Abstract
Iron (Fe) is an essential metal involved in a wide spectrum of physiological functions. Sub-cellular characterization of the size, composition, and distribution of ferritin(iron) can provide valuable information on iron storage and transport in health and disease. In this study we employ magnetic force microscopy (MFM), transmission electron microscopy (TEM), and electron energy loss spectroscopy (EELS) to characterize differences in ferritin(iron) distribution and composition across injured and non-injured tissues by employing a rodent model of spinal cord injury (SCI). Our biophysical and ultrastructural analyses provide novel insights into iron distribution which are not obtained by routine biochemical stains. In particular, ferritin(iron) rich lysosomes revealed increased heterogeneity in MFM signal from tissues of SCI animals. Ultrastructural analysis using TEM elucidated that both cytosolic and lysosomal ferritin(iron) density was increased in the injured (spinal cord) and non-injured (spleen) tissues of SCI as compared to naïve animals. In-situ EELs analysis revealed that ferritin(iron) was primarily in Fe3+ oxidation state in both naïve and SCI animal tissues. The insights provided by this study and the approaches utilized here can be applied broadly to other systemic problems involving iron regulation or to understand the fate of exogenously delivered iron-oxide nanoparticles.
Similar content being viewed by others
Introduction
Iron (Fe) is an essential metal involved in a wide spectrum of physiological functions, including oxygen transport, enzymatic reactions, energy production, protein synthesis and DNA repair1,2,3. Iron homeostasis is regulated by several factors including, but not limited to, iron confinement, its mineral size and composition, and its oxidation state. Ferritin is the major iron storage protein found in mammalian tissues comprising of a ~6–8 nm iron core with up to 4500 iron atoms typically in the form of ferrihydrite. The size, composition and distribution of the ferritin(iron) at the subcellular level is crucial to understand iron turnover in health and disease.
Iron assessment in pathology typically employs biochemical methods e.g. serum-ferritin levels, histochemical stains and/or immuno-labeling of tissue sections4. Oxidation state of iron in tissue sections can also be identified via Perls or Turnbull’s stains or by utilizing micro-focused X-ray beams5,6. Although these basic histology approaches are useful in rapidly evaluating iron content in situ, their limited spatial resolution cannot resolve subcellular iron distribution, which can lead to misleading results such as confounding iron-rich macrophages with biogenic magnetite7,8. In addition interpretation of in-vivo iron-assessment via approaches such as MRI1,9 would benefit by a comprehensive understanding of subcellular iron distribution.
High-resolution techniques such as transmission electron microscopy (TEM) have been useful to localize ferritin(iron) at the subcellular level in neurodegenerative10,11 and other pathologies12,13, in both rodent models12, and in human tissues10,11,13. Analytical TEM approaches10,14,15,16 have elucidated how the oxidation state of iron can differ in pathological vs. physiological tissues by analyzing the iron-rich particles isolated from various pathologies17,18. In these studies, it has been recognized that tissue digestion and extraction procedure not only destroys the cellular organization of the particles but can also influence the oxidation state of iron14. However, limited studies exist on subcellular mapping of the oxidation state of iron in situ in mammalian tissues. These include the perfusion-Perls and Turnbull’s method coupled with TEM imaging, which is primarily applicable to animal models19. Analytical TEM, while more versatile, has thus far been limited to neurodegenerative diseases20. Finally, although iron has been mapped in situ using magnetism-based microscopy21,22, the effects of particle size, crystal structure, density and oxidation state23 have not been adequately evaluated in these studies.
In this study, we examined ferritin(iron) in a well-characterized rodent model of central nervous system (CNS) injury, i.e., traumatic spinal cord injury (SCI)24, and how it differs from physiological ferritin in situ at the sub-cellular level. Biochemical histological stains were utilized to verify the presence of ferritin(iron) at the injury site (spinal cord) and in the spleen (an organ well-established for its role in iron metabolism25) from naive vs. SCI animals. A magnetism based approach, namely magnetic force microscopy (MFM), was utilized to analyze magnetic signal emanating from iron-rich lysosomes present in tissue sections in-situ. TEM was utilized to characterize the cytosolic and lysosomal ferritin(iron) density as well as lysosomal size. Electron energy loss spectroscopy (EELS), an analytical TEM technique, was used to evaluate Fe3+ percent in individual ferritin cores. Our results elucidate how ferritin(iron) distribution differs across injured and non-injured tissues at the sub-cellular level, which is not evident by routine biochemical staining. In addition, variations in the ferritin(iron) density in lysosomes could impact the magnetic signal which could be utilized in interpreting iron-status by MRI in pathological vs. physiological tissues.
Results
Biochemical Evaluation of Ferritin(Iron) in Injured and Non-Injured Tissues
Injured (SCI) and naive spinal cords were evaluated for the presence of iron using the Perls and Turnbull’s histochemical stains which indicate the presence of ferric (Fe3+) and ferrous (Fe2+) iron, respectively. We observed an increased Fe3+ iron content in injured spinal cords compared to naïve spinal cords (Fig. 1). Staining for Fe2+ in injured as well as naïve spinal cord tissues revealed no signal. Immunohistochemistry (IHC) of adjacent sections revealed the presence of both H- and L-ferritin in regions positive for Perls stain in injured spinal cord. As compared to L-ferritin, the expression of H-ferritin was more diffuse in the injured spinal cord and was also present in regions with little or no visible Fe3+ iron. Very little basal ferritin expression was detectable in spinal cord from naïve rats26.
Spleen(s) harvested from SCI and naïve rats were also evaluated for the presence of ferritin(iron) using the biochemical stains described above. Spleen tissue from both naïve and SCI rats had abundant Fe3+ iron with no obvious differences in the level of Perls stain between the two groups (Fig. 2A). Turnbull’s staining showed a lower level of Fe2+ iron as compared to Fe3+ in the spleen sections with no significant difference between SCI and naïve animals. IHC of spleen tissue showed expression of L- and H-ferritin in regions that co-localized with the Perls stain (Fig. 2B). In contrast to injured spinal cord, the spatial expression of L- and H-ferritin was similar in spleens from injured and naïve rats. Thus our biochemical analyses revealed upregulation of Fe3+ iron primarily in the affected tissue (spinal cord) at sites of injury with no detectable changes in splenic iron storage in SCI animals compared to naïve.
Magnetism Based Evaluation of Ferritin(Iron) in Injured and Non-Injured Tissues
As another measure of ferritin(iron) content, we evaluated the magnetic signal emanating from regions corresponding to Perls stained adjacent sections using magnetic force microscopy (MFM). As shown in Fig. 3A–C, long-range (z ≥ 30 nm) MFM signal (negative phase shift)27 could be detected in regions corresponding to Perls stain. The sizes of these regions exhibiting MFM signal corresponded to iron-rich lysosomes as demonstrated in our earlier study21. No MFM signal could be detected from monodisperse ferritin(iron) present in the cytoplasmic regions, consistent with our earlier study21. To evaluate if the MFM signal differed across tissues from naïve vs SCI animals, we analyzed the magnitude (ϕ) and average roughness (Ra) of the regions exhibiting negative phase shift (Fig. 3D). Both ϕ and Ra showed regional variations in each sample, indicating heterogeneity in lysosomal composition. Interestingly while ϕ did not differ significantly across samples (Fig. 3E) (for z = 30) or for z = 40 or 50 nm (data not shown), Ra values were significantly higher for tissues from injured animals compared to the naïve spleen (Fig. 3D). An increased Ra, could arise from several factors such as variations in the size, density, inter-particle interactions, oxidation state or crystal structure of the underlying magnetic nanoparticles28. We therefore performed ultrastructural TEM analysis on a subset of animals to characterize these features of ferritin(iron).
Ultrastructural Evaluation of Ferritin(iron) Distribution in Injured and Non-Injured Tissues
Iron-loaded macrophages are prevalent in the acute spinal cord injury site and are maintained chronically as determined by Perls stain and basic IHC27. Thus, ferritin(iron) in spinal cord and splenic macrophages was analyzed using TEM to determine the morphology and distribution of cytoplasmic and lysosomal ferritin(iron). Uninjured spinal cords (from naïve animals) did not contain macrophages and thus were not included in these analyses. As shown in Fig. 4A–C, ferritin(iron) cores in the macrophage cytoplasm were monodispersed with an average diameter (~5 nm) comparable across all groups (Fig. 4D). Interestingly, the density of cytoplasmic ferritin(iron) significantly increased in the macrophages from both spinal cords and spleens of SCI rats compared to splenic macrophages in naïve rats (Fig. 4E). Our observations thus reveal an increase in cytoplasmic iron storage in systemic and CNS macrophage populations after SCI.
We next evaluated the lysosomal size and ferritin(iron) density in macrophage lysosomes for their potential correlation with the observed MFM signal. Lysosomes packed with ferritin(iron) could be identified as electron dense material in cells (Fig. 5A–C). Quantitative analysis of TEM images revealed that macrophages in naïve spleen had the broadest distribution of lysosomal size and density, compared to splenic and spinal macrophages from SCI animals (Fig. 5D). While there was no significant difference in average lysosome size in splenic macrophages from both animal groups, macrophages from the injured spinal cord site had significantly smaller lysosomes compared to splenic macrophages (Fig. 5E). Consistent with our observations regarding cytoplasmic ferritin(iron) density, the average density of lysosomal iron was significantly higher in the macrophages present in the spinal cord of SCI rats compared to those in the spleen of SCI or naïve animals (Fig. 5E). Splenic macrophages in SCI rats had a higher ferritin(iron) density compared to naïve rats. Thus SCI induced a shift in peripheral splenic macrophages to accumulate iron and ferritin, in a pattern similar to macrophages at the intraspinal injury site. Collectively these data extend our prior work26,29 by showing that the SCI site becomes populated with macrophages containing an abundance of cytoplasmic ferritin plus numerous small lysosomes with dense ferritin accumulation. In addition, our TEM analysis indicates that an increased Ra in the MFM data could arise due to the increased lysosomal ferritin(iron) density in tissues from SCI rats.
Oxidation State of Ferritin (iron) in the Macrophage Cytoplasm and Lysosomes
We examined the oxidation state of macrophage ferritin(iron) using EELS analysis on ultrathin spleen and spinal cord sections. EELS analysis was carried out with an energy resolution of 0.175 eV (Fig. 6A–C). Ferritin(iron) core could be identified as nanoscale bright dots in the lysosomes and cytoplasm in dark field STEM images (Fig. 6D–F). EELS spectra was acquired and analyzed from individual ferritin(iron) cores. In concordance with the Perls and Turnbull’s data above, EELS showed that the major (>80%) iron content in ferritin particles of all samples was Fe3+ with a much smaller (<20%) percent of Fe2+. Within the cytoplasm, ferritin(iron) contained ~85% Fe3+ with no significant differences across samples. However, subtle differences in the percent of Fe3+ were detected in lysosomal ferritin (Fig. 6G). The oxidation state of iron in the lysosome of injured spinal cord closely resembled that of naïve spleen, while the percent of Fe3+ in the splenic lysosomes of injured animals showed a slight (~5%) decrease. Our results thus indicate that the oxidation state of ferritin(iron) was primarily Fe3+ in all tissues and may have little effect in modulating the Ra of the observed MFM signal.
Discussion
We elucidate here how the ferritin(iron) content in a CNS injury model differs across tissues in injured and non-injured animals which is evident primarily at the ultrastructural level. As reported earlier24, routine histochemical staining confirmed an upregulation in Fe3+ (Perls iron stain) and L- and H-ferritin in the injured spinal cord at 21 days post-injury. No staining for Fe2+ (Turnbull’s iron stain) could be detected at this stage (except by the highly sensitive EELS technique). These observations are in agreement with a rodent model of kainite-induced brain injury where an upregulation of Fe3+ was detected as early as 1 week but Fe2+ positive cells could be detected only 2 months post-injury30. Further, no differences in the splenic iron or ferritin in the injured or naïve animal could be ascertained by basic histological stains.
Our in situ ultrastructural analysis revealed a significantly increased ferritin(iron) density in the cytoplasm and lysosomes of the macrophages present in the spleen and in the spinal cord of SCI rats as compared to naïve animals. This increase in macrophage ferritin(iron) content also correlated with an increase in serum ferritin for SCI rats as compared to naïve (data not shown), consistent with earlier reports31. Lysosome size and density is also understood to directly affect serum ferritin levels31 as well as cellular functions such as lysosome transport and inflammatory state of the cell32,33. It has been suggested that macrophages sequester excess iron into their cytosol which is then transported to lysosomes for degradation and recycling34. Thus SCI induced a shift in peripheral splenic macrophages to accumulate iron and ferritin, in a pattern similar to macrophages at the intraspinal injury site. A possible signal for post-SCI iron uptake by macrophages is SCI-induced TLR4 activation, which stimulates iron sequestration systemically in response to microbial invasion35. Although pathogens are absent from the spinal cord injury site, our prior work supports that post-SCI TLR4 activation promotes macrophage uptake of iron released by intraspinal hemorrhage and cell death36. The current work suggests that SCI also induces a systemic iron sequestration phenotype since splenic macrophages from SCI rats had a greater ferritin(iron) density compared to those from naïve animals. This may partially explain the post-SCI anemia noted in SCI individuals37, which is associated with SCI-related morbidities such as increased incidence and worse wound healing of pressure sores38,39.
The size of ferritin(iron) was ~ 5 nm in tissues from both naïve and injured animals. This measure is consistent with ferritin core sizes reported in various pathologies in earlier studies10. Larger (~10–200 nm) iron particles (biogenic magnetite), which have been identified in neurodegenerative brain tissue40, were not observed in our model of SCI. Further, our EELS analysis of the oxidation state of ferritin(iron) in our SCI model revealed that it is similar to physiological ferritin, consisting primarily of Fe3+ rich ferrihydrite. In contrast to our SCI model, pathological iron in prolonged brain injury30 and chronic neurodegenerative diseases10,15 has been reported to contain abundant Fe2+ rich magnetite. It has been postulated that overload and breakdown of the ferritin cage can serve as precursor for synthesis of biogenic magnetite40. In this regard, is interesting to note that the lysosomal ferritin(iron) density was highest in spinal macrophages of SCI rats, and the size(s) of these small ferritin(iron) dense lysosomes corresponds to the size of biogenic magnetite particles. It is thus plausible that formation of small, ferritin(iron)-dense lysosomes in infiltrating cells at sites of CNS injury could serve as precursor for formation of biogenic magnetite in chronic diseases. Taken together our results support the hypothesis that ferritin(iron) dense lysosomes in infiltrating cells consist initially of physiological ferritin which may undergo transformation to magnetite in chronic diseases.
We also characterized ferritin(iron) in-situ using MFM to evaluate the magnetic character of iron deposits. We could not detect MFM signal from monodisperse ferritin in the cytoplasm of all tissues examined, consistent with our earlier study21. Putative causes for this observation could be the low magnetic moment of monodisperse ferritins41 and the difference in the environment of cytoplasmic vs. lysosomal ferritin iron(core), e.g. presence of phosphates42, which may impact their magnetic properties43,44. MFM imaging could map ferritin(iron) dense regions of sizes corresponding to lysosomal areas in tissue sections, as demonstrated in our earlier study21. Long-range (z > 50 nm) MFM signal27 could be detected in regions corresponding to Perls stain with no significant difference in the magnitude of MFM signal across samples. However, the spleen and spinal cord from SCI rats were more heterogeneous in MFM signal than naïve spleen. Our TEM analysis supports that this magnetic heterogeneity is likely due to the increased lysosomal density and resulting inter-particle coupling28 of the ferritin(iron) core in tissues from SCI rats. Very few studies exist on magnetism based subcellular mapping of iron deposits in tissues. Our results demonstrate that ferritin(iron) dense lysosomes can also yield a magnetic response besides the biogenic magnetite particles reported in Alzheimer’s disease16,22.
We elucidate here how the ferritin(iron) content in a CNS injury model differs across tissues in injured and naive animals at the subcellular level even though no differences could be ascertained by basic histological stains in certain cases. For instance, no staining for Fe2+ (Turnbull’s iron stain) could be detected in the injured spinal cord at 21 days post-injury, despite positive identification by EELS revealing the high sensitivity of the EELS technique. Similarly, density of cytoplasmic and lysosomal ferritin(iron) as well as lysosomal size significantly varied across tissues. Very few studies exist on magnetism based subcellular mapping of iron deposits in tissues. These include biogenic magnetite particles reported in neurodegenerative diseases42 and other organisms45 or cells labeled with exogenously added magnetic nanoparticles46. Our results demonstrate that ferritin(iron) dense lysosomes naturally present in tissues also yield a MFM signal, which could be utilized for a more accurate assessment of iron status via approaches such as MRI47.
In summary, our results demonstrate the rich physiologically relevant data on iron status, obtainable with nanoscale methods that go beyond basic immunohistochemistry. These data provide greater insight into how iron is handled by different tissues following injury, which could in turn lead to better treatment strategies to combat systemic anemia or iron-induced cell death. The spinal contusion model used is clinically relevant as it mimics the most common injury suffered by humans. The approaches utilized here can be applied broadly to other systemic problems involving iron regulation or to understand the fate of exogenously delivered iron-oxide nanoparticles48,49 used in diagnostic or therapeutic applications.
Materials and Methods
Animal Procedure
All surgeries and postoperative care procedures were performed after approval by the Ohio State University Institutional Animal Care and Use Committee (IACUC). In addition, all methods were performed in accordance with the IACUC guidelines and regulations. Anesthetization of adult female Sprague Dawley rats was administrated via an 80 mg/kg ketamine and 10 mg/kg xylazine mixture. A laminectomy was performed at the thoracic T8 vertebral level to expose the underlying spinal cord, at which point a spinal contusion of moderate force (200 K dynes) was induced using the Infinite Horizons device (Precision Instruments). Following the injury, surrounding tissue was sutured and the skin closed with wound clips. Rats were administered 5 ml saline and placed in warm recovery cages. Antibiotics were administered for 5 days along with saline to support hydration. Bladder function was compromised; therefore, manual bladder expression was conducted twice each day until normal function returned. A group of rats not receiving SCI served as naïve controls. At 21 days post-injury, rats were deeply anesthetized (1.5× surgery dose) and then perfused transcardially with distilled water followed by fixation in 250 ml of 4% paraformaldehyde (PF) in PBS. For TEM studies the rats were perfused with a mixture of 4% PF and 2% glutaraldehyde in 0.1 M cacodylate buffer, pH 7.4. The entire spinal cord and spleen were extracted from these animals and segments of tissue from these organs were used for analysis as detailed in the following sections.
Histochemical Iron Staining
Histochemical staining was performed tissues from n = 5 naïve or SCI animals. Segments (~2 mm in size) of the spinal cord (2 mm rostral to the epicenter of injury site) and spleen were embedded in Optimal Cutting Temperature (OCT) compound and snap frozen in liquid nitrogen. Blocks were transferred to −80 °C for subsequent freezing. Cross-sections were cut at 10 µm on a HM 505E (Microm) cryostat and mounted onto Superfrost Plus Microscope slides (Fisher Scientific). Sections were rinsed in distilled water 3 times to remove OCT compound, followed by several rinses in 0.1 M PBS and 15 minutes in 25% hydrogen peroxide in methanol. After several more rinses in PBS, tissue was permeabilized for 10 minutes in a 0.1% Triton x-100/PBS solution and the slides were again rinsed thereafter.
For staining of ferric iron (Perls Stain, Fe3+), a 2% potassium ferrocyanide solution in 2% hydrochloric acid (HCl) solution was incubated on tissue for 30 minutes. For staining of ferrous iron (Turnbull Stain, Fe2+), a 2% potassium ferricyanide in 2% HCl solution was incubated on tissue for 30 minutes. For all spinal cord sections, DAB enhancement (Vector Laboratories, SK-4100) was facilitated in order to visualize the stain. For DAB enhancement, the slides were rinsed in 0.1 M PBS 3 times for 5 minutes each, then incubated in 0.1% Triton x-100/PBS solution for 10 minutes followed by a quick rinse in 0.1 M in PBS. The slides were then incubated with DAB enhancement solution (4 ml water with 4 drops DAB, 2 drops buffer, 3 drops nickel, 3 drops H2O2) for 1 min and then rinsed with H2O, dehydrated in an ethanol series, cleared with xylene and coverslipped with Permaslip (Alban Scientific).
IHC for ferritin
Immunohistochemical (IHC) labeling on adjacent tissue sections was performed to identify ferritin protein expression (rabbit anti-H-ferritin or rabbit anti-L-ferritin; Abcam). Briefly, sections were rinsed in 0.1 M PBS, blocked for nonspecific antigen binding using 4%BSA/0.3% Triton-100/PBS for 1 h and then incubated with primary antibodies overnight at 4 °C. The next day, sections were rinsed and incubated with secondary antibodies (goat anti-rabbit IgG; Vector) for 1 h. After rinsing, endogenous peroxidase activity was quenched using a 4:1 solution of methanol/30% hydrogen peroxide for 15 min in the dark. Sections were treated with Elite avidin– biotin enzyme complex (ABC; Vector Laboratories) for 1 h. Visualization of labeling was achieved using DAB substrates (Vector Laboratories). Sections were rinsed, dehydrated, and coverslipped with Permount (Thermo Fisher Scientific). All slides were imaged using a Zeiss Axioshop 2 Plus microscope equipped with a Sony 970 three-chip colored camera.
Magnetic Force Microscopy
Adjacent sections as that used for histochemical staining and IHC were used for magnetic force microscopy (MFM) as described earlier21. MFM studies were performed on tissues from n = 5 animals per group. The OCT embedded tissues were cryo-sectioned into 5 μm thick sections on poly-lysine coated glass coverslips, rinsed three times with ultrapure water and allowed to dry overnight in ambient air50. The glass coverslips containing the tissue sections were adhered to a metallic stub and mounted onto the JV scanner of a Multimode AFM equipped with a Nanoscope IIIa Controller (Bruker) and a reflected light module mounted over the AFM head. MFM was performed using pre-magnetized high moment (HM) MFM probes (ASYMFMHM, Asylum Research) in the tapping mode. Topographic (height) images (in main mode) and phase images (in lift mode) were recorded using the interleave scan feature of the Nanoscope software. MFM scans at a lift height (z) of 30, 40 and 50 nm were obtained from several areas corresponding to iron-rich regions as identified from adjacent sections with Perls stain. MFM phase signal was measured from regions showing a negative phase shift, using the section analysis feature of the Nanoscope software Analysis 1.5. The average roughness, Ra (the variation in phase across a region), was determined by selecting ROIs of sizes 0.03 to 0.04 μm² within a region of negative phase shift for lift height of z = 30 nm using the Nanoscope software. Ra was ascertained for at least n = 6 different regions from each animal for each tissue type. Statistical analyses was performed on n = 30 Ra values obtained from naïve vs. SCI animal groups for each tissue type.
Transmission Electron Microscopy
A subset of animals (n = 3) were utilized for TEM studies. Lesion epicenters from spinal cord injury sites (T8) were dissected into <1 mm segments, immersion-fixed overnight at 4 °C, and placed in 0.2 M cacodylate buffer for storage. Segments of the spleen tissue were processed similarly. Tissue was processed for TEM analysis, beginning with a 1-hour immersion in 1% osmium tetroxide. Samples underwent a graded ethanol dehydration series (30–100%) followed by an exchange into acetone transition solvent and subsequent infiltration series with an epoxy resin mixture (Spurr’s formulation). Following polymerization overnight in a 60 °C oven, resin blocks were semi-thin sectioned at 750 nm and stained with Methylene Blue-Azure II and Basic Fuchsin stain in order to identify regions of interest (ROI) within samples. Blocks were trimmed down to ROIs, and 40 nm thin sections were cut on a Leica Ultracut UCT ultramicrotome (Leica-Microsystems) using a Diatome Ultra knife and collected on 200 mesh copper grids. No staining after thin-sectioning was done. For conventional imaging, sections were examined on a JEOL JEM-1400 (JEOL, Peabody, MA) operating at 80 kV and digital micrographs were captured on an Olympus Veleta camera.
For analysis of ferritin particle diameter, TEM images were converted into an 8-bit format using the NIH Image J software. Regions of interest (ROIs) were selected within macrophages and processed through a bandpass filter where large structures were filtered down to 40 pixels and small structures up to 4 pixels. Ferritin iron could be easily distinguished as electron-dense particles, in filtered and thresholded ROIs. The area for each particle was assessed by using the “analyze particle” feature provided by Image J. Particle diameter was calculated by approximating the measured area to a circle. To quantify ferritin density within the macrophage cytosol, the same micrographs were analyzed using the “percent area” feature provided by Image J. Macrophage lysosomes were hand-traced and evaluated for area and pixel density as described earlier21.
Electron Energy-Loss Spectroscopy (EELS)
STEM images and electron energy-loss spectroscopy (EELS) spectra were recorded on an image Corrected Titan3TM G2 60–300 S/TEM equipped with Gatan imaging filter (GIF) system. EELS spectra were acquired with 0.025 eV/channel energy dispersion and an energy resolution of 0.175 eV. Energy resolution was measured from the full width at half maximum of the zero-loss peak (see Fig. 5A). High-loss and low-loss spectra were collected simultaneously from the same area of interest by a dual-EELS spectrometer. Spectra were energy calibrated. High-loss spectra were then background subtracted using a power-law background extrapolation. The multiple-inelastic scattering effects were removed using the Fourier-ratio technique. The percentage of Fe3+ iron present in each iron core was determined by fitting reference spectra to the Fe L-edge of the high-loss spectra. The four single valence reference mineral spectra used in the fitting procedures were: Haematite, Fe-Orthoclase, Hedenbergite and Hercynite51. Multiple Linear Least-Square (MLLS) fitting of reference spectra was applied to measure the content of Fe3+ and Fe2+ for each spectra52. At least n = 18 ferritin(iron) cores were analyzed in the lysosome and in the cytoplasm of macrophages in each sample type.
Statistical analysis
Statistical analysis was conducted on data sets obtained by analyzing images acquired from the naïve vs. SCI animals. Data analysis was carried out using GraphPad Prism 5.0. Data were compared using one-way analysis of variance followed by Bonferroni’s Multiple Comparison test. Data were considered significant when p < 0.05. All data are represented as mean + /− SD.
Data Availability
The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.
References
Rouault, T. A. Iron metabolism in the CNS: implications for neurodegenerative diseases. Nat. Rev. Neurosci. 14, 551–564 (2013).
Harrison, P. M., Fischbach, F. A., Hoy, T. G. & Haggis, G. H. Ferric Oxyhydroxide Core of Ferritin. Nature 216, 1188–1190 (1967).
Fletcher, J. & Huehns, E. R. Function of Transferrin. Nature 218, 1211–1214 (1968).
Meguro, R. et al. Nonheme-iron histochemistry for light and electron microscopy: a historical, theoretical and technical review. Archives of histology and cytology 70, 1–19 (2007).
Mikhaylova, A. et al. Detection, identification and mapping of iron anomalies in brain tissue using X-ray absorption spectroscopy. J. R. Soc. Interface 2, 33–7 (2005).
Ide-Ektessabi, A., Kawakami, T. & Watt, F. Distribution and chemical state analysis of iron in the Parkinsonian substantia nigra using synchrotron radiation micro beams. In Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms 213, 590–594 (2004).
Edelman, N. B. et al. No evidence for intracellular magnetite in putative vertebrate magnetoreceptors identified by magnetic screening. Proc. Natl. Acad. Sci. USA 112, 262–7 (2015).
Treiber, C. D. et al. Clusters of iron-rich cells in the upper beak of pigeons are macrophages not magnetosensitive neurons. Nature 484, 367–370 (2012).
Zeineh, M. M. et al. Activated iron-containing microglia in the human hippocampus identified by magnetic resonance imaging in Alzheimer disease HHS Public Access. Neurobiol Aging 36, 2483–2500 (2015).
Quintana, C., Cowley, J. M. & Marhic, C. Electron nanodiffraction and high-resolution electron microscopy studies of the structure and composition of physiological and pathological ferritin. J. Struct. Biol. 147, 166–78 (2004).
Quintana, C. et al. Study of the localization of iron, ferritin, and hemosiderin in Alzheimer’s disease hippocampus by analytical microscopy at the subcellular level. J. Struct. Biol. 153, 42–54 (2006).
Gutiérrez, L. et al. Iron speciation study in Hfe knockout mice tissues: magnetic and ultrastructural characterisation. Biochim. Biophys. Acta 1792, 541–7 (2009).
Lee, F. Y., Lee, T. S., Pan, C. C., Huang, A. L. & Chau, L. Y. Colocalization of iron and ceroid in human atherosclerotic lesions. Atherosclerosis 138, 281–8 (1998).
Kirschvink, J. L., Kobayashi-Kirschvink, A. & Woodford, B. J. Magnetite biomineralization in the human brain. Proc. Natl. Acad. Sci. USA 89, 7683–7 (1992).
Collingwood, J. F. et al. Three-dimensional tomographic imaging and characterization of iron compounds within Alzheimer’s plaque core material. J. Alzheimers. Dis. 14, 235–45 (2008).
Plascencia-Villa, G. et al. High-resolution analytical imaging and electron holography of magnetite particles in amyloid cores of Alzheimer’s disease. Sci. Rep. 6, 24873 (2016).
St Pierre, T. G. et al. Organ-specific crystalline structures of ferritin cores in beta-thalassemia/hemoglobin E. Biol. Met. 4, 162–5 (1991).
Mackle, P., Garner, C. D., Ward, R. J. & Peters, T. J. Iron K-edge absorption spectroscopic investigations of the cores of ferritin and haemosiderins. BBA - Gen. Subj. 1115, 145–150 (1991).
Meguro, R., Asano, Y., Odagiri, S., Li, C. & Iwatsuki H, S. K. The presence of ferric and ferrous iron in the nonheme iron store of resident macrophages in different tissues and organs: histochemical demonstrations by the perfusion-Perls and -Turnbull methods in the rat. Arch. Histol. Cytol. 68, 171–83 (2005).
Collingwood, J. & Dobson, J. Mapping and characterization of iron compounds in Alzheimer’s tissue. J. Alzheimers. Dis. 10, 215–22 (2006).
Blissett, A. R., Ollander, B., Penn, B., McTigue, D. M. & Agarwal, G. Magnetic mapping of iron in rodent spleen. Nanomedicine Nanotechnology, Biol. Med. 13, 977–986 (2017).
Dunn, J. R. et al. Magnetic material in the human hippocampus. Brain Res. Bull. 36, 149–53 (1995).
Kumar, P. et al. A novel approach to quantify different iron forms in ex-vivo human brain tissue. Sci. Rep. 6, 38916 (2016).
Sauerbeck, A. D. et al. Spinal cord injury causes chronic liver pathology in rats. J. Neurotrauma 32, 159–69 (2015).
Mazur, A. ROLE OF THE SPLEEN IN IRON METABOLISM. Ann. N. Y. Acad. Sci. 165, 311–319 (1969).
Sauerbeck, A., Schonberg, D. L., Laws, J. L. & McTigue, D. M. Systemic iron chelation results in limited functional and histological recovery after traumatic spinal cord injury in rats. Exp. Neurol. 248, 53–61 (2013).
Savla, M., Pandian, R. P., Kuppusamy, P. & Agarwal, G. Magnetic Force Microscopy of an Oxygen-Sensing Spin-Probe. Isr. J. Chem. 48, 33–38 (2008).
Körnig, A., Hartmann, Ma, Teichert, C., Fratzl, P. & Faivre, D. Magnetic force imaging of a chain of biogenic magnetite and Monte Carlo analysis of tip–particle interaction. J. Phys. D. Appl. Phys. 47, 235403 (2014).
Sahinkaya, F. R., Milich, L. M. & McTigue, D. M. Changes in NG2 cells and oligodendrocytes in a new model of intraspinal hemorrhage. Exp. Neurol. 255, 113–126 (2014).
Wang, X., Ong, W. & Connor, J. Increase in ferric and ferrous iron in the rat hippocampus with time after kainate-induced excitotoxic injury. Exp. Brain Res. 143, 137–148 (2002).
Cohen, L. A. et al. Serum ferritin is derived primarily from macrophages through a nonclassical secretory pathway. Blood 116, 1574–1584 (2010).
Weber, K. & Schilling, J. D. Distinct lysosome phenotypes influence inflammatory function in peritoneal and bone marrow-derived macrophages. Int. J. Inflam. 2014, (2014).
Bandyopadhyay, D., Cyphersmith, A., Zapata, J. A., Kim, Y. J. & Payne, C. K. Lysosome transport as a function of lysosome diameter. PLoS One 9, (2014).
Radisky, D. C. & Kaplan, J. Iron in cytosolic ferritin can be recycled through lysosomal degradation in human fibroblasts. Biochem. J. 336(Pt 1), 201–5 (1998).
Nairz, M., Schroll, A., Sonnweber, T. & Weiss, G. The struggle for iron - a metal at the host-pathogen interface. Cellular Microbiology 12, 1691–1702 (2010).
Church, J. S., Kigerl, K. A., Lerch, J. K., Popovich, P. G. & McTigue, D. M. TLR4 Deficiency Impairs Oligodendrocyte Formation in the Injured Spinal Cord. J. Neurosci. 36, 6352–64 (2016).
Grossman, R. G. et al. Incidence and severity of acute complications after spinal cord injury. J. Neurosurg. Spine 17, 119–28 (2012).
Salzberg, C. A. et al. A new pressure ulcer risk assessment scale for individuals with spinal cord injury. Am. J. Phys. Med. Rehabil. 75, 96–104 (1996).
Frank, C. Approach to skin ulcers in older patients. Can. Fam. Physician 50, 1653–1659 (2004).
Dobson, J. Nanoscale biogenic iron oxides and neurodegenerative disease. FEBS Letters 496, 1–5 (2001).
Nocera, T. M., Zeng, Y. & Agarwal, G. Distinguishing ferritin from apoferritin using magnetic force microscopy. Nanotechnology 25, 461001 (2014).
Ringeling, P. L. et al. Lysosomal and cytosolic ferritins A biochemical and electron-spectroscopic study. Biol. Met. 2, 114–121 (1989).
García-Prieto, A. et al. On the mineral core of ferritin-like proteins: structural and magnetic characterization. Nanoscale 8, 1088–99 (2016).
Whitnall, M. et al. Identification of nonferritin mitochondrial iron deposits in a mouse model of Friedreich ataxia. Proc. Natl. Acad. Sci. USA 109, 20590–5 (2012).
Diebel, C. E., Proksch, R., Green, C. R., Neilson, P. & Walker, M. M. Magnetite defines a vertebrate magnetoreceptor. Nature 406, 299–302 (2000).
Wang, L. et al. Evaluation of In-Situ Magnetic Signals from Iron Oxide Nanoparticle-Labeled PC12 Cells by Atomic Force Microscopy. J. Biomed. Nanotechnol. 11, 457–68 (2015).
Wood, J. C., Fassler, J. D. & Meade, T. Mimicking Liver Iron Overload Using Liposomal Ferritin Preparations. Magn. Reson. Med. 51, 607–611 (2004).
Maraloiu, V. A. et al. Multiscale investigation of USPIO nanoparticles in atherosclerotic plaques and their catabolism and storage in vivo. Nanomedicine Nanotechnology, Biol. Med. 12, 191–200 (2016).
Tamion, A. et al. Ferritin surplus in mouse spleen 14 months after intravenous injection of iron oxide nanoparticles at clinical dose. Nano Res. 9, 2398–2410 (2016).
Tonniges, J. R. et al. Collagen Fibril Ultrastructure in Mice Lacking Discoidin Domain Receptor 1. Microsc. Microanal. 22, 599–611 (2016).
Pan, Y. H. et al. 3D morphology of the human hepatic ferritin mineral core: New evidence for a subunit structure revealed by single particle analysis of HAADF-STEM images. J. Struct. Biol. 166, 22–31 (2009).
Pan, Y. et al. Electron beam damage studies of synthetic 6-line ferrihydrite and ferritin molecule cores within a human liver biopsy. Micron 37, 403–11 (2006).
Acknowledgements
The authors would like to thank Dr. Andy Brown for providing spectra of Haematite, Fe-Orthoclase, Hedenbergite and Hercynite, and Ms. Feng Qin Yin for excellent technical assistance. We are grateful to Edward Calomeni at OSU’s renal pathology EM lab for generous use of TEM equipment. This work was supported in part by the NSF CBET award 1403574 to GA and a Multidisciplinary team building grant (MTBG) grant from the Institute of Materials Research at OSU. Animal studies were supported by P30-NS045758 and NINDS RO1-NS082095 awarded to DMM.
Author information
Authors and Affiliations
Contributions
A.B. carried out animal surgery, tissue processing and TEM experiments, P.W. and A.D.S. performed histochemical stains and serum ferritin measurements, B.D. and D.M. designed and conducted EELS experiments, B.O., K.J.W. and J.S. performed MFM experiments and analysis, D.M.M. and G.A. designed the study. A.B., D.M.M. and G.A. wrote the manuscript with input from all the authors.
Corresponding authors
Ethics declarations
Competing Interests
The authors declare no competing interests.
Additional information
Publisher's note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Blissett, A.R., Deng, B., Wei, P. et al. Sub-cellular In-situ Characterization of Ferritin(iron) in a Rodent Model of Spinal Cord Injury. Sci Rep 8, 3567 (2018). https://doi.org/10.1038/s41598-018-21744-9
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/s41598-018-21744-9
This article is cited by
-
Mechanical scratch injury on differentiated motor neuron of NSC-34 cells as an in vitro model for evaluation of neuroregeneration potential of NeuroAiD II (MLC901)
In vitro models (2024)
-
Different coatings on magnetic nanoparticles dictate their degradation kinetics in vivo for 15 months after intravenous administration in mice
Journal of Nanobiotechnology (2022)
-
Protocol paper: kainic acid excitotoxicity-induced spinal cord injury paraplegia in Sprague–Dawley rats
Biological Research (2022)
-
Recent advances in metal-organic framework-based materials for anti-staphylococcus aureus infection
Nano Research (2022)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.