News & Views | Published:


Cheaper and less variable expansion

Nature Biomedical Engineeringvolume 2pages144145 (2018) | Download Citation

Substituting growth factors with small molecules in the culture medium for the expansion of human pluripotent stem cells reduces costs and lot-to-lot variability.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.


  1. 1.

    Adil, M. M. & Schaffer, D. V. Curr. Opin. Chem. Eng. 15, 24–35 (2017).

  2. 2.

    Yasuda, S.-y. et al. Nat. Biomed. Eng. (2018).

  3. 3.

    Hasegawa, K. et al. Stem Cells Transl. Med. 1, 18–28 (2011).

  4. 4.

    Sokol, S. Y. Development 138, 4341–4350 (2011).

  5. 5.

    Li, X. et al. Cell Stem Cell 8, 46–58 (2011).

  6. 6.

    Horsley, V., Aliprantis, A. O., Polak, L., Glimcher, L. H. & Fuchs, E. Cell 132, 299–310 (2008).

  7. 7.

    Merkle, F. T. et al. Nature 545, 229–233 (2017).

  8. 8.

    Weinberger, L., Ayyash, M., Novershtern, N. & Hanna, J. H. Nat. Rev. Mol. Cell Biol. 17, 155–169 (2016).

Download references

Author information


  1. Department of Chemical and Biomolecular Engineering, University of California Berkeley, Berkeley, CA, USA

    • Maroof M. Adil
    •  & David V. Schaffer
  2. Departments of Bioengineering and Molecular and Cell Biology, and the Helen Wills Neuroscience Institute, University of California Berkeley, Berkeley, CA, USA

    • David V. Schaffer


  1. Search for Maroof M. Adil in:

  2. Search for David V. Schaffer in:

Corresponding author

Correspondence to David V. Schaffer.

About this article

Publication history



Newsletter Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing