Article

Stimulation of 3D osteogenesis by mesenchymal stem cells using a nanovibrational bioreactor

  • Nature Biomedical Engineering 1758770 (2017)
  • doi:10.1038/s41551-017-0127-4
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Abstract

Bone grafts are one of the most commonly transplanted tissues. However, autologous grafts are in short supply, and can be associated with pain and donor-site morbidity. The creation of tissue-engineered bone grafts could help to fulfil clinical demand and provide a crucial resource for drug screening. Here, we show that vibrations of nanoscale amplitude provided by a newly developed bioreactor can differentiate a potential autologous cell source, mesenchymal stem cells (MSCs), into mineralized tissue in 3D. We demonstrate that nanoscale mechanotransduction can stimulate osteogenesis independently of other environmental factors, such as matrix rigidity. We show this by generating mineralized matrix from MSCs seeded in collagen gels with stiffness an order of magnitude below the stiffness of gels needed to induce bone formation in vitro. Our approach is scalable and can be compatible with 3D scaffolds.

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Acknowledgements

This work was supported by grants to S.R. and M.J.D. from the BBSRC, BBSRC/SFI and EPSRC (BB/N012690/1, BB/P00220X/1, EP/N013905/1, EP/N012631/1 and EP/P001114/1), along with a Wolfson Merit Award from The Royal Society and a programme grant from Find a Better Way. M.J.P.B. is funded by SFI grant nos. 11/SIRG/B2135 and 13/RC/2073. P.G.C. was funded by an STFC/BBSRC fellowship. We thank J. Hough, H. Nikukar, I. Tifenbrun and K. Robertson for their discussion, C.-A. Smith for technical support, and E. Manson for help with metabolite analysis. We also thank C. Boyle, S. Robertson and P. Campsie for help with the construction of the bioreactor.

Author information

Author notes

  1. Penelope M. Tsimbouri, Peter G. Childs and Gabriel D. Pemberton contributed equally to this work.

Affiliations

  1. Centre for Cell Engineering, Institute for Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, G12 8QQ, UK

    • Penelope M. Tsimbouri
    • , Gabriel D. Pemberton
    • , Jingli Yang
    • , Vineetha Jayawarna
    • , Wich Orapiriyakul
    • , Adam S. G. Curtis
    •  & Matthew J. Dalby
  2. SUPA, Institute of Thin Films, Sensors and Imaging, University of the West of Scotland, Paisley, PA1 2BE, UK

    • Peter G. Childs
    •  & Stuart Reid
  3. Microenvironments for Medicine, Division of Biomedical Engineering, School of Engineering, College of Science and Engineering, University of Glasgow, Glasgow, G12 8QQ, UK

    • Peter G. Childs
    • , Cristina González-García
    •  & Manuel Salmerón-Sánchez
  4. Glasgow Polyomics facility, College of Medical, Veterinary and Life Sciences, University of Glasgow, Wolfson Wohl Cancer Research Centre, Garscube Campus, Bearsden, G61 1QH, UK

    • Karl Burgess
    •  & Gavin Blackburn
  5. Centre for Research in Medical Devices (CÚRAM), National University of Ireland Galway, Galway, Ireland

    • Dilip Thomas
    • , Catalina Vallejo-Giraldo
    •  & Manus J. P Biggs
  6. SUPA, Department of Biomedical Engineering, Wolfson Centre, Strathclyde University, Glasgow, G4 0NW, UK

    • Stuart Reid

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Contributions

A.S.G.C. was the inspiration behind this work, to whom it is dedicated. P.M.T., P.G.C., G.D.P., J.Y., V.J., W.O., C.G.-G., D.T. and C.V.G. performed the laboratory experiments. P.M.T., P.G.C., G.D.P., W.O., K.B., G.B., M.J.P.B., M.S.-S., S.R. and M.J.D. analysed the data. P.M.T., P.G.C., G.D.P., M.J.P.B., A.S.G.C., M.S.S., S.R. and M.J.D. devised experiments. S.R. and M.J.D. supervised the research. P.M.T., P.G.C., G.D.P., M.S.S., S.R. and M.J.D. wrote the manuscript. P.M.T., P.G.C., S.R. and M.J.D. revised the manuscript.

Competing interests

The authors declare no competing financial interests.

Corresponding authors

Correspondence to Stuart Reid or Matthew J. Dalby.

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