buffer (60 mM Tris-HCl, 9 mM MgCl2, 3 mM spermidine and 1.5 mM dithiothreitol) and 5 U μl−1 of T7 RNA polymerase. The reaction solution was incubated for 20 h at 37 °C, then evaporation-induced self-assembly was carried out overnight14. After removal of the remaining solution, RNA membrane was washed three times with nuclease-free water. Then, RNA membrane was resuspended in 100 μl of nuclease-free water. The samples were placed on a water bath-type ultrasonicator, and ultrasonication pulses were applied to the samples for 20 min. The frequency and output of the sonication were 28 kHz and 50 watts, respectively, and the temperature was maintained at 4 °C. A small portion of micro-sized sheets were removed after centrifugation at 8000 rpm for 3 min.
Characterization of siRNA-NS
S-5000 H (Hitachi) field emission scanning electron microscope (FE-SEM) was used to obtain high resolution images of siRNA-NS, and FE-SEM based energy dispersion X-ray (EDX) was used to analyse chemical compositions of siRNA-NS. The size and surface charge of siRNA-NS were measured using Malvern Zetasizer Nano-ZS90 and analysed with Zetasizer software (Malvern Instruments). Each sample was prepared immediately before use and diluted with nuclease-free water. All measurements were carried out at 25 °C, and three measurements with at least 10 sub-runs were performed for each sample.
siRNA generation from siRNA-NS
For siRNA generation from siRNA-NS, siRNA-NSs were incubated with 0.1 U μl−1 of recombinant human Dicer enzyme and reaction buffer (1 mM ATP, 2.5 mM MgCl2, 40% Dicer reaction buffer) at 37 °C for 24 h or 48 h. The cleaved products were examined by 20% non-denaturing PAGE in 0.5X Tris-borate-EDTA (TBE) buffer carried out at 120 V for 60 min. Then, the gels were stained with 1X GelRed in 0.5X TBE and analysed under UV with Image Lab software (Bio-Rad).
In vitro gene silencing assay
HeLa-GFP cells were grown in DMEM supplemented with 10% FBS, 100 U ml−1 of penicillin, 100 μg ml−1 of streptomycin and 1% Antibiotic-Antimycotic at 37 °C in a humidified atmosphere supplemented with 5% CO2. The cells were passaged routinely to maintain exponential growth. One day prior to transfection (~90% confluence), the cells were trypsinized, diluted with fresh medium and transferred to 96-well plates (7000 cells per well). The siRNA-NSs were covered with the transfection reagent prior to transfection according to the manufacturer’s instruction, then the cells were treated with the covered siRNA-NS for 24 or 48 h. For cytometry analysis, HeLa-GFP cells were seeded at 24-well plates at the density of 50000 cells per well. The cells were treated with 500 pM of the covered siRNA-NS for 12 h or 24 h. The cells were first stained with Hoechst33342 and propidium iodide, then analysed with NucleoCounter NC-3000 (ChemoMetec).
How to cite this article: Kim, H. et al. Generation of siRNA Nanosheets for Efficient RNA Interference. Sci. Rep. 6, 25146; doi: 10.1038/srep25146 (2016).
This research was supported by the Global Innovative Research Center (GiRC) Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (NRF-2012K1A1A2A01056093) and also supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (NRF-2015R1A1A1A05001174).
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