Brief Communications Arising | Published:

Inter-homologue repair in fertilized human eggs?

Naturevolume 560pagesE5E7 (2018) | Download Citation

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1.

    Ma, H. et al. Correction of a pathogenic gene mutation in human embryos. Nature 548, 413–419 (2017).

  2. 2.

    Shin, H. Y. et al. CRISPR/Cas9 targeting events cause complex deletions and insertions at 17 sites in the mouse genome. Nat. Commun. 8, 15464 (2017).

  3. 3.

    Whitworth, K. M. et al. Use of the CRISPR/Cas9 system to produce genetically engineered pigs from in vitro-derived oocytes and embryos. Biol. Reprod. 91, 78 (2014).

  4. 4.

    Parikh, B. A., Beckman, D. L., Patel, S. J., White, J. M. & Yokoyama, W. M. Detailed phenotypic and molecular analyses of genetically modified mice generated by CRISPR-Cas9-mediated editing. PLoS One 10, e0116484 (2015).

  5. 5.

    Kosicki, M., Tomberg, K. & Bradley, A. Repair of double-strand breaks induced by CRISPR–Cas9 leads to large deletions and complex rearrangements. Nat. Biotechnol. https://doi.org/10.1038/nbt.4192 (2018).

  6. 6.

    Sultan, K. M., Munné, S., Palermo, G. D., Alikani, M. & Cohen, J. Chromosomal status of uni-pronuclear human zygotes following in-vitro fertilization and intracytoplasmic sperm injection. Hum. Reprod. 10, 132–136 (1995).

  7. 7.

    Cole, F. et al. Mouse tetrad analysis provides insights into recombination mechanisms and hotspot evolutionary dynamics. Nat. Genet. 46, 1072–1080 (2014).

  8. 8.

    Jeffreys, A. J. & May, C. A. Intense and highly localized gene conversion activity in human meiotic crossover hot spots. Nat. Genet. 36, 151–156 (2004).

  9. 9.

    Kauppi, L. et al. Numerical constraints and feedback control of double-strand breaks in mouse meiosis. Genes Dev. 27, 873–886 (2013).

  10. 10.

    Baker, T. G. A quantitative and cytological study of germ cells in human ovaries. Proc. R. Soc. Lond. B 158, 417–433 (1963).

  11. 11.

    Stark, J. M. & Jasin, M. Extensive loss of heterozygosity is suppressed during homologous repair of chromosomal breaks. Mol. Cell. Biol. 23, 733–743 (2003).

  12. 12.

    Kattera, S. & Chen, C. Normal birth after microsurgical enucleation of tripronuclear human zygotes: case report. Hum. Reprod. 18, 1319–1322 (2003).

  13. 13.

    Egli, D., Rosains, J., Birkhoff, G. & Eggan, K. Developmental reprogramming after chromosome transfer into mitotic mouse zygotes. Nature 447, 679–685 (2007).

  14. 14.

    Egli, D. et al. Reprogramming within hours following nuclear transfer into mouse but not human zygotes. Nature Commun. 2, 488 (2011).

  15. 15.

    Reichmann, J. et al. Dual-spindle formation in zygotes keeps parental genomes apart in early mammalian embryos. Science  361, 189–193 (2018).

  16. 16.

    Suzuki, T., Asami, M. & Perry, A. C. Asymmetric parental genome engineering by Cas9 during mouse meiotic exit. Sci. Rep.  4, 7621 (2014).

Download references

Author information

Affiliations

  1. Department of Obstetrics and Gynecology and Department of Pediatrics, Columbia University, New York, NY, USA

    • Dieter Egli
  2. Graduate Program, Department of Physiology and Cellular Biophysics, Columbia University, New York, NY, USA

    • Michael V. Zuccaro
  3. Wellcome Sanger Institute, Hinxton, Cambridge, UK

    • Michael Kosicki
    •  & Allan Bradley
  4. Department of Genetics, Harvard Medical School, Boston, MA, USA

    • George M. Church
  5. Developmental Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA

    • Maria Jasin

Authors

  1. Search for Dieter Egli in:

  2. Search for Michael V. Zuccaro in:

  3. Search for Michael Kosicki in:

  4. Search for George M. Church in:

  5. Search for Allan Bradley in:

  6. Search for Maria Jasin in:

Contributions

D.E. and M.J. wrote the paper with contributions from M.K., A.B., M.Z. and G.M.C. M.Z. and D.E. performed ICSI and imaging of eggs. D.E. is a NYSCF-Robertson investigator.

Competing interests

G.M.C. is an advisor at Veritas Genetics and Editas Medicine. A comprehensive list of G.M.C.’s technology transfer and advisory roles is provided here http://arep.med.harvard.edu/gmc/tech.html. Other authors declare no competing interests.

Corresponding authors

Correspondence to Dieter Egli or Maria Jasin.

Supplementary information

About this article

Publication history

Received

Accepted

Published

DOI

https://doi.org/10.1038/s41586-018-0379-5

Further reading

  • 1.

    Did CRISPR really fix a genetic mutation in these human embryos?

    • Ewen Callaway

    Nature (2018)

  • 2.

    Ma et al. reply

    • Hong Ma
    • , Nuria Marti-Gutierrez
    • , Sang-Wook Park
    • , Jun Wu
    • , Tomonari Hayama
    • , Hayley Darby
    • , Crystal Van Dyken
    • , Ying Li
    • , Amy Koski
    • , Dan Liang
    • , Keiichiro Suzuki
    • , Ying Gu
    • , Jianhui Gong
    • , Xun Xu
    • , Riffat Ahmed
    • , Yeonmi Lee
    • , Eunju Kang
    • , Dongmei Ji
    • , A-Reum Park
    • , Daesik Kim
    • , Sang-Tae Kim
    • , Stephen B. Heitner
    • , David Battaglia
    • , Sacha A. Krieg
    • , David M. Lee
    • , Diana H. Wu
    • , Don P. Wolf
    • , Paula Amato
    • , Sanjiv Kaul
    • , Juan Carlos Izpisua Belmonte
    • , Jin-Soo Kim
    •  & Shoukhrat Mitalipov

    Nature (2018)

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.