RCF1-dependent respiratory supercomplexes are integral for lifespan-maintenance in a fungal ageing model

Fischer, Fabian; Filippis, Christodoulos; Osiewacz, Heinz D.

doi:10.1038/srep12697

Download PDF

Article
Open access
Published: 29 July 2015

RCF1-dependent respiratory supercomplexes are integral for lifespan-maintenance in a fungal ageing model

Fabian Fischer¹,
Christodoulos Filippis¹ &
Heinz D. Osiewacz¹

Scientific Reports volume 5, Article number: 12697 (2015) Cite this article

1691 Accesses
21 Citations
1 Altmetric
Metrics details

Subjects

Abstract

Mitochondrial respiratory supercomplexes (mtRSCs) are stoichiometric assemblies of electron transport chain (ETC) complexes in the inner mitochondrial membrane. They are hypothesized to regulate electron flow, the generation of reactive oxygen species (ROS) and to stabilize ETC complexes. Using the fungal ageing model Podospora anserina, we investigated the impact of homologues of the Saccharomyces cerevisiae respiratory supercomplex factors 1 and 2 (termed PaRCF1 and PaRCF2) on mtRSC formation, fitness and lifespan. Whereas PaRCF2’s role seems negligible, ablation of PaRCF1 alters size of monomeric complex IV, reduces the abundance of complex IV-containing supercomplexes, negatively affects vital functions and shortens lifespan. PaRcf1 overexpression slightly prolongs lifespan, though without appreciably influencing ETC organization. Overall, our results identify PaRCF1 as necessary yet not sufficient for mtRSC formation and demonstrate that PaRCF1-dependent stability of complex IV and associated supercomplexes is highly relevant for maintenance of the healthy lifespan in a eukaryotic model organism.

Messenger RNA transport on lysosomal vesicles maintains axonal mitochondrial homeostasis and prevents axonal degeneration

Article Open access 10 April 2024

Small extracellular vesicles from young plasma reverse age-related functional declines by improving mitochondrial energy metabolism

Article Open access 16 April 2024

A guide to cell death pathways

Article 18 December 2023

Introduction

Convincing evidence exists that mitochondrial dysfunction plays a key role in biological ageing and various age-related human pathologies^1,2,3,4,5. It has long been suggested that the root cause for the progressive decline of mitochondrial function is the age-dependent accumulation of reactive oxygen species, which inevitably arise during oxidative phosphorylation⁶. The primary ROS superoxide anion (O₂^.−) is produced at complexes I and III of the ETC and can give rise to the secondary ROS hydrogen peroxide which in turn can lead to the formation of the highly reactive hydroxyl radical through the Fenton reaction. Both O₂^.− and the hydroxyl radical are able to damage proteins, lipids and DNA. Consequently, all ROS are harmful to mitochondria when present in excess⁷. Recently, this narrow view of ROS solely as damaging agents has been challenged by counter-intuitive and contradictory experimental observations^8,9. These may, at least in part, result from the fact that low levels of ROS are essential for cellular signalling and to control developmental processes^10,11. A balanced generation and degradation of ROS is therefore highly important to keep biological systems functional over time. In this context, maintaining integrity of the ETC is crucial⁵.

In their seminal paper published in 2000, Schägger and Pfeiffer convincingly demonstrated the existence of stoichiometric assemblies of individual ETC complexes, so called mitochondrial respiratory supercomplexes or respirasomes, in yeast and mammalian mitochondria¹². The ‘plasticity model’ of ETC organization is based on additional observations of considerable variations in mtRSC species and hypothesizes, that individual ETC complexes and supercomplexes can exist side by side in the inner mitochondrial membrane^13,14. This view is supported by a growing number of studies demonstrating mitochondrial supercomplexes to be functional units of respiration assumed to be important for facilitating and directing electron flow by substrate channeling and consequently, to regulate the production of ETC-derived ROS. Additionally, there is evidence for a reciprocal dependence of supercomplex formation and the assembly and stabilization of individual ETC complexes, most importantly complex I^14,15,16,17.

The proteins RCF1 and RCF2, members of the ‘hypoxia-inducible gene 1’ (HIG1) protein family, were recently identified in S. cerevisiae as being important constituents of mtRSCs^18,19,20. ScRCF1 in particular was revealed as a crucial component for stabilization of the III₂IV₂ supercomplex^18,19,20,21, while ScRCF2 appears to have a less prominent function^19,20. The Rutter group also demonstrated a loss of complex IV-containing supercomplexes in mitochondria extracted from C2C12 mouse myoblast cells after knockdown of the mammalian RCF1 homologue HIG2A¹⁸.

Despite these notable insights, the in vivo relevance of mitochondrial supercomplexes remains to be clarified in detail. A destabilization of mtRSCs has already been linked to the development of at least one complex human disorder known as Barth syndrome^22,23. It seems likely, owing to the central role of mitochondria in health and disease⁵, that similar connections will be unravelled in the near future¹⁶. To this end, it is an important task to determine the relationship between altered supercomplexes, mitochondrial function and impact on the organism as a whole.

The filamentous ascomycete P. anserina is characterized by a limited lifespan, a clear mitochondrial aetiology of ageing and has been extensively analysed as a simple model for organismal ageing²⁴. Its ETC features, in contrast to that of S. cerevisiae, both complex I as well as an alternative terminal oxidase (AOX) that can be activated to bypass complexes III and IV by directly transferring electrons from ubiquinol to oxygen²⁵. In the wild type, three major supercomplexes (I₁III₂IV_0–2) can be resolved by blue native polyacrylamide gel electrophoresis (BN-PAGE)²⁶. Genetic disruptions of complexes III and/or IV in P. anserina have been shown to result in absence of the corresponding supercomplexes^26,27 and enhanced AOX-dependent respiration, with mutant strains consistently displaying increased lifespans^25,27,28,29.

Here we describe the role of RCF1 and RCF2 in P. anserina. Deletion of PaRcf2, encoding the RCF2 homologue, has no pronounced effect on the phenotype, while ablation of PaRCF1 shifts the complex IV monomer almost exclusively to a form that migrates faster during BN-PAGE than that of the wild type. In addition, the abundance of I₁III₂IV_1–2 supercomplexes is strongly reduced. Concomitant with these changes, mitochondrial integrity as well as vital characteristics of the PaRcf1 deletion strain, such as growth rate and fertility, are impaired and lifespan, despite activation of the alternative respiratory pathway, is shortened. Overall, our results identify RCF1-stabilized complex IV and complex IV-containing supercomplexes as crucial ETC components in P. anserina, establishing a novel connection between their destabilization, impaired mitochondrial function and a negative impact on health and lifespan.

Results

Deletion of PaRcf1 disturbs complex IV and supercomplexes

The genome of P. anserina is fully sequenced (http://podospora.igmors.u-psud.fr). Using BLAST searches (http://podospora.igmors.u-psud.fr/blast.php), we identified a homologue of ScRCF1 (UniProt: Q03713), murine HIG2A (UniProt: Q9CQJ1) and human HIG2A (UniProt: Q9BW72) that we termed PaRCF1 (P. anserina accession number: Pa_5_5310; UniProt: Q875C2) as well as a homologue of ScRCF2 (UniProt: P53721) termed PaRCF2 (P. anserina accession number: Pa_1_4450; UniProt: B2AAL7). PaRCF1 and PaRCF2 share an amino acid (AA) sequence identity of 26% and 27% and a similarity of 40% and 44% compared with the respective homologues of S. cerevisiae (Supplementary Fig. S1). PaRCF1 has a length of 218 AA with a predicted 18 AA N-terminal mitochondrial targeting sequence (MTS) and a calculated mass of ~22 kDa for the mature protein. Similar to ScRCF1 it contains a ‘hypoxia induced protein conserved region’ domain (HIG_1_N) and two predicted transmembrane helices (TMH). PaRCF2 has a length of 242 AA, a calculated mass of ~27 kDa and contains a HIG_1_N domain and three predicted TMHs (Fig. 1a and Supplementary Fig. S1).

PaRcf1 and PaRcf2 deletion strains (ΔPaRcf1 and ΔPaRcf2) were generated according to the method described by El-Khoury and colleagues, with the respective wild-type gene’s ORF replaced by a hygromycin B resistance gene³⁰. The deletion strains were verified by Southern blot analysis (Fig. 1b,c and Supplementary Fig. S2).

To analyse the importance of PaRCF1 and PaRCF2 for maintaining integrity of the ETC, mitochondrial protein extracts from wild type (n = 4), ΔPaRcf1 (n = 3) and ΔPaRcf2 (n = 4) were compared using BN-PAGE (Fig. 1d, Table 1 and Supplementary Fig. S3). Deletion of PaRcf2 had no discernible effect on overall ETC composition. PaRcf1 deletion, however, led to marked changes. Specifically, relative abundance of the I₁III₂IV₂ (S₂) supercomplex (0.07 ± 0.01 AU; P = 0.02 by two-tailed Student’s t-test) and of the I₁III₂IV₁ (S₁) supercomplex (0.03 ± 0.02 AU; P = 6.8E-04 by two-tailed Student’s t-test) in ΔPaRcf1 was significantly reduced, while relative abundance of the I₁III₂IV₀ (S₀) supercomplex (15.30 ± 2.58 AU; P = 0.03 by two-tailed Student’s t-test) was significantly increased. Free complex IV monomer was undetectable in mitochondrial protein extracts from ΔPaRcf1, though it should be noted that even in wild type and ΔPaRcf2 monomeric complex IV is detectable only by a diffuse and rather faint band after BN-PAGE and Coomassie staining. A complex IV ‘in-gel’ activity assay (Fig. 1e, Table 2 and Supplementary Fig. S4) was used to measure relative distribution of complex IV activity within each individual strain and confirmed the near total absence of the S₂ and S₁ supercomplexes in ΔPaRcf1. It also revealed that monomeric complex IV in ΔPaRcf1 is almost exclusively present in a faster migrating form (IV_B) than in the wild type or in ΔPaRcf2.

Table 1 Quantification of ETC complexes and supercomplexes in wild-type, ΔPaRcf1 and ΔPaRcf2 mitochondria.

Full size table

Table 2 Quantification of complex IV activity in wild-type, ΔPaRcf1 and ΔPaRcf2 mitochondria.

Full size table

Relative abundance of PaCOX2, an essential complex IV subunit encoded by the mitochondrial genome, was found to be slightly reduced in mitochondrial protein extracts from ΔPaRcf1 (0.84 ± 0.07 AU; P = 0.45 by two-tailed Student’s t-test), while in contrast being increased in those from ΔPaRcf2 (1.55 ± 0.11 AU; P = 4.7E-02 by two-tailed Student’s t-test) compared to the wild type (1.00 ± 0.18 AU; Fig. 1f,g and Supplementary Fig. S5).

ETC alterations in ΔPaRcf1 impair mitochondrial integrity

To assess the functional consequences of PaRcf1 and PaRcf2 deletion for mitochondrial respiration, we measured oxygen consumption of wild type (n = 3), ΔPaRcf1 (n = 3) and ΔPaRcf2 (n = 3) mycelium with and without the addition of the specific respiratory inhibitors KCN, inhibiting complex IV and salicylhydroxamic acid (SHAM), inhibiting AOX (Fig. 2a and Supplementary Fig. S6). Notably, total absolute oxygen consumption of ΔPaRcf1 was tendentially increased compared to the wild type (Supplementary Fig. S6). Similar effects have previously been observed in other P. anserina ETC mutants, likely reflecting inefficient oxygen utilization for ATP generation³¹. Sequential addition of both inhibitors in either order (i.e. first KCN and then SHAM or first SHAM and then KCN) almost completely inhibited oxygen consumption in all strains. Therefore, the majority of oxygen in P. anserina is consumed during mitochondrial respiration (Supplementary Fig. S6). Relative oxygen consumption after inhibition of complex IV with KCN was significantly higher in ΔPaRcf1 (0.83 ± 0.07 AU; P = 5.2E-03 by two-tailed Student’s t-test) than in wild type (0.25 ± 0.08 AU), i.e. ΔPaRcf1 exhibits, in accord with the results obtained by BN-PAGE (Fig. 1d,e and Tables 1 and 2), a decrease in complex IV-dependent respiration. In contrast, inhibition of AOX with SHAM resulted in significantly lower relative oxygen consumption in ΔPaRcf1 (0.43 ± 0.03 AU; P = 7.0E-03 by two-tailed Student’s t-test) than in wild type (0.76 ± 0.05 AU). Interestingly, the effect of complex IV inhibition by KCN in ΔPaRcf1 was more pronounced if AOX was first inhibited by SHAM and vice versa (Supplementary Fig. S6). This probably reflects an adaptive upregulation of either respiratory pathway after inhibition of the other pathway during oxygen consumption measurements.

In conclusion, these observations indicate an upregulation of the alternative respiratory pathway in ΔPaRcf1. Similar yet less pronounced changes in absolute and relative oxygen consumption after addition of KCN or SHAM were also observed in ΔPaRcf2 (Fig. 2a and Supplementary Fig. S6), despite the lack of visible alterations in ETC composition (Fig. 1d,e and Tables 1 and 2).

Comparison of PaAOX abundance in mitochondrial protein extracts from wild type (n = 4), ΔPaRcf1 (n = 4) and ΔPaRcf2 (n = 4) by western blot analysis (Fig. 2b–d and Supplementary Fig. S7) revealed significant changes only in ΔPaRcf1, where relative PaAOX abundance was considerably increased (4.89 ± 0.28 AU; P = 5.5E-03 by two-tailed Student’s t-test). As no obvious deviations in ΔPaRcf2’s phenotype from that of the wild type, apart from the changes in oxygen consumption (Fig. 2a), were identified (see also below), we subsequently focused on ΔPaRcf1.

Different P. anserina ETC mutants display decreased mitochondrial ROS and an increased lifespan concomitant with enhanced activation of the alternative respiratory pathway^25,28. Consequently, the observation that respiration in ΔPaRcf1 is primarily AOX-dependent suggested that superoxide mediated damage to proteins might be reduced in this strain. Contrary to this assumption we found that ΔPaRcf1 had a slight but nonetheless significant increase in mitochondrial protein carbonylation (1.33 ± 0.07 AU; n = 6; P = 4.6E-03 by two-tailed Student’s t-test), possibly reflecting impairments in ROS scavenging mechanisms and/or in the clearance of damaged mitochondrial proteins (Fig. 2e,f).

Western blot analyses comparing relative protein abundances in mitochondrial protein extracts from wild type (n = 4) and ΔPaRcf1 (n = 4) indeed revealed significant reductions of mitochondrial matrix proteases PaCLPP (0.20 ± 0.05 AU; P = 1.6E-05 by two-tailed Student’s t-test) and PaLON (0.31 ± 0.08 AU; P = 4.2E-03 by two-tailed Student’s t-test) as well as of mitochondrial superoxide dismutase PaSOD3 (0.24 ± 0.03 AU; P = 1.2E-04 by two-tailed Student’s t-test) and peroxiredoxin PaPRX (0.40 ± 0.08 AU; P = 2.3E-03 by two-tailed Student’s t-test) in ΔPaRcf1, while level of the chaperone PaHSP60 (1.16 ± 0.22 AU) remained unchanged (Fig. 2g,h and Supplementary Fig. S8). In addition, relative abundance of mitochondrial aconitase PaACO2 was also found to be strongly and significantly reduced (0.05 ± 0.02 AU; P = 9.0E-03 by two-tailed Student’s t-test) in the PaRcf1 deletion strain (Fig. 2g,h and Supplementary Fig. S8).

Surprisingly, despite the strong reduction of PaSOD3 protein abundance in ΔPaRcf1 by almost 80% (Fig. 2g,h), visualization of PaSOD3-activity in mitochondrial protein extracts from P. anserina wild type and ΔPaRcf1 with an SOD ‘in-gel’ activity assay revealed no observable differences between the two strains (Supplementary Fig. S9). This result suggests that the remaining PaSOD3 in ΔPaRcf1 is highly active to counteract oxidative stress.

ΔPaRcf1 is unable to maintain a healthy lifespan

To date, consequences for organismal health following a targeted genetic disruption of mitochondrial respiratory supercomplexes are scarcely studied. P. anserina RCF1, as demonstrated in this study, is critically involved in stabilizing complex IV. Concomitant with a destabilization of complex IV in ΔPaRcf1, abundance of associated supercomplexes is also strongly reduced (Fig. 1d,e and Tables 1 and 2). Presumably as a consequence thereof, mitochondrial integrity in the PaRcf1 deletion strain is negatively affected (Fig. 2a–h). To better understand the resulting impact on the organism as a whole, several vital characteristics of ΔPaRcf1 were assessed.

It is reasonable to assume that ΔPaRcf1, showing increased oxidative protein damage (Fig. 2e,f) and a reduction of key enzymes involved in ROS scavenging (Fig. 2g,h), should be more susceptible to oxidative stressors. This was indeed true for paraquat, known to generate O₂^.− at the ETC³², which almost completely inhibited growth of the PaRcf1 deletion strain already at a concentration of 80 μM. At this concentration, growth rate of wild type and ΔPaRcf2 remained nearly unaffected (Fig. 3a). Other stressors, namely H₂O₂ and CuSO₄, had no intensified effect on ΔPaRcf1 (Supplementary Fig. S10). Next, influence of 20 μM paraquat on survival of the PaRcf1 and PaRcf2 deletion strains was investigated. Previous work of our laboratory showed that, similar to what has been observed in Caenorhabditis elegans^33,34, low doses of paraquat can considerably prolong lifespan of the P. anserina wild-type strain³⁵. Interestingly, this effect was completely abrogated in ΔPaRcf1, while still being preserved in ΔPaRcf2 (Supplementary Fig. S11).

As the mammalian RCF1 homologue HIG1A is known to be transcriptionally regulated³⁶, we speculated that PaRcf1 expression might be induced by low doses of paraquat and that PaRCF1 is possibly involved in mediating paraquat-triggered lifespan extension. Relative PaRcf1 expression in the wild type was, however, not elevated after cultivation with 20 μM paraquat (Supplementary Fig. S11).

The PaRcf1 deletion strain was further characterized by female infertility (Fig. 3b) and a significant reduction of its growth rate on standard medium by 58% (0.25 ± 0.01 cm d⁻¹; n = 69; P = 2.2E-27 by two-tailed Wilcoxon rank-sum test; Fig. 3c and Table 3). Perhaps the most remarkable characteristic of ΔPaRcf1’s phenotype was a significant 40% shortening of its mean lifespan (14.5 ± 0.7 d; n = 71; P = 3.4E-19 by two-tailed Wilcoxon rank-sum test; Fig. 3d and Table 3). Deletion of PaRcf2, aside from a slightly elevated growth rate, again led to no distinct deviations from the wild type under any of the conditions tested (Fig. 3a–d and Table 3).

Table 3 Lifespan and growth rate of wild type, ΔPaRcf1, ΔPaRcf2, ΔPaRcf1/PaRcf1-6xHis and PaRcf1-6xHis_Oex.

Full size table

To determine the specificity of the observed effects, we complemented ΔPaRcf1 by introducing a C-terminally 6xHis-tagged variant of PaRcf1 under control of the native promotor and terminator. Presence of the recombinant gene in the complemented strain (ΔPaRcf1/PaRcf1-6xHis) was verified by Southern blot analysis (Supplementary Fig. S12). Relative expression of PaRcf1-6xHis was similar to that of PaRcf1 in the wild type (Supplementary Fig. S12) and the recombinant protein could be detected in mitochondrial protein extracts from ΔPaRcf1/PaRcf1-6xHis (Supplementary Fig. S12). The complemented strain was again fertile and displayed a wild-type like growth rate (Fig. 3c and Table 3) and lifespan (Fig. 3d and Table 3).

Finally, since absence of PaRCF1 had such a dramatic negative effect on health and lifespan, we investigated whether overexpression of PaRcf1 might be beneficial for P. anserina. A strain expressing PaRcf1-6xHis under control of a constitutive promotor in the wild-type background (PaRcf1-6xHis_OEx) was generated and verified by Southern and western blot analysis (Supplementary Fig. S13). Relative PaRcf1 expression (20-fold; P = 3.6E-02 by two-tailed Student’s t-test) and relative PaRCF1-6xHis protein abundance (17-fold; P = 6.5E-17 by two-tailed Student’s t-test) were significantly elevated compared with the wild type or ΔPaRcf1/PaRcf1-6xHis, confirming stable overexpression of the construct (Supplementary Fig. S13). While paraquat resistance of PaRcf1-6xHis_OEx remained essentially unchanged (Supplementary Fig. S13), both its growth rate (0.65 ± 0.01 cm d⁻¹; n = 26; P = 8.3E-06 by two-tailed Wilcoxon rank-sum test) and lifespan (27.6 ± 0.3 d; n = 26; P = 1.1E-02 by two-tailed Wilcoxon rank-sum test) were slightly but significantly increased by +10% and +13%, respectively (Fig. 3c,d and Table 3). These changes were, however, not correlated with a discernible increase in supercomplexes as assayed by BN-PAGE, thereby suggesting that PaRCF1 alone is not sufficient to markedly elevate formation of mtRSCs (Supplementary Fig. S13 and Supplementary Table S1).

Discussion

The mitochondrial protein RCF1 has recently been identified in three independent studies as a novel stabilizing component of the III₂IV₂ supercomplex in S. cerevisiae. It was further demonstrated to preferentially interact with cytochrome c oxidase and to be important for maintaining activity of this complex. In addition, ScRCF1 is also able to independently associate with complex III^18,19,20,21. Despite these insights, the detailed nature and function of ScRCF1 are not entirely clear and the authors’ interpretations are somewhat conflicting. Based on the observation that ScRCF1 maintains association with complex III subunits even in absence of assembled complex IV, one study concluded that ScRCF1 is a true supercomplex assembly factor and not a subunit of complex IV¹⁸. In contrast, Vukotic and colleagues described ScRCF1 as more likely being a new subunit of at least one particular complex IV isoform in which it mediates contact with complex III²⁰. In yet another study, ScRCF1 was recognized as a possible cytochrome c oxidase assembly and regulatory factor but it was also noted that the ability of ScRCF1 to reliably interact with complex III is unique for a potential complex IV component¹⁹.

In our study, we show that RCF1 in P. anserina is absolutely crucial for the stability of complex IV and that PaRCF1 ablation leads to a strong reduction of supercomplex abundance. While it is not yet clear whether PaRCF1 directly interacts with supercomplexes to induce their formation, its impact on overall ETC organization is even more pronounced than what has been observed for RCF1 in S. cerevisiae^18,19,20 or the mammalian RCF1 homolog HIG2A in mice¹⁸. In contrast, deletion of the gene coding for the P. anserina RCF2 homologue led to no pronounced phenotype and did not measurably affect complex IV integrity or ETC organization. While intermediate activation of the alternative respiratory pathway in ΔPaRcf2 clearly argues for a role of PaRCF2 in maintaining standard respiration, it appears questionable whether PaRCF2 is indeed a functional homologue of ScRCF2, which was demonstrated to have partially overlapping functions with ScRCF1^19,20. Variations regarding the relative importance of RCF1 or RCF2 in different model organisms are likely explained by varying ETC compositions, with the most obvious deviation being the complete lack of complex I in S. cerevisiae^37,38. In mammals, which do not possess a RCF2 homologue, complex I has been described as being stabilized by supercomplex formation with complexes III and IV and at the same time to provide a scaffold for efficient respirasome assembly³⁹. There is convincing evidence that in P. anserina the stability of complex I is not dependent on complexes III and/or IV, possibly due to such specific features as presence of the AOX and complex I dimerization²⁷. It can be speculated that complex III and IV interaction in the context of supercomplex formation in turn is less reliant on complex I and mainly dependent on the presence of other factors such as PaRCF1.

Probably the most striking change in the ETC of ΔPaRcf1 is the appearance of a faster migrating complex IV variant (IV_B) which is found almost exclusively instead of fully assembled complex IV. While a comparable phenomenon has so far not been observed in S. cerevisiae, Chen and colleagues report a similar yet less pronounced increase in incomplete complex IV after knockdown of mammalian HIG2A in C2C12 mouse myoblast cells¹⁸. The appearance of the IV_B variant seems to be highly specific for absence of PaRCF1, as it has not been detected nearly as prominently or at all in other P. anserina ETC mutants impaired in supercomplex formation^26,27.

In light of these insights, it is logical to conclude that PaRCF1 is a subunit or a specific regulatory factor of a particular complex IV variant, whose assembly likely precedes and in fact appears to be a prerequisite for formation of the I₁III₂IV_1-2 supercomplexes. Thus, decreased formation of supercomplexes in ΔPaRcf1 could well be a direct consequence of incorrect complex IV assembly. To further the understanding of supercomplex assembly and regulation, it arises as an important future task to address the specialized role and heterogeneous nature of distinct ETC complex variants in different organisms, especially regarding their importance for higher order organization of the respiratory chain.

The majority of studies concerning mitochondrial respiratory supercomplexes have focused on their biochemical, structural and kinetic properties. Consequently, the contribution of mtRSCs to mitochondrial function and organismal integrity is as yet not well understood. One of the first hints at the relevance of mtRSCs in vivo has come from the insight that human Barth syndrome, a hereditary cardiomyopathy occurring exclusively in males, is linked to a destabilization of supercomplexes caused by abnormal mitochondrial cardiolipin^22,23. In addition, a recent study in mice demonstrated the necessity of a supercomplex assembly factor for the regulation of energy metabolism in muscle⁴⁰. To a lesser extent mtRSCs have also been implicated in more complex phenomena such as cancer progression, neurodegeneration and ageing, though causative evidence and understanding of the underlying molecular pathways is still largely missing⁴¹.

Our results clearly demonstrate that RCF1-dependent stability of complex IV and presence of associated supercomplexes in P. anserina is important for mitochondrial function and organismal integrity. On a physiological level, in good agreement with our biochemical observations, the PaRcf1 deletion strain displays reduced complex IV-dependent respiration and activation of the alternative respiratory pathway. Other P. anserina mutants lacking I₁III₂IV_1–2 supercomplexes and respiring primarily via the AOX, owing to defects in complex III and/or complex IV, very consistently display prolonged lifespans^25,27,28,29. In striking contrast to these previous observations, not only are vital functions and superoxide resistance of the PaRcf1 deletion strain negatively affected but its lifespan is also markedly reduced by nearly 50%. On a molecular level, this adverse overall impact is correlated with increased oxidative damage of mitochondrial proteins. This is a further distinguishing feature of ΔPaRcf1, as a switch to AOX-dependent respiration in P. anserina mutants was generally found to result in a decreased ROS burden^25,28. A likely explanation for these findings, aside from an increase in ROS production itself, is the bold reduction of several ROS scavenging and protein quality control components in mitochondria of ΔPaRcf1. The marked reduction of PaSOD3 protein abundance in mitochondria of the PaRcf1 deletion strain seems to be of particular significance because, quite unexpectedly, PaSOD3 activity in ΔPaRcf1 still appears almost identical to that in the wild type. Together with the observation that ΔPaRcf1 is barely able to survive any additional paraquat-induced oxidative stress, this strongly suggests that PaSOD3 in ΔPaRcf1 is working at the limits of its capacity. Interestingly, deletion of S. cerevisiae Rcf1 increased mitochondrial SOD abundance¹⁸. The inability of ΔPaRcf1 to likewise upregulate its mitochondrial SOD in response to elevated endogenous oxidative stress underscores the dramatic effect on respiratory chain integrity and activity following ablation of PaRCF1 and likely reflects impairment of mitochondrial protein import and/or cytoplasmic protein synthesis due to reduced availability of ATP.

Though additional studies are necessary to address these phenomena in detail, it can already be inferred that they must be linked to conditions or properties only present in ΔPaRcf1 and not in other P. anserina ETC mutants. The aforementioned unprecedented and near exclusive emergence of a faster migrating complex IV variant in absence of PaRCF1 meets this criterion. Of note, Vukotic and colleagues proposed that a specific complex IV variant, absent in mitochondria of the S. cerevisiae Rcf1 deletion strain, serves to protect the ETC from excess ROS generation and that lack of ScRCF1 might thus lead to malfunction and ROS production in a catalytic manner²⁰. Despite several crucial differences in the ETC of S. cerevisiae and P. anserina, our results are readily compatible with this model. Beyond that, we identified a novel connection between destabilization of supercomplexes, impaired mitochondrial function and adverse effects on health and lifespan in a simple eukaryotic model organism. As the overall respiratory chain composition of P. anserina is comparable to that of mammals, it will be of great interest to investigate whether similar relationships exist in them as well.

Methods

P. anserina strains and cultivation

In the present study, the P. anserina wild-type strain ‘s’⁴² and newly generated PaRcf1 and PaRcf2 deletion strains (ΔPaRcf1 and ΔPaRcf2) as well as a complemented PaRcf1 deletion strain (ΔPaRcf1/PaRcf1-6xHis) and a strain overexpressing PaRcf1-6xHis in the wild-type background (PaRcf1-6xHis_OEx) were used. All mutant strains are in the genetic background of the wild-type strain ‘s’. Strains were grown on standard cornmeal agar (BMM) at 27 °C under constant light⁴³.

Cloning procedures and generation of P. anserina mutants

Deletion of PaRcf1 and PaRcf2 was performed with the method developed by El-Khoury and colleagues using the plasmid pKO7^30,44. Briefly, approximately 1 kbp long fragments corresponding to the 5′ and 3′ regions of the respective gene of interest were amplified by PCR using sequence specific oligonucleotides with appropriate restriction site overhangs (PaRcf1 5′ region with NotI-PaRcf1_for3: 5′-ATGCGGCCGCCAATAGTGGCTGGGATTT-3′ and BcuI-PaRcf1_rev4: 5′-GCGCACTAGTCTGATCCAGGGAAGATCG-3′; PaRcf1 3′ region with ClaI-PaRcf1_for5: 5′-CGATCGATAAGTGGATGGCTGTAACG-3′ and XhoI-PaRcf1_rev6: 5′-ATCTCGAGCGCAAGCTCCGATTACTG-3′; PaRcf2 5′ region with BcuI-PaRcf2_for3: 5′-GCACTAGTTGGACCAGTCCTGTTGAG-3′ and PstI-PaRcf2_rev4: 5′-ATCTGCAGTGGGTGGAGACTGAAGAG-3′; PaRcf2 3′ region with ClaI-PaRcf2_for5: 5′-GCATCGATTCACGACACGATCTATCC-3′ and XhoI-PaRcf2_rev6: 5′-ATCTCGAGGCTGCTGAATCTGGTGAC-3′; restriction sites underlined) and then cloned into the plasmid pKO7 to flank a hygromycin B resistance gene. The resulting gene specific deletion vectors were used to transform P. anserina spheroblasts of the phleomycin resistant ΔPaKu70 strain. Transformants were then selected by their hygromycin B resistance and crossed with the wild type to reintroduce the PaKu70 gene. Offspring of these crosses were selected by their hygromycin B resistance and phleomycin sensitivity and further verified by Southern blot analysis.

For complementation of the PaRcf1 deletion strain with C-terminally 6xHis-tagged PaRCF1, ΔPaRcf1 spheroblasts were transformed with the plasmid pKO6-PaRcf1-6xHis containing a phleomycin resistance gene in the pKO6 vector backbone⁴⁵ and the full-length PaRcf1 gene under control of its native promotor and terminator, with a 6xHis-tag coding sequence added before the gene’s stop codon. Transformants were selected for phleomycin resistance and verified by Southern blot analysis. Strains with a single integration of pKO6-PaRcf1-6xHis were termed ΔPaRcf1/PaRcf1-6xHis. To construct the vector, the PaRcf1 promotor (~1 kbp of the gene’s upstream region), gene and terminator (~500 bp of the gene’s downstream region) were amplified from genomic wild type DNA by PCR using the oligonucleotides EcoRI-PaRcf1_A (5′-TAGAATTCCGTGTCCGCCCATTCTCG-3′) and SpeI-PaRcf1_B (5′-ATACTAGTCCCACCACCGCAACCTAC-3′), introducing EcoRI and SpeI restriction sites (underlined). The amplicon was cloned into the pKO6 backbone (EcoRI/SpeI digested) to obtain the plasmid pKO6-PaRcf1. Using pKO6-PaRcf1 as a template, a 5′ fragment containing a portion of the PaRcf1 promotor including a PstI restriction site, the full length gene sequence and part of the 6xHis-tag coding sequence was amplified by PCR using the oligonucleotides PaRcf1_Hisfor1 (5′-GTCGTTGAAGTCGCAAGAAG-3′) and PaRcf1_Hisrev2 (5′-GGTGGTGATGGTTCTTCGGGTCTTCTG-3′). A 3′ fragment containing the remaining 6xHis-tag coding sequence and the PaRcf1 terminator including a BcuI restriction site was amplified by PCR using the oligonucleotides PaRCF1_Hisfor3 (5′-ATCACCATTAAGGGTGAAGTGGATGGC-3′) and PaRcf1_Hisrev4 (5′-GCCGCTCTAGAACTAGTCC-3′). The fragments were then cloned into the pKO6-PaRcf1 backbone (PstI/BcuI digested) to obtain pKO6-PaRcf1-6xHis.

To generate a PaRcf1-6xHis overexpressing strain, wild-type spheroblasts were transformed with the newly constructed plasmid pExMtterhph-PaRcf1-6xHis-OEx containing a hygromycin B resistance gene in the pExMtterhph vector backbone⁴⁶ and the full-length PaRcf1 gene under control of the strong constitutive metallothionein promoter and the metallothionein terminator, with a 6xHis-tag coding sequence added before the gene’s stop codon. Transformants were selected for hygromycin B resistance and verified by Southern blot analysis. Strains with a single integration of pExMtterhph-PaRcf1-6xHis-OEx were termed PaRcf1-6xHis_OEx. To construct the vector, the PaRcf1 gene including the 6xHis-tag coding sequence was amplified by PCR using the oligonucleotides BglII-PaRcf1_for (5′-TAAGATCTATGTCGAACGGACCCCTCTC-3′) and XbaI-6xHis_rev (5′-GCTCTAGATTAATGGTGATGGTGGTGATG-3′) with pKO6-PaRcf1-6xHis as a template, introducing BglII and XbaI restriction sites (underlined). The amplicon was cloned into the pExMtterhph backbone (BamHI/XbaI digested) to obtain pExMtterhph-PaRcf1-6xHis-OEx.

Transformation of P. anserina spheroblasts

The respective strain to be transformed was grown on BMM at 27 °C under constant light for 3 days and subsequently under the same conditions in liquid complete medium (CM) for 2 days (CM medium: 1 g/l KH₂PO₄, 0.5 g/l KCl, 0.5 g/l MgSO₄ × 7 H₂O, 10 g/l glucose, 3.7 g/l NH₄Cl, 2 g/l tryptone, 2 g/l yeast extract and 1 g/l ZnSO₄, FeCl₂ and MnCl₂; pH 6.5). 20 g of the resulting mycelium was washed with TPS buffer (5 mM Na₂HPO₄, 45 mM KH₂PO₄, 0.8 M sucrose; pH 5.5) and TPS buffer containing 20 mg/ml ‘Glucanex’ (Novozymes) was added to a final volume of 100 ml. After chopping the mixture in a ‘Waring Blendor’ the resulting suspension was incubated for 1.5 h at 35 °C. Following filtration through gauze and glass wool, the suspension was centrifuged for 10 min at 4.000 rpm to pelletise the spheroplasts and the pellet was washed three times with TPS buffer.

To regenerate spheroplasts, the pellet was recovered in TPS buffer and the spheroplasts were plated on regeneration agar (3.7 g/l NH₄Cl, 2 g/l tryptone, 1 g/l casamino acids, 1 g/l yeast extract, 10 g/l glucose, 342.3 g/l sucrose, 1.5 g/l KH₂PO₄, 0.5 g/l KCl, 0.54 g/l MgSO₄ and 1 mg/l MnSO₄ × 1 H₂O, FeSO₄ × 7 H₂O, CuSO₄ × 5 H₂O and ZnSO₄ × 7 H₂O) containing 100 μg/ml hygromycin B. After 7–10 days of growth, mycelia of developing cultures were transferred to BMM agar plates.

Integrative transformation of P. anserina spheroplasts was performed as described previously⁴⁷.

Lifespan determination

To determine the lifespan of the strains used in this study, monokaryotic ascospores were isolated from independent crosses of the respective strains (in the case of the PaRcf1 deletion strain from a cross of wild type with ΔPaRcf1) and germinated for 3 days at 27 °C in the dark on BMM supplemented with 60 mM ammonium acetate. After germination, pieces of the resulting 3 day old mycelia were placed on M2 agar race tubes (M2 medium: 0.25 g/l KH₂PO₄, 0.3 g/l K₂HPO₄, 0.25 g/l MgSO₄ × 7 H₂O, 0.5 g/l urea and 10 g/l yellow dextrin. Addition of 2.5 μg/l biotin, 50 μg/l thiamine, 5 mg/l citric acid × 1 H₂O, 5 mg/l ZnSO₄ × 7 H₂O, 1 mg/l Fe(NH₄)₂(SO₄)₂ × 6 H₂O, 2.5 mg/l CuSO₄ × 5 H₂O, 25 μg/l MnSO₄ × 1 H₂O, 50 μg/l NaMoO₄ × 2 H₂O and 50 μg/l H₃BO₄ after sterilization of the basal medium) and incubated at 27 °C under constant light. The period of linear growth was recorded as lifespan in days. Growth rate was measured as growth of the mycelia in centimetres per day.

Measurement of growth rate under stress conditions

To assess the susceptibility of the strains used in this study to paraquat-induced oxidative stress, monokaryotic ascospores were germinated as described above. After germination, pieces of the resulting 3 d old mycelia were placed on agar plates containing M2 medium supplemented with different concentrations of paraquat (0, 80, 160 or 320 μM) and incubated at 27 °C under constant light. Growth was recorded for 4 days and growth rate was expressed as growth of the mycelia in centimetres per day.

Fertility analysis

Assessment of female fertility was essentially performed as described previously⁴⁸. Isolates of wild type, ΔPaRcf1 and ΔPaRcf2, in each case originating from monokaryotic ascospores, were grown on agar plates containing M2 medium at 27 °C under constant light for 13 days. Following spermatization at day 13, all plates were incubated for an additional three days at 27 °C, after which the total number of perithecia developing on each plate was counted. The mean number of perithecia developing per plate overgrown with the wild-type strain ‘s’ at 27 °C was defined as 1.

Oxygen consumption measurement

To measure complex IV- and AOX-dependent oxygen consumption of wild type, ΔPaRcf1 and ΔPaRcf2, monokaryotic ascospores of each strain were germinated as described above. After germination, pieces of the resulting 3 d old mycelia were grown for 2 days on M2 medium at 27 °C under constant light and subsequently under the same conditions in liquid CM for 3 days. Small pieces of mycelium (dry weight 2 to 10 mg) were then transferred into the ‘OROBOROS Oxygraph-2k’ (OROBOROS INSTRUMENTS) high-resolution respirometer and oxygen consumption was measured in liquid CM medium according to the manufacturer’s instructions. To inhibit respiration via complex IV, KCN was added to a final concentration of 1 mM. Respiration via AOX was inhibited by adding salicylhydroxamic acid (SHAM) to a final concentration of 4 mM. Absolute oxygen consumption was measured as pmol oxygen consumed per second and milligram dry weight mycelium. To express relative oxygen consumption after addition of specific respiratory inhibitors, absolute oxygen consumption of the respective strain (wild type, ΔPaRcf1 or ΔPaRcf2) in the presence of KCN or SHAM was normalized to its total absolute oxygen consumption with no added inhibitors.

Southern blot analysis

Total DNA of P. anserina was isolated with a well-established method for rapid extraction of nucleic acids from filamentous fungi⁴⁹. DNA digestion, gel electrophoresis and Southern blotting were performed according to standard protocols. For Southern blot hybridization and detection, Digoxigenin-labeled hybridization probes (‘DIG DNA Labeling and Detection Kit’, Roche Applied Science) were used according to the manufacturer’s instructions.

The PaRcf1-specific hybridization probe was amplified by PCR using the oligonucleotides PaRcf1_A2 (5′-AGGAACCGCTCGTCCCAATC-3′) and PaRcf1_B2 (5′-CCTTGCCTGAGCAGCAACAC-3′) and corresponded to 371 nucleotides in exon 2 of PaRcf1. The PaRcf2-specific hybridization probe was amplified by PCR using the oligonucleotides PaRcf2_for1 (5′-AAGACGCCCACTTCAAGG-3′) and PaRcf2_rev2 (5′-TGGCTTCCGCTCAGATAC-3′) and corresponded to 408 nucleotides in exon 1 of PaRcf2. The hph-specific hybridization probe corresponded to the 727 bp ClaI-NcoI-fragment of the plasmid pKO7⁴⁴. As a hybridization probe specific for the phleomycin resistance gene (ble), the 1293 bp BamHI-fragment of the plasmid pKO3⁵⁰ was used.

Western blot analysis

Mitochondrial protein extracts from P. anserina strains were isolated according to a previously developed procedure²⁵ and further purified by discontinuous sucrose gradient (20-36–50%) ultracentrifugation⁴⁴. Mitochondrial protein extracts for ‘OxyBlot’ analysis were isolated in the presence of 50 mM dithiothreitol and treated with the ‘OxyBlot™ Protein Oxydation Detection Kit’ (Merck Millipore) according to the manufacturer’s instructions. Separation of proteins by SDS-PAGE and subsequent transfer of proteins to PVDF membranes (Immobilon-FL, Millipore) were performed following standard protocols. Blocking and antibody incubation of blotted PVDF membranes were performed according to the Odyssey ‘Western Blot Analysis’ handbook (LI-COR).

Primary antibodies were raised against a PaCLPP (UniProt: B2B591) specific synthetic peptide ([Ac]-CGTMLSADAKEGKH-[OH]; NEP) corresponding to AA 242-254 (antibody dilution: 1:400), a PaLON (UniProt: B2AZ54) specific synthetic peptide ([H]-CDKIGRGYQGDPS-[OH]; Sigma) corresponding to AA 677-688 (antibody dilution: 1:1,500), a PaPRX (UniProt: B2AKR1) specific synthetic peptide ([Ac]-LHESSPGNKVNLADC-[NH2]; NEP) corresponding to AA 43-56 (antibody dilution: 1:2,000) and the PaPORIN (UniProt: B2B736) full-length protein (NEP; antibody dilution: 1:5,000). A human aconitase 2 antibody (abcam, product code: ab83528) used to detect P. anserina mitochondrial aconitase (UniProt: B2VLF5) was raised against a HsACO2 (UniProt: Q99798) specific recombinant fragment corresponding to AA 648-697 (antibody concentration: 1 μg/ml). A Sauromatum guttatum AOX antibody (Agrisera, product code: AS10 699) used to detect P. anserina AOX (UniProt: B2ACQ1) was raised against the SgAOX (UniProt: P22185) full-length protein (antibody dilution: 1:100). For detection of P. anserina HSP60 (UniProt: B2B270) an antibody (Stressgen, product code: SPA-807) raised against human full-length HSP60 (UniProt: P10809) was used (antibody concentration: 0.25 μg/ml). For detection of P. anserina SOD3 (UniProt: B2B5F1) an antibody (Stressgen, product code: SOD-111) raised against full-length rat SOD2 (UniProt: P07895) was used (antibody dilution: 1:2,000). Detection of PaRCF1 (UniProt: Q875C2) with a C-terminal 6xHis-tag was performed using a 6xHis-tag antibody (abcam, product code: ab9136; antibody dilution: 1:1,000). The S. cerevisiae COX2 (UniProt: P00410) antibody (antibody dilution: 1:5,000) used to detect PaCOX2 (UniProt: P20682) was a kind gift from Prof. T. Langer (Institute for Genetics, University of Cologne, Germany). Carbonyl groups of oxidized proteins derivatized to 2,4-dinitrophenylhydrazone (DNP-hydrazone) were detected with the DNP-specific antibody (antibody dilution: 1:100) provided with the ‘OxyBlot™ Protein Oxydation Detection Kit’ (Merck Millipore).

In all analyses, secondary antibodies conjugated with the infrared dyes IRDye 800CW or IRDye 680CW (LI-COR) were used (antibody dilution: 1:15,000–20,000). The ‘Odyssey Infrared Imaging System’ (LI-COR) was used for detection of western blots and densitometric quantification was performed with the image processing and analysis software ImageJ according to the developer’s documentation.

BN-PAGE and complex IV ‘in-gel’ activity assay

BN-PAGE was performed according to the protocol described in detail by Wittig and colleagues⁵¹. For preparation of each sample, 100 μg of mitochondrial protein extracts were solubilized using a digitonin/protein ratio of 3:1 (w/w). Linear gradient gels (4–13%) overlaid with 3.5% stacking gels were used for separation of the solubilised samples. Respiratory chain components were then visualized by Coomassie blue staining and assigned as described previously²⁶. To measure complex IV ‘in-gel’ activity, Coomassie blue staining was omitted and the gel was incubated in 50 mM phosphate buffer (pH 7.4) containing 1 mg/ml 3,3′-diaminobenzidine, 24 U/ml catalase, 1 mg/ml cytochrome c and 75 mg/ml sucrose⁵². Densitometric quantification was performed as described above.

Statistical analysis

For statistical analysis of BN-PAGE, complex ‘in-gel’ activity, western blot and OxyBlot data as well as oxygen consumption measurements, two-tailed Student’s t-test was used. For statistical analysis of lifespan and growth rate, two-tailed Wilcoxon rank-sum test was used. If not explicitly stated otherwise, the respective samples were compared to the appropriate wild-type sample. P-values < 0.05 were considered statistically significant.

Additional Information

How to cite this article: Fischer, F. et al. RCF1-dependent respiratory supercomplexes are integral for lifespan-maintenance in a fungal ageing model. Sci. Rep. 5, 12697; doi: 10.1038/srep12697 (2015).

References

Tatsuta, T. & Langer, T. Quality control of mitochondria: protection against neurodegeneration and ageing. EMBO J. 27, 306–314 (2008).
Article CAS PubMed PubMed Central Google Scholar
Baker, B. M. & Haynes, C. M. Mitochondrial protein quality control during biogenesis and aging. Trends Biochem. Sci. 36, 254–261 (2011).
Article CAS PubMed Google Scholar
Coskun, P. et al. A mitochondrial etiology of Alzheimer and Parkinson disease. Biochim. Biophys. Acta 1820, 553–564 (2012).
Article CAS PubMed Google Scholar
Fischer, F., Hamann, A. & Osiewacz, H. D. Mitochondrial quality control: an integrated network of pathways. Trends Biochem. Sci. 37, 284–292 (2012).
Article CAS PubMed Google Scholar
Vafai, S. B. & Mootha, V. K. Mitochondrial disorders as windows into an ancient organelle. Nature 491, 374–383 (2012).
Article ADS CAS PubMed Google Scholar
Harman, D. The biologic clock: the mitochondria? J. Am. Geriatr. Soc. 20, 145–147 (1972).
Article CAS PubMed Google Scholar
Brand, M. D. et al. Mitochondrial superoxide: production, biological effects and activation of uncoupling proteins. Free Radic. Biol. Med. 37, 755–767 (2004).
Article CAS PubMed Google Scholar
Lapointe, J. & Hekimi, S. When a theory of aging ages badly. Cell Mol. Life Sci. 67, 1–8 (2010).
Article CAS PubMed PubMed Central Google Scholar
Stuart, J. A., Maddalena, L. A., Merilovich, M. & Robb, E. L. A midlife crisis for the mitochondrial free radical theory of aging. Longev. Healthspan. 3, 4 (2014).
Article PubMed PubMed Central Google Scholar
D’Autreaux, B. & Toledano, M. B. ROS as signalling molecules: mechanisms that generate specificity in ROS homeostasis. Nat. Rev. Mol. Cell Biol. 8, 813–824 (2007).
Article PubMed CAS Google Scholar
Hamanaka, R. B. et al. Mitochondrial reactive oxygen species promote epidermal differentiation and hair follicle development. Sci. Signal. 6, ra8 (2013).
Article PubMed PubMed Central CAS Google Scholar
Schägger, H. & Pfeiffer, K. Supercomplexes in the respiratory chains of yeast and mammalian mitochondria. EMBO J. 19, 1777–1783 (2000).
Article PubMed PubMed Central Google Scholar
Acin-Perez, R., Fernandez-Silva, P., Peleato, M. L., Perez-Martos, A. & Enriquez, J. A. Respiratory active mitochondrial supercomplexes. Mol. Cell 32, 529–539 (2008).
Article CAS PubMed Google Scholar
Acin-Perez, R. & Enriquez, J. A. The function of the respiratory supercomplexes: the plasticity model. Biochim. Biophys. Acta 1837, 444–450 (2014).
Article CAS PubMed Google Scholar
Boekema, E. J. & Braun, H. P. Supramolecular structure of the mitochondrial oxidative phosphorylation system. J. Biol. Chem. 282, 1–4 (2007).
Article CAS PubMed Google Scholar
Vartak, R., Porras, C. A. & Bai, Y. Respiratory supercomplexes: structure, function and assembly. Protein Cell 4, 582–590 (2013).
Article CAS PubMed PubMed Central Google Scholar
Genova, M. L. & Lenaz, G. Functional role of mitochondrial respiratory supercomplexes. Biochim. Biophys. Acta 1837, 427–443 (2014).
Article CAS PubMed Google Scholar
Chen, Y. C. et al. Identification of a protein mediating respiratory supercomplex stability. Cell Metab 15, 348–360 (2012).
Article CAS PubMed PubMed Central Google Scholar
Strogolova, V., Furness, A., Robb-McGrath, M., Garlich, J. & Stuart, R. A. Rcf1 and Rcf2, members of the hypoxia-induced gene 1 protein family, are critical components of the mitochondrial cytochrome bc1-cytochrome c oxidase supercomplex. Mol. Cell Biol. 32, 1363–1373 (2012).
Article CAS PubMed PubMed Central Google Scholar
Vukotic, M. et al. Rcf1 mediates cytochrome oxidase assembly and respirasome formation, revealing heterogeneity of the enzyme complex. Cell Metab 15, 336–347 (2012).
Article CAS PubMed Google Scholar
Shoubridge, E. A. Supersizing the mitochondrial respiratory chain. Cell Metab 15, 271–272 (2012).
Article CAS PubMed Google Scholar
McKenzie, M., Lazarou, M., Thorburn, D. R. & Ryan, M. T. Mitochondrial respiratory chain supercomplexes are destabilized in Barth Syndrome patients. J. Mol. Biol. 361, 462–469 (2006).
Article CAS PubMed Google Scholar
Gonzalvez, F. et al. Barth syndrome: cellular compensation of mitochondrial dysfunction and apoptosis inhibition due to changes in cardiolipin remodeling linked to tafazzin (TAZ) gene mutation. Biochim. Biophys. Acta 1832, 1194–1206 (2013).
Article CAS PubMed Google Scholar
Osiewacz, H. D. Mitochondrial quality control in aging and lifespan control of the fungal aging model Podospora anserina. Biochem. Soc. Trans. 39, 1488–1492 (2011).
Article CAS PubMed Google Scholar
Gredilla, R., Grief, J. & Osiewacz, H. D. Mitochondrial free radical generation and lifespan control in the fungal aging model Podospora anserina. Exp. Gerontol. 41, 439–447 (2006).
Article CAS PubMed Google Scholar
Krause, F. et al. Supramolecular organization of cytochrome c oxidase- and alternative oxidase-dependent respiratory chains in the filamentous fungus Podospora anserina. J. Biol. Chem. 279, 26453–26461 (2004).
Article CAS PubMed Google Scholar
Maas, M. F., Krause, F., Dencher, N. A. & Sainsard-Chanet, A. Respiratory complexes III and IV are not essential for the assembly/stability of complex I in fungi. J. Mol. Biol. 387, 259–269 (2009).
Article CAS PubMed Google Scholar
Dufour, E., Boulay, J., Rincheval, V. & Sainsard-Chanet, A. A causal link between respiration and senescence in Podospora anserina. Proc. Natl. Acad. Sci. USA 97, 4138–4143 (2000).
Article ADS CAS PubMed PubMed Central Google Scholar
Stumpferl, S. W., Stephan, O. & Osiewacz, H. D. Impact of a disruption of a pathway delivering copper to mitochondria on Podospora anserina metabolism and life span. Eukaryot. Cell 3, 200–211 (2004).
Article CAS PubMed PubMed Central Google Scholar
El-Khoury, R. et al. Gene deletion and allelic replacement in the filamentous fungus Podospora anserina. Curr. Genet. 53, 249–258 (2008).
Article CAS PubMed Google Scholar
Borghouts, C., Werner, A., Elthon, T. & Osiewacz, H. D. Copper-modulated gene expression and senescence in the filamentous fungus Podospora anserina. Mol. Cell Biol. 21, 390–399 (2001).
Article CAS PubMed PubMed Central Google Scholar
Cocheme, H. M. & Murphy, M. P. Complex I is the major site of mitochondrial superoxide production by paraquat. J. Biol. Chem. 283, 1786–1798 (2008).
Article CAS PubMed Google Scholar
Lee, S. J., Hwang, A. B. & Kenyon, C. Inhibition of respiration extends C. elegans life span via reactive oxygen species that increase HIF-1 activity. Curr. Biol. 20, 2131–2136 (2010).
Article CAS PubMed PubMed Central Google Scholar
Yang, W. & Hekimi, S. A mitochondrial superoxide signal triggers increased longevity in Caenorhabditis elegans. PLoS. Biol. 8, e1000556 (2010).
Article PubMed PubMed Central CAS Google Scholar
Wiemer, M. & Osiewacz, H. D. Effect of paraquat-induced oxidative stress on gene expression and aging of the filamentous ascomycete Podospora anserina. Microb. Cell 1, 225–240 (2014).
Article CAS PubMed PubMed Central Google Scholar
Denko, N. et al. Epigenetic regulation of gene expression in cervical cancer cells by the tumor microenvironment. Clin. Cancer Res. 6, 480–487 (2000).
CAS PubMed Google Scholar
Nosek, J. & Fukuhara, H. NADH dehydrogenase subunit genes in the mitochondrial DNA of yeasts. J. Bacteriol. 176, 5622–5630 (1994).
Article CAS PubMed PubMed Central Google Scholar
Joseph-Horne, T., Hollomon, D. W. & Wood, P. M. Fungal respiration: a fusion of standard and alternative components. Biochim. Biophys. Acta 1504, 179–195 (2001).
Article CAS PubMed Google Scholar
Moreno-Lastres, D. et al. Mitochondrial complex I plays an essential role in human respirasome assembly. Cell Metab 15, 324–335 (2012).
Article CAS PubMed PubMed Central Google Scholar
Ikeda, K., Shiba, S., Horie-Inoue, K., Shimokata, K. & Inoue, S. A stabilizing factor for mitochondrial respiratory supercomplex assembly regulates energy metabolism in muscle. Nat. Commun. 4, 2147 (2013).
Article ADS PubMed CAS Google Scholar
Porras, C. A. & Bai, Y. Respiratory supercomplexes: plasticity and implications. Front Biosci. (Landmark. Ed) 20, 621–634 (2015).
Article CAS Google Scholar
Rizet, G. [Impossibility of obtaining uninterrupted and unlimited multiplication of the ascomycete Podospora anserina]. C. R. Hebd. Seances Acad. Sci. 237, 838–840 (1953).
CAS PubMed Google Scholar
Esser, K. Podospora anserina in Handbook of Genetics (ed. King, R. C. ) 531–551 (Plenum Press, 1974).
Kunstmann, B. & Osiewacz, H. D. The S-adenosylmethionine dependent O-methyltransferase PaMTH1: a longevity assurance factor protecting Podospora anserina against oxidative stress. Aging (Albany. NY) 1, 328–334 (2009).
Article CAS Google Scholar
Knuppertz, L., Hamann, A., Pampaloni, F., Stelzer, E. & Osiewacz, H. D. Identification of autophagy as a longevity-assurance mechanism in the aging model Podospora anserina. Autophagy. 10, 822–834 (2014).
Article CAS PubMed PubMed Central Google Scholar
Luce, K. & Osiewacz, H. D. Increasing organismal healthspan by enhancing mitochondrial protein quality control. Nat. Cell Biol. 11, 852–858 (2009).
Article CAS PubMed Google Scholar
Osiewacz, H. D., Skaletz, A. & Esser, K. Integrative transformation of the ascomycete Podospora anserina: identification of the mating-type locus on chromosome VII of electrophoretically separated chromosomes. Appl. Microbiol. Biotechnol. 35, 38–45 (1991).
Article CAS PubMed Google Scholar
Weil, A. et al. Unmasking a temperature-dependent effect of the P. anserina i-AAA protease on aging and development. Cell Cycle 10, 4280–4290 (2011).
Article CAS PubMed PubMed Central Google Scholar
Lecellier, G. & Silar, P. Rapid methods for nucleic acids extraction from Petri dish-grown mycelia. Curr. Genet. 25, 122–123 (1994).
Article CAS PubMed Google Scholar
Hamann, A., Krause, K., Werner, A. & Osiewacz, H. D. A two-step protocol for efficient deletion of genes in the filamentous ascomycete Podospora anserina. Curr. Genet. 48, 270–275 (2005).
Article CAS PubMed Google Scholar
Wittig, I., Braun, H. P. & Schagger, H. Blue native PAGE. Nat. Protoc. 1, 418–428 (2006).
Article CAS PubMed Google Scholar
Jung, C., Higgins, C. M. & Xu, Z. Measuring the quantity and activity of mitochondrial electron transport chain complexes in tissues of central nervous system using blue native polyacrylamide gel electrophoresis. Anal. Biochem. 286, 214–223 (2000).
Article CAS PubMed Google Scholar

Download references

Acknowledgements

This work was supported by a generous grant of the Hessian Ministry of Science and Art to H.D.O. within the framework of the LOEWE research focus ‘Integrative Fungal Research’. We thank Prof. T. Langer (Institute for Genetics, University of Cologne, Germany) for the kind gift of S. cerevisiae COX2 antibody, Prof. A. Sainsard-Chanet (Centre de Génétique Moléculaire, Gif-sur-Yvette, France) for the P. anserina ΔPaKu70 strain and N. Schäffner for performing additional validating experiments.

Author information

Authors and Affiliations

Johann Wolfgang Goethe University, Faculty for Biosciences & Cluster of Excellence ‘Macromolecular Complexes’ Frankfurt, Institute of Molecular Biosciences, Max-von-Laue-Str. 9, Frankfurt, 60438, Germany
Fabian Fischer, Christodoulos Filippis & Heinz D. Osiewacz

Authors

Fabian Fischer
View author publications
You can also search for this author in PubMed Google Scholar
Christodoulos Filippis
View author publications
You can also search for this author in PubMed Google Scholar
Heinz D. Osiewacz
View author publications
You can also search for this author in PubMed Google Scholar

Contributions

F.F. and H.D.O. designed this study. F.F. and C.F. performed the experiments and analysed the data. F.F. and H.D.O. wrote the manuscript. All authors read and approved the final manuscript.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Electronic supplementary material

Supplementary Information

Rights and permissions

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

Reprints and permissions

About this article

Cite this article

Fischer, F., Filippis, C. & Osiewacz, H. RCF1-dependent respiratory supercomplexes are integral for lifespan-maintenance in a fungal ageing model. Sci Rep 5, 12697 (2015). https://doi.org/10.1038/srep12697

Download citation

Received: 08 April 2015
Accepted: 07 July 2015
Published: 29 July 2015
DOI: https://doi.org/10.1038/srep12697

This article is cited by

Cryo-EM structure and function of S. pombe complex IV with bound respiratory supercomplex factor
- Agnes Moe
- Pia Ädelroth
- Linda Näsvik Öjemyr
Communications Chemistry (2023)
Regulation of cytochrome c oxidase activity by modulation of the catalytic site
- Jacob Schäfer
- Hannah Dawitz
- Peter Brzezinski
Scientific Reports (2018)

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Subjects

Abstract

Similar content being viewed by others

Messenger RNA transport on lysosomal vesicles maintains axonal mitochondrial homeostasis and prevents axonal degeneration

Small extracellular vesicles from young plasma reverse age-related functional declines by improving mitochondrial energy metabolism

A guide to cell death pathways

Introduction

Results

Deletion of PaRcf1 disturbs complex IV and supercomplexes

ETC alterations in ΔPaRcf1 impair mitochondrial integrity

ΔPaRcf1 is unable to maintain a healthy lifespan

Discussion

Methods

P. anserina strains and cultivation

Cloning procedures and generation of P. anserina mutants

Transformation of P. anserina spheroblasts

Lifespan determination

Measurement of growth rate under stress conditions

Fertility analysis

Oxygen consumption measurement

Southern blot analysis

Western blot analysis

BN-PAGE and complex IV ‘in-gel’ activity assay

Statistical analysis

Additional Information

References

Acknowledgements

Author information

Authors and Affiliations

Contributions

Ethics declarations

Competing interests

Electronic supplementary material

Supplementary Information

Rights and permissions

About this article

Cite this article

Share this article

This article is cited by

Cryo-EM structure and function of S. pombe complex IV with bound respiratory supercomplex factor

Regulation of cytochrome c oxidase activity by modulation of the catalytic site

Comments

Search

Quick links