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SEQUENCING TECHNOLOGY

Epigenetic profiling with ultralow DNA amounts

A microfluidic device powers DNA-methylome sequencing of CpG islands and promoter regions, with minimal sample loss.

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Fig. 1: Comparison of DNA input using MID-RRBS and RRBS workflows for DNA-methylation profiling.
Fig. 2: Pearson correlation coefficients in the CG methylation level between samples processed by MID-RRBS or a Zymo kit.

References

  1. Chen, Z., Li, S., Subramaniam, S., Shyy, J. Y. & Chien, S. Annu. Rev. Biomed. Eng. 19, 195–219 (2017).

    Article  CAS  PubMed  Google Scholar 

  2. Wu, H. & Zhang, Y. Cell 156, 45–68 (2014).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Cokus, S. J. et al. Nature 452, 215–219 (2008).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Gu, H. et al. Nat. Methods 7, 133–136 (2010).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Marabita, F. et al. Epigenetics 8, 333–346 (2013).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Lister, R. et al. Cell 133, 523–536 (2008).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Lister, R. et al. Nature 462, 315–322 (2009).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Meissner, A. et al. Nucleic Acids Res. 33, 5868–5877 (2005).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Gu, H. et al. Nat. Protoc. 6, 468–481 (2011).

    Article  CAS  PubMed  Google Scholar 

  10. Ma, S. et al. Nat. Biomed. Eng. https://doi.org/10.1038/s41551-018-0204-3 (2018).

  11. Malatesta, P. et al. Development 127, 5253–5263 (2000).

    CAS  PubMed  Google Scholar 

  12. Kukushkina, V. et al. Sci. Rep. 7, 3916 (2017).

    Article  PubMed  PubMed Central  Google Scholar 

  13. Varley, K. et al. Genome Res. 23, 555–567 (2013).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Luo, C. et al. Science 357, 600–604 (2017).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

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Correspondence to Zhen Chen.

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Lin, FM., Chien, S. & Chen, Z. Epigenetic profiling with ultralow DNA amounts. Nat Biomed Eng 2, 146–147 (2018). https://doi.org/10.1038/s41551-018-0211-4

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