Abstract
The CRISPR-Cas9 system has raised hopes for developing personalized gene therapies for complex diseases. Its application for genetic and epigenetic therapies in humans raises concerns over immunogenicity of the bacterially derived Cas9 protein. Here we detect antibodies to Streptococcus pyogenes Cas9 (SpCas9) in at least 5% of 143 healthy individuals. We also report pre-existing human CD8+T cell immunity in the majority of healthy individuals screened. We identify two immunodominant SpCas9 T cell epitopes for HLA-A*02:01 using an enhanced prediction algorithm that incorporates T cell receptor contact residue hydrophobicity and HLA binding and evaluated them by T cell assays using healthy donor PBMCs. In a proof-of-principle study, we demonstrate that Cas9 protein can be modified to eliminate immunodominant epitopes through targeted mutation while preserving its function and specificity. Our study highlights the problem of pre-existing immunity against CRISPR-associated nucleases and offers a potential solution to mitigate the T cell immune response.
Similar content being viewed by others
Introduction
The Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-Cas9 technology has raised hopes for developing personalized gene therapies for complex diseases such as cancer as well as genetic disorders, and is currently entering clinical trials1,2. The history of gene therapy has included both impressive success stories and serious immunologic adverse events3,4,5,6,7,8. The expression of Streptococcus pyogenes Cas9 protein (SpCas9) in mice has evoked both cellular and humoral immune responses9,10, which raises concerns regarding its safety and efficacy as a gene or epi-gene therapy in humans. These pre-clinical models and host immune reactions to other exogenous gene delivery systems11,12,13 suggest that the pathogenic, non-self origin of Cas9 may be immunogenic in humans.
Both B cell and T cell host responses specific to either the transgene or the viral components of adenoviral14,15 and adeno-associated viral (AAV)11,12 vectors have been detected, despite relatively low immunogenicity of AAV vectors. In the case of AAV, specific neutralizing antibodies (Abs) and T cells are frequently detected in healthy donors16,17,18,19 and specific CD8+T cells have been shown to expand following gene delivery18. There has been recent progress in developing strategies to overcome this problem, such as capsid engineering and transient immunosuppression20,21,22. The potential consequences of immune responses to expressed proteins from viral vectors or transgenes include neutralization of the gene product; destruction of the cells expressing it, leading to loss of therapeutic activity or tissue destruction; induction of immune memory that prevents re-administration; and fulminant innate inflammatory responses23,24. More potent immune responses to gene therapies have been observed in humans and non-human primate models compared to mice8,25.
Of the Cas9 orthologs derived from bacterial species, the SpCas9 is the best characterized. S. pyogenes is a ubiquitous pathogen, with an annual incidence of 700 million worldwide26, but the field is only now beginning to explore potential immunity to SpCas9 in humans27,28. CRISPR application for human therapies will span its use both for gene editing (through DNA double-strand breaks) or epigenetic therapies (without DNA double-strand breaks). In fact, recent reports shed light on CRISPR’s ability to activate or repress gene expression in mice29,30,31, which opens the door to a variety of new therapeutic applications such as activating silent genes, compensating for disrupted genes, cell fate reprogramming, or silencing disrupted genes, without the concern over permanent change in DNA sequence. However, unlike the use of Cas9 for gene editing, which may only require Cas9 presence in cells for a few hours, current techniques for CRISPR-based epigenetic therapies require longer term expression of Cas9 in vivo, possibly for weeks and months30,31, which poses the challenge of combating pre-existing immune response towards Cas9. This challenge will need to be addressed before CRISPR application for human therapies, especially for epigenetic therapies, can be fully implemented. Delivery of CRISPR in vivo by incorporating its expression cassette in adeno-associated virus (AAV), will most likely shape many of the initial clinical trials as AAV-based gene delivery is one of the safest and most prevalent forms of gene therapies in human. AAV will enable longer term expression of Cas9, desirable for epigenetic therapies. Therefore, it is highly likely that CRISPR delivery through AAV and its expression within target cells will engage CD8+T cell immunity.
Here, we seek to characterize the pre-existing immune response to SpCas9 in healthy individuals and to identify the immunodominant T cell epitopes with the aim of developing SpCas9 proteins that have diminished capacity to invoke human adaptive response. We identify two immunodominant SpCas9 T cell epitopes for HLA-A*02:01 by an improved prediction algorithm and T cell assays using healthy donor PBMCs. We demonstrate that Cas9 protein can be modified to eliminate immunodominant epitopes through targeted mutation while preserving its function and specificity.
Results
Cas9-specific serum antibodies in healthy controls
We first determined whether healthy individuals have detectable IgG Abs to SpCas9. Of 143 healthy control sera screened, 82 (57.3%) had detectable Abs against S. pyogenes lysate using ELISA (Fig. 1a). Sera with the highest reactivity to S. pyogenes lysate (n = 80) were screened for Abs against recombinant SpCas9, of which 7 (8.8%) were positive (p < 0.0001, two-tailed t-test). At least 5.0% of healthy individuals screened in this study had Cas9-specific Abs (Fig. 1a).
Cas9 candidate T cell epitope prediction
Whether Cas9-specific antibodies impact the efficacy or safety of CRISPR application in human remains to be seen. However, cellular immunity is expected to have a more significant impact in case of CRISPR delivery through viruses. The Cas9 expression cassette, delivered by a viral vector, leads to intracellular expression of this protein in target cells, which could evoke a cellular immune response. We thus focused on investigating the T cell immune response against SpCas9. We predicted HLA-A*02:01-restricted T cell epitopes derived from SpCas9 using a model that uses both MHC binding affinity and biochemical properties of immunogenicity32 (Supplementary Table 1; the top 5 are shown in Fig. 1b). This model incorporates T cell receptor contact residue hydrophobicity and HLA binding prediction, which enhances the efficiency of epitope identification, as we previously reported32. We plotted the calculated normalized binding (Sb) and immunogenicity (Si) scores for each peptide (Fig. 1c) to predict the more immunogenic epitopes, which are expected to have both high HLA binding (low Sb) and more hydrophobicity (high Si). We chose HLA-A*02:01 because it is the most common HLA type in European/North American Caucasians.
T cell epitope mapping of Cas9
We then investigated whether peripheral blood mononuclear cells (PBMCs) derived from healthy individuals had measurable T cell reactivity against the predicted SpCas9 MHC class I epitopes. We synthesized 38 peptides (Supplementary Table 1) and grouped them into 10 pools of 3–4 peptides each. We measured peptide-specific T cell immunity using IFN-γ secretion ELISpot assays with PBMCs derived from 12 healthy individuals (HLA-A*02:01, n = 10; non-HLA-A*02:01, n = 2) and identified immunoreactive epitopes within pools 3 or 5 in 83.0% of the donors tested (90% of the HLA-A*02:01 donors; Fig. 1d). The seven individual peptides from pools 3 and 5 were evaluated by IFN-γ ELISpot and the dominant immunogenic epitopes were SpCas9_240–248 and SpCas9_615-623, designated peptides α and β, from pools 5 and 3, respectively. The subdominant epitopes were found to be γ and δ from pools 3 and 5, respectively. Both peptides α and β are located in the REC lobe of the Cas9 protein (Fig. 1e) that binds the sgRNA and the target DNA heteroduplex33. The individual peptides within pools that were positive for any donor were evaluated for this donor by IFN-γ ELISpot. The immunoreactivity and position of the 38 predicted peptides (a few of which are overlapping) within the Cas9 protein are shown in Fig. 1e.
Peptides α and β are shown as red dots on the epitope prediction plot (Fig. 1c) and their sequences and predicted ranking are shown in Fig. 1b and Supplementary Table 1. As predicted, these peptides had low Sb and high Si values. Both the immunodominant (α and β) and subdominant (γ and δ) T cell epitopes identified by IFN-γ ELISpot were within the top 5 most immunogenic epitopes predicted by our immunogenicity model32. Their ranking as predicted by the consensus method hosted on the IEDB server using default settings was 14, 5, 18, and 4, respectively. Sequence similarity of peptides α and β to amino acid sequences in known proteins was investigated using Protein BLAST and the IEDB epitope database34. This was done to investigate whether there is any chance that the T cell immune response that we are detecting in healthy individuals could be due to previous exposure to another protein of similar sequence. A peptide was considered ‘similar’ to α or β if no more than 2 of 9 amino acid residues (that are not the second or ninth) were not matching (78% similarity). None of these two peptides resembled known epitopes in the IEDB database, but similarity to other Cas9 orthologs and other bacterial proteins was detected (Supplementary Tables 2 and 3). Epitope β has sequence similarity to a peptide derived from the Neisseria meningitidis peptide chain release factor 2 protein (ILEDIVLTL versus ILEGIVLTL). Antigen-specific T cells were expanded for 18 days in vitro by coculturing healthy donor PBMCs with peptide β-pulsed autologous antigen presenting cells (APCs). Cas9-specific CD8+T cell responses were assessed by flow cytometry. CD8+T cells specific for the HLA-A*0201/β pentamer were detected after stimulation (3.09%; Fig. 2a).
Mutated Cas9 proteins have lower immune recognition
We next hypothesized that mutation of the MHC-binding anchor residues of the identified immunogenic epitopes would abolish specific T cell recognition (Fig. 2a). The epitope anchor residues (2nd and 9th) are not only necessary for peptide binding to the MHC groove, but are also crucial for recognition by the T cell receptor32. The percentage of CD8+β pentamer+T cells decreased to 0.3% when APCs were pulsed with the mutated peptide (β2; Fig. 2b) compared with 3.09% with the wild type peptide (β; Fig. 2a). We then examined the reactivity of healthy donor T cells to modified peptides α or β with mutations in residues 2, 9, or both (sequences are shown in Fig. 2c) using IFN-γ ELISpot assay. The epitope-specific T cell reactivity was markedly reduced with the mutant peptides (Supplementary Fig. 1; representative ELISPOT well images are shown in Fig. 2d). The average reduction for the responsive HLA-A*02:01 donors was 25-fold from α to α29 (n = 7; p < 0.03) and 30-fold from β to β29 (n = 8; p < 0.03; Benjamini-Hochberg; Supplementary Fig. 1; Supplementary Table 4). The predicted binding affinity to MHC class II was also decreased for α2 and β2 epitopes, although the experimental significance of this alteration is unknown.
We then generated modified Cas9 constructs by mutating the second residue of peptide α (L241G; Cas9-α2), peptide β (L616G; Cas9-β2), or both (Cas9-α2β2). To measure the effect of mutating the anchor residue of the immunogenic epitopes on T cell recognition of the Cas9 protein, we transiently transfected healthy donor B cell APCs with mRNA encoding wild type Cas9 (WT-Cas9), Cas9-α2, Cas9-β2, or Cas9-α2β2. The T cell response measured by IFN-γ ELISpot after coculturing of Cas9 transfected APCs with autologous PBMCs was significantly decreased for the modified Cas9 proteins (Fig. 2e, f). Introduction of the β2 mutation was the most effective in reducing T cell immunogenicity (5.5-fold, p < 0.0001, two-tailed t-test). This mutation in the REC1 domain (Figs. 1e and 3a) is not located in any of the two regions that are absolutely essential for DNA cleavage, the repeat-interacting (97–150) and the anti-repeat-interacting (312–409) regions33. These results demonstrate that mutating the anchor amino acid of a highly immunogenic epitope can influence the overall immunogenicity of Cas9. Thus, engineering Cas9 variants with reduced immunogenicity potential can be used in conjunction with other strategies for safer CRISPR therapies and even possibly reduce the dosage of systemic immunosuppression needed for patients.
We therefore tested the function of Cas9-β2 in comparison with WT-Cas9 in the context of DNA cleavage and transcriptional modulation. To examine the nuclease activity of Cas9-β2 and compare with WT-Cas9, we targeted Cas9-β2 or WT-Cas9 to an endogenous locus (EMX-1) and measured percent indel formation (Fig. 3b, c). Our data demonstrate that Cas9-β2 retains nuclease capacity in the locus we studied as well as on a synthetic promoter (Fig. 3c, Supplementary Fig. 2A, B).
Next, we determined whether Cas9-β2 can successfully recognize and bind its target DNA leading to transcriptional modulation. We first tested this in the context of enhanced transgene expression from a synthetic CRISPR responsive promoter in HEK293 cells using 14nt gRNAs and aptamer-mediated recruitment of transcriptional modulators similar to what we had shown before (Supplementary Fig. 2C, D). Having shown successful transgene activation, we then investigated whether this variant retains such capacity within the chromosomal contexts of endogenous genes. We transfected the cells with plasmids encoding Cas9-β2 or WT-Cas9 and 14nt gRNAs against two different endogenous genes (TTN and MIAT). qRT-PCR analysis showed that this variant successfully led to target gene expression (Fig. 3d–f). To further characterize Cas9-β2 specificity, we performed genome-wide RNA sequencing after targeting Cas9-β2 or WT-Cas9 to the MIAT locus for transcriptional activation. The results demonstrated no significant increase in undesired off-target activity by Cas9-β2 as compared to WT-Cas9 (Fig. 3g).
To show the extensibility of our approach, we tested the function of Cas9-α2, that has a mutation located in the REC2 domain (Figs. 1e and 3a). Cas9-α2 also demonstrated DNA cleavage and transcriptional modulation functionality comparable with WT-Cas9 (Supplementary Fig. 3A–E). This is consistent with a previous study which showed that Cas9 with a deleted REC2 domain retains its nuclease activity33. When T cells were stimulated with APCs spiked with peptide α2, the percentage of CD8+CD137+T cells (a marker of T cell activation35) was decreased by 2.3-fold as compared to WT peptide α stimulation (Supplementary Fig. 3F).
Immune responses to non-HLA-A*02:01 Cas9 epitopes
We next predicted T cell epitopes derived from SpCas9 for non-HLA-A*02:01 alleles using the IEDB analysis tool (Supplementary Data 1). We selected and synthesized 5–6 epitopes (sequences shown in Supplementary Table 5 and highlighted in Supplementary Data 1) for each of 7 common alleles (A*01:01, A*03:01, A*11:01, A*24:02, B*08:01, B*44:01, B*55:01) and used them to stimulate PBMCs derived from 6 healthy donors with the corresponding HLA alleles. Of the peptides and alleles screened, we detected peptide-specific T cell immune responses (in more than one donor) against peptide 25 in two HLA-A*24:02 donors (p < 0.05; results for HLA-A*24:02 are shown as an example in Fig. 4a).
Immune responses to MHC Class II Cas9 epitopes
We also predicted MHC class II binding epitopes for the SpCas9 protein to HLA-DRB1 (10 alleles), HLA-DQ (5 alleles), and HLA-DP (8 alleles) using the IEDB analysis tool (Supplementary Data 2). For MHC class II, epitope α is predicted to be a top binder to HLA-DRB1*01:02 and epitope β a top binder to HLA-DPA1*01:03 and DPB1*02:01 (Supplementary Data 2). We selected and synthesized SpCas9 long peptides that include epitopes in the top 2% of predicted MHC class II binders (sequences and alleles shown in Supplementary Table 6 and highlighted in Supplementary Data 2) and measured peptide-specific CD4+ T cell immunity using IFN-γ secretion ELISpot assays with CD8-depleted PBMCs derived from three healthy individuals. We detected limited CD4+ immune responses against the peptides tested (Fig. 4b). This prompted us to evaluate the CD4+ T cell immune response against the whole Cas9 protein compared with a positive control protein. We stimulated healthy donor CD8-depleted PBMCs with recombinant SpCas9 or EBNA proteins for 10 days and detected a modest response (less than twofold of that of unstimulated cells; Fig. 4c). We then sought to investigate whether the immune response that we detected against peptide β was primarily derived by CD4+ or CD8+ T lymphocytes. We stimulated MACS sorted CD4+ or CD8+ T cells isolated from PBMCs from 3 donors with peptide β and detected a primarily CD8+ response in all 3 donors (Fig. 4d).
Discussion
The detection of pre-existing B cell and T cell immunity to the most widely used nuclease ortholog of the CRISPR-Cas9 tool in a significant proportion of healthy humans confirms previous studies in mice9,10 and a recent study in humans27 and sheds light on the need for more studies of the immunological risks of this system. A recent study reported SpCas9-specific T-cell-mediated killing of autologous CRISPR-treated APCs in vitro28. However, the consequences of Cas9-specific immunity in vivo remain to be seen. The CD8+T cell immunity we observed is likely memory responses, as they are observed without ex vivo stimulation. However, following 18 days of T cell stimulation by peptides α or β, expansion of naïve T cells is not precluded. This suggests that even in the absence of a pre-existing immune response, the expression of Cas9 in naïve individuals may trigger a T cell response that could prevent subsequent administration. This could be avoided by switching to Cas9 orthologs from other bacterial species, but can be difficult given the epitope conservation across Cas9 proteins from multiple Streptococcus species and resemblance to sequences from other bacterial proteins such as the common pathogen N. meningitidis, that asymptomatically colonizes the nasopharynx in 10% of the population36. Therefore, selective deimmunization (immunosilencing) of Cas9 can represent an attractive alternative, particularly in patients where high-dose systemic immunosuppression is contraindicated, such as in patients with chronic infectious diseases. This strategy can be important in particular when longer term expression of Cas9 will be desired.
Using IFN-γ ELISpot, we detected a modest CD4+ immune response against recombinant SpCas9 protein and no response to any of the class II SpCas9 peptides that we used. This is consistent with a recent study that found the majority of the CD4+ response against SpCas9 to be restricted to the Treg compartment with minimum IFN-γ and TNF-α secretion28. While a more comprehensive epitope mapping study is needed, we did not experimentally detect immunodominant epitopes among the top binders of the non-HLA-A*02:01 alleles. We show here that silencing one epitope for an HLA-A*02:01 donor (who was also positive for A*11:01, B*39:01, and B*46:01) was sufficient to significantly reduce Cas9 immunogenicity. However, whether other mutations need to be introduced to the protein for complete silencing in HLA-A*02:01 negative individuals needs further investigation. Conventional methods of deimmunizing non-human therapeutic proteins rely on trial-and-error mutagenesis or machine learning and often includes deletion of whole regions of the protein37,38,39,40,41. Here, as a general principle, we show that alteration of one of the anchor residues of an immunodominant epitope abolished specific T cell recognition. However, HLA allotype diversity and the existence of numerous epitopes in the large Cas9 protein may complicate the process of complete deimmunization. The overall impact of removal of select immunodominant epitopes remains to be seen; both reduction42 and enhancement43 of the immunogenicity of subdominant epitopes have been reported with similar approaches for other proteins.
The top binding T cell epitopes within Cas9 that are most promiscuous for common HLA class I and class II alleles have been recently predicted in silico using IEDB44. However, this is the first study that experimentally validates predicted immunodominant epitopes. None of the HLA-A*02:01 epitopes that we report overlap with the peptides previously predicted44. This is not surprising since our prediction model is optimized for HLA-A*02:01. Thus, improved algorithms are needed to predict epitopes that hold up in experimental validation, as we show here. The use of CRISPR-Cas9 in humans may eventually necessitate creating HLA type-specific Cas9 variants, particularly for applications that require long-term Cas9 expression.
Non-specific localized immune suppressive approaches, such as those used by tumor cells and some viruses may complement these strategies for complete deimmunization. Antigen presentation can be blocked by viral proteins interfering with antigen presentation (VIPRs), such as the adenoviral E319K or US2 and US11 from the human cytomegalovirus45 or molecules that inhibit proteasomal antigen processing such as the Epstein-Barr virus Gly-Ala repeat46. Deimmunized Cas9 may be useful in reduction of the dosage of other immunomodulatory measures needed to be co-administered in patients, thus facilitating therapeutic CRISPR applications as we develop better understanding of the immunological consequences of this system.
Methods
Detection of Cas9-specific serum antibodies
Healthy control sera (n = 143) used in this study, and previously47, are a subset of a molecular epidemiology study of head and neck cancer at the MD Anderson Cancer Center, collected between January 2006 and September 2008. Samples were collected using a standardized sample collection protocol and stored at -80 °C until use. All relevant ethical regulations for work with human participants were followed and written informed consent was obtained from all participants under the Arizona State University institutional review board approval. Participants were ethnically diverse. Sample size was determined based on prior experience and similar experiments in the literature. There was no group allocation and the investigators were blinded to participants’ information. S. pyogenes lysate was prepared by sonication of bacterial pellets from overnight cultures of S. pyogenes ATCC 19615 in the presence of 1 pill of cOmplete Protease Inhibitor (Sigma-Aldrich) after 3 cycles of freezing and thawing. Serum antibody detection was performed using ELISA. 96-well plates were coated with 20 μg/mL of recombinant S. pyogenes Cas9 nuclease (Takara Bio USA, Mountain View, CA), recombinant Epstein-Barr virus nuclear antigen 1 (EBNA; Advanced Biotechnologies Inc., Eldersburg, MD), human hemoglobin (Sigma-Aldrich) or S. pyogenes lysate. Sera were diluted 1:50 in 10% E. coli lysate prepared in 5% milk-PBST (0.2% tween)48, incubated with shaking for 2 h at room temperature, and added to the specified wells in duplicate. Horseradish peroxidase (HRP) anti-human IgG Abs (Jackson ImmunoResearch Laboratories, West Grove, PA) were added at 1:10,000, and detected using Supersignal ELISA Femto Chemiluminescent substrate (Thermo Fisher Scientific, Waltham, MA). Luminescence was detected as relative light units (RLU) on a Glomax 96 Microplate Luminometer (Promega, Madison, WI) at 425 nm. The cutoff value was defined as any reactivity higher than the top 99% of RLU values for human hemoglobin (Fig. 1a, dotted line).
Cas9 candidate T cell epitope prediction
We predicted MHC class I restricted 9-mer and 10-mer candidate epitopes derived from the Cas9 protein (Uniprot - Q99ZW2) for HLA A*02:01. The protein reference sequence was entered into 5 different prediction algorithms; 3 MHC-binding: IEDB-consensus binding49, NetMHCpan binding50, Syfpeithi51, and 2 antigen-processing algorithms: IEDB-consensus processing, ANN processing52. The individual scores from each of the prediction algorithms were then normalized within the pool of predicted peptides after exclusion of poor binders, and the average normalized binding scores were used to re-rank the candidate peptides. The top 38 candidate peptides (Supplementary Table 1) were selected for experimental testing. The IEDB consensus MHC-binding prediction algorithm (http://www.iedb.org/) was applied to obtain a list of high binding Cas9 peptides, each of which was assigned a normalized binding score (Sb). The immunogenicity score (Si) was calculated for each peptide based on its amino acid hydrophobicity (ANN-Hydro)32. MHC class II epitopes were predicted using the recommended setting on IEDB. We used the Visual Molecular Dynamics software53 (http://www.ks.uiuc.edu/Research/vmd/) to generate the Cas9 protein structure (PDB ID: 4CMP, Fig. 3a).
Ex vivo stimulation and epitope mapping of Cas9 by ELISpot
All peripheral blood mononuclear cells (PBMCs) were obtained from healthy individuals with written informed consent under the Arizona State University institutional review board and all relevant ethical regulations for work with human participants were followed. Participants were ethnically diverse. Sample size was determined based on prior experience and similar experiments in the literature. There was no group allocation and the investigators were blinded to participants’ information. If PBMCs from a given donor were not reactive to the positive control, the donor was excluded from the study. PBMCs were isolated from fresh heparinized blood by Ficoll–Hypaque (GE Healthcare, UK) density gradient centrifugation and stimulated. Briefly, predicted Cas9 peptides with Sb < 0.148 (n = 38) were synthesized (>80% purity) by Proimmune, UK. Each peptide was reconstituted at 1 mg/mL in sterile PBS and pools were created by mixing 3–4 candidate peptides. Sterile multiscreen ELISpot plates (Merck Millipore, Billerica, MA, USA) were coated overnight with 5 μg/well of anti-IFN-γ capture antibody (clone D1K, # 3420-3-250, Mabtech, USA) diluted in sterile PBS. Frozen PBMCs were thawed rapidly and recombinant human IL-2 (20 U/mL, R&D Systems) was added. They were then stimulated in triplicates with 10 μg/mL Cas9 peptide pools (or individual peptides), pre-mixed CEF pool as a positive control (ProImmune, UK), or DMSO as a negative control in the anti-IFN-γ-coated ELISpot plates, (Merck Millipore, Billerica, MA, USA) and incubated in a 37 °C, 5% CO2 incubator for 48 h. Plates were washed three times for 5 min each with ELISpot buffer (PBS+0.5% FBS) and incubated with 1 μg/mL anti-IFN-γ secondary detection antibody (clone 7-B6-1, # 3420-6-250, Mabtech, USA) for 2 h at room temperature, washed and incubated with 1 μg/mL Streptavidin ALP conjugate for 1 h at room temperature. The wells were washed again with ELISpot buffer and spots were developed by incubating for 8–10 min with detection buffer (33 μL NBT, 16.5 μL BCIP, in 100 mM Tris-HCl pH 9, 1 mM MgCl2, 150 mM NaCl). Plates were left to dry for 2 days and spots were read using the AID ELISpot reader (Autoimmun Diagnostika GmbH, Germany). The average number of spot forming units for each triplicate was calculated for each test peptide or peptide pool and subtracted from the background signal.
Autologous APC generation from healthy individual PBMCs
Autologous CD40L-activated B cell APCs were generated from healthy donors by incubating whole PBMCs with irradiated (32 Gy) K562-cell line expressing human CD40L (KCD40L) at a ratio of 4:1 (800,000 PBMCs to 200,000 irradiated KCD40Ls) in each well. The cells were maintained in B cell media (BCM) consisting of IMDM (Gibco, USA), 10% heat-inactivated human serum (Gemini Bio Products, CA, USA), and Antibiotic-Antimycotic (Anti-Anti, Gibco, USA). BCM was supplemented with 10 ng/mL recombinant human IL-4 (R&D Systems, MN, USA), 2 μg/mL Cyclosporin A (Sigma-Aldrich, CA, USA), and insulin transferrin supplement (ITES, Lonza, MD, USA). APCs were re-stimulated with fresh irradiated KCD40Ls on days 5 and 10, after washing with PBS and expanding into a whole 24-well plate. After two weeks, APC purity was assessed by CD19+ CD86+ expressing cells using flow cytometry, and were used for T cell stimulation after >90% purity. APCs were either restimulated up to 4 weeks or cryopreserved for re-expansion as necessary.
T cell stimulation by autologous APCs
Antigen-specific T cells were detected by stimulating healthy donor B cell APCs by either peptide pulsing of specific Cas9 epitopes, or by transfecting with mRNA encoding the whole WT or modified Cas9 proteins. Peptide pulsing of APCs was done under BCM 5% human serum, with recombinant IL-4. Transfection of APCs was done with primary P3 buffer in a Lonza 4D Nucleofector and program EO117 (Lonza, MD, USA) and incubated in BCM-10% human serum and IL-4. Twenty-four hrs later, on day 1, APCs were washed and incubated with thawed whole PBMCs at a ratio of 1:2 (200,000 APCs: 400,000 PBMCs) in a 24-well plate in BCM supplemented with 20 U/mL recombinant human IL-2 (R&D Systems, MN, USA) and 5 ng/mL IL-7 (R&D Systems, MN, USA). On day 5, partial media exchange was performed by replacing half the well with fresh BCM and IL-2. On day 10, fresh APCs were peptide pulsed in a new 24-well plate. On day 11, expanded T cells were restimulated with peptide-pulsed APCs similar to day 1. T cells were used for T cell assays or immunophenotyped after day 18.
Flow cytometry staining for T cells
Cells were washed once in MACS buffer (containing PBS, 1% BSA, 0.5 mM EDTA), centrifuged at 550 g for 5 min and re-suspended in 200 μL MACS buffer. Cells were stained in 100 μL of staining buffer containing anti-CD137, conjugated with phycoerythrin (PE, clone 4B4-1; BD Biosciences, USA), anti-CD8-PC5 (clone B9.11; Beckman Coulter 1:100), anti-CD4 (clone SK3; BioLegend, 1:200), anti-CD14 (clone 63D3; BioLegend, 1:200), and anti-CD19 (clone HIB19; BioLegend,1:200), all conjugated to Fluorescein isothiocyanate (FITC) for exclusion gates, for 30 min on ice. Samples were covered and incubated for 30 min on ice, washed twice in PBS, and resuspended in 1 mL PBS prior to analysis.
Pentamer staining for T cell immunophenotyping
The following HLA-A*02:01 PE-conjugated Cas9 pentamers were obtained from ProImmune: F2A-D-CUS-A*02:01-ILEDIVLTL-Pentamer and 007-Influenza A MP 58-66-GILGFVFTL-Pentamer. T cells were washed twice in MACS buffer with 5% human serum and centrifuged at 550 g for 5 min each time. They were then re-suspended in 100 μL staining buffer (MACS buffer, with 5% human serum and 1 mM Dasatanib (ThermoFisher Scientific, MA, USA). Each of the pentamers was added to resuspended T cells, stimulated with the respective peptide or APCs at a concentration of 1:100. Samples were incubated at room temperature for 30 min in the dark, then washed twice in MACS buffer. Cells were stained in 100 μL MACS buffer with anti-CD8-PC5, anti-CD4-FITC, anti-CD14-FITC, and anti-CD19-FITC for exclusion gates, Samples were then washed twice with PBS and analyzed by flow cytometry. For flow cytometric analysis, all samples were acquired with Attune flow cytometer (ThermoFisher Scientific, MA, USA) and analyzed using the Attune software. Gates for expression of different markers and pentamers were determined based on flow minus one (FMO) samples for each color after doublet discrimination (Supplementary Fig. 4). Percentages from each of the gated populations were used for the analysis.
Modified Cas9 plasmids: human codon-optimized Streptococcus pyogenes Cas9 sequence was amplified from pSpCas9 (pX330; Addgene plasmid ID: 42230), using forward and reverse primers and inserted within gateway entry vectors using golden gate reaction. Desired mutations were designed within gBlocks (Integrated DNA Technologies). The gBlocks and amplicons were then cloned into gateway entry vectors using golden gate reaction. All the primer sequences are listed in Supplementary Table 7 and all the gBlocks sequences are listed in Supplementary Note 1. Next, the Cas9 vectors and CAG promoter cassettes were cloned into an appropriate gateway destination vector via LR reaction (Invitrogen).
U6-sgRNA-MS2 plasmids: these plasmids were constructed by inserting either 14 bp or 20 bp spacers of gRNAs into sgRNA (MS2) cloning backbone (Addgene plasmid ID: 61424) at BbsI site. All the gRNA sequences are listed in Supplementary Table 8.
Cell culture for endogenous target mutation and activation
HEK293FT cell line was purchased from ATCC and maintained in Dulbecco’s modified Eagle’s medium (DMEM - Life Technologies) containing 10% fetal bovine serum (FBS - Life Technologies), 2 mM glutamine, 1 mM sodium pyruvate (Life Technologies), and 1% penicillin-streptomycin (Life Technologies) in incubators at 37 °C and 5% CO2. Polyethylenimine (PEI) was used to transfect HEK293FT cells seeded into 24-well plates. Transfection complexes were prepared according to manufacturer’s instructions (Polysciences).
Fluorescent reporter assay for quantifying Cas9 function
For the experiment assessing Cas9 cleavage capacity at a synthetic promoter, HEK293FT cells were co-transfected with 200 ng gRNA, 200 ng Cas9 constructs, 50 ng reporter plasmid, and 25 ng enhanced blue florescent protein (EBFP) expressing plasmid as the transfection control. For the experiment assessing Cas9 transcriptional activation capacity at a synthetic promoter, HEK293FT cells were co-transfected with 50 ng gRNA, 70 ng Cas9 constructs, 100 ng MS2-P65-HSF1-GFP (Addgene plasmid ID: 61423), 200 ng reporter plasmid. Fluorescent reporter experiments were performed 48 h after transfection. Nonviable cells were excluded from the analysis using 7-AAD (7-amino-actinomycin D) conjugated with PerCP. Next, we selected cells expressing EBFP >2 × 102 A.U. or GFP >2 × 102 A.U. (transfection markers) in the cleavage and activation experiments, respectively to exclude untransfected cells (Supplementary Fig. 4). Flow cytometry was performed using a FACSCelesta flow cytometer (Becton Dickson) with HTS. Flow cytometry data were analyzed using FlowJo software. Untransfected controls were included in each experiment. Experiments underwent initial validation in duplicate and then were repeated in triplicate for the final manuscript.
Quantitative RT-PCR analysis
HEK293FT cells were co-transfected with 10 ng gRNA, 200 ng Cas9 constructs, 100 ng MS2-P65-HSF1 (Addgene plasmid ID: 61423) and 25 ng EBFP plasmid as the transfection control. Cells were lysed, and RNA was extracted using RNeasy Plus mini kit (Qiagen) 72 h post transfection, followed by cDNA synthesis using the High-Capacity RNA-to-cDNA Kit (Thermo Fisher). qRT-PCR was performed using SYBR Green PCR Master Mix (Thermo Fisher). All analyses were normalized to 18S rRNA (ΔCt) and fold changes were calculated against untransfected controls (2−ΔΔCt). Primer sequences for qPCR are listed in Supplementary Table 7.
Endogenous indel analysis
HEK293FT cells were co-transfected with 200 ng of Cas9 plasmids, 10 ng of gRNA coding cassette, and 25 ng EBFP plasmid as the transfection control. 72 h later, transfected cells were dissociated and spun down at 200 g for 5 min at room temperature. Genomic DNA was extracted using 50 µl of QuickExtract DNA extraction solution (Epicentre) according to the manufacturer’s instructions. Genomic DNA was amplified by PCR using primers flanking the targeted region. Illumina Tru-Seq library was created by ligating partial adapters and a unique barcode to the DNA samples. Next, a small number of PCR cycles was performed to complete the partial adapters. Equal amounts of each sample were then pooled and sequenced on Illumina Tru-Seq platform with 2 × 150 run parameters, which yielded approximately 80,000 reads per sample. Sequencing was performed using a 2 × 150 paired-end (PE) configuration by CCIB DNA Core Facility at Massachusetts General Hospital (Cambridge, MA, USA). The reads were aligned to the target gene reference in Mus musculus genome using Geneious software, 9-1-5. To detect the indels (insertions and deletions of nucleic acid sequence at the site of double-strand break), each mutation was evaluated carefully in order to exclude the ones that are caused by sequencing error or any off-target mutation. The variant frequencies (percentage to total) assigned to each read containing indels were summed up. i.e. indel percentage = total number of indel containing reads/ total number of reads. The minimum number of analyzed reads per sample was 70,000.
RNA sequencing for quantifying activator specificity
HEK293FT cells were co-transfected with 10 ng gRNA targeting MIAT locus, 200 ng Cas9 constructs, 100 ng MS2-P65-HSF1 (Addgene plasmid ID: 61423), and 25 ng transfection control. Total RNA was extracted 72 h post transfection using RNeasy Plus mini kit (Qiagen) and sent to UCLA TCGB core on dry ice. Ribosomal RNA depletion, and single read library preparation were performed at UCLA core followed by RNA sequencing using NextSeq500. Coverage was 14 million reads per sample. FASTQ files with single-ended 75 bp reads were then aligned to the human GRCh38 reference genome sequence (Ensembl release 90) with STAR54, and uniquely-mapped read counts (an average of 14.8 million reads per sample) were obtained with Cufflink55. The read counts for each sample were then normalized for the library size to CPM (counts per million reads) with edgeR56. Custom R scripts were then used to generate plots.
Reporting summary
Further information on experimental design is available in the Nature Research Reporting Summary linked to this article.
Data availability
The datasets generated and/or analyzed during the current study have been submitted to the Gene Expression Omnibus (GEO) repository and are accessible through GEO series accession number GSE125522 . Source data for this manuscript are available in the Source Data file. All other relevant data are available from the authors upon reasonable request.
References
Cyranoski, D. Chinese scientists to pioneer first human CRISPR trial. Nature 535, 476 (2016).
Reardon, S. First CRISPR clinical trial gets green light from US panel. Nature 531, 160–163 (2016).
Cavazzana-Calvo, M. et al. Gene therapy of human severe combined immunodeficiency (SCID)-X1 disease. Science 288, 669–672 (2000).
Gaspar, H. B. et al. Gene therapy of X-linked severe combined immunodeficiency by use of a pseudotyped gammaretroviral vector. Lancet 364, 2181–2187 (2004).
Howe, S. J. et al. Insertional mutagenesis combined with acquired somatic mutations causes leukemogenesis following gene therapy of SCID-X1 patients. J. Clin. Invest. 118, 3143–3150 (2008).
Marshall, E. Gene therapy death prompts review of adenovirus vector. Science 286, 2244–2245 (1999).
Hacein-Bey-Abina, S. et al. Sustained correction of X-linked severe combined immunodeficiency by ex vivo gene therapy. N. Engl. J. Med. 346, 1185–1193 (2002).
Manno, C. S. et al. Successful transduction of liver in hemophilia by AAV-Factor IX and limitations imposed by the host immune response. Nat. Med. 12, 342–347 (2006).
Chew, W. L. et al. A multifunctional AAV-CRISPR-Cas9 and its host response. Nat. Methods 13, 868–874 (2016).
Wang, D. et al. Adenovirus-mediated somatic genome editing of Pten by CRISPR/Cas9 in mouse liver in spite of Cas9-specific immune responses. Hum. Gene Ther. 26, 432–442 (2015).
Mays, L. E. & Wilson, J. M. The complex and evolving story of T cell activation to AAV vector-encoded transgene products. Mol. Ther. 19, 16–27 (2011).
Mingozzi, F. & High, K. A. Immune responses to AAV vectors: overcoming barriers to successful gene therapy. Blood 122, 23–36 (2013).
Yin, H. et al. Non-viral vectors for gene-based therapy. Nat. Rev. Genet. 15, 541–555 (2014).
Ahi, Y. S., Bangari, D. S. & Mittal, S. K. Adenoviral vector immunity: its Implications and circumvention strategies. Curr. Gene. Ther. 11, 307–320 (2011).
Aldhamen, Y. A. & Amalfitano, A. In Adenoviral Vectors for Gene Therapy 2nd edn, 391–422 (Academic Press, Cambridge, Massachusetts 2016).
Thwaite, R., Pages, G., Chillon, M. & Bosch, A. AAVrh.10 immunogenicity in mice and humans. Relevance of antibody cross-reactivity in human gene therapy. Gene Ther. 22, 196–201 (2015).
Boutin, S. et al. Prevalence of serum IgG and neutralizing factors against adeno-associated virus (AAV) types 1, 2, 5, 6, 8, and 9 in the healthy population: implications for gene therapy using AAV vectors. Hum. Gene Ther. 21, 704–712 (2010).
Mingozzi, F. et al. CD8(+) T-cell responses to adeno-associated virus capsid in humans. Nat. Med. 13, 419–422 (2007).
Scallan, C. D. et al. Human immunoglobulin inhibits liver transduction by AAV vectors at low AAV2 neutralizing titers in SCID mice. Blood 107, 1810–1817 (2006).
Bartel, M., Schaffer, D. & Buning, H. Enhancing the clinical potential of AAV vectors by capsid engineering to evade pre-existing immunity. Front. Microbiol. 2, 204 (2011).
Martino, A. T. et al. Engineered AAV vector minimizes in vivo targeting of transduced hepatocytes by capsid-specific CD8+T cells. Blood 121, 2224–2233 (2013).
Mingozzi, F. et al. Overcoming preexisting humoral immunity to AAV using capsid decoys. Sci. Transl. Med. 5, 194ra192 (2013).
Jiang, H. et al. Effects of transient immunosuppression on adenoassociated, virus-mediated, liver-directed gene transfer in rhesus macaques and implications for human gene therapy. Blood 108, 3321–3328 (2006).
Kay, M. A. State-of-the-art gene-based therapies: the road ahead. Nat. Rev. Genet. 12, 316–328 (2011).
Gao, G. et al. Adeno-associated virus-mediated gene transfer to nonhuman primate liver can elicit destructive transgene-specific T cell responses. Hum. Gene Ther. 20, 930–942 (2009).
Carapetis, J. R., Steer, A. C., Mulholland, E. K. & Weber, M. The global burden of group A streptococcal diseases. Lancet Infect. Dis. 5, 685–694 (2005).
Simhadri, V. L. et al. Prevalence of pre-existing antibodies to CRISPR-associated nuclease Cas9 in the USA population. Mol. Ther. Methods Clin. Dev. 10, 105–112 (2018).
Wagner, D. L. et al. High prevalence of Streptococcus pyogenes Cas9-reactive T cells within the adult human population. Nat. Med. 25, 242–248 (2018).
Ibrahim, S. H. & Robertson, K. D. Use of the CRISPR/Cas9-based epigenetic gene activation system in vivo: a new potential therapeutic modality. Hepatology 68, 1191–1193 (2018).
Liao, H. K. et al. In vivo target gene activation via CRISPR/Cas9-mediated trans-epigenetic modulation. Cell 171, 1495–1507 e1415 (2017).
Zheng, Y. et al. CRISPR interference-based specific and efficient gene inactivation in the brain. Nat. Neurosci. 21, 447–454 (2018).
Chowell, D. et al. TCR contact residue hydrophobicity is a hallmark of immunogenic CD8+T cell epitopes. Proc. Natl Acad. Sci. USA 112, E1754–E1762 (2015).
Nishimasu, H. et al. Crystal structure of cas9 in complex with guide RNA and target DNA. Cell 156, 935–949 (2014).
Vita, R. et al. The immune epitope database (IEDB) 3.0. Nucleic Acids Res. 43, D405–D412 (2015).
Wolfl, M. et al. Activation-induced expression of CD137 permits detection, isolation, and expansion of the full repertoire of CD8+T cells responding to antigen without requiring knowledge of epitope specificities. Blood 110, 201–210 (2007).
Pollard, A. J. & Maiden, M. C. J. Meningococcal Vaccines (Humana Press, Totowa, New Jersey 2001).
King, C. et al. Removing T-cell epitopes with computational protein design. Proc. Natl Acad. Sci. USA 111, 8577–8582 (2014).
Mazor, R. et al. Rational design of low immunogenic anti CD25 recombinant immunotoxin for T cell malignancies by elimination of T cell epitopes in PE38. Cell. Immunol. 313, 59–66 (2017).
Salvat, R. S. et al. Computationally optimized deimmunization libraries yield highly mutated enzymes with low immunogenicity and enhanced activity. Proc. Natl Acad. Sci. USA 114, E5085–E5093 (2017).
Cantor, J. R. et al. Therapeutic enzyme deimmunization by combinatorial T-cell epitope removal using neutral drift. Proc. Natl Acad. Sci. USA 108, 1272–1277 (2011).
Tangri, S. et al. Rationally engineered therapeutic proteins with reduced immunogenicity. J. Immunol. 174, 3187–3196 (2005).
Yeung, V. P. et al. Elimination of an immunodominant CD4+T cell epitope in human IFN-β does not result in an in vivo response directed at the subdominant epitope. J. Immunol. 172, 6658–6665 (2004).
Mok, H., Lee, S., Wright, D. W. & Crowe, J. E. Enhancement of the CD8+T cell response to a subdominant epitope of respiratory syncytial virus by deletion of an immunodominant epitope. Vaccine 26, 4775–4782 (2008).
Chew, W. L. Immunity to CRISPR Cas9 and Cas12a therapeutics. Wiley Interdiscip. Rev. Syst. Biol. Med. 10, (2018).
Yewdell, J. W. & Hill, A. B. Viral interference with antigen presentation. Nat. Immunol. 3, 1019 (2002).
Levitskaya, J. et al. Inhibition of antigen processing by the internal repeat region of the Epstein-Barr virus nuclear antigen-1. Nature 375, 685–688 (1995).
Anderson, K. S. et al. HPV16 antibodies as risk factors for oropharyngeal cancer and their association with tumor HPV and smoking status. Oral. Oncol. 51, 662–667 (2015).
Wang, J. et al. A versatile protein microarray platform enabling antibody profiling against denatured proteins. Proteomics Clin. Appl. 7, 378–383 (2013).
Moutaftsi, M. et al. A consensus epitope prediction approach identifies the breadth of murine TCD8+-cell responses to vaccinia virus. Nat. Biotechnol. 24, 817 (2006).
Hoof, I. et al. NetMHCpan, a method for MHC class I binding prediction beyond humans. Immunogenetics 61, 1 (2009).
Rammensee, H.-G., Bachmann, J., Emmerich, N. P. N., Bachor, O. A. & Stevanović, S. SYFPEITHI: database for MHC ligands and peptide motifs. Immunogenetics 50, 213–219 (1999).
Tenzer, S. et al. Modeling the MHC class I pathway by combining predictions of proteasomal cleavage, TAP transport and MHC class I binding. Cell. Mol. Life Sci. 62, 1025–1037 (2005).
Humphrey, W., Dalke, A. & Schulten, K. VMD: visual molecular dynamics. J. Mol. Graph. 14, 33–38 (1996).
Dobin, A. et al. STAR: ultrafast universal RNA-seq aligner. Bioinformatics 29, 15–21 (2013).
Trapnell, C. et al. Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks. Nat. Protoc. 7, 562–578 (2012).
Robinson, M. D., McCarthy, D. J. & Smyth, G. K. edgeR: a Bioconductor package for differential expression analysis of digital gene expression data. Bioinformatics 26, 139–140 (2010).
Acknowledgements
We thank Dr. Erich Sturgis for providing the serum samples, Dr. Diego Chowell for support with the computational prediction model, and Padhmavathy Yuvaraj for technical assistance. We thank all the other members of the Anderson, Kiani, and Ebrahimkhani labs for their assistance and insightful discussions. This work was supported by ASU institutional funds, the startup fund by the School of Biological and Health Systems Engineering of ASU, National Institute of Biomedical Imaging and Bioengineering R01 grant (1R01EB024562-01A1), and DARPA Young Faculty Award (D16AP00047) to S.K. We thank the Center for Computational and Integrative Biology (CCIB) DNA core facility at Massachusetts General Hospital and Genomics and Bioinformatics (TCGB) core at UCLA for the DNA and RNA sequencing services.
Author information
Authors and Affiliations
Contributions
S.R.F. and R.E. designed experiments, performed experiments, and analyzed data. F.M. generated Cas9 variant constructs, performed Cas9 functional analysis experiments, and analyzed data. S. Krishna generated predicted Cas9 epitopes and designed and assisted with the T cell experiments. F.M. and S. Krishna contributed equally. J.G.P. analyzed RNA seq data. M.R.E. helped with the design of experiments and interpretation of data. S.K. and K.S.A. supervised this study. R.E. took the lead in writing the manuscript with input from S.R.F., S.K. and K.S.A. and support of all the other authors.
Corresponding authors
Ethics declarations
Competing interests
The Arizona Board of Regents on behalf of Arizona State University has submitted a patent application (application number: PCT/US18/29937, filed 27 April 2018), with authors S.R.F., R.E., F.M., S.K., M.R.E., S.K. and K.S.A. The patent application was published on 24 Jan 2019 and it is awaiting national stage entry. It includes a methods and compositions for reducing an undesirable T cell immune response in human patients before or during gene therapy using the CRISPR/Cas9-based genetic modulation. Also, it includes DNA sequences of cas9-α2, -β2, -α2β2, and sequences of guide RNAs and primers. The remaining authors declare no competing interests.
Additional information
Journal peer review information: Nature Communications thanks Thorsten Buch and the other anonymous reviewer(s) for their contribution to the peer review of this work. Peer reviewer reports are available.
Publisher’s note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Source data
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Ferdosi, S.R., Ewaisha, R., Moghadam, F. et al. Multifunctional CRISPR-Cas9 with engineered immunosilenced human T cell epitopes. Nat Commun 10, 1842 (2019). https://doi.org/10.1038/s41467-019-09693-x
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/s41467-019-09693-x
This article is cited by
-
Engineering Cas9: next generation of genomic editors
Applied Microbiology and Biotechnology (2024)
-
Bioinformatic and literature assessment of toxicity and allergenicity of a CRISPR-Cas9 engineered gene drive to control Anopheles gambiae the mosquito vector of human malaria
Malaria Journal (2023)
-
Viral vectors and extracellular vesicles: innate delivery systems utilized in CRISPR/Cas-mediated cancer therapy
Cancer Gene Therapy (2023)
-
CRISPR-Cas9 system: a novel and promising era of genotherapy for beta-hemoglobinopathies, hematological malignancy, and hemophilia
Annals of Hematology (2023)
-
Pre-existing adaptive immunity to the RNA-editing enzyme Cas13d in humans
Nature Medicine (2022)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.