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Generation of self-compatible diploid potato by knockout of S-RNase

Nature Plants volume 4, pages 651–654 (2018)Cite this article

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Abstract

Re-domestication of potato into an inbred line-based diploid crop propagated by seed represents a promising alternative to traditional clonal propagation of tetraploid potato, but self-incompatibility has hindered the development of inbred lines. To address this problem, we created self-compatible diploid potatoes by knocking out the self-incompatibility gene S-RNase using the CRISPR–Cas9 system. This strategy opens new avenues for diploid potato breeding and will also be useful for studying other self-incompatible crops.

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Fig. 1: Knockout of S-RNase overcomes self-incompatibility in potato.

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Acknowledgements

This work was supported by Advanced Technology Talents in Yunnan Province (2013HA025) and the National Natural Science Foundation of China (31601360 to C.Z.). This work was also supported by the Chinese Academy of Agricultural Science (ASTIP-CAAS) and the Shenzhen municipal and Dapeng district governments.

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Author notes

  1. These authors contributed equally to this work: Mingwang Ye, Zhen Peng.

Authors and Affiliations

  1. The CAAS-YNNU Joint Academy of Potato Sciences, Yunnan Normal University, Kunming, China

    Mingwang Ye, Zhen Peng & Yunmei Xu

  2. Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China

    Mingwang Ye, Zhen Peng, Die Tang, Chunzhi Zhang & Sanwen Huang

  3. College of Horticulture, Northwest Agriculture and Forest University, Yangling, China

    Zhongmin Yang & Dawei Li

  4. Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture, Sino-Dutch Joint Laboratory of Horticultural Genomics, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China

    Chunzhi Zhang & Sanwen Huang

Authors
  1. Mingwang Ye
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  2. Zhen Peng
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  3. Die Tang
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  4. Zhongmin Yang
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  5. Dawei Li
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  6. Yunmei Xu
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  7. Chunzhi Zhang
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  8. Sanwen Huang
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Contributions

S.H. and C.Z. conceived and designed the experiments. M.Y. and Z.Y. performed the potato transformation. Z.P. and Y.X. conducted the genotyping and phenotyping of T0 and T1 plants. D.T. performed the bioinformatics analyses. D.L. made the CRISPR–Cas9 construct. C.Z., Z.P., M.Y. and S.H. wrote the manuscript.

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Correspondence to Chunzhi Zhang or Sanwen Huang.

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Supplementary Figures 1–2 and Supplementary Tables 1–3.

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Ye, M., Peng, Z., Tang, D. et al. Generation of self-compatible diploid potato by knockout of S-RNase. Nature Plants 4, 651–654 (2018). https://doi.org/10.1038/s41477-018-0218-6

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