Abstract
The outermost cell layer of plant roots (epidermis) constantly encounters environmental challenges. The epidermal outer plasma membrane domain harbours the PENETRATION3 (PEN3)/ABCG36/PDR8 ATP-binding cassette transporter that confers non-host resistance to several pathogens. Here, we show that the Arabidopsis ENDOPLASMIC RETICULUM-ARRESTED PEN3 (EAP3) BTB/POZ-domain protein specifically mediates PEN3 exit from the endoplasmic reticulum and confers resistance to a root-penetrating fungus, providing prime evidence for BTB/POZ-domain protein-dependent membrane trafficking underlying disease resistance.
This is a preview of subscription content, access via your institution
Access options
Access Nature and 54 other Nature Portfolio journals
Get Nature+, our best-value online-access subscription
$29.99 / 30 days
cancel any time
Subscribe to this journal
Receive 12 digital issues and online access to articles
$119.00 per year
only $9.92 per issue
Rent or buy this article
Prices vary by article type
from$1.95
to$39.95
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Stein, M. et al. Plant Cell 18, 731–746 (2006).
Loehrer, M., Langenbach, C., Goellner, K., Conrath, U. & Schaffrath, U. Mol. Plant Microbe Interact. 21, 1421–1430 (2008).
Underwood, W. & Somerville, S. C. Proc. Natl Acad. Sci. USA 110, 12492–12497 (2013).
Xin, X. F., Nomura, K., Underwood, W. & He, S. Y. Mol. Plant Microbe Interact. 26, 861–867 (2013).
Strader, L. C. & Bartel, B. Plant Cell 21, 1992–2007 (2009).
Łangowski, Ł., Růžička, K., Naramoto, S., Kleine-Vehn, J. & Friml, J. Curr. Biol. 20, 904–908 (2010).
Takano, J. et al. Proc. Natl Acad. Sci. USA 107, 5220–5225 (2010).
Uehara, M. et al. Plant Cell Physiol. 55, 704–714 (2014).
Mao, H., Nakamura, M., Viotti, C. & Grebe, M. Plant Physiol. 172, 2245–2260 (2016).
Underwood, W., Ryan, A. & Somerville, S. C. Mol. Plant 10, 805–820 (2017).
Fendrych, M. et al. Mol. Biol. Cell 24, 510–520 (2013).
Czymmek, K. J. et al. Fungal Genet. Biol. 44, 1011–1023 (2007).
Genschik, P., Sumara, I. & Lechner, E. EMBO J. 32, 2307–2320 (2013).
Dieterle, M. et al. Plant J. 41, 386–399 (2005).
Weber, H. et al. Plant Physiol. 137, 83–93 (2005).
Gingerich, D. J. et al. J. Biol. Chem. 280, 18810–18821 (2005).
Jin, L. et al. Nature 482, 495–500 (2012).
Gorur, A. et al. J. Cell Biol. 216, 1745–1759 (2017).
Dharmasiri, S. et al. Science 312, 1218–1220 (2006).
González, E., Solano, R., Rubio, V., Leyva, A. & Paz-Ares, J. Plant Cell 17, 3500–3512 (2005).
Acknowledgements
We are grateful to D.W. Ehrhardt, J. Friml, N. Geldner, B. Scheres and S.C. Somerville for making available published materials used in this study, as well as to M. Lenhard for critical reading of the manuscript. We thank the Nottingham Arabidopsis Stock Centre for providing seed stocks. This work was supported by ERC consolidator grant ERC-STG-2010 No. 260699 Green-Lat-Pol to M.Gr. and by SNF grant 31003A_165877 to M.Ge.
Author information
Authors and Affiliations
Contributions
M.Gr. conceived the project. M.Gr., H.M., B.A. and M.Ge. designed experiments. H.M. performed all experiments except for Fusarium oxysporum work and for SHORE mapping. T.L. and J.H. performed SHORE mapping of eap3-1. B.A. and M.Ge. performed Fusarium oxysporum experiments. M.Gr. and H.M. wrote the manuscript. All authors read and edited the manuscript prior to publication.
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing financial interests.
Additional information
Publisher’s note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Electronic supplementary material
Supplementary Information
Supplementary Methods, Supplementary References, Supplementary Tables 1 and 2, Supplementary Figures 1–6, Supplementary Methods
Rights and permissions
About this article
Cite this article
Mao, H., Aryal, B., Langenecker, T. et al. Arabidopsis BTB/POZ protein-dependent PENETRATION3 trafficking and disease susceptibility. Nature Plants 3, 854–858 (2017). https://doi.org/10.1038/s41477-017-0039-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/s41477-017-0039-z