Abstract
Water-Soluble Chlorophyll Proteins (WSCPs) from Brassicaceae are non-photosynthetic proteins which tetramerize upon binding four chlorophyll (Chl) molecules. The bound Chls are highly photostable, despite the lack of bound carotenoids known, in Chl-containing photosynthetic proteins, to act as singlet oxygen and Chl triplet (3Chl) quenchers. Although the physiological function of WSCPs is still unclear, it is likely to be related to their biochemical stability and their resistance to photodegradation. To get insight into the origin of this photostability, the properties of the 3Chl generated in WSCPs upon illumination were investigated. We found that, unlike the excited singlet states, which are excitonic states, the triplet state is localized on a single Chl molecule. Moreover, the lifetime of the 3Chl generated in WSCPs is comparable to that observed in other Chl-containing systems and is reduced in presence of oxygen. In contrast to previous observations, we found that WSCP actually photosensitizes singlet oxygen with an efficiency comparable to that of Chl in organic solvent. We demonstrated that the observed resistance to photooxidation depends on the conformation of the phytyl moieties, which in WSCP are interposed between the rings of Chl dimers, hindering the access of singlet oxygen to the oxidizable sites of the pigments.
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Introduction
An unusual chlorophyll (Chl) binding protein in plants belonging to the Brassicaceae family was identified by Murata et al.1, 2. This protein, called Water-Soluble Chlorophyll Protein (WSCP), strikingly differs from most (bacterio)Chl-containing proteins in that it is water-soluble rather than being inserted into membranes. The only other two water-soluble (bacterio)Chl-binding proteins known so far are the Fenna-Matthews-Olson (FMO) complex3 and the Peridinin-Chlorophyll a–Protein (PCP) complex4, however, unlike WSCP, they are both involved in photosynthesis.
Recombinant WSCP has been successfully reconstituted in vitro with Chls to a tetrameric complex that biochemically and spectroscopically is equivalent to native WSCP5,6,7,8,9,10. WSCP binds four molecules of Chl a (or b) per protein tetramer, as indicated by X-ray structures11, 12 (Fig. 1). The induction of WSCP synthesis under stress conditions13,14,15,16 and its ability to protect the bound Chls against photodynamic damage5, led to the hypothesis of its functional role as a Chl scavenger10, 17. Alternatively, Boeux-Fontvieille et al.18, 19 proposed that WSCP from Arabidopsis thaliana plays a role in herbivore resistance activation during greening and in programmed cell death at least during flower development. WSCP has been shown to have protease inhibitor activity20, 21, inhibiting for instance RD21 (RESPONSIVE TO DESICCATION-21)19, a papain-like protease22, 23.
The homotetrameric structure, showing a strict 222 symmetry in the X-Ray structure of Lepidium virginicum WSCP11, forms a hydrophobic cavity in the center, occupied by four Chls. Unusually24,25,26, these are bound from their β-side, a feature that could play a role in the stabilization of the tetramer27 as well as in the capability of the apo-protein to extract Chls directly from the thylakoid proteins7 where Chls are bound mostly from their α-side. The Chls share the same symmetric relationship of the homotetramer, with two Chls at a close distance (10 Å center to center) constituting an excitonic dimer28, weakly coupled to the other dimer (ca. 20 Å center to center distance) (Fig. 1). In the “open sandwich” configuration adopted by the Chls in the protein9, the excitation of the upper energy exciton band has a transition dipole moment that exceeds that of the lower energy band by almost one order of magnitude28. This feature strikingly contrasts the properties of the excitons in strongly coupled pigments of light harvesting complexes and RCs, having most of the oscillator strength in the lower energy exciton state29. The lower dipole strength of the lower energy exciton band leads to an increase of the lifetime of the excited singlet state with strong temperature dependency30, with a concomitant increase of the ISC probability and triplet yield31. In aerobic media, Chls are known to transfer the photoexcited triplet state energy to oxygen in less than 1 μs32, resulting in the formation of highly reactive singlet oxygen (1O2). The quantum yield of such an energy transfer in solution approaches 100%33, because the natural lifetime of the Chl triplet state (3Chl) is about 1.5 ms in most solvents34. In photosynthetic Chl-binding proteins, the formation of 1O2 is generally prevented by carotenoids located close to the Chls. These carotenoids quench 3Chl through triplet-triplet energy transfer35,36,37. In addition, carotenoids are also able to deactivate 1O2 directly38, 39. Although WSCP does not contain any carotenoid, the bound Chls have been shown to be effectively resistant to photobleaching5.
So far, the unexpected photostability of WSCP-bound Chls has not been satisfactorily explained. A shielding of the Chls from oxygen by the protein scaffold forming an efficient diffusion barrier has been proposed by Schmidt et al.5, however no clear evidence for this has been provided. In principle, the presence of strongly excitonically coupled dimers could lead to triplet states unable to photosensitize 1O2, as observed in some photosynthetic systems. This mechanism has been suggested to occur in chlorosomes40, where bacteriochlorophylls form aggregated states resulting in strong π-π interactions allowing the formation of triplet excitons sufficient to lower the triplet excited state energy below that of 1O2. It is known that also the solvent or the local pigment environment may shift the triplet state energies of several chlorin-type molecules by up to 0.11 eV41, possibly below the energy of 1O2. This latter effect has been proposed to underlie the photostability of the FMO complex42.
In order to get insight into the mechanism responsible for the photostability of WSCP, we performed a systematic study using electron paramagnetic resonance (EPR) techniques to determine the properties of the photoexcited 3Chl a, flash photolysis to measure the 3Chl a lifetime, and trapping experiments to evaluate the 1O2 yield.
Results
TR- EPR and pulse ENDOR
Illumination of recombinant L. virginicum WSCP reconstituted with Chl a leads to formation of 3Chl, which can easily be detected by time resolved-EPR (TR-EPR) at low temperature (Fig. 2). The polarization pattern of the spectrum is EAEAEA (A = absorption, E = emission), confirming the ISC population mechanism of the 3Chl. The population probabilities are more similar to those reported for 3Chl a in 2-Methyltetrahydrofuran (MTHF)43, rather than for 3Chl a in light-harvesting chlorophyll-binding proteins44,45,46,47,48.
Simulation of the spectrum (Fig. 2) has been obtained with the values reported in Table 1. The zero-field splitting (ZFS) parameters used in the simulation to reproduce the TR-EPR spectrum were similar to those reported for 3Chl a in MTHF and 3P680 in photosystem II particles49. The scheme of the Zeeman splitting of the triplet sublevels typical of chlorins, at the three canonical orientations of the triplet state (i.e. magnetic field parallel to the ZFS tensor axes X, Y, or Z)50, is reported in Fig. S1
The similarity of the D value of 3Chl a in WSCP with that measured in MTHF49, suggests a localization of the triplet state on only one of the two Chls constituting the Chl dimers bound to the protein, as observed for other Chl dimers in photosynthetic reaction centers of plants49, 51. However, a triplet state delocalization among Chls could still occur even in the absence of significant changes in the ZFS parameters with respect to those of the corresponding monomer, as recently observed in synthetic conjugated multimeric porphyrin systems52. On the contrary, electron-nucleus hyperfine coupling (hfc) constants, being directly related to the spin density distribution, strictly depend on the triplet delocalization. Indeed, transient Electron Nuclear Double Resonance (ENDOR) and pulsed ENDOR experiments performed on 3P680 clearly was crucial to demonstrating that the primary donor triplet in photosystem II is localized on a monomeric Chl species49, 51. Thus, we measured the proton hyperfine couplings on 3Chl a by means of pulse ENDOR spectroscopy.
Davies ENDOR spectra in WSCP were collected at field positions corresponding to orientations of ZFS axes of 3Chl parallel to the static field B0, yielding single crystal-like ENDOR spectra, from which the projections of the hfc tensors on the ZFS axis system can be extracted. For transitions connecting the ms = 0 and ms = + 1 levels, positive hfcs give rise to ENDOR signals at frequencies lower than proton Larmor frequency (νH), while negative hfcs are visible at higher frequencies with respect to νH (see black lines in Fig. 3A).
It is known that Davies pulse sequence does not allow to resolve signals with small hfcs. On the other hand, Mims ENDOR is particularly suitable for measuring small hfcs, but, since the spectra are intrinsically affected by blind spots, averaging at different τ values is required, making the experiment time consuming. Therefore, we performed Mims experiments only in a narrow frequency interval around νH (purple line in Fig. 3A) sufficient to obtain a complete set of the smaller hfcs.
The spectra were highly structured and well resolved, showing sharper peaks compared to the pulse ENDOR spectra of 3Chl a in MTHF reported before49. This is likely due to the lower inhomogeneous broadening experienced in the protein medium compared to the frozen glassy organic solution (MTHF). A similar effect was previously observed for the ENDOR spectrum of the primary donor 3P68049.
In the negative region of the spectra two main narrow peaks are distinguishable at each orientation selected by the field position, which can be attributed to protons belonging to rotating methyl groups, on the basis of the sign of the hfcs and on their small anisotropy. In particular, the highest hfc (aiso = + 10.7 MHz) is almost coincident with the value observed in the ENDOR spectrum of 3P680 (aiso = + 10.6 MHz), a monomeric 3Chl a 49, and assigned to methyl 12 (nomenclature as reported in Fig. 3B).
The other intense peak, with lower hfc values (aiso + 4.7 MHz), may be assigned to methyl 2 on the basis of the hfcs reported for the Chl a cation53 and anion54 at this proton position, taking into account that, in the Hückel approximation, the hfc for a nucleus of a triplet state is expected to be the average of those observed in the respective cation (singly occupied HOMO) and anion (singly occupied LUMO) radicals, since in the triplet state the HOMO and LUMO are both singly occupied49. It is worth noting that this is the first time that the transition belonging to methyl 2 protons is clearly detected in a 3Chl a ENDOR spectrum. Methyl at position 7 is expected to give smaller hfcs, on the basis of the corresponding values measured for the cation and the anion radicals, which likely correspond to the less intense transitions visible in the Mims spectra (aiso = + 1.1 MHz), as reported in Table 2.
In the positive part of the spectra three signals are present which can be assigned to three α protons, due to their sign and large anisotropy. Comparison with the previously published couplings relative to 3P680 and 3Chl a in frozen organic solvent, allows us to assign the observed transitions to the specific protons in the structure (Table 2)49. The small hfcs, which are well resolved in our Mims spectra, were neither assigned nor detected in previous pulse ENDOR studies, and will require a further computational analysis to reach a reliable and complete assignment.
The 3Chl a Z-axis is perpendicular to the molecular plane, and is expected to be also a principal axis of the observed hfc tensors for the -CH3 and α protons. Hence, changes in the spin density distribution due to delocalization of the triplet state between different Chls, should be directly reflected in the AZ hfc values of the protons. Therefore, the absence of any reduction of the hfcs compared to 3P680 and 3Chl a in vitro clearly indicate that in WSCP the triplet state is localized on a single Chl molecule without any significant delocalization on the other Chls bound to the same protein unit.
Flash photolysis
The characterization of the triplet state with EPR techniques ruled out the possibility of a photoprotective mechanism based on delocalization of the triplet state. Another important property of the triplet state that could be related to the slow photobleaching is the triplet lifetime. Since the intensity of the EPR signal depends mainly on spin relaxation processes and the 3Chl lifetime is usually not directly accessible by TR-EPR measurements55, we measured the 3Chl a lifetime by means of flash photolysis.
From the inspection of the flash photolysis spectra reported in Fig. 4, it is clear that the Chl ground state is depleted in the ms time range under anoxic conditions at least for a large part of all molecules, while, in oxic conditions, this time shortens to less than 10 µs (the limit of the time response of the setup). After subtraction of the lamp profile from the anoxic time trace, a lifetime of 1.35 ms and 40% amplitude for recovery of the ground state and then for the 3Chl a in WSCP was obtained, a value which is typical for 3Chl a in both organic solvent and proteins34. In addition, a further fast decay component near the resolution limit (10–20 µs) with 60% amplitude is found that results from spectral variations of the lamp profile which make it difficult to achieve a complete subtraction from the measured data and represent ground state depletion not correlated with inter system crossing and triplet formation. Hence an intrinsic shortening of the lifetime of the triplet state is not observed and cannot be invoked for explaining the photostability of WSCP. On the other hand the lifetime resulted to be very sensitive to the presence of O2, ruling out the hypothesis that the protein scaffold is shielding 3Chl a from interaction with oxygen, in agreement with our recent findings on Brassica oleracea WSCP27.
Schmidt et al.5 previously reported that light-induced 1O2 formation in WSCP was significantly reduced compared to that of free Chls in solution. This would imply that the shortening of the lifetime of 3Chl that we observed in the presence of O2 should take place without generating the oxidative species 1O2. In order to verify this possibility, we measured the photo-induced 1O2 yield in our system.
1O2 and photobleaching quantification
The photoproduction of 1O2 was measured using the fluorescent probe Singlet Oxygen Sensor Green® (SOSG). This sensor selectively reacts with 1O2, leading to an endo-peroxidized product with a highly increased fluorescence quantum yield. The measurements of 1O2 were accompanied by photobleaching measurements, performed on the same samples, by means of Chl fluorescence detection.
A remarkable photostability of WSCP was observed, as highlighted by the comparison with the major light harvesting complex (LHCII) of the photosynthetic apparatus in plants (Fig. 5A). The two Chl-binding complexes display comparable photobleaching, largely reduced in comparison to a reference sample containing free Chl a in octyl glucoside (OG) micelles. Thus, WSCP and LHCII exhibit a similar light tolerance although WSCP does not contain any carotenoids whereas LHCII binds four of them56.
Surprisingly and in marked contrast to the previous work of Schmidt et al.5 mentioned above, we detected an intense 1O2 production of WSCP (Fig. 5B), that reached a plateau only due to the consumption of the SOSG as highlighted by a control experiment in which upon addition of fresh SOSG, the fluorescence signal started again to grow upon illumination (data not shown). In comparison, LHCII exhibited a far smaller 1O2 production. A reference sample with unbound Chl a in OG solution displayed, under the same experimental conditions, an unexpectedly low 1O2 evolution. However, the reason for this behavior can be attributed to the concomitant high degree of photobleaching: due to the fast reaction with 1O2, the number of excitable Chls quickly decreases, and consequently less and less 1O2 is photosensitized at increasing time intervals.
Since these results, showing a high 1O2 yield in WSCP, were in contrast with those previously published, we performed also control spin-trapping experiments by Continuous-Wave (CW) EPR spectroscopy. When working in the same experimental conditions described by Schmidt et al.5, that is in the presence of n-dodecyl-β-maltoside (DM) (Fig. S2A), we also measured a low 1O2 yield. The different quantification of 1O2 measured by SOSG and spin probe methods may be related to the different repartition coefficients of the probes between the micellar and the aqueous phases. SOSG, which is a hydrophilic molecule, efficiently reacts with the 1O2 photosensitized by WSCP, whereas TEMP (2,2,6,6-Tetra-methyl-piperidine) is expected to be more concentrated in the micellar phase, in analogy with TEMPO (2,2,6,6-Tetra-methyl-piperidin-1-oxyl)57. Accordingly, we obtained a strong increase of the EPR signal of the spin trap upon removal of the DM from the WSCP solution (Fig. S2A), confirming that TEMP is sequestered away from the aqueous phase in the presence of the micelles. Therefore the underestimate of the 1O2 photosensitization in the previous report5 is due to the interfering presence of DM micelles.
Control experiments with free Chl a dissolved in an organic phase (ethanol) rather than in a micellar phase showed a 20% lowering (as estimated by double integration of the EPR signal) of the signal of the 1O2 spin trap (Fig. S2B). The decrease of the signal under this condition can be attributed to the fact that in homogeneous solution the increase in the local concentration, generated in micelles by the confinement of Chls and TEMP, is lost, and the probability of encounter between TEMP and 1O2 is consequently diminished.
In conclusion, our experimental approach based on two different methods for determining the 1O2 production led to the conclusion that 1O2 production in WSCP is not suppressed but is of the same order as that of free Chl and larger than that observed in LHCII (Fig. 5B and C), although a quantitative comparison between the two proteins is difficult, due to the fact that LHCII is a membrane protein while WSCP is water-soluble.
Prompted by the results on the triplet state characterization and singlet oxygen formation, we looked at the protein environment of the Chls, searching for a possible explanation of the scarce reactivity of the pigments towards 1O2. From the inspection of the crystallographic structure11, it appears that two tryptophans (Trp) are in close contact to the Chl macrocycle (namely W90 and W154). A possible role of Trp in photoprotection of photosynthetic proteins has already been hypothesized58, because of their high reactivity with 1O2 59, 60. We mutated both of them to phenylalanine (Phe) residues, chosen because of the similarity of the side chains and the reduced reactivity with 1O2 reported for Phe61. The mutants were successfully reconstituted with Chl a, obtaining complexes with unchanged absorption and dichroism spectra in comparison to the wild type (see Fig. S3). Photobleaching of the mutants was very similar to that of the wild type, with a comparable loss of Chl a fluorescence (see Fig. S4) clearly indicating that neither of the Trp is involved in the photoprotection of the bound Chls, at least not by reaction with 1O2.
In order to study a possible photoprotective role of the phytyl chains of the bound Chls, which are interposed between the Chl planes, WSCP was reconstituted with chlorophyllide a (Chlide a), i.e. the phytyl-free analog of Chl a. The reconstituted pigment-protein complex was obtained in high yield and the purification via size exclusion chromatography showed that it was comparable in the size of the oligomers to the Chl a analog (see Fig. S5). Thus, although Chlide-containing WSCP is less stable than the Chl complex, the phytyls are not strictly required for the tetramerization of WSCP, in contrast to a previous report5.
It is well known that Chlide presents spectroscopic characteristics analogous to Chl, although some small differences have been reported62. The Chl a and Chlide a reconstituted complexes exhibited similar optical properties, as evident from the absorption and CD spectra (Fig. S6). Both Chl a and Chlide a upon binding to WSCP undergo a change in their absorption spectra compared to that of the pigments in organic solvents (see Fig. S7). The spectral similarity strongly suggests that the Chlides bound to WSCP are excitonically coupled in the same open sandwich configuration adopted by Chls. Interestingly, Chlide a WSCP showed a severe photobleaching (Fig. 5A), with an 80% loss of fluorescence after 1 h illumination. This is much higher than the photodegradation of Chl a WSCP under the same conditions (Fig. 5A), despite the similar spectral properties and although the 1O2 production was comparable between Chl a- and Chlide a-containing WSCP, as revealed by SOSG quantification (Fig. 5B).
Discussion
In the present study, we used magnetic resonance techniques to characterize the 3Chl a populated in WSCPs under illumination. Our experimental results provide clear evidence for the triplet state to be localized on a single Chl molecule among the four interacting pigments belonging to one protein tetramer. This was demonstrated by the large values of the hfcs determined by ENDOR spectroscopy, which were very similar to those reported for monomeric 3Chl a in frozen organic solution and for the primary donor 3P68049. On the basis of these results we conclude that the photoprotective properties of the WSCP complex cannot be derived from a delocalization of the triplet state.
In marked contrast to previous findings5, we measured an intense photoproduction of 1O2 in WSCP complexes. The method used in the study of Schmidt et al.5, performed on Brassica oleracea WSCP, led to an underestimation of 1O2 due to the presence of detergent in the solution and to the limited solubility in water of the TEMP spin-trap. By modifying the experimental conditions we obtained spin-trapping results in agreement with SOSG quantification. Thus, the remarkable photostability of WSCP5, which is comparable to the one displayed by Chl-protein complexes in which the 1O2 production is efficiently avoided by the photoprotective role of carotenoids, has to rely on the capability of the system to resist to bleaching after 1O2 production. We have ruled out a possible involvement of two conserved tryptophans which are in van der Waals contact with the chlorin planes of the Chls.
The experiments performed on WSCP reconstituted with Chlide a clearly indicate that the presence of the Chl phytyls is required for the photostability of the system. A photoprotective action of the phytyls through a direct reaction with 1O2, i.e. acting as oxidizable species63, can be excluded, as this would have led to a photobleaching curve characterized by a change in slope, with a faster loss in fluorescence once all the phytyls are consumed by the reaction. Instead the profile of the Chl fluorescence keeps its mono-exponential decay unchanged also for long illumination times (see Fig. S8). The phytyls are intertwined between the Chl pairs constituting the open sandwich dimers (see Fig. 1). Interestingly this configuration brings them in van der Waals contact with the methine carbon atom of the Chl ring (number 20 in Fig. 3B, colored in yellow in Fig. 6) that is known to be the most susceptible site for reaction with 1O2 in the photobleaching of related Chls (Zn-Chl a 64 and BChl c 65).
The conformation of the phytyls seems to avoid an interaction of the methine 20 with the solvent (see Fig. 6B and C). The phytyls are also in close contact with the central Mg ions of the Chls and shield the Mg from the solvent. In a study conducted on bacteriochlorophylls (BChl) it has been proposed that interactions between oxygen and Mg increase the residence time of singlet oxygen near the BChls and, thus, lowers the photostability of the pigments66. Consequently, phytyls in WSCP may exert their photoprotective role also by masking the Mg ions rather than methine 20. It is likely that several structural components of WSCP contribute to the protection of the Chl moiety against access of singlet oxygen although clearly the phytyls play the most significant role. At the same time a small portion of the ring remains accessible (see Fig. 6B), which explains why the molecular oxygen can exchange triplet energy with 3Chl populating the 1O2.
It is worth noting that a photoprotective mechanism like this, with the reactive portions of the Chl macrocycle sterically shielded from the detrimental interaction with 1O2, has never been reported before in a protein system, but it is similar to that found by Bonchio et al.67 in a porphyrin-cyclodextrin supramolecular complex68, 69. These authors found that the encapsulation of the porphyrins inside a cyclodextrin matrix efficiently photoprotects the porphyrins from photobleaching, while preserving the capability of 1O2 sensitization. The available structural data of that system68,69,70 suggested that two cyclodextrins shield the methine carbon atoms avoiding their reaction with the photoproduced 1O2.
With regard to possible biological roles of WSCP, the protein has been proposed by Takahashi et al. as a Chl scavenger protecting cells from photodynamic damage by taking up unbound Chls and keeping them from producing 1O2 10, 17. This hypothesis is not consistent with the present finding of WSCP as an active 1O2 producer. The remarkable photostability of the complex would render the Chl even more dangerous to the cell, since its elongated lifespan would lead to an even increased production of 1O2 over time.
The present observations suggest a different biological role of WSCP producing 1O2 as a biological signal for pathogen attack. Since in green tissues WSCP apoproteins are not located in chloroplasts but in ER bodies10, 17, 71, organelles involved in plant defense against pathogens and/or herbivores (for reviews see refs 72 and 73), WSCP complex formation can only occur upon cell disruption including organelle dismantling. WSCP apoproteins set free from the ER bodies would form tetrameric WSCP for instance by extracting Chls from thylakoid membranes7. In the presence of oxygen and light, these WSCPs would then produce 1O2.
It has been shown that the release of 1O2 triggers the activation of distinct stress response genes in Arabidopsis that are different from those activated by other reactive oxygen species like H2O2 or O2 ∙− 74, 75. Hence 1O2 can function as a specific signal molecule inducing a specific set of plant stress responses76. The 1O2-triggered signaling pathways (for a review see ref. 77) are considered to result, depending on the level of produced 1O2, either in an acclimation response or in triggering programmed cell death programs (for a review see ref. 78). Moreover, certain hydroxy fatty acids due to non-enzymatic lipid oxidation by 1O2 were found to accumulate in A. thaliana leaves upon pathogen attack79, 80. Interestingly, application of hydroxy fatty acids as well as an induced production of 1O2 (by a photosensitizer) led to a strong accumulation of callose in leaves of A. thaliana 81, which has been shown to be a frequent response of plant cells towards pathogen attack82.
Consistently with the proposed role of WSCP as a producer of a 1O2 signal upon cell damage, the expression of the WSCP apoprotein is stimulated by pathogen attack83, 84 among other stress situations13,14,15,16, 18, 21, 85, 86. At extreme levels of 1O2, genetically controlled cell death responses are masked by genetically independent cell death reactions caused by the direct cytotoxicity of elevated levels of 1O2 87 leading to the so-called accidental cell death78. In line with the latter notion, elevated levels of 1O2 produced by WSCP catalysis may even serve as an efficient weapon, directly damaging those pathogens that caused the formation of WSCP in the first place.
Another hypothesized biological role of WSCP is based on the observation that all Brassicaceaen WSCPs isolated so far possess a signature sequence of Künitz-type protease inhibitors (KTI)6, 7, 10, 15, 17. While some WSCPs have been reported to show inhibitory activity against proteases19, 21, 88, others did not15, 89. In etiolated apical hook tissue, Reinbothe and co-workers18, 19, 90 have recently identified a complex consisting of A. thaliana WSCP apoprotein interacting with RD21, a papain-like cystein protease involved in stress response and defense22, 23, 91,92,93. This interaction inhibits the cysteine protease activity of RD2119. Upon exposure to light, the RD21-WSCP complex dissociates, presumably due to pigment uptake of the WSCP apoprotein18, 90 suggesting a Chl/Chlide driven activation of the protease activity.
Clearly, WSCP functioning as a protease inhibitor de-activated by Chl binding would benefit from the photostability described in the present work. If the WSCP-bound Chls were rapidly photodegraded upon illumination, this would presumably unmask the protease inhibitor activity of the apoprotein again. It is worth noting that even at long illumination times, with a concomitant photodamage of some of the Chls bound within a tetramer, no WSCP complex degradation into monomers occurs (see Fig. S9). It should be noted that the two hypothesized WSCP activities in the plant defense, WSCP acting as a Chl-regulated protease inhibitor and WSCP acting as a producer of 1O2 as a signal for cell disruption, are fully compatible with one another and can be conceived as being performed in parallel.
Conclusion
We have shown that Chls in WSCP efficiently produce 1O2 upon illumination but even so undergo little photooxidation. Our study solves the riddle of this photostability. Photoprotection requires the presence of the phytyl moieties of the WSCP-bound Chls, either by protecting those sites of the Chl macrocycle that are mostly susceptible to the 1O2 attack or by limiting the residence time of 1O2 close to them.
We propose that the photostability of the Chls bound to WSCP is required to allow playing the cell function, since Chls are needed to initially form the tetrameric structure of the protein, which in turn is instrumental for “sensing” and signaling the change in cellular conditions which leads to Chl release.
The quite simple mechanism of how the WSCP-bound Chls are photoprotected may inspire molecular design in the fields of photodynamic therapy or photocatalysis.
Material and Methods
Sample preparation
Chl a was isolated and purified from pea plants (Pisum sativum) according to Booth and Paulsen94. Chlide a was obtained using recombinant chlorophyllase from wheat (Triticum aestivum). Expression and purification of recombinant chlorophyllase was carried out following the protocol described earlier95. Chlorophyllase assay used for obtaining Chlide a is based on the method of Fang et al.96 with a few variations of the protocol. The reaction mixture containing 20 mM sodium phosphate (pH 7.8), 0.5 mg/ml chlorophyllase, 1 mM ascorbate, 5 mM MgCl2, 50% (v/v) acetone and 1.25 mg/ml Chl a was incubated for 4 h at 37 °C and 80 rpm. After adjusting to 70% acetone, the reaction mixture was centrifuged (10 min, 4 °C, 10,000 × g) and the supernatant subjected to Sep-Pak® cartridges (Plus tC18, Waters) for purification via reversed phase chromatography. Chlide a was eluted by washing the cartridges with 4 ml of 70% acetone. After quantification via analytical HPLC, pigments were dried and stored under N2 atmosphere at −20 °C.
Pet24b plasmid with a gene encoding for a recombinant WSCP protein from L. virginicum possessing a C-terminal hexahistidyl tag17 was kindly provided by the group of Prof. Satoh (Toho University, Japan). The plasmid was transformed in Escherichia coli (BL21). Recombinant apo-protein expression and purification was carried out as described previously27 except the bacteria growth was carried out at 37 °C and kanamycin was used as the selection antibiotics. The purified apoprotein was reconstituted with a three-fold molar excess of either Chl a or Chlide a. Pigments were dissolved in Triton™ X-114 (TX-114) and added dropwise to the protein solution containing 20 mM sodium phosphate (pH 7.8) and 5 mM β-mercaptoethanol yielding a final concentration of TX-114 of 2% (v/v). After incubation for 30 min at 4 °C and 30 rpm (Intelli-Mixer, neoLab), phase separation was induced by increasing the temperature to 40 °C followed by a 5 min incubation step. The aqueous phase harboring the reconstituted WSCP complexes was collected after centrifugation (2 min, at room temperature, RT, 14,000 × g) and applied to a Superose™ 12 10/300 GL prepacked column (GE Healthcare) equilibrated with 20 mM sodium phosphate (pH 7.8) and operated by a BioRad NGC system at RT for further purification via size-exclusion chromatography (SEC). Peak fractions containing tetrameric WSCP were analyzed via absorption and circular dichroism (CD) spectroscopy. Recombinant LHCII was reconstituted and purified as described earlier97, 98. After sucrose density gradient purification, trimeric LHCII was collected and adjusted to 20 mM sodium phosphate (pH 7.8) + 0.2% (w/v) DM. LHCII complex integrity and functionality was verified by applying absorption, CD and fluorescence spectroscopy.
TR-EPR and pulse ENDOR
Samples were concentrated to a Chl concentration of 700 μg/ml. Oxygen was removed by flushing nitrogen in the EPR sample tube before freezing. Residual oxygen was removed exploiting the glucose/glucose oxidase/catalase system99. Glycerol, previously degassed by several cycles of freezing and pumping, was added (60% v/v) just before freezing to obtain a transparent matrix.
X-band TR-EPR experiments were performed on a modified Bruker ER200D spectrometer with an extended detection bandwidth (6 MHz), allowing a response time of about 150 ns. Laser excitation at 532 nm (5 mJ per pulse and repetition rate of 50 Hz) was provided by the second harmonic of a Nd:YAG laser (Quantel Brilliant) in a high-Q cylindrical TE011 resonant cavity. The experiments were carried out with a microwave power in the cavity of 2 mW. The temperature was controlled with a nitrogen flow cryostat. The signal was recorded with a LeCroy LT344 digital oscilloscope, triggered by the laser pulse. The spectra were recorded averaging 400 transient signals at each field position. To eliminate the laser background signal, transients accumulated under off-resonance field conditions were subtracted from those on resonance. Simulations of the powder spin-polarized triplet TR-EPR spectra were performed using a program written in MatLab®, with the aid of the Easyspin routine (ver. 5.1.9)100, based on the full diagonalization of the triplet state spin Hamiltonian, including the Zeeman and magnetic dipole–dipole interactions, considering a powder distribution of molecular orientations with respect to the magnetic field direction44. Input parameters are the triplet state sublevel populations, the zero-field splitting (ZFS) parameters, the linewidth, and the isotropic g value.
X-Band Pulse ENDOR experiments were performed on a Bruker Elexsys E580 spectrometer. Laser excitation at 532 nm (10 mJ per pulse and repetition rate of 10 Hz) was provided by the second harmonic of a Nd:YAG laser (Quantel Brilliant) in a dielectric cavity. The temperature was controlled with a Helium cryostat (Oxford CF935) driven by a temperature controller (Oxford ITC503).
Davies-ENDOR experiments were performed using the microwave pulse sequence (flash-π-T-π/2-τ-π-τ-echo) with an inversion π pulse of 200 ns, T of 8 μs and a RF pulse of 6 μs. The detection sequence was similar to the field-swept ESE experiment but with τ of 300 ns.
Mims ENDOR experiments were recorded using the microwave pulse sequence (flash-π/2-τ-π/2-T-π/2-echo), with 16 ns pulse duration, in conjunction with an RF pulse of 6 μs duration, starting 0.8 μs after the second microwave pulse. The delay τ was variable, and the time T was 8 μs to accommodate the RF pulse. Mims ENDOR spectra were recorded at different τ values (from 160 to 240 ns) and added together to eliminate τ-dependent blind spots. Pulse ENDOR spectra were accumulated for ≈15 h.
Flash photolysis
Flash-induced absorbance difference spectra on the µs to ms time domain were recorded at RT using a laboratory-built flash spectrometer as described by Gerken et al.101. The samples (2 ml; adjusted to an OD of 0.5 at 680 nm corresponding to around 7.5 µg Chls/mg) were placed in a 1 cm cuvette and excited with saturating flashes of ~20 µs in duration from a Xe flash lamp filtered by colored glass with transmission at 680 nm (model CS96–4 from Corning). Measuring light from a 55 W tungsten halogen lamp was passed through a monochromator with a spectral bandwidth of 3 nm, the optical cuvette and an interference bandpassfilter at 430 nm (AL 431) in front of a photomultiplier (EMI 9558BQ) coupled to a transient recorder (Tektronix TDS320) monitoring the bleaching of the Chl ground state absorption upon excitation. The time course of the absorbance changes in oxygen atmosphere was fitted to a biexponential decay including offset using Origin 8.0 ® software, after subtraction of the lamp profile. For measurements under anoxic conditions, oxygen was removed by using the glucose/glucose oxidase/catalase system99.
Photobleaching
Photobleaching was measured by quantitating the loss in fluorescence intensity upon illumination with white light at 500 µmol of photons m−2 s−1. As a control, free Chl a was dissolved in a 20 mM sodium phosphate (pH 7.8) with 2% (w/v) OG. All samples were adjusted to a Chl a extinction of 0.005 (around 0.07 µg Chls/ml) at Qy maximum in either 20 mM sodium phosphate pH 7.8 (WSCP) or 20 mM sodium phosphate (pH 7.8) + 0.2% (w/v) DM (LHCII).
After excitation at 410 nm, Chl a emission (F) detected at the maximum of the emission spectrum (at 667, 674, 678 and 674 nm for Chl a WSCP, Chlide a WSCP, LHCII and Chl a in 2% (w/v) OG, respectively) was compared to the initial emission before illumination (F0). F/F0 was plotted against illumination time. The measurements were performed on a FluoroMax-2 instrument (Jobin Yvon-Spex) at RT using a cuvette with the dimensions of 5 × 5 mm and the following parameters: slits 1 nm, increment 1 nm, integration time 1 s.
Singlet oxygen quantification
Quantification of 1O2 production was performed with SOSG (Molecular Probes, Eugene). SOSG is a fluorescent probe highly selective for 1O2 that increases its 530 nm emission band upon reaction with this species102. The different samples were diluted to the same integrated absorption area in the wavelength range 590–750 nm (around 2.5 μg Chls/ml) in the presence of 2 μM SOSG and 2% (v/v) methanol, and illuminated with red light (λ ≥ 590 nm) at 500 µmol of photons m−2 s−1 under stirring. Fluorescence yield of SOSG (λex 480 nm, λem 530 nm, slits 0.5 nm, increment 1 nm, integration time 1 s, cuvette 1 × 1 cm) was determined after light treatment in order to quantify 1O2-dependent fluorescence increase.
Spin-trapping assays of 1O2 were also performed following the procedure described in Schmidt et al.5. All the samples were prepared in 20 mM sodium phosphate (pH 7.8) with a Chl concentration of 1 μg/mL, 100 mM TEMP, and 3.5% methanol (v/v). Samples were illuminated for one hour with white light at 150 µmol of photons m−2 s−1 on a water bath at 20 °C. CW EPR spectra of the samples were measured before and after exposure to light with a Bruker Elexsys E580 spectrometer. X-band spectra were recorded at 298 K with a modulation frequency of 100 kHz, a modulation amplitude of 0.2 mT, a microwave frequency 9.6 GHz, and a microwave power of 3.76 mW. Spin-trap concentrations were determined by performing double integration of the EPR signals.
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Acknowledgements
This work has been funded by a grant from the Deutsche Forschungsgemeinschaft to H.P. (Pa 324/10-1), and by a grant from the Italian Ministry for University and Research to M.D.V. (PRIN2010 prot.2010FM38P_004). A.A. gratefully acknowledges the Ing. Aldo Gini foundation for supporting his stay in Germany with a travel grant.
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H.P. and D.C. designed the research, A.A. and D.M.P. prepared the WSCP and LHCII samples and performed and analysed the fluorescence measurements, A.A., M.A., and M.D.V. performed and analysed the TR-EPR and ENDOR measurements, F.J.S. performed and analysed the flash photolysis measurements, A.A. performed and analysed the spin-trapping measurements, A.A., D.M.P., H.P., and D.C. wrote the paper.
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Agostini, A., Palm, D.M., Schmitt, FJ. et al. An unusual role for the phytyl chains in the photoprotection of the chlorophylls bound to Water-Soluble Chlorophyll-binding Proteins. Sci Rep 7, 7504 (2017). https://doi.org/10.1038/s41598-017-07874-6
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DOI: https://doi.org/10.1038/s41598-017-07874-6
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