A light-sheet microscopy system that is capable of imaging a volume of 100 mm3 with a spatial resolution of 1.6 μm has been built by scientists from China, USA and Japan. The capabilities of their scheme, called subvoxel light-sheet microscopy (SLSM), are demonstrated with the imaging of a variety of samples, including fluorescent beads, 3D cultured cells, a mouse heart and brain, and a zebrafish embryo with cellular resolution. The approach operates by capturing a stack of low-resolution, shift-modulated images that are then processed by a reconstruction algorithm to create a high-resolution output image of the sample. A benefit of the approach is that it removes the need for any mechanical stitching to create a large field of view. Advances in computer power, notably graphics processing units (GPUs), allow the system to operate with acquisition speeds of gigavoxels per minute.
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Graydon, O. Subvoxel light-sheet microscopy. Nature Photon 13, 139 (2019). https://doi.org/10.1038/s41566-019-0384-8
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DOI: https://doi.org/10.1038/s41566-019-0384-8