Abstract
The Pannexin 1 (Panx1) ion and metabolite channel is expressed in a wide variety of cells where it regulates a number of cell behaviours including proliferation and differentiation. Panx1 is expressed on the cell surface as well as intracellular membranes. Previous work suggests that a region within the proximal Panx1 C-terminus (Panx1CT) regulates cell surface localization. Here we report the discovery of a putative leucine-rich repeat (LRR) motif in the proximal Panx1CT necessary for Panx1 cell surface expression in HEK293T cells. Deletion of the putative LRR motif results in significant loss of Panx1 cell surface distribution. Outcomes of complementary cell surface oligomerization and glycosylation state analyses were consistent with reduced cell surface expression of Panx1 LRR deletion mutants. Of note, the oligomerization analysis revealed the presence of putative dimers and trimers of Panx1 at the cell surface. Expression of Panx1 increased HEK293T cell growth and reduced doubling time, while expression of a Panx1 LRR deletion mutant (highly conserved segment) did not reproduce this effect. In summary, here we discovered the presence of a putative LRR motif in the Panx1CT that impacts on Panx1 cell surface localization. Overall these findings provide new insights into the molecular mechanisms underlying C-terminal regulation of Panx1 trafficking and raise potential new lines of investigation with respect to Panx1 oligomerization and glycosylation.
Similar content being viewed by others
Introduction
Pannexin 1 (Panx1) is a four-transmembrane domain protein that oligomerizes in hexamers to form ion and metabolite channels, and is detected at the cell surface and intracellular membranes (reviewed in Boyce et al.1). The factors regulating Panx1 trafficking and cell surface localization have been the focus of intense study, identifying glycosylation state2,3,4 and the intracellular C-terminus5,6,7 as important contributors. The cumulative data of several studies suggests that the Panx1 C-terminus (Panx1CT) is required, but not sufficient for cell surface localization. Panx1CT deletion in BICR-M1Rk cells resulted in a loss of Panx1 cell surface localization6. Furthermore, substitution of the Panx2 C-terminus with that of Panx1 was not sufficient to direct the usually intracellular Panx2 to the cell surface in Neuro-2a (N2a) cells7. In addition, distal Panx1CT deletion mutants retain cell surface expression and/or function that is dependent on surface localization8,9. Together these previous studies suggest a region in the proximal C-terminus is important for trafficking of Panx1 to the cell surface.
In the present study, we seek to expand our understanding of Panx1CT regulation of cell surface expression. We created EGFP-tagged Panx1CT deletion mutants and examined their surface expression along with two other key properties that provide additional insight: glycosylation and oligomerization state. Panx1 exhibits 3 main glycosylation states: Gly0 (unglycosylated), Gly1 (high-mannose species), and Gly2 (complex glycosylation)2,3,4. The complex glycosylation species of wildtype Panx1 is more frequently associated with cell surface expression2,3,4, providing an important additional molecular signature when assessing surface expression. In terms of oligomerization state, it is not yet known how Panx1 cell surface expression and hexamerization3,10,11 are linked; one possibility is that Panx1 reaches the cell surface prior to oligomerization, which could have important implications for its functional properties10. We therefore investigated the localization, glycosylation, and oligomerization profiles of EGFP-tagged Panx1CT deletion mutants in HEK293T cells, which lack appreciable endogenous Panx1 expression at the protein level (however, see Sanchez-Pupo et al.12).
Our new data suggest that the proximal Panx1CT (residues R299-D378) is necessary for Panx1 cell surface localization. Within this region we identify the consensus sequence of a novel 21 amino-acid long putative leucine-rich repeat (LRR; S328-K348) motif. In general, multiple LRR motifs combine to form LRR domains, which are recognized for their ability to mediate interaction with other proteins or lipids (reviewed in Bella et al.13), to influence protein localization to specific subcellular compartments, and facilitate cell-cell contacts, amongst other roles. For example, in LRR and PDZ (LAP; PDZ, PSD-95/Discs-large-ZO1) proteins, the LRR domain is required for targeting to the basolateral membrane of epithelial cells14. We thus investigated the role of the putative C-terminal LRR motif in Panx1 cell surface localization using several complementary techniques (cell surface biotinylation, confocal microscopy, deglycosylation, cell surface oligomerization and cell proliferation analyses). Overall, this study provides important new insights into the role of the Panx1CT in cell surface localization, identifying a novel, putative LRR motif within the proximal C-terminus that is required for cell surface localization.
Results
A region in the proximal Panx1CT is required for cell surface localization
We first generated constructs with large deletions of the C-terminus (Fig. 1a): the entire C-terminus (Panx1∆299-EGFP) and the region distal to a C-terminal caspase cleavage site (Panx1∆379-EGFP; D378 is the terminal Panx1 amino acid in this deletion mutant). We used cell surface biotinylation and confocal imaging to determine the localization of Panx1-EGFP and these Panx1CT deletion mutants. There was no significant difference between surface levels of Panx1-EGFP and Panx1∆379-EGFP, consistent with previous reports8 and Panx1∆379-EGFP-expressing cells appeared to be perfectly healthy. However, we detected substantially less Panx1∆299-EGFP at the cell surface (Fig. 1b). Similarly, confocal imaging revealed co-distribution of Panx1-EGFP and Panx1∆379-EGFP with the surface marker wheat-germ agglutinin (WGA) while Panx1∆299-EGFP did not co-distribute with WGA as revealed by Pearson’s correlation coefficient analysis, a measure of the strength of the association between the fluorescence intensities of EGFP and WGA (Fig. 1c). We also investigated the glycosylation state of these C-terminus deletion mutations using deglycosylation enzymes that target immature (high-mannose) and mature (complex) glycosylation states. The glycosylation states of Panx1-EGFP and Panx1∆379-EGFP were similar to one another (Fig. 1d). The Gly1 and Gly2 bands in Panx1-EGFP and Panx1∆379-EGFP samples were sensitive to PNGase F treatment (Fig. 1di), confirming complex N-linked glycosylation, which has been previously associated with cell surface expression2,3,4. However, only Gly0 and Gly1 forms were detected for Panx1∆299-EGFP (Fig. 1d), which exhibited reduced expression levels (Supplementary Fig. S1), as previously reported6. Together these data confirm that the proximal C-terminus is required for cell surface localization of Panx1, as suggested by previous reports6,8,9.
The proximal Panx1CT is necessary for cell surface localization of Panx1-EGFP. (a) Schematic of full length Panx1-EGFP and the Panx1∆299-EGFP and Panx1∆379-EGFP deletion mutants. (b) Cell surface biotinylation assays reveal that the proximal Panx1CT is required for cell surface localization in HEK293T cells. (i) Representative Western blot of pulldown (cell surface protein) and input. Anti-EMMPRIN was used as a positive control for biotin pulldown and as a loading control, and anti-GAPDH was used as a negative control against biotin internalization. (ii) Panx1∆299-EGFP exhibited reduced cell surface levels compared to Panx1-EGFP, while surface levels of Panx1∆379-EGFP were similar to those of Panx1-EGFP. Data are presented as mean ± SEM. One-way ANOVA with Dunnett’s multiple comparisons test, N = 3, α = 0.05, F(2,6) = 62.39, **P = 0.0002, ns, non-significant. (c) Representative confocal micrographs of HEK293T cells overexpressing Panx1-EGFP, Panx1∆379-EGFP, or Panx1∆299-EGFP (green). Hoechst was used as a nuclear counterstain (blue) and wheat-germ agglutinin (WGA) was used as a plasma membrane marker (magenta). Overlapping EGFP and WGA signals (white) and scatterplots with EGFP and WGA fluorescence signals show the co-distriution of these proteins along the cell membrane. Panx1-EGFP and Panx1∆379-EGFP co-distributed with WGA, while Panx1∆299-EGFP did not. One-way ANOVA with Dunnett’s multiple comparison test, N = 3, α = 0.05; Pearson’s: F(2,6) = 62.29, ***P = 0.0002, ns, non-significant; Colocalization rate: F(2,6) = 26.81, **P = 0.0014, ns, non-significant. Scale bars, 10 μm. Data are presented as mean ± SEM. (d) Deglycosylation assays using (i) PNGase F or (ii) EndoHf reveal Panx1-EGFP and Panx1∆379-EGFP exhibited Gly0, Gly1, and Gly2 glycosylation species, while Panx1∆299-EGFP exhibited only Gly0 and Gly1 forms. Anti-pan-cadherin and anti-β-actin were used as a positive and negative controls for deglycosylation, respectively. This figure was modified from Epp 201946. For uncropped images of all Western blots in this figure, please see Supplementary Fig. S3.
To compare the cell surface oligomerization properties of Panx1∆379-EGFP and Panx1-EGFP, we used a cell-impermeable crosslinker, BS3 (Fig. 2). A similar deletion mutant, Panx1∆3788,9 formed functional cell surface channels implying it is able to oligomerize ‘appropriately’. Panx1-EGFP formed hexamers as previously reported3,10,11, as well as intermediate oligomers (corresponding to dimers or trimers). The Panx1∆379-EGFP mutant formed oligomers that corresponded to those of Panx1-EGFP (relative to molecular weight of the monomer; Fig. 2aii,b). That is, there were no significant differences in relative abundance of hexamers, or intermediate oligomers. It is important to note that under these conditions we were not able to discern whether monomeric bands originated from intracellular or cell surface pools. We did not detect high molecular weight species corresponding to intermediate oligomers or hexamers with Panx1∆299-EGFP, which was expected given its restricted cell surface localization required for BS3 exposure (Fig. 2aiii,b). We did not detect any higher molecular weight bands with longer exposures (data not shown). These results confirm that cell surface proteins formed oligomers in similar abundance, i.e. oligomerization profile was conserved with the deletion of the distal C-terminus. Overall, these results suggest that a region within the proximal Panx1CT is required for its trafficking to the cell surface and that the distal Panx1CT is dispensable for oligomerization of Panx1. We thus asked whether there are specific amino acids in the proximal Panx1CT that regulate Panx1 trafficking.
Surface oligomerization is preserved with deletion of the distal Panx1CT. (a) Crosslinking assays reveal oligomerization profiles of C-terminal deletion mutants. Representative Western blots observing HEK293T cell lysates post-transfection with (i) Panx1-EGFP, (ii) Panx1∆379-EGFP, or (iii) Panx1∆299-EGFP, and after crosslinking with the cell-impermeable crosslinker, BS3, to observe surface-localized oligomers (6× or ~2–3×). The plot of each quantification is included to shed light on the analytical process. An antibody for Crmp2, an intracellular protein known to form tetramers, was used as a negative control to ensure BS3 had not entered the cell. Crmp2 oligomerization did not increase in the presence of BS3, as expected. (b) Each oligomeric band was quantified and expressed as a percentage of the entire GFP signal in each lane. Non-specific bands were excluded from the analysis. Mutants lacking the distal Panx1CT (Panx1∆379-EGFP) had the same oligomerization profiles as full length Panx1-EGFP. Data are presented as mean ± SEM. One-way ANOVA with Dunnett’s multiple comparisons test, N = 3, α = 0.05; F(2,6) = 17.59, **P = 0.0023 (6×); F(2,6) = 15.73, *P = 0.0127 (3×); F(2,6) = 13.84, *P = 0.0169 (1×); ns, non-significant. All samples were derived from the same experiment and processed in parallel. This figure was modified from Epp 201946. For uncropped images of all Western blots in this figure, please see Supplementary Fig. S4
A putative leucine-rich repeat motif in the proximal Panx1CT is required for cell surface localization
The ScanProsite tool15 was used to detect previously unidentified motifs within the mouse Panx1 amino acid sequence (NCBI accession number: NP_062355.2) that could contribute to Panx1 localization. The scan (using high-sensitivity settings) uncovered a previously unreported putative leucine-rich repeat (LRR) motif at residues S328-K348 (Fig. 3). Further examination revealed the putative LRR motif perfectly fit the criteria: single LRRs typically consist of a highly conserved segment (HCS) of 11–12 residues consisting of LxxLxLxxNxL or LxxLxLxxCxxL; where L is Leu, Ile, Val, or Phe (but can be replaced by any hydrophobic amino acid); N is Asn, Thr, Ser, or Cys; and C is Cys, Ser, or Asn; and where the first and last L can be replaced by relatively hydrophilic residues, followed by a variable segment (VS)13,16,17,18,19,20. As multiple (2 or more) LRR motifs are usually found in relatively close proximity in a protein sequence to form solenoidal LRR domains13,20, we further inspected the amino acid sequence for adjacent LRR motifs within the Panx1CT. We discovered an additional 4 sequences consistent with the HCS consensus criteria and numbered each segment HCS1-HCS5 (Fig. 3). These sequences are conserved between mouse, human, and rat Panx1CT. As these additional HCS sequences were not identified by ScanProsite, we were not able to identify prospective VS included within each corresponding LRR motif.
The Panx1CT amino acid sequence contains 5 LRR HCS consensus sequences. Sequence alignment of Panx1CT from human, mouse, and rat. Each of the identified HCS regions are indicated in red, and labeled with the corresponding consensus sequence. The full LRR identified by ScanProsite was separated into an HCS and VS (the VS is indicated in purple). The alignment was generated using CLUSTAL O (1.2.3) with NCBI accession numbers: NP_056183.2 (human), NP_062355.2 (mouse), and NP_955239.1 (rat). This figure was modified from Epp 201946.
In light of the discovery of the putative LRR motif and the additional LRR HCS, together with previously reported shared ancestry and sequence conservation between Panx1 and LRRC8A, an LRR-containing protein21, we investigated the hypothesis that these C-terminal putative LRR motifs play a role in Panx1 cell surface localization. We started with the putative LRR motif at S328-K348 identified by ScanProsite (containing an HCS and an identified VS). We generated a full S328-K348 LRR motif deletion mutant (Panx1∆LRR-EGFP) as well as 2 partial LRR deletion mutants targeting separately the HCS at S328-L341 (Panx1∆HCS-EGFP) and the VS at K342-K348 (Panx1∆VS-EGFP; Fig. 4a). We then examined the localization and glycosylation states of these mutants. Panx1∆LRR-EGFP and Panx1∆HCS-EGFP exhibited drastically decreased surface localization, as determined by cell surface biotinylation (Fig. 4b) and confocal imaging (Fig. 4c). Additionally, only Gly0 and Gly1 forms were detected for Panx1∆LRR-EGFP and Panx1∆HCS-EGFP consistent with restricted cell surface expression (Fig. 4d). Conversely, Panx1∆VS-EGFP exhibited a slight yet significant decrease in subcellular distribution and had glycosylation profiles similar to full length Panx1-EGFP. Panx1∆VS-EGFP formed oligomers consistent with those of Panx1-EGFP (Fig. 5). Unexpectedly, we detected intermediate bands for Panx1∆LRR-EGFP and Panx1∆HCS-EGFP despite their drastic reduction in cell surface localization (and therefore reduced exposure to crosslinker). These intermediates were broader than those detected in Panx1-EGFP and Panx1∆VS-EGFP samples and were also faintly detectable in samples that had not been exposed to any crosslinker (data not shown).
A novel putative LRR motif in the Panx1CT is necessary for trafficking Panx1-EGFP to the cell surface. (a) Schematic of full length Panx1-EGFP and the Panx1∆LRR-EGFP, Panx1∆HCS-EGFP, and Panx1∆VS-EGFP deletion mutants. (b) Cell surface biotinylation assays reveal that the putative LRR motif, or HCS alone, is required for cell surface localization, while the VS is also mildly important. (i) Representative Western blot of pulldown (cell surface protein) and input. Anti-EMMPRIN was used as a positive control for biotin pulldown and as a loading control, and anti-GAPDH was used as a negative control against biotin internalization. (ii) Panx1∆LRR-EGFP and Panx1∆HCS-EGFP exhibited markedly reduced cell surface levels compared to Panx1-EGFP, while Panx1∆VS-EGFP exhibited a relatively small reduction cell surface levels compared to Panx1-EGFP. Data are presented as mean ± SEM. One-way ANOVA with Dunnett’s multiple comparisons test, N = 3, α = 0.05, F(3, 11) = 152.4, ***P = 0.0007, ****P < 0.0001, ns, non-significant. (c) Confocal images of HEK293T cells overexpressing Panx1-EGFP, Panx1∆LRR-EGFP, Panx1∆HCS-EGFP, or Panx1∆VS-EGFP (green). Hoechst was used as a nuclear counterstain (blue) and wheat-germ agglutinin (WGA) was used as a plasma membrane marker (magenta). Overlapping EGFP and WGA signals (white) and scatterplots with EGFP and WGA fluorescence signals show the co-distribution of these proteins along the cell membrane. While both Panx1-EGFP and Panx1∆VS-EGFP co-distributed with WGA, Panx1∆LRR-EGFP and Panx1∆HCS-EGFP did not. N = 3, α = 0.05; Pearson’s: F(2,6) = 32.94, ***P = 0.0002, ns, non-significant; Colocalization rate: F(2,6) = 38.99, ***P = 0.0002, ****P < 0.0001, ns, non-significant. Scale bars, 10 μm. Data are presented as mean ± SEM. (d) Deglycosylation assays using (i) PNGase F or (ii) EndoHf reveal Panx1-EGFP and Panx1∆VS-EGFP exhibited Gly0, Gly1, and Gly2 glycosylation species, while Panx1∆LRR-EGFP and Panx1∆HCS-EGFP exhibited only Gly0 and Gly1 forms. Anti-pan-cadherin and anti-β-actin were used as a positive and negative controls for deglycosylation, respectively. This figure was modified from Epp 201946. For uncropped images of all Western blots in this figure, please see Supplementary Fig. S5.
Surface hexamers are not detected with complete putative LRR motif or HCS sequence deletion. (a) Crosslinking assays reveal oligomerization profiles of LRR motif deletion mutants. Representative Western blots observing HEK293T cell lysates post-transfection with (i) Panx1-EGFP, (ii) Panx1∆LRR-EGFP, (iii) Panx1∆HCS-EGFP, or (iv) Panx1∆VS-EGFP, and after crosslinking with the cell-impermeable crosslinker, BS3, to observe surface-localized oligomers (6× or ~2–3×). Higher exposures of the regions (above 116 kDa) were included to better illustrate the presence or absence of high molecular weight bands. (b) Individual oligomeric bands were quantified and expressed as a percentage of the entire GFP signal in each lane. Non-specific bands were excluded from the analysis. Data are presented as mean ± SEM. One-way ANOVA with Dunnett’s multiple comparisons test, N = 3, α = 0.05, F(3,8) = 10.59, **P = 0.0072 (6×), ns, non-significant. All samples were derived from the same experiment and processed in parallel. This figure was modified from Epp 201946. For uncropped images of all Western blots in this figure, please see Supplementary Fig. S6.
Together, these data identify a putative LRR motif in the Panx1CT and reveal its critical role in Panx1 cell surface localization.
Panx1-EGFP regulation of HEK293T cell proliferation requires the LRR motif
Our previous work suggested that Panx1 promotes N2a cell proliferation22 through as yet unknown mechanisms. Here we investigated the impact of expression of Panx1-EGFP, and in parallel expression of the Panx1∆HCS-EGFP deletion mutant exhibiting reduced cell surface localization, on the growth and proliferation of HEK293T cells (Fig. 6). Analysis of growth curves (Fig. 6ai,ii) revealed significant differences between Panx1-EGFP- and Panx1∆HCS-EGFP-expressing HEK293T cells. At 72 h, the abundance of Panx1-EGFP-expressing cells was significantly larger than that of EGFP-expressing control cells, whereas the abundance of Panx1∆HCS-EGFP-expressing cells was similar to control cell levels (Fig. 6ai,ii). By 96 h, however, both Panx1 wildtype and deletion mutant transfected cells exhibited similar cell counts, with both significantly more abundant than control cells (Fig. 6ai,ii). In terms of rate of cell division, Panx1-EGFP-expressing cells exhibited a significantly shorter doubling time than EGFP-expressing controls, while Panx1∆HCS-EGFP-expressing cells were not significantly different from controls (Fig. 6aiii). There were no differences in cell death or viability, as observed by the Trypan blue live/dead cell counts (Fig. 6aiv) and MTT assay (Fig. 6b). Overall, these data suggest the putative LRR motif is required for the effects of Panx1 on cell proliferation.
Panx1-EGFP overexpression increases HEK293T cell proliferation, but not when it lacks the HCS sequence (Panx1∆HCS-EGFP). (a) Trypan blue proliferation assays. (i) HEK293T cells overexpressing either Panx1-EGFP, Panx1∆HCS-EGFP, or EGFP control were counted at 5 time points with the first time point, T0, taking place at 24 h post-transfection (6 h after re-plating). Cell counts were used to plot growth curves. (ii) Histogram with cell counts from T0, T72, and T96. Data are presented as mean ± SEM. Two-way ANOVA with Dunnett’s multiple comparisons test, N = 4, α = 0.05, F(2,45) = 8.459, ***P = 0.0002, ****P < 0.0001, ns, non-significant. (iii) Doubling time comparisons of each condition, calculated using data from time points in the exponential growth phase. (iv) Dead cell counts at each time point, expressed as a percent of total cells. There were no significant differences in dead cell populations. Data are presented as mean ± SEM. One-way ANOVA with Dunnett’s multiple comparisons test, N = 4, α = 0.05, F(2,9) = 8.681, **P = 0.0079, ns, non-significant. (b) MTT assays performed at T72 on HEK293T cells overexpressing either EGFP, Panx1-EGFP, Panx1∆HCS-EGFP, or untransfected cells treated with a toxic dose (200 μg/mL) of cycloheximide (CHX) confirm that differences in cell numbers were not due to alterations in cell viability. Data are presented as the mean percentage of untransfected control ± SEM. One-way ANOVA with Dunnett’s multiple comparisons test, N = 6, α = 0.05, F(3,20) = 17.35, ****P < 0.0001, ns, non-significant. This figure was modified from Epp 201946.
Discussion
Discovery of a consensus sequence for a putative LRR motif within the proximal Panx1CT provides new mechanistic insight into the molecular determinants of Panx1 trafficking and localization. As we anticipated, the results of our cell surface expression and glycosylation state analyses were consistent, in that mature glycosylation was observed in Panx1 deletion mutants present primarily at the cell surface, while immature glycosylation was observed in Panx1 deletion mutants exhibiting markedly reduced cell surface expression. One possible interpretation of this finding is that deletion of the LRR or HCS (exhibiting markedly reduced cell surface expression) prevents trafficking to the trans-Golgi network (TGN), the site of complex glycosylation23, thereby preventing full glycosylation. In this scenario, glycosylation is not an active determinant of localization, but rather a passive bystander. On the other hand, it has previously been proposed that glycosylation actively directs localization2,3,4. This is, in part, based on the correlation between cell surface expression and full glycosylation amongst pannexin family members, in addition to markedly decreased cell-surface expression of glycosylation-deficient mutants. However, another possible mechanism to be investigated is that instead of driving ‘initial’ trafficking to the cell surface, complex glycosylation of Panx1 could impact on retention at the cell surface. Notably, our glycosylation state analysis also revealed that all deglycosylation enzyme-treated samples exhibited immunoreactive bands presenting ‘below’ the lowest ‘Gly0’ band of the untreated sample (actin negative control signals were unaffected). This finding suggests that the previously designated Gly0 species is glycosylated, potentially adding further complexity to our understanding of Panx1 glycosylation.
It should also be noted that we cannot rule out the possibility that the trafficking defects observed with expression of the putative LRR motif deletion mutants are secondary to impairment in subunit folding or oligomerization. Whether the impact of putative LRR motif deletion manifests through these indirect mechanisms, or rather through a more direct mechanism, such as a protein-protein interaction, will be the focus of future work. Importantly, we anticipate that global and targeted amino acid substitutions will help to reveal critical residues within this putative LRR motif and begin to shed light on the underlying mechanisms. Despite these un-answered questions, in stark contrast with the distal C-terminus this work nevertheless identifies the putative LRR motif as a key element of cell surface expression.
This new information on the role of the putative C-terminal LRR motif on Panx1 subcellular distribution has important implications for understanding the functional diversity of all 3 pannexin family members (Panx1, Panx2, and Panx3). With respect to the C-termini, the relatively weak homology between the 3 pannexins, compared with the homologies between other domains, suggests that the C-termini likely impart some unique aspects to their respective biological roles and regulation (localization, function, interactions, etc.). Panx1 and Panx3 exhibit relatively similar C-terminal (i.e. length, sequence homology) and both proteins traffic to the cell surface; whereas, Panx2 has a much larger C-terminus with low relative sequence homology, and localizes to intracellular compartments4,24. The Panx1 and Panx3 C-termini are also higher in leucine content than Panx2 (~15–17% compared to 8.8%, respectively). Examination of each sequence revealed multiple regions in the C-termini matching the LRR HCS consensus sequence (Fig. 3; Supplementary Fig. S2). It is reasonable to speculate that pannexin intermixing4,25 could involve interactions between putative larger LRR domains formed by the putative LRR motifs13,26,27,28,29.
To start to investigate the possibility of a larger LRR domain within Panx1, we examined the relationship between reported secondary structures commonly associated with LRR motifs/domains and the identified putative LRR and HCS motifs. Since an HCS usually forms a β-sheet and a VS often forms an α-helix13,17,20, we examined whether previously reported30 in silico identified putative secondary structures (partially confirmed using circular dichroism) in the Panx1CT directly aligned with HCS1-HCS5 (Fig. 3; Table 1). Notably, a previously identified β-strand30 aligned with HCS5 (Table 1), though it was not followed by an α-helix, as might be anticipated. The multiple HCS consensus sequences, the general promiscuity of LRR domain secondary structure13, and the previously identified mixture of α-helices and β-strands30 suggest the presence of a putative LRR domain within the Panx1CT.
In fact, LRRC8A, a volume-regulated anion channel containing LRR domains within its C-terminus, shares sequence homology with Panx121,28,29. While the homology analysis between LRRC8A and Panx1 focused on the N-terminus to the fourth transmembrane domain21, our findings suggest that the degree of sequence and/or motif homology between C-termini could also be of potential interest. Notably, the LRRC8A hexamer is formed by a trimer of dimers29, reminiscent of the relatively abundant cell surface expressed ‘intermediate’ Panx1 oligomer(s) (Figs 2ai, 5ai) corresponding to either a dimer or trimer, previously detected in whole cell lysates3,10. A reasonable interpretation of our novel cell surface crosslinking data, is that Panx1 forms intermediate oligomers (dimers or trimers) that are stable at the cell surface.
As the Panx1CT regulates Panx1 activity (reviewed in Boyce et al.1, Chiu et al.31, Dahl 201832), our discovery of a putative LRR motif in the Panx1CT could have functional implications. Several studies suggest that the C-terminus interacts with the Panx1 channel pore in an auto-inhibitory manner33,34. One group proposed a model in which a region in the Panx1CT, I360-G370, interacts with membranes, and that this interaction facilitates Panx1CT insertion into the Panx1 pore30. Interestingly, HCS4 almost directly overlaps with the suggested membrane-interacting region (Table 1). Further, HCS3 contains a cysteine group that was implicated in channel gating33, and HCS5 overlaps with the Panx1CT caspase-cleavage site which activates Panx134,35. The potential impact of these putative HCS/LRR motifs on channel function warrants further study.
Here we observed a positive effect of Panx1 on cell growth in HEK293T cells consistent with our previous findings in N2a cells and neural precursor cells in vitro22 and in vivo36 and work from others on melanoma tumour growth37. It is important to note that in other systems (e.g. glioma38), Panx1 was instead found to suppress tumour growth. While our current understanding of the mechanisms underlying Panx1 regulation of cell growth and proliferation are limited, two reasonable theories are that Panx1 regulates cell proliferation via modulating cytoskeletal dynamics underlying cell growth and division via interactions with cytoskeleton-regulating proteins5,39,40, and/or purinergic signaling via mediating release of ATP22,41,42,43. It is important to note that these putative mechanisms are not mutually exclusive. Furthermore, more nuanced understanding of the role(s) of Panx1 in regulating cytoskeletal dynamics and purinergic signalling in various systems/contexts could help account for the differing observations. Moreover, regulation of cytoskeletal rearrangement might only partially depend on subcellular localization (for example deletion of the putative LRR motif could disrupt interactions with specific cytoplasmic proteins while leaving other interactions unperturbed). While more work is now needed to precisely determine the mechanistic underpinnings, our results support a previously identified role for Panx1 in regulating cell proliferation and implicate the newly discovered putative LRR motif.
Overall, the discovery of the putative LRR motif within the Panx1CT broadens our understanding of the molecular determinants of Panx1 trafficking and localization. This work also raises important new questions with respect to the regulation and functional implications of Panx1 oligomerization state at the cell surface.
Methods
Plasmids
The mouse Panx1-EGFP plasmid2 used in this study was a generous gift from Drs. Dale Laird and Silvia Penuela. Deletion mutants were generated using multiple rounds of PCR/overlap PCR followed by restriction digest and ligation into a vector backbone as previously described7. Briefly, overlap-extension PCR fragments were obtained from Panx1-EGFP using primers specific to regions up- and down-stream from the Panx1 gene and primers specific to regions on either side of the deletion site. The fragments were gel purified using the QIAquick Gel Extraction Kit (28704, Qiagen Sciences) and overlap-extension PCR was performed using the obtained fragments and primers for regions peripheral to the gene. The resulting PCR product was cloned into pEGFP-N1 vector. The last amino acid in the Panx1∆299-EGFP mutant is F298. The final amino acid in the Panx1∆379-EGFP deletion mutant is D378. All constructs were confirmed by sequencing (Eurofins MWG Operon LCC).
Cell culture and transfection
Human Embryonic Kidney (HEK)-293T cells (CRL-3216™, ATCC) were cultured in Dulbecco’s modified Eagle’s medium (DMEM, 11965118) supplemented with 10% FBS (12483020), and 100 U/mL penicillin and 100 μg/mL streptomycin (15140122, all from Gibco/Life Technologies). Cells were transfected with 7.5 mM linear polyethylenamine (PEI) (23966, Polysciences) at a 10:1 PEI (μL):DNA (μg) ratio.
Antibodies
Primary antibodies: anti-β-actin (1:16,000; A5441, Sigma), anti-EMMPRIN (1:1000; AF972-SP, R&D Systems), anti-GAPDH (1:6000; NB300-221, Novus Biologicals), anti-GFP mouse monoclonal (1:4000; 66002-1-lg, Proteintech), anti-GFP rabbit polyclonal (1:32,000; A6455, Molecular Probes), and anti-pan-cadherin (1:1000; 4068, Cell Signaling Technology). Secondary antibodies: horseradish peroxidase (HRP)-conjugated AffiniPure anti-rabbit immunoglobulin G (IgG, 711-035-152), HRP-conjugated AffiniPure anti-mouse IgG (715-035-150), and HRP-conjugated AffiniPure anti-goat IgG (705-035-003). All secondary antibodies were used at 1:8000 and obtained from Jackson ImmunoResearch.
Protein extractions and Western blot
Proteins were extracted and analyzed by Western blot as previously described22,39,44. Unless otherwise specified, cells were harvested 48-h post-transfection by scraping in PBS 1% IGEPAL (10 mM PBS [150 mM NaCl, 9.1 mM Na2HPO4, 1.7 mM NaH2PO4], 1% IGEPAL CA-630) supplemented with protease inhibitor cocktail at 1 μL/106 cells (stock: 0.104 mM 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride, 0.08 mM aprotinin, 4 mM bestatin hydrochloride, 1.4 mM N-(trans-epoxysuccinyl)-L-leucine 4-guanidinobutylamide, 2 mM leupeptin hemisulfate salt, 1.5 mM pepstatin-A; P8340, Sigma), 0.2 mM PMSF, 10 μM sodium orthovanadate, and 1 mM EDTA and incubated on ice for 30 min. Homogenates were centrifuged 20 min at 12,000 rpm, and supernatants were collected. Samples were heated 5 min at 95 °C in the presence of 1X sample buffer and reducing agents (10% β-mercaptoethanol (BME) and 100 mM dithiothreitol (DTT)) prior to loading on an SDS-PAGE gel. Gels were 4–20% gradient gels (4561094, BioRad) for crosslinking assays, or 10% Laemmli gels for all other assays. Protein was transferred to a 0.2 μm pore-size polyvinyldene fluoride (PVDF; 1620177, BioRad) membrane. Membrane incubations were performed in 5% skim milk powder in PBS-T (10 mM Na2HPO4, 1.25 mM NaH2PO4, 2.7 mM KCl, 137 mM NaCl, 0.1% Tween-20). Blots were quantified by densitometry using ImageJ (http://imagej.nih.gov/ij/)45.
Deglycosylation assays
Deglycosylation assays were performed using either PNGase F (P0704S, NEB) or EndoHf (P0702S, NEB) according to the manufacturer’s protocol. Mouse anti-GFP (66002-1-lg, Proteintech) was used to detect proteins of interest.
Cell surface biotinylation
Cells were washed twice with ice cold biotinylation buffer (137 mM NaCl, 2.7 mM KCl, 1.8 mM KH2PO4, 10 mM Na2HPO4, 0.5 mM MgCl2, 1 mM CaCl2; pH 7.4) then incubated in either 0.25 mg/mL EZ-LinkTM Sulfo-NHS-SS-Biotin (21331, Thermo-Fisher Scientific) in biotinylation buffer or buffer alone (negative control) for 30 min at 4 °C with gentle rocking. The reaction was quenched by adding 1 M glycine in biotinylation buffer to an end concentration of 100 mM glycine, then washing the cells twice (quick) and once for 15 min at 4 °C with gentle rocking in 100 mM glycine in biotinylation buffer. The solution was removed and lysates were prepared as described above, using TBS 1% IGEPAL (50 mM Tris, 150 mM NaCl, 1% IGEPAL CA-630; pH 7.5) and the aforementioned protease inhibitors as lysis buffer. As a preclearing step, lysates were incubated with a 50% slurry of 50 μL of PierceTM iminobiotin agarose beads (20221, Thermo-Fisher Scientific) for 1 h at 4 °C on with rotation. To isolate cell surface biotinylated proteins, 200–500 μg of total protein was incubated with 50% slurry of 50 μL of PierceTM NeutrAvidin™ agarose beads (29200, Thermo-Fisher Scientific) for 2 h at 4 °C with rotation. The beads were washed 4 times with TBS 1% IGEPAL, 4 times with high salt TBS 1% IGEPAL (50 mM Tris, 300 mM NaCl, 1% IGEPAL CA-630; pH 7.5), and twice with 50 mM Tris pH 7.5. Surface biotinylated proteins were eluted for 5 min at 95 °C on a heat block in 1X sample buffer and reducing agents (100 mM DTT and 10% BME). Eluates and inputs were analyzed by Western blot.
Fixed cell imaging
Cells were plated onto poly-D-lysine (PDL; P6407, Sigma)-coated coverslips, 24 h post-transfection at a density of 7.5 × 104 cells/cm2 and cultured for an additional 24 h period before collection. Directly before fixation cells were incubated with an AlexaFluor-647-conjugated cell surface marker wheat germ agglutinin (WGA; 5 μg/mL; Molecular Probes) in Hank’s Balanced Salt Solution (HBSS; 14170112, Gibco) for 5 min at 37 °C. Cells were quick-washed in phosphate buffered saline (PBS; 154 mM NaCl, 6.25 mM sodium phosphate monobasic, 18.75 mM sodium phosphate dibasic, pH 7.2), fixed with 4% paraformaldehyde (PFA) in PBS for 10 min, and washed thrice in PBS with the second wash containing Hoechst 33342 (1:300; H3570, Thermo Scientific) nuclear counterstain. Coverslips were mounted in VectaShield mounting medium (H-1000, Vector Laboratories, Inc.). Images were acquired with a Leica TCS SP8 confocal microscope using a 40× (1.3 NA) oil immersion objective (1024 × 1024, 2X optical zoom, pixel area 20 nm2) as 10 µm confocal z-stacks (z-size 0.5 µm) with the center of the z-stack corresponding to the optical section containing the largest central plane of most nuclei in the field of view (FOV), obtained using a contrast-based autofocus method within the Leica Application Suite (version 3.5.5., Leica Microsystems GmbH). This central optical section was used for all co-distribution (Pearson’s Correlation Coefficient) and colocalization rate (% colocalization area/foreground area) analyses performed within the Leica Application Suite software. Representative images were adjusted uniformly using Adobe Photoshop (CC 2018, 19.1.1). Imaging and analysis were performed by a researcher blind to experimental conditions.
BS3 crosslinking assays
To observe mature protein (enriched at the cell surface), transfected cells were treated with 20 μg/mL cycloheximide (CHX; C7698, Sigma) for 8 h prior to crosslinking. Cells were washed with ice-cold crosslinking buffer (20 mM PBS [150 mM NaCl, 18.6 mM Na2HPO4, 1.4 mM NaH2PO4], 1 mM CaCl2, 0.5 mM MgCl2; pH 8), and incubated in either 3 mM bis(sulfosuccinimidyl)suberate (BS3; 21580, Thermo-Fisher Scientific) in crosslinking buffer or buffer alone for 30 min on ice. The reaction was quenched by adding 1 M Tris to an end concentration of 20 mM, then incubating in quenching solution (20 mM Tris, 20 mM PBS [150 mM NaCl, 13.7 mM Na2HPO4, 6.3 mM NaH2PO4], 1 mM CaCl2, 0.5 mM MgCl2; pH 7.2) for 15 min on ice. The solution was removed and lysates were prepared and analyzed by Western blot as described above.
Trypan blue proliferation and MTT assays
Cells were transfected as described above and, at 18 h post-transfection, replated onto 35 mm dishes at 1 × 105 cells/dish for Trypan blue proliferation assays or in 96-well plates at 1 × 104 cells/well for MTT assays. Cells were counted every 24 h for 5 days (T0-96), with T0 occurring at 24 h post-transfection. Doubling time was calculated [DT = T * ln2/ln(NT=1/NT=2)] using time points within the linear logarithmic growth curve. MTT assays were performed at T72 using the Vybrant® MTT Cell Proliferation Assay Kit (V13154, Thermo-Fisher Scientific), according the manufacturer’s protocol.
Statistical analysis
Statistical analysis was performed using Prism 7 (GraphPad Software). The tests and P values for each experiment are described in figure legends.
Data Availability
The raw data or materials generated and/or analyzed during the current study are available from the corresponding author on reasonable request.
References
Boyce, A. K. J., Epp, A. L., Nagarajan, A. & Swayne, L. A. Transcriptional and post-translational regulation of pannexins. Biochimica et Biophysica Acta - Biomembranes 1860, 72–82 (2018).
Penuela, S. et al. Pannexin 1 and pannexin 3 are glycoproteins that exhibit many distinct characteristics from the connexin family of gap junction proteins. J Cell Sci 120, 3772–3783 (2007).
Boassa, D. et al. Pannexin1 channels contain a glycosylation site that targets the hexamer to the plasma membrane. J Biol Chem 282, 31733–31743 (2007).
Penuela, S., Bhalla, R., Nag, K. & Laird, D. W. Glycosylation regulates pannexin intermixing and cellular localization. Mol Biol Cell 20, 4313–4323 (2009).
Bhalla-Gehi, R., Penuela, S., Churko, J. M., Shao, Q. & Laird, D. W. Pannexin1 and pannexin3 delivery, cell surface dynamics, and cytoskeletal interactions. J Biol Chem 285, 9147–9160 (2010).
Gehi, R., Shao, Q. & Laird, D. W. Pathways regulating the trafficking and turnover of pannexin1 protein and the role of the C-terminal domain. J. Biol. Chem. 286, 27639–53 (2011).
Wicki-Stordeur, L. E., Boyce, A. K. J. & Swayne, L. A. Analysis of a pannexin 2-pannexin 1 chimeric protein supports divergent roles for pannexin C-termini in cellular localization. Cell Commun. Adhes. 20 (2013).
Dourado, M., Wong, E. & Hackos, D. H. Pannexin-1 is blocked by its C-terminus through a delocalized non-specific interaction surface. PLoS One 9 (2014).
Wang, J. & Dahl, G. Pannexin1: a multifunction and multiconductance and/or permeability membrane channel. Am. J. Physiol. Physiol. 315, C290–C299 (2018).
Chiu, Y. H. et al. A quantized mechanism for activation of pannexin channels. Nat. Commun. 8 (2017).
Wang, J. et al. The membrane protein Pannexin1 forms two open-channel conformations depending on the mode of activation. Sci. Signal. 7, ra69–ra69 (2014).
Sanchez-Pupo, R. E., Johnston, D. & Penuela, S. N-glycosylation regulates pannexin 2 localization but is not required for interacting with pannexin 1. Int. J. Mol. Sci. 19, 1–18 (2018).
Bella, J., Hindle, K. L., McEwan, P. A. & Lovell, S. C. The leucine-rich repeat structure. Cell. Mol. Life Sci. 65, 2307–2333 (2008).
Legouis, R. et al. Basolateral targeting by leucine-rich repeat domains in epithelial cells. EMBO Rep. 4, 1096–1102 (2003).
de Castro, E. et al. ScanProsite: Detection of PROSITE signature matches and ProRule-associated functional and structural residues in proteins. Nucleic Acids Res. 34, 362–365 (2006).
Kobe, B. & Kajava, A. V. The leucine-rich repeat as a protein recognition motif. Current Opinion in Structural Biology 11, 725–732 (2001).
Kajava, A. V. Structural diversity of leucine-rich repeat proteins. J. Mol. Biol. 277, 519–527 (1998).
Matsushima, N. et al. Comparative sequence analysis of leucine-rich repeats (LRRs) within vertebrate toll-like receptors. BMC Genomics 8 (2007).
Enkhbayar, P., Kamiya, M., Osaki, M., Matsumoto, T. & Matsushima, N. Structural Principles of Leucine-Rich Repeat (LRR) Proteins. Proteins Struct. Funct. Genet. 54, 394–403 (2004).
Ng, A. C. Y. et al. Human leucine-rich repeat proteins: a genome-wide bioinformatic categorization and functional analysis in innate immunity. Proc. Natl. Acad. Sci. 108, 4631–4638 (2011).
Abascal, F. & Zardoya, R. LRRC8 proteins share a common ancestor with pannexins, and may form hexameric channels involved in cell-cell communication. BioEssays 34, 551–560 (2012).
Wicki-Stordeur, L. E., Dzugalo, A. D., Swansburg, R. M., Suits, J. M. & Swayne, L. A. Pannexin 1 regulates postnatal neural stem and progenitor cell proliferation. Neural Dev. 7, 11 (2012).
Stanley, P. Golgi glycosylation. Cold Spring Harb. Perspect. Biol. 3, 1–13 (2011).
Penuela, S., Gehi, R. & Laird, D. W. The biochemistry and function of pannexin channels. Biochim. Biophys. Acta, https://doi.org/10.1016/j.bbamem.2012.01.017 (2012).
Ambrosi, C. et al. Pannexin1 and Pannexin2 channels show quaternary similarities to connexons and different oligomerization numbers from each other. J Biol Chem 285, 24420–24431 (2010).
Scott, P. G. et al. Crystal structure of the dimeric protein core of decorin, the archetypal small leucine-rich repeat proteoglycan. Proc. Natl. Acad. Sci. 101, 15633–15638 (2004).
Jin, M. S. et al. Crystal Structure of the TLR1-TLR2 Heterodimer Induced by Binding of a Tri-Acylated Lipopeptide. Cell 130, 1071–1082 (2007).
Deneka, D., Sawicka, M., Lam, A. K. M., Paulino, C. & Dutzler, R. Structure of a volume-regulated anion channel of the LRRC8 family. Nature 558, 254–259 (2018).
Kefauver, J. M. et al. Structure of the human volume regulated anion channel. Elife 7 (2018).
Spagnol, G., Sorgen, P. L. & Spray, D. C. Structural order in Pannexin 1 cytoplasmic domains. Channels 8, 157–166 (2014).
Chiu, Y.-H., Schappe, M. S., Desai, B. N. & Bayliss, D. A. Revisiting multimodal activation and channel properties of Pannexin 1. J. Gen. Physiol. 150, 19–39 (2018).
Dahl, G. The Pannexin1 membrane channel: distinct conformations and functions. FEBS Lett. 1–9, https://doi.org/10.1002/1873-3468.13115 (2018).
Wang, J. & Dahl, G. SCAM analysis of Panx1 suggests a peculiar pore structure. J. Gen. Physiol. 136, 515–527 (2010).
Sandilos, J. K. et al. Pannexin 1, an ATP release channel, is activated by caspase cleavage of its pore-associated C terminal autoinhibitory region. J. Biol. Chem., https://doi.org/10.1074/jbc.M111.323378 (2012).
Chekeni, F. B. et al. Pannexin 1 channels mediate ‘find-me’ signal release and membrane permeability during apoptosis. Nature 467, 863–867 (2010).
Wicki-Stordeur, X. E. et al. Pannexin 1 differentially affects neural precursor cell maintenance in the ventricular zone and Peri-Infarct cortex. J. Neurosci. 36 (2016).
Penuela, S. et al. Loss of pannexin 1 attenuates melanoma progression by reversion to a melanocytic phenotype. J. Biol. Chem. 287, 29184–29193 (2012).
Lai, C. P. et al. Tumor-suppressive effects of pannexin 1 in C6 glioma cells. Cancer Res 67, 1545–1554 (2007).
Wicki-Stordeur, L. E. & Swayne, L. A. Panx1 regulates neural stem and progenitor cell behaviours associated with cytoskeletal dynamics and interacts with multiple cytoskeletal elements. Cell Commun. Signal. 11 (2013).
Xu, X. et al. Probenecid Disrupts a Novel Pannexin 1-Collapsin Response Mediator Protein 2 Interaction and Increases Microtubule Stability. Front. Cell. Neurosci. 12, 124 (2018).
Bao, L., Locovei, S. & Dahl, G. Pannexin membrane channels are mechanosensitive conduits for ATP. FEBS Lett 572, 65–68 (2004).
Dahl, G. ATP release through pannexon channels. Philos. Trans. R. Soc. B Biol. Sci., https://doi.org/10.1098/rstb.2014.0191 (2015).
Lohman, A. W. & Isakson, B. E. Differentiating connexin hemichannels and pannexin channels in cellular ATP release. In FEBS Letters, https://doi.org/10.1016/j.febslet.2014.02.004 (2014).
Swayne, L. A., Sorbara, C. D. & Bennett, S. A. Pannexin 2 is expressed by postnatal hippocampal neural progenitors and modulates neuronal commitment. J Biol Chem 285, 24977–24986 (2010).
Schneider, C. A., Rasband, W. S. & Eliceiri, K. W. NIH Image to ImageJ: 25 years of image analysis. Nat. Methods 9, 671–675 (2012).
Epp, A. Role of a novel C-terminal motif in Pannexin 1 trafficking and oligomerization. http://hdl.handle.net/1828/10748 (University of Victoria, 2019).
Boyce, A. K., Prager, R. T., Wicki-Stordeur, L. E. & Swayne, L. A. Pore positioning: current concepts in Pannexin channel trafficking. Channels (Austin) 8, 110–117 (2014).
Weilinger, N. L. et al. Metabotropic NMDA receptor signaling couples Src family kinases to pannexin-1 during excitotoxicity. Nat Neurosci 19, 432–442 (2016).
Lohman, A. W. et al. S-nitrosylation inhibits pannexin 1 channel function. J Biol Chem 287, 39602–39612 (2012).
Acknowledgements
This work received operating grant support to L.A.S. from the Natural Sciences and Engineering Research Council (NSERC) [RGPIN-2017-03889]. The Leica SP8 confocal microscope was purchased with funds from the Canada Foundation for Innovation (CFI) [grant number 29462], and the BC Knowledge Development Fund (BCKDF) [grant number 804754] and the University of Victoria awarded to L.A.S. L.A.S. also received a Scholar Award from the Michael Smith Foundation for Health Research (MSFHR) and British Columbia Schizophrenia Society Foundation [grant number 5900]. A.L.E. was supported by UVic Graduate Awards. A.K.J.B. was supported by student scholarships from NSERC [PGSD 459931-2014] and the University of Victoria (President’s Research Scholarship, and Dr. Julius F. Schleicher Memorial Scholarship). L.E.W.S. was supported by Vanier Canada Graduate Scholarships (NSERC). J.C.S.A. was supported by a University of Victoria Fellowship Graduate Award. We would also like to thank Drs. Laird and Penuela for their generous gift of the mouse Panx1-EGFP plasmid. Much of this work is modified from the first-author’s University of Victoria Master’s thesis.
Author information
Authors and Affiliations
Contributions
L.A.S. and A.L.E. directed the research. All authors contributed in some way to the experimental design. A.L.E. performed all of the biochemistry experiments; S.N.E. and J.C.S.A. played major roles in the cell growth and imaging experiments, respectively. All authors wrote and revised the manuscript.
Corresponding author
Ethics declarations
Competing Interests
The authors declare no competing interests. A disclosure has been made with the University of Victoria Research Partnerships and Knowledge Mobilization, and a provisional patent application has been filed for a peptide targeting a Panx1-Crmp2 interaction (US Application No. 62/767,806) that does not relate directly to this study.
Additional information
Publisher’s note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary information
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Epp, A.L., Ebert, S.N., Sanchez-Arias, J.C. et al. A novel motif in the proximal C-terminus of Pannexin 1 regulates cell surface localization. Sci Rep 9, 9721 (2019). https://doi.org/10.1038/s41598-019-46144-5
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/s41598-019-46144-5
This article is cited by
-
Trans-Seq maps a selective mammalian retinotectal synapse instructed by Nephronectin
Nature Neuroscience (2022)
-
Pannexin 1 channels and ATP release in epilepsy: two sides of the same coin
Purinergic Signalling (2021)
-
Pannexin 1 as a driver of inflammation and ischemia–reperfusion injury
Purinergic Signalling (2021)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
