Alcohol sedation in adult Drosophila is regulated by Cysteine proteinase-1 in cortex glia

Lee, Kristen M.; Mathies, Laura D.; Grotewiel, Mike

doi:10.1038/s42003-019-0492-5

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Published: 03 July 2019

Alcohol sedation in adult Drosophila is regulated by Cysteine proteinase-1 in cortex glia

Communications Biology volume 2, Article number: 252 (2019) Cite this article

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Abstract

Although numerous studies have demonstrated that neuronal mechanisms regulate alcohol-related behaviors, very few have investigated the direct role of glia in behavioral responses to alcohol. The results described here begin to fill this gap in the alcohol behavior and gliobiology fields. Since Drosophila exhibit conserved behavioral responses to alcohol and their CNS glia are similar to mammalian CNS glia, we used Drosophila to begin exploring the role of glia in alcohol behavior. We found that knockdown of Cysteine proteinase-1 (Cp1) in glia increased Drosophila alcohol sedation and that this effect was specific to cortex glia and adulthood. These data implicate Cp1 and cortex glia in alcohol-related behaviors. Cortex glia are functionally homologous to mammalian astrocytes and Cp1 is orthologous to mammalian Cathepsin L. Our studies raise the possibility that cathepsins may influence behavioral responses to alcohol in mammals via roles in astrocytes.

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Introduction

Alcohol use disorder, defined as chronic alcohol abuse and dependence (DSM-V)¹, is a major health problem. For example, alcohol abuse is the third leading risk factor for death and disability², excessive alcohol consumption is estimated to be responsible for ~2.5 million preventable deaths worldwide per year, and alcohol abuse costs the United States ~200 billion dollars annually^3,4,5. Thus, there is a pressing need to better understand the mechanisms involved in the development of alcohol use disorder, identify individuals at risk for alcohol use disorder, and ultimately provide improved treatment options for the disorder.

In largely naive alcohol drinkers, the initial level of response to alcohol correlates with their likelihood of becoming alcohol dependent⁶, a phenotype associated with alcohol use disorder¹. For example, men with an initially low sensitivity to alcohol are four times more likely to be an alcoholic by age 30⁶. Therefore, investigating molecular-genetic mechanisms that influence alcohol sensitivity is a potentially promising approach for understanding the molecular underpinnings of alcohol use disorder.

The fruit fly Drosophila melanogaster, the nematode C. elegans and rodents have been used extensively to investigate the genetics of alcohol-related behaviors, including alcohol sedation. Numerous genes involved in alcohol-related behaviors in model organisms have human orthologs that have been implicated in human alcohol abuse, suggesting mechanistic connections between alcohol-related behaviors in model organisms and alcohol abuse in humans^7,8. A majority of these genes are known or predicted to function in neurons⁷, leaving the contribution of glia and glial cell mechanisms to alcohol-related behavior largely unexplored. To the best of our knowledge, only three studies have investigated the direct contribution of glia in alcohol-related behaviors. One study found that activation of calcium signaling in rat nucleus accumbens core astrocytes via DREADDS decreases motivation for alcohol after a 3-week-long alcohol abstenance⁹. Another study found that Drosophila with a mutation in the gene moody, a gene expresssed in surface glia as well as other cell types, have reduced sensitivity to ethanol-induced loss of postural control¹⁰. An additional study in Drosophila found that surface glia also contribute to alcohol tolerance¹¹. Despite these pioneering studies, our understanding of the role of glia in alcohol-related behavior is woefully incomplete.

The Drosophila central nervous system (CNS) is compartmentalized into two gross anatomical regions: an outer cortex (containing neuronal cell bodies) and a more central neuropil (containing neurites and synapses). Like mammals, the Drosophila CNS is composed of both neurons and glia. Drosophila CNS glia are functionally and molecularly similar to mammalian CNS glia^{12,13,14,15,16}. Cortex glia, astrocytes, and ensheathing cells are the main subtypes of CNS glia in adult flies¹². Additionally, perineural and subperineural glia, often referred to as surface glia, surround the entire CNS and compose the blood brain barrier in flies^17,18. Drosophila cortex glia and astrocytes are intimately associated with neurons in the CNS¹⁹. Cortex glia are located in the cortex region of the brain and encapsulate virtually all neuronal cell bodies with fine processes²⁰. A single adult cortex glial cell is thought to be able to encapsulate up to 100 neurons¹⁹. Cortex glia aid in gas exchange, neuronal firing, and nutrient transfer to neurons, similarly to mammalian protoplasmic astrocytes^12,20,21. Cortex glia also exhibit calcium transients near membranes close to neurons, which appear to regulate neuronal cell function²². Physical associations between cortex glia and neurons are essential for normal nervous system function and behavior in Drosophila²³. In contrast to cortex glia, the cell bodies of astrocytes reside at the cortex-neuropil interface and extend processes into the neuropil²⁴. Like mammalian astrocytes, Drosophila astrocytes are important for synapse formation and maintenance, clearing and recycling neurotransmitters from the synapse, and modulating neuronal physiology^25,26. Drosophila astrocytes release gliotransmitters, which are regulated by transient intracellular calcium signaling; this mechanism can directly influence nearby cells and influence behavior^15,27,28. The cell bodies of ensheathing glia are also located at the interface of the brain cortex and neuropil²⁴. Under normal physiological conditions, ensheathing glia encase the entire neuropil region in the CNS and occasionally wrap axonal segments between the neuropil and the periphery²⁹. Ensheathing glia can regulate neuronal excitability by metabolizing glutamate, and disruptions in this function can alter behavior³⁰. Under pathological conditions, these cells extend processes into the neuropil to phagocytize debris^24,31,32. Drosophila surface glia (i.e., subperineural and perineural glia) are less similar to mammalian glia, but they have been associated with alcohol-related behavior in flies^10,11. Subperineural glia mediate most of the blood brain barrier chemoprotective functions, similar to mammalian brain vascular endothelial cells³³. Interestingly, subperineural glia can extend processes, which function at PNS synapses³⁴. As their name implies, perineural glia reside on top of the subperineural glia, and protect against the entrance of larger molecules³⁵. With macrophages, these cells secrete a dense lamella that covers the CNS and peripheral nerves³⁵. Despite being extensively investigated in numerous experimental settings, a role for glia in fly alcohol-related behavior has not been comprehensively explored.

Here, we demonstrate that RNAi-mediated knockdown and rescue of the gene Cysteine proteinase-1 (Cp1) constitutively in all CNS glia regulates alcohol sedation. This behavioral effect appears specific to Cp1 expression in cortex glia, as well as all glia during adulthood. Cp1 is a hydrolase involved in protein degradation that is functionally and structurally homologous to mammalian Cathepsin L³⁶. Our data suggest a previously unidentified role for cortex glia and Cp1 in the adult Drosophila CNS: regulation of sedation in response to acute administration of alcohol.

Results

Identifying glial genes that influence alcohol sedation

To begin exploring the role of central nervous system (CNS) glia in alcohol behavior, we performed a targeted screen in which we compiled genes previously reported to be expressed in glia^13,37,38,39, obtained genetic reagents to manipulate the expression of those genes, and determined whether constitutive or induced overexpression, expression of dominant negatives, or expression of RNAi targeting those genes influenced alcohol sedation. In total, we screened 19 genes by RNAi, nine by overexpression and five by dominant negatives.

One of the genes identified by this targeted screen was Cysteine proteinase-1 (Cp1). Cp1 is known to function in Drosophila midgut, garland cells, salivary glands, macrophages, gonads, and PNS neurons^{36,40,41,42,43} and is expressed in glia¹³, but prior to our results no studies have demonstrated a functional role for Cp1 in glia. Cp1 is the only Drosophila cysteine proteinase that has been described and is functionally and structurally homologous to mammalian Cathepsin L^41,44. Although cysteine proteinases play key roles in the lysosomes of phagocytic cells⁴³ and mammalian Cathepsin L has been associated with multiple diseases, including cancer^45,46, Alzheimer disease⁴⁷, and retinal degeneration⁴¹, no previous studies implicate this family of genes in alcohol-related behavior.

Glial Cp1 regulates the pharmacodynamics of alcohol sedation

Flies with pan-glial Gal4 (repo-Gal4) driven expression of two different Cp1 RNAi transgenes (v13959 and HMS00725, tested individually) had decreased sedation time 50 (ST50) values compared to control flies containing the Gal4 or an RNAi transgene alone (Fig. 1a, b). For reasons that are unclear, constitutive expression of a third RNAi transgene (v110619) in all glia did not consistently alter alcohol sedation (Supplementary Fig. 1a). To determine if Cp1 influenced alcohol metabolism, we measured the internal alcohol levels in these same genotypes after a 30-min alcohol exposure (approximating the ST50). We found no significant difference in the internal alcohol concentrations between flies expressing Cp1 RNAi transgenes in glia compared to controls (Fig. 1c, d), indicating that Cp1 might influence a pharmacodynamic mechanism that impinges on alcohol sedation. Interestingly, despite Cp1 being endogenously expressed in neurons⁴², pan-neuronal expression (via elav-Gal4) of a Cp1 RNAi transgene (v13959) did not alter ST50 values compared to Gal4 and RNAi transgene controls (Supplementary Fig. 2a). Taken together, these results suggest that Cp1 influences alcohol sedation via a role in glia. Although our studies are consistent with the hypothesis that Cp1 function in neurons might not play a major role in alcohol sedation, further studies would be required to formally assess this possibility.

The principal RNAi transgenes used in this study (v13959 and HMS00725) are predicted to target all four mRNA transcripts of Cp1 (Supplementary Fig. 3) and have no predicted off-target effects^48,49,50. We used whole-brain immunofluorescence to address whether the RNAi transgenes knockdown Cp1 expression in specific tissues. Overall Cp1 immunofluorescence was substantially reduced (v13959: 55%; HMS00725: 62%) in brains from flies with pan-glial expression of Cp1 RNAi transgenes (Fig. 1f, h) compared to brains from flies with the Cp1 RNAi transgenes alone (Fig. 1e, g). The remaining Cp1 immunofluorescence is consistent with Cp1 expression in neurons, which should not be impacted by expression of Cp1 RNAi in glia. Additionally, overall Cp1 immunofluorescence was reduced 29% in brains expressing the v13959 Cp1 RNAi transgene pan-neuronally (Supplementary Fig. 2c) compared to brains containing the v13959 Cp1 RNAi transgene alone (Supplementary Fig. 2b). The remaining Cp1 immunofluorescence is consistent with Cp1 expression in glia. These results confirm that expression of the Cp1 RNAi transgenes knocked down Cp1 as expected in both glia and neurons.

Orthologous Cp1 rescues Cp1 knockdown

When expressed in glia, both of the main Cp1 RNAi transgenes used in our studies (v13959 and HMS00725) make flies sensitive to alcohol sedation and knockdown Cp1 expression (Fig. 1). The target sequence of HMS00725 is wholly encompassed by that of v13959 (Supplementary Fig. 3), raising the possibility that the sensitivity to alcohol sedation in flies expressing Cp1 RNAi might be due to knockdown of Cp1 or another, unidentified, gene. To address this possibility, we determined whether expression of a Cp1 ortholog from Drosophila pseudoobscura in glia could rescue the alcohol sedation sensitivity in flies expressing RNAi against endogenous melanogaster Cp1 also in glia⁵¹. We choose the Drosophila pseudoobscura Cp1 ortholog (GA25021) for these studies because its primary amino acid sequence is 70–92 % similar to the four Drosophila melanogaster Cp1 isoforms and the HMS00725 siRNA target sequence is poorly conserved between Cp1 and GA25021—there are six base pair mismatches (Supplementary Fig. 4). Taken together, these findings suggested that GA25021 protein would have a similar function to melanogaster Cp1, but importantly the GA25021 mRNA would largely escape RNAi-mediated degradation by HMS00725. We therefore postulated that expression of GA25021 might rescue the alcohol sedation sensitivity observed in flies expressing RNAi against melanogaster Cp1 in glia.

We generated UAS-GA25021 transgenic flies via P-element transgenesis and then selected six lines with transgenes that didn’t impact ST50 values in the absence or presence of repo-Gal4 (see Fig. 2a, c). We then assessed whether expression of these UAS-GA25021 transgenes rescued alcohol sensitivity in constitutive glial Cp1 knockdown flies. repo-Gal4/+ flies were used as a representative control in our subsequent rescue experiments because their ST50 values were not significantly different from other control flies that had the RNAi transgene alone, the UAS-GA25021 transgene alone, or repo-Gal4 driven expression of a UAS-GA25021 transgene (Fig. 2a, c).

Consistent with the data in Fig. 1b, flies that constitutively expressed the Cp1 RNAi transgene HMS00725 in all glia (via repo-Gal4) had decreased ST50 values compared to control flies with repo-Gal4 alone (Fig. 2b, d). In contrast, flies with pan-glial expression of both the Cp1 RNAi transgene HMS00725 and a UAS-GA25021 transgene had increased ST50 values compared to flies expressing only the Cp1 RNAi transgene HMS00725 and statistically indistinguishable ST50 values compared to control flies with repo-Gal4 alone (Fig. 2b, d). In total, we tested six UAS-GA25021 transformants. The transgenes in four of the transformants rescued the glial Cp1 RNAi alcohol sedation phenotype (including those in Fig. 2), while two of the transgenes did not. The ability of Drosophila pseudoobscura Cp1 to rescue ethanol sedation sensitivity due to knockdown of melanogaster Cp1 strongly supports a role for Cp1 in glia in ethanol sedation.

We used whole-brain immunofluorescence to address whether the UAS-GA25021 transgenes expressed detectable levels of immunoreactive Cp1-like protein. Endogenous Cp1 was readily detectable in control repo-Gal4/+ brains (Fig. 2e). This signal was reduced substantially by expression of HMS00725 Cp1 RNAi in all glia (Fig. 2f; decreased 68%) and increased by expression of UAS-GA25021 transgene #1 in all glia (Fig. 2g; increased 37%). Expression of this same UAS-GA25021 transgene concurrently with HMS00725 substantially increased the Cp1 signal compared to brains that expressed only HMS00725 in all glia (Fig. 2h; increased 331%). Similarly, expression of UAS-GA25021 transgene #3 in all glia increased the Cp1 signal (Fig. 2i; increased 32% compared to repo-Gal4 alone) and expression of this same UAS-GA25021 transgene concurrently with HMS00725 substantially increased the Cp1 signal compared to brains that expressed only HMS00725 in all glia (Fig. 2e; 188%). Although we were surprised by—and do not at this time understand—the difference in Cp1 signal in flies with concurrent expression of GA25021 and HMS00725, these data indicate that the UAS-GA25021 transgenes are functional at the protein expression level. Additionally, we used real-time PCR to assess Cp1 and GA25021 expression in these studies (Supplementary Fig. 5). As expected, using primers for Cp1 we readily detected a product in cDNA samples derived from control repo-Gal4/+ and from repo-Gal4/UAS-GA25021 flies, and detection of this product was significantly reduced in flies expressing the HMS00725 Cp1 RNAi alone in glia or in combination with UAS-GA25021 (Supplementary Fig. 5, blue symbols). Also as expected, using primers for GA25021 we readily detected a product in repo-Gal4/UAS-GA25021 flies without or with concurrent expression of the Cp1 RNAi HMS00725, and this product was not detectable in repo-Gal4/+ or repo-Gal4/HMS00725 flies (Supplementary Fig. 5, red symbols). These data confirm that the Cp1 and GA25021 primers are specific for each product, that the Cp1 RNAi HMS00725 knocked down Cp1 with or without GA25021 expression, and that GA25021 is expressed with or without expression of Cp1 RNAi. The most parsimonious interpretation of the data in Fig. 2 and Supplementary Fig. 5 is that flies expressing the Cp1 RNAi HMS000725 transgene and an orthologous gene via the UAS-GA25021 transgene in glia have decreased Cp1 levels while expressing GA25021, thereby leading to the rescue of the behavioral phenotype due to Cp1 knockdown in glia.

Cp1 expression in cortex glia regulates alcohol sedation

Adult Drosophila have five CNS glial subtypes: astrocytes, ensheathing cells, cortex glia, subperineural glia, and perineural glia¹². To address the possibility that Cp1 influences alcohol sedation by functioning within one or more specific glial subtypes, we determined whether expression of Cp1 RNAi transgenes in individual glial subtypes (via a series of Gal4 drivers) altered alcohol sedation sensitivity. Flies expressing Cp1 RNAi transgenes (v13959 and HMS00725) in cortex glia (via NP2222-Gal4²⁴ or CtxGlia Split-Gal4²³) had decreased ST50 values compared to control flies with the Gal4 and RNAi transgenes alone (NP2222-Gal4: Fig. 3a, b; CtxGlia Split-Gal4: Supplementary Fig. 6a, b). Flies expressing the v110619 RNAi transgene in cortex glia (via-NP2222-Gal4) had inconsistent results (Supplementary Fig. 1b). Additionally, ST50 values were not altered by expression Cp1 RNAi (v13959) in the four other CNS glial subtypes (astrocytes, ensheathing cells, subperineural glia, and perineural glia via Alrm-Gal4¹², TIFR-Gal4²⁴, mz0709-Gal4²⁴, Gli-Gal4⁵² and Indy-Gal4¹¹) (Supplementary Table 1). The simplest interpretation of these data is that Cp1 influences alcohol sedation by functioning in cortex glia.

We used whole-brain immunofluorescence to determine if Cp1 is expressed in adult Drosophila cortex glia. Utilizing flies that constitutively express mCD8::GFP in cortex glia via NP2222-Gal4, we found that Cp1 immunofluorescence colocalized with GFP (Fig. 3c–e). When quantified using Volocity™ 3D image analysis software, >60% of the red and green pixels overlapped (average Pearson’s correlation = 0.622; n = 6). This result indicated that endogenous Cp1 is expressed in cortex glia, consistent with a role for Cp1 in acute alcohol sedation sensitivity.

Cp1 in rapid tolerance development

Flies develop rapid tolerance to alcohol, defined as increased ST50 values during a second alcohol exposure after recovering from a first alcohol exposure⁵³. To determine whether Cp1 influences this aspect of alcohol behavior through its function in CNS glia, we expressed Cp1 RNAi transgenes in all glia (via repo-Gal4) and then assessed rapid tolerance development. As anticipated, pan-glial knockdown of Cp1 via RNAi transgene v13959 significantly decreased ST50 values during the first ethanol exposure (black bars, E1) as compared to Gal4 and RNAi transgene alone controls (Fig. 4a). In contrast, ST50 values during the second alcohol exposure (gray bars, E2) were not affected by Cp1 knockdown (Fig. 4a). When quantified as the ratio between the second and first ST50 values⁵³, flies with Cp1 knocked down in all glia had an increase in the development of rapid tolerance compared to controls (Fig. 4b). As we found during the first alcohol exposure (Fig. 1c), there was no effect of knocking-down Cp1 in all glia on internal alcohol levels during the second alcohol exposure (One-way ANOVA, p = 0.85, n = 6). Knockdown of Cp1 specifically in cortex glia (via NP2222-Gal4) also reduced ST50 values during the first, but not the second, alcohol exposure (Fig. 4c), leading to an apparent increase in development of rapid tolerance compared to controls (Fig. 4d). Given that Cp1 knockdown does not significantly impact ST50 values during the second alcohol exposure (Fig. 4a, c), the most parsimonious interpretation of these data is that the increased development of rapid tolerance is likely a mathematical product of the enhanced sensitivity to alcohol during the first exposure. We therefore did not further investigate the potential role of Cp1 in rapid tolerance.

Cp1 knockdown in adult glia alters alcohol sedation

CNS glia play important roles during both development^16,54,55 and adulthood^16,19,39. To determine if Cp1 expression in glia during adulthood is important for alcohol sedation, we used the steroid-inducible GeneSwitch system⁵⁶. Flies with both the GliaGS driver and a Cp1 RNAi transgene, and control flies with either GliaGS or the RNAi transgene alone, were reared to adulthood in the absence of the steroid mifepristone (RU486) and then switched to food medium containing steroid (RU486) or vehicle for 6 days. This RU486 feeding regimen did not alter ST50 values in control flies (see GliaGS/+ control in Fig. 5a, b). In this experimental design, the Cp1 RNAi transgene should be induced in RU486-exposed adult flies harboring both a GeneSwitch Gal4 driver and an RNAi transgene⁵⁶, thereby allowing Cp1 knockdown during adulthood. Compared to vehicle control animals of the same genotype, GliaGS/v13959, GliaGS/HMS00725, and GliaGS/v110619 flies fed RU486 had decreased ST50 values (v13959 and HMS00725: Fig. 5a, b; v110619: Supplementary Fig. 1c). Exposure to RU486 in flies with either the GliaGS alone (GliaGS/+) or the Cp1 RNAi transgenes alone (v13959/+, HMS00725/+ and v110619/+) did not alter ST50 values (Fig. 5a, b and Supplementary Fig. 1c). Manipulation of Cp1 in glia during adulthood was therefore sufficient to increase alcohol sedation. Interestingly, substantial overexpression of Cp1⁴¹ (87%, quantified via immunofluorescence) in glia during adulthood did not change ST50 values (Supplementary Fig. 7). These results are consistent with a model in which endogenous, physiological levels of Cp1 in glia are required and sufficient for normal alcohol sedation in flies, suggesting that biologically relevant levels of Cp1 in glia contribute to alcohol behaviors.

To confirm that GliaGS-induced UAS transgenes are expressed in glia, we performed whole-brain immunofluorescence on flies with GliaGS driving induced expression of a UAS-LacZ transgene (i.e., GliaGS/LacZ flies fed RU486 for 6 days). Endogenous repo expression (a marker for glia, Fig. 5c) and induced LacZ expression driven by GliaGS (Fig. 5d) were detected in broad patterns throughout the fly brain that overlapped considerably (Fig. 5e). When quantified using Volocity™ 3D image analysis software, >90% of the red and green pixels overlapped (average Pearson’s correlation = 0.915; n = 7). Additionally, we found that the RU486 exposure regimen used in our behavioral studies (Fig. 5a, b) increased UAS-LacZ expression in whole fly extracts (Fig. 5f), demonstrating RU486-induced UAS-transgene expression. Taken together, these data indicate that GliaGS expresses UAS transgenes in glia in response to RU486 treatment, and therefore that Cp1 influences alcohol sedation by functioning during adulthood in CNS glia.

Discussion

Our understanding of the molecular-genetic basis for alcohol-related behavior in Drosophila and other model systems is based primarily on the results of studies that have focused on neuronal genes and mechanisms⁷. The nervous systems of flies and mammals also contain numerous classes of glia with conserved cellular-molecular activities. Given that mammalian glia respond to alcohol administration^57,58,59, that rodent astrocytes in the nucleus acumbens influence the motivation for alcohol consumption, and that surface glia influence alcohol sedation and tolerance in flies^9,10,11, it is likely that glia play direct—but underappreciated—roles in behavioral responses to alcohol.

Here, we used tissue-specific RNAi-mediated knockdown and trans-species rescue of RNAi to explore this possibility. Pan-glial Cp1 knockdown via RNAi increased alcohol sedation. Expression of an orthologous gene, Drosophila pseudoobscura GA25021, in all glia rescued the alcohol sedation phenotype due to knockdown of endogenous Cp1. Taken together, these results indicate that Cp1 expression in glia regulates alcohol sedation. Additionally, our studies found that Cp1 expression specifically in cortex glia, and probably not other CNS glia, influences alcohol sedation. The magnitude and direction of change in alcohol sedation observed when Cp1 was knocked down in all glia vs. only cortex glia were similar, suggesting that cortex glia are the principal cell type in which Cp1 functions to regulate alcohol sedation. These results reveal a previously unidentified role for Cp1 and cortex glia in Drosophila alcohol sedation. Thus, perineural glia^10,11 and cortex glia (our results) influence behavioral responses to alcohol in Drosophila.

Glia have prominent roles in nervous system development in flies^23,54. Major changes in Drosophila nervous system development—in response to altered glial cell function—could, in principle, alter alcohol sedation sensitivity. Our data indicate that manipulation of Cp1 in glia during adulthood is sufficient to alter alcohol sedation in flies. Our findings are therefore consistent with a model in which Cp1 dynamically regulates adult glial cell function, and those changes in adult glial cell function influence the response of the nervous system to alcohol.

To date, a few studies have investigated the role of Drosophila cortex glia in behavior. One study suggests that innexin2 expression in cortex glia is required for normal sleep patterns⁶⁰, and two studies have indicated that cortex glia function contributes to seizures^22,61. Additionally, cortex glia morphology influences larval locomotor behaviors²³. The results reported here add to the emerging literature on cortex glia and behavior by showing that cortex glia, via Cp1 function, influence alcohol sedation. It could be important to explore the role of cortex glia, in conjunction with Cp1 and other candidate pathways, in behavioral responses to other drugs of abuse.

Cp1 knockdown in glia, specifically cortex glia, appeared to enhance alcohol rapid tolerance development. However, glial Cp1 knockdown influenced sedation during the first exposure to alcohol only. These results suggest that Cp1 function in glia selectively influences alcohol sedation during an initial exposure to the drug and any interpretations regarding the role of Cp1 in rapid tolerance should be made with considerable caution. Importantly, though, since Cp1 knockdown in glia did not influence alcohol sedation during a second alcohol exposure or alter locomotor abilities in the absence of alcohol (Supplementary Fig. 8), it seems unlikely that the initial sedation sensitivity of flies with Cp1 knockdown in glia is related to global sluggishness, a lack of overall behavioral fitness, or other experimental artifacts. We therefore posit that glial Cp1 plays a direct role in response of the central nervous system to alcohol.

Cp1 cleaves, and thereby activates, the transcription factor cut⁴². Additionally, the protein crammer binds to and inactivates the Cp1 protein³⁶. We consequently predicted that altered expression of cut or crammer might alter sedation sensitivity. Surprisingly, constitutive expression of RNAi against cut or crammer in cortex glia or adult-specific expression of RNAi in all glia failed to substantively alter alcohol sedation (Supplementary Figs. 9 and 10). Although additional follow-up studies would be required to formally rule out a role for cut or crammer in Cp1-dependent alcohol sedation, our data suggest that Cp1 influences alcohol sedation independently of these two known genes.

Cp1 is structurally and functionally homologous to mammalian Cathepsin L⁴³. Cathepsins are powerful hydrolytic cysteine proteases and are inactively stored in the lysosomes of most tissues in mammalian cells⁶². When released from lysosomes in their active form, they play roles in many physiological processes⁶². Although Cathepsin L has not been directly implicated in alcohol-related behaviors in mammals, Cathepsin L contributes to alcohol-induced cellular and/or organ damage. For example, Cathepsin L mediates alcohol-induced pancreatic damage and alcoholic liver fibrosis^63,64. Following alcohol administration, Cathepsin L is activated in pancreatic lysosomes^63,65 and down-regulated in the cellular matrix in the liver⁶⁴, contributing to disease pathologies. However, it is unlikely that altered alcohol sedation in Cp1 knockdown flies is caused by over-all cathepsin-related glial cell damage because flies with Cp1 knockdown have normal locomotor responses in the absence of alcohol (Supplementary Fig. 8) and Cp1 knockdown selectively alters alcohol sedation during a first, but not a second, exposure to the drug (Fig. 4a). Additionally, Cp1 overexpression in all glia during adulthood does not alter alcohol sedation (Supplementary Fig. 7). Although our results do not rule out the possibility that Cp1 is involved in glial cell damage, they do suggest that alcohol sedation sensitivity in Cp1 knockdown animals is unrelated to cellular damage that potentially may be occurring.

Cathepsin L also functions in secretory vesicles as a proneuropeptide processing⁶⁶. Cathepsin L knockdown resulted in an 80–90% reduction of Neuropeptide Y (NPY) production in mammals⁶⁶. Interestingly, NPY is synthesized in glia during development and adulthood in mammals. During adulthood, glial NPY is postulated to provide trophic support to neurons⁶⁷. Mammalian NPY is homologous to Drosophila Neuropeptide F (NPF), which influences alcohol sedation in Drosophila^68,69. While a role for Cp1 in NPF maturation in flies is possible, it seems unlikely that glial Cp1 influences alcohol sedation via processing of NPF. When NPF synthesis was ablated in all NPF-producing cells, alcohol sedation was blunted⁶⁹, whereas knockdown of Cp1, which would also be predicted to decrease NPF production, increased alcohol sedation in our studies. These contradictory results make it very unlikely that Cp1 and NPF are working in conjunction to mediate alcohol sedation in Drosophila. Thus, additional studies, potentially involving approaches grounded in proteomics, are required to begin elucidating the molecular mechanisms involved in Cp1-dependent modulation of alcohol sedation in flies.

In summary, our results suggest a previously unidentified and potentially direct role for Drosophila glia in alcohol-related behaviors and that Cp1 represents a functional entry point for further understanding of cortex glial mechanisms that underlie alcohol sedation. Given that Drosophila Cp1 is orthologous to mammalian Cathepsin L, and that fly cortex glia are functionally similar to mammalian protoplasmic astrocytes, our findings have the potential to be translatable to mammalian systems. Our findings also raise the possibility that glial cysteine proteinases might mediate behavioral responses to other drugs of abuse in both flies and mammals.

Methods

Fly husbandry

All flies were reared under standard conditions⁵³. Flies were grown on food medium containing 10% sucrose, 3.3% cornmeal, 2% yeast, 1% agar, 0.2% Tegosept, and antibiotics (0.1 g/L ampicillin, 0.02 g/L tetracycline, 0.125 g/L chloramphenicol) with active dry yeast on top in 6-ounce polypropylene Drosophila bottles (Fisher Scientific, Hampton, NH). Flies were housed in an environmental chamber kept at 25 °C and 60% relative humidity with a 12-h light/dark cycle. All comparisons between groups were based on studies with flies that were grown, handled, and tested side by side.

Fly stocks

UAS-RNAi transgenic strains to manipulate Cp1 expression were obtained from commercial/public resources: v13959 and v110619, Vienna Drosophila Resource Center (VDRC), Vienna, Austria; HMS00725 (stock number 32932) and EY05806 (stock number 15957), Bloomington Drosophila Stock Center (BDSC), Bloomington, IN. HMS00725, marked with y+, was backcrossed to a w¹y¹ strain (stock number 1495, BDSC) for seven generations to normalize the genetic background. UAS-RNAi strains targeting crammer (v22751 and v22752) and cut (v4138 and v5687) were obtained from the VDRC. A w¹¹¹⁸ reference stock from the VDRC (stock number 60000) was used to control the genetic background of all flies obtained from this stock center. The UAS-LacZ transgenic strain used to validate GliaGS-induced expression was obtained from BDSC (stock number 6452). Gal4 drivers were obtained from the indicated sources: repo-Gal4 (BDSC, stock number 7415), elav-Gal4 (BDSC, stock number 8760), Alrm3-Gal4 and mz0709-Gal4 (Marc Freeman, Oregon Health Sciences University, Portland, OR, USA), NP2222-Gal4 and TIFR-Gal4 (Mary Logan, Oregon Health Sciences University, Portland, OR, USA), CtxGlia Split-Gal4 (Jaeda Coutinho-Budd, University of Vermont, Burlington, VT, USA), Indy-Gal4 (Fred Wolf, University of California–Merced, Merced, CA, USA) and Gli-Gal4 (Doris Kretzschmar, Oregon Health Sciences University, Portland, OR, USA). All Gal4 stocks (marked with mini-w) were backcrossed to our standard reference strain, w[A] (w¹¹¹⁸ in an isogenic background; BDSC, stock number 5905) for seven generations to normalize the genetic background. The steroid-inducible Gal4 driver, GliaGS, was obtained from the BDSC (stock number 59929, GeneSwitch ID 7293–1). The NP2222-Gal4 strain that constitutively expressed mCD8::GFP was provided by Mary Logan (Oregon Health Sciences University, Portland, OR, USA).

Ethanol sedation sensitivity and rapid tolerance

One day before behavioral studies, adult flies (1–4-days-old) were placed under light CO₂ anesthesia and sorted for sex. Eleven adult female flies were placed into fresh non-yeasted food vials (standard food medium without active dry yeast on top). Flies recovered in food vials stored upside down (food side up) overnight at 25 °C and 60% relative humidity. Each vial of flies corresponded to n = 1; up to 24 vials were tested in each single ethanol sedation experiment.

Ethanol sedation studies were performed at 23–25 °C and 55–65% relative humidity under standard office lighting. Flies, after a 1–2 h acclimation period in the testing room, were transferred to empty polystyrene food vials (VWR, Radnor, PA) and trapped in the vials with a cellulose acetate Flug (FlyStuff, San Diego, CA) inserted ~2 cm into each vial. The number of inactive flies was recorded for each vial (typically 0–1 flies/vial). One milliliter of 85% ethanol (made fresh weekly) was added to each Flug, and the vials were immediately sealed with a silicone stopper. Once every 6 min, each vial was tapped gently on a table three times and the number of sedated flies (i.e., still on the bottom of the vial) was recorded 30 s later. The ethanol sedation experiments were terminated when all flies were sedated, typically after 60–90 min. The percentage of active flies was calculated for each vial at each time point, and the time required for 50% of the flies in each vial to become sedated (sedation time 50, ST50) was interpolated from sigmoidal curve fits using Excel (Microsoft, Redwood, WA)^53,70.

Rapid tolerance to ethanol was assessed as the change in sensitivity to ethanol sedation due to a prior exposure to the drug. Flies were tested for ethanol sedation during a first ethanol exposure as described above (E1), returned to food vials to recover for 4 h, and then tested for ethanol sedation during a second ethanol exposure (E2)⁵³. The development of rapid tolerance was quantitated as the ratio between the ST50 during E2 and the ST50 during E1.

Internal ethanol

Flies were exposed to vapor from 85% ethanol as described for measuring ethanol sedation⁷¹. After exposure to ethanol vapor for a duration equivalent to the ST50, flies were transferred to 1.5 mL snap-cap tubes and frozen at −80 °C. Frozen flies were homogenized in 200 µL ice-cold ddH₂0 and then centrifuged at 14,000 rpm at 4 °C for 20 min. The internal ethanol concentration of the supernatant was determined using Alcohol Reagent Set (Pointe Scientific Inc., Canton, MI) according to the manufacturer’s instructions.

Trans-species rescue of the Cp1 RNAi in glia

FlyBase and NCBI were used to determine that D.melanogaster Cp1 and D. pseudoobscura GA25021 were orthologous. Fly stocks that express D. pseudoobscura GA25021 under UAS control were created via standard P-element-mediated transgenesis using pUAST⁵¹. The D. pseudoobscura GA25021 cDNA was cloned into the pUAST vector by GenScript (Piscataway, NJ, USA) and injected in w[A], our standard lab stock, by Rainbow Transgenic Flies (Camarillo, CA, USA). We mapped the independent UAS-GA25021 insertions to autosomes. Flies constitutively expressing the HMS00725 Cp1 RNAi transgene in all glia via repo-Gal4 were generated through standard crosses.

Quantitative real-time PCR

mRNA expression was assessed via quantitative real-time PCR (qRT-PCR)⁷². Approximately 400 fly heads per n were isolated. Total RNA was isolated using TRIZOL and was reverse transcribed via oligo (dT) primers and Superscript II reverse transcriptase (Invitrogen; Carlsbad, CA, USA). qRT-PCR was performed using an Applied Biosystems Fast 7500 system with SYBR Green PCR master mix (Quanta Biosciences; Beverly, MA, UAS) and run in triplicate. Each qRT-PCR experiment was performed with independent RNA isolations and cDNA syntheses, and normalized to actin5C. Primers used (forward/reverse) were as follows: Cp1, 5′- CTCATGTGACGCTGCCCAAATC-3′/5′- CCAGCACAGGCGCCCTC-3′; GA25021, 5′- GACAGCATTGATTCTTCCCCTCC-3′/5′- GTGTGCCATTCCTCCTGGATG-3′; actin5C, 5′- AGCGCGGTTACTCTTTCACCAC-3′/5′- GTGGCCATCTCCTGCTCAAAGT-3′ (Fisher Scientific, Hampton, NH, USA). Primers for Cp1 readily detected endogenous Cp1 from repo-Gal4/+ flies, but this level of expression was not significantly altered in repo-Gal4/UAS-GA25021 flies (see Supplementary Fig. 5). Additionally, primers for GA25021 detected a product in repo-Gal4/UAS-GA25021 flies, but this signal was not altered by expression of Cp1 RNAi HMS00725 (see Supplementary Fig. 5). These findings confirm that the Cp1 and GA25021 primers were specific for their intended products.

Locomotor behavior

Flies were collected as described above for ethanol sedation. On the test day, flies were transferred to empty polystyrene food vials. The positive control group vials (Gal4/+) were vortexed for 4 min prior to the experiment. Thereafter vials were handled as described for ethanol sedation studies, except for the following changes: no ethanol was placed on the flug and no plug was used to seal the vial. The percentage of active flies was calculated for each vial at each time point.

GeneSwitch induction

One-hundred microliters of 1 mM Mifepristone (RU486; Sigma Aldrich, St. Louis, MO) or vehicle (100% ethanol) was added to the surface of solidified food in vials and allowed to dry overnight. Flies were provided food medium topped with RU486 (induced) or vehicle (control) for 6 days total. Flies were transferred to fresh drug- or vehicle-treated food vials after 3 days.

β-Galactosidase activity

β-Galactosidase activity was measured in whole body extracts of flies⁷³. Three adult (4-day-old) female flies were homogenized in 250 µL buffer (1x PBS with 1x protease inhibitor cocktail (Sigma Aldrich, St. Louis, MO)). An additional 500 µL of extraction buffer was added, the extracts were vortexed and then centrifuged at 14,000 rpm for 5 min at room temperature. One-hundred microliters of the resulting supernatant was added to 900 µL of 1 mM chlorophenol red-β-d-galactopyranoside (Sigma Aldrich, St. Louis, MO). β-galactosidase activity was observed as the change in absorbance at 562 nm over 6 min in a Ultraspec 2000 spectrophotometer (Pharmacia Biotech, Piscataway, NJ).

Whole-brain imaging and immunodetection

Whole brains from adult (4-day-old) female flies were dissected in 0.3% Phosphate buffer Triton X-100 (PBT) under a dissecting microscope. Dissected brains were fixed in 0.5 mL snap-cap tubes containing 4% paraformaldehyde on ice and then for 20 min at room temperature on a tube rotator. Brains were then washed with 0.3% PBT and blocked with 5% normalized goat serum (NGS). Primary antibodies diluted in 5% NGS were added and brains were placed on a tube rotator at 4 °C for 36–48 h. Brains were washed with 0.3% PBT and exposed to the secondary antibodies diluted in 5% NGS at 4 °C for 36–48 h. Brains were then washed with 0.3% PBT and mounted onto glass slides in SlowFade mounting medium (Invitrogen, Carlsbad, CA)⁷⁴.

The following primary antibodies at the indicated concentrations from the listed sources were used: polyclonal guinea pig anti-cp1 (1:250; donated from Patrick Dolph, Dartmouth College, NH); monoclonal mouse anti-repo (1:100, Developmental Studies Hybridoma Bank, Iowa City, IA); polyclonal rabbit anti-LacZ (1:25, Fisher Scientific). The following secondary antibodies were used: goat anti-guinea pig Alexa 568, rabbit anti-mouse Alexa 488 and chicken anti-rabbit Alexa 647 (all at 1:1000; ThermoFisher, Waltham, MA).

All images were collected using a Zeiss LSM 510 multi-photon microscope (Carl Zeiss Microscopy, LLC, Thornwood, NY) or a Zeiss LSM 700 confocal microscope (Carl Zeiss Microscopy, LLC, Thornwood, NY) housed in the VCU Department of Anatomy and Neurobiology Microscope Facility. Confocal images using a pin hole of 1 Airy disc unit and Nyquist sampling were collected were collected from each adult brain. Images were taken with a 10x objective with a numerical aperture of 0.3 or a 63x oil-immersion objective with a numerical aperture of 1.4. The gain and offset values were kept constant for all images compared within an experiment.

All images taken on the Zeiss LSM 510 multi-photon microscope were processed using Zeiss LSM Image Browser Version 4,2,0,121 and Inkscape 0.92 was used to adjust image orientation. All images taken on the Zeiss LSM 700 confocal microscope were processed using Zeiss Zen 2.3. Co-localization between glia (via endogenous repo expression) and LacZ was quantified using Volocity™ 3D Image Analysis Software version 6.3. All thresholds were automatically set and Pearson Correlation was reported. Mean pixel intensity of Z-stacks was quantified using ImageJ (NIH, Bethesda, MA, USA).

Statistics and reproducibility

All statistical analyses (one-way ANOVA, two-way ANOVA, and Bonferroni’s multiple comparison tests) were performed with Prism 6.04 (GraphPad Software, San Diego, CA, USA). Numerical data are mean ± S.E.M.

The researcher was blinded to all groups in experiments whenever possible. All behavioral experiments were performed twice to ensure all data presented in the manuscript is reproducible. All molecular experiments utilized multiple individual samples to account for natural variations and ensure reproducibility.

Reporting summary

Further information on research design is available in the Nature Research Reporting Summary linked to this article.

Data availability

The authors declare that all data supporting the findings of this study are available within the article and its supplementary information files. Requests of any additional information or data that support the findings of this study are available from the corresponding author upon reasonable request.

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Acknowledgements

We thank Drs. Marc Freeman (OHSU), Doris Kretzschmar (OHSU), Mary Logan (OHSU), Jaeda Coutinho-Budd (UVM), and Fred Wolf (UC Merced) for generously donating many GAL4 driver stocks used in these studies. We also thank Dr. Patrick Dolph (Dartmouth) for donating the Cp1 antibody, Dr. Andrew Davies (VCU) for helpful comments on the manuscript, Brandon Shell for technical assistance, Rebecca Schmitt for thorough reading of the manuscript and backcrossing the HMS00725 stock, Dr. Poonam Bhandari for backcrossing the repo-Gal4 and elav-Gal4 stocks, and Dr. Jeff Dupree (VCU) for helpful discussions about microscopy methodology and co-localization quantification. We also thank the members of the VCU Alcohol Research Center for helpful discussions. Stocks obtained from the Bloomington Drosophila Stock Center (NIH P40OD018537) and Vienna Drosophila Research Center were used in this study. Microscopy was performed at the VCU Microscopy Facility, supported, in part, by funding from NIH-NCI Cancer Center Support Grant P30 CA016059. This work was supported by grants from the National Institute on Alcohol Abuse and Alcoholism (R01 AA020634, P50 AA022357, and F31 AA026500) and from the VCU Presidential Quest Fund to M.G.

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Neuroscience Graduate Program, Virginia Commonwealth University, Richmond, VA, 23298, USA
Kristen M. Lee & Mike Grotewiel
Department of Pharmacology and Toxicology, Virginia Commonwealth University, Richmond, VA, 23298, USA
Laura D. Mathies
Virginia Commonwealth University Alcohol Research Center, Virginia Commonwealth University, Richmond, VA, 23298, USA
Laura D. Mathies & Mike Grotewiel
Department of Human and Molecular Genetics, Virginia Commonwealth University, Richmond, VA, 23298, USA
Mike Grotewiel

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K.L. conceived the work together with M.G. and L.M. K.L. designed and performed all experiments. K.L. interpreted data with M.G. K.L. wrote the manuscript with input from M.G.

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Correspondence to Mike Grotewiel.

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Lee, K.M., Mathies, L.D. & Grotewiel, M. Alcohol sedation in adult Drosophila is regulated by Cysteine proteinase-1 in cortex glia. Commun Biol 2, 252 (2019). https://doi.org/10.1038/s42003-019-0492-5

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Received: 19 February 2018
Accepted: 30 May 2019
Published: 03 July 2019
DOI: https://doi.org/10.1038/s42003-019-0492-5

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