HIV-1 targets L-selectin for adhesion and induces its shedding for viral release

Kononchik, Joseph; Ireland, Joanna; Zou, Zhongcheng; Segura, Jason; Holzapfel, Genevieve; Chastain, Ashley; Wang, Ruipeng; Spencer, Matthew; He, Biao; Stutzman, Nicole; Kano, Daiji; Arthos, James; Fischer, Elizabeth; Chun, Tae-Wook; Moir, Susan; Sun, Peter

doi:10.1038/s41467-018-05197-2

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Published: 19 July 2018

HIV-1 targets L-selectin for adhesion and induces its shedding for viral release

Joseph Kononchik ORCID: orcid.org/0000-0002-6707-5213¹^na1,
Joanna Ireland¹^na1,
Zhongcheng Zou¹,
Jason Segura¹,
Genevieve Holzapfel¹,
Ashley Chastain ORCID: orcid.org/0000-0001-9642-9749¹,
Ruipeng Wang¹,
Matthew Spencer¹,
Biao He¹,
Nicole Stutzman¹,
Daiji Kano¹,
James Arthos²,
Elizabeth Fischer³,
Tae-Wook Chun²,
Susan Moir ORCID: orcid.org/0000-0002-0163-6911² &
…
Peter Sun¹

Nature Communications volume 9, Article number: 2825 (2018) Cite this article

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HIV infections

Abstract

CD4 and chemokine receptors mediate HIV-1 attachment and entry. They are, however, insufficient to explain the preferential viral infection of central memory T cells. Here, we identify L-selectin (CD62L) as a viral adhesion receptor on CD4⁺ T cells. The binding of viral envelope glycans to L-selectin facilitates HIV entry and infection, and L-selectin expression on central memory CD4⁺ T cells supports their preferential infection by HIV. Upon infection, the virus downregulates L-selectin expression through shedding, resulting in an apparent loss of central memory CD4⁺ T cells. Infected effector memory CD4⁺ T cells, however, remain competent in cytokine production. Surprisingly, inhibition of L-selectin shedding markedly reduces HIV-1 infection and suppresses viral release, suggesting that L-selectin shedding is required for HIV-1 release. These findings highlight a critical role for cell surface sheddase in HIV-1 pathogenesis and reveal new antiretroviral strategies based on small molecular inhibitors targeted at metalloproteinases for viral release.

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Introduction

Human immunodeficiency virus type 1 (HIV-1) infection remains a major public health issue. In the absence of an effective vaccine, viral infection can only be managed by highly active antiretroviral treatment (HAART)¹. To date, much of our understanding of HIV-1 entry is based on viral envelope proteins (gp120 and gp41) interacting with CD4 and chemokine receptors^2,3. However, the role of gp120-associated glycans in HIV infection and pathogenesis is less clear. While glycosylations on gp120 shields the virus from humoral immune recognition^4,5, the viral glycans are often recognized by host lectin receptors, such as mannose receptor (MR), DEC-205, and DC-SIGN on macrophage and dendritic cells leading to viral capture and antigen presentation^6,7,8,9. Some of the lectin receptors, such as Siglec receptors on macrophages, are also used by the virus to facilitate its adhesion and infection^10,11. As these lectin receptors are not expressed on CD4⁺ T cells, it is not clear if HIV envelope glycans contribute to the viral infection of T cells despite earlier studies showing mutations in gp120 glycans resulted in replication deficient viruses⁴.

While HIV-1 infects all CD4⁺ T cells, it exhibits a preference for central memory CD4⁺ T cells (T_CM)^12,13,14, and may target them for viral reservoirs^15,16,17,18. L-selectin, also known as CD62L, is a marker for central memory T cells (T_CM). It facilitates lymphocyte rolling adhesion and homing on high endothelial venules (HEV)^19,20. In HIV-1-infected individuals, the number of CD62L⁺ central memory T cells declines as the disease progresses, resulting in dysfunctional immune responses^21,22. Despite the apparent clinical association, the molecular mechanism involving L-selectin in HIV biology is not clear.

Here, we investigated the potential role of L-selectin in HIV-1 infection of T cells. We found that L-selectin, despite its preferential binding to sulfated glycoproteins with sialyl-Lewis x moiety^23,24, recognized gp120-associated glycans, and the binding facilitated the viral adhesion and infection. Unexpectedly, we also found that L-selectin shedding is required for HIV-1 release from infected cells. Current anti-HIV therapies target primarily viral protease, reverse transcriptase, and integrase^25,26. No compounds target viral release. Our findings reveal new pathways for developing antiretroviral treatments targeted at metalloproteinases critical for HIV release.

Results

L-selectin binds to HIV-1 gp120 in solution and on cells

HIV-1 envelope gp120 is highly decorated with N-linked glycans^27,28. While L-selectin is known to recognize HEV-associated O-linked glycans to facilitate lymphocyte rolling adhesion and homing^29,30, it can also bind to certain N-linked glycans in the absence of O-linked glycosylation^23,24. To determine if L-selectin recognized glycosylated gp120, we performed surface plasmon resonance (SPR) binding experiments using recombinant gp120 and soluble human L-selectin (CD62L-Fc). Surprisingly, recombinant gp120 from both R5- (HIV-1_BAL) and X4- (HIV-1_SF33) strains bound to the soluble L-selectin with 50–300 nM affinities (Fig. 1a, Supplementary Figures 1A, 1B). Removal of the N-linked glycans with peptide N-glycosidase F (PNGase F) reduced the binding of both gp120 to DC-SIGN, a C-type lectin receptor known to recognize N-linked gp120 glycans (Fig. 1b). Likewise, the deglycosylation also abolished gp120 binding to L-selectin (Fig. 1b, Supplementary Figure 1C), suggesting the involvement of N-linked gp120 glycans in L-selectin binding. The carbohydrate specificity of the L-selectin and gp120 binding was further examined using an enzyme-linked immunosorbent assay (ELISA) in the presence of EDTA and various competing carbohydrates (Fig. 1c). EDTA and other known L-selectin ligands, such as heparin, fucoidan and sialyl-Lewis x significantly inhibited gp120 binding, consistent with the involvement of the receptor C-type lectin domain in the viral glycan recognition. In addition, sialyllactose but not N-acetylglucosamine or lactose blocked gp120 binding, supporting the involvement of sialyllated N-linked glycans in L-selectin binding¹¹. To investigate if gp120 binds to cell-surface-expressed L-selectin, we conjugated gp120 to fluorescent Qdots and detected their binding to L-selectin-transfected Hela cells (Supplementary Figures 2A, 2C). The gp120-Qdots exhibited specific binding to plate-immobilized recombinant CD4 and L-selectin (Supplementary Figure 1D), and they bound significantly better to L-selectin-transfected than untransfected HeLa cells (Fig. 1d, Supplementary Figures 2B and 2D). As L-selectin is expressed on CD4⁺ T cells and partially colocalized with CD4 (Supplementary Figures 2E–2H), we then incubated gp120-Qdots with CD4⁺ human peripheral blood mononuclear cells (PBMC) in the presence of CD4 or L-selectin blocking antibodies. As expected, the gp120 binding was reduced by the CD4 blocking antibody. Importantly, the gp120 binding was also reduced by the L-selectin blocking antibody, DREG 56, and the binding was further reduced by the combination of CD4 and L-selectin blocking antibodies (Fig. 1e). To further address if L-selectin binds to gp120 on HIV-1 virions, we performed virus capture experiments using either plate-bound soluble L-selectin or L-selectin-transfected 293T cells to capture replication-competent HIV-1_BAL virus. Both plate-bound L-selectin and CD4 captured significantly more HIV-1_BAL than the controls, as measured by p24 ELISA (Fig. 1f). Similarly, more viruses were captured by L-selectin transfected than the untransfected 293T cells (Fig. 1g). As L-selectin is expressed on both CD4⁺ and CD8⁺ T cells, we then examined the binding of HIV-1_BAL virus to CD62L-sorted CD8⁺ T cells from PBMC to avoid the involvement of CD4 (Supplementary Figure 3A). Consistently, the sorted CD62L⁺ CD8⁺ T cells captured significantly more virus than CD62L⁻ CD8⁺ T cells (Fig. 1h). Collectively, these results demonstrated L-selectin recognition of HIV-1 gp120.

L-selectin facilitates HIV viral adhesion and infection

To explore if L-selectin binding affected HIV-1 infection, we transfected human L-selectin cDNA into REV-CEM, a CD4⁺ T-cell line susceptible to X4-tropic HIV infections³¹. Stable transfectants (CD62L-CEM # 2 and #25) expressed significantly more L-selectin but similar levels of CD4, CCR5, and CXCR4 compared to the parental cell line (Supplementary Figure 4). Importantly, the infections of CD62L-CEM #2 and #25 by a replication-competent X4-tropic HIV_LAI were 3-to-4-fold higher than that of the parental CEM cells as measured by intracellular viral capsid p24 staining (Fig. 2a), suggesting that L-selectin facilitated HIV-1 infection. Since the parental cells expressed low levels of L-selectin (Supplementary Figure 4), we further knocked-down CD62L expression using CRISPR/Cas 9 method³². Two clones (CD62L KD #8 and #32) lost L-selectin expressions while the third one (CD62L KD #3) retained the parental level of CD62L expression (Supplementary Figure 4). Despite having comparable CD4 and chemokine receptor expressions, CD62L KD #8 and #32, but not clone #3 were more resistant to HIV_LAI infections than their parental CEM cells (Fig. 2a). Finally, HIV_LAI infection of CD62L KD #8 was reverted back to that of the parental CEM cells by the addition of polybrene (Fig. 2b), a compound known to increase viral-host adhesion by reducing charge repulsion³³, suggesting L-selectin-facilitated viral adhesion.

To examine if L-selectin affected HIV-1 infection of primary CD4⁺ T cells, we carried out HIV-1_BAL infection of CD8-depleted PBMC in the presence of EDTA, a calcium chelator that inhibited L-selectin binding to gp120 (Fig. 1c). While EDTA did not inhibit HIV-1_BAL infection of human macrophages¹¹, it significantly reduced HIV-1_BAL infection of the PBMC (Fig. 2c, Supplementary Figure 3B). Further, DREG-56 significantly inhibited HIV-1_BAL infection of PBMC, particularly at lower viral doses (Fig. 2d), suggesting that L-selectin also facilitated HIV-1 infection of PBMC. To distinguish the contribution of L-selectin to viral entry and replication, we examined viral entry using JRFL- (R5-tropic) and SF33- (X4-tropic) pseudotyped replication deficient viruses. Both JRFL and SF33 infections of PBMC were diminished by the L-selectin blocking antibody (Fig. 3a), and the inhibition was evident over a broad range of viral doses (Fig. 3b, c), supporting the involvement of L-selectin in viral adhesion and entry. To further address the contribution of viral glycans to HIV-1 entry, we produced JRFL and SF33 viruses as well as recombinant SF33 gp120 in HEK 293T or 293S GnTI⁻ cells, the latter is deficient in mature complex N-glycan productions³⁴. Both complex glycan-deficient JRFL and SF33 viruses infected PBMC less than their glycan sufficient counterparts (Fig. 3d), consistent with the solution binding result showing a reduced L-selectin binding by the complex glycan-deficient gp120_SF33 (Supplementary Figure 1E). In addition, removal of the gp120 glycans with PNGase F significantly reduced HIV-1_BAL infection compared to the mock-treatment (Fig. 3e). The importance of viral glycans in HIV-1 infection is further supported by recent findings that cells from glycosylation-deficient individuals had reduced susceptibility to HIV-1 infection³⁵.

HIV-1 infection results in L-selectin shedding

HIV-1 infection is known to downregulate CD4 expression through internalization^36,37. Consistent with published data, HIV-1_BAL infection resulted in a significant loss of CD4 expression in p24⁺ but not in p24⁻ or uninfected T cells on day 6 post-infection (p.i.) and the loss of CD4 was further extended to day 11 p.i. (Fig. 4a−c, Supplementary Figure 3B). Similarly, downregulation in L-selectin expression was also observed in infected, but not uninfected T cells, on days 6 and 11 p.i. (Fig. 4a−c). Indeed, the most significant change in the cell populations associated with HIV-1_BAL infection was the loss of the CD4⁺/CD62L⁺ cells and the gain of the CD4⁻/CD62L⁻ cells (Fig. 4d). These results are consistent with observed downregulation of L-selectin on T cells from HIV-1-infected individuals naïve to HAART³⁸. Comparatively, CCR7 and CD27 expressions were minimally affected in the infected cells (Fig. 4c). L-selectin is known to shed from activated T cells during differentiation of central memory to effector memory T cells to facilitate their migration from lymph nodes to peripheral sites of inflammation³⁹. Crosslinking of CD4 with HIV-1 gp120 induced L-selectin shedding on resting CD4⁺ T cells⁴⁰. To assess if the loss of L-selectin expression in the infected cells resulted from its shedding, we measured the soluble L-selectin levels during HIV-1_BAL infection. The concentration of soluble L-selectin steadily accumulated in the infected but not uninfected supernatants (Fig. 4e, Supplementary Figures 5A−C), supporting the shedding of L-selectin during HIV infection. The progressive loss of the selectin expression in p24⁺ T cells correlated with an increase in viral infection, suggesting that HIV infection-induced L-selectin shedding (Fig. 4f, Supplementary Figure 5D).

L-selectin shedding leads to central memory CD4⁺ T-cell loss

HIV-1 preferentially infects central memory CD4⁺ T cells^14,16, and reduced infections of T_CM are associated with HIV/SIV non-progressors^41,42. Further, the ability to maintain central memory T cells correlates with spontaneous HIV-1 control, and the ability to reconstitute T_CM under HAART indicates a successful response to antiviral treatments^22,43. As a marker of central memory CD4⁺ T cells, L-selectin’s role in promoting HIV adhesion is consistent with T_CM being the preferred target for the viral infection. Indeed, approximately 50% of HIV-1_BAL-infected (p24⁺) T cells were central memory CD45RO⁺/CD27⁺/CD62L⁺ T cells on day 6 post-infection (p.i.), and 10–20% of infected cells were effector memory (T_EM)CD45RO⁺/ CD27⁻/CD62L^+/− cells (Fig. 5a, Fig. Supplementary Figure 5E). Interestingly, the infected p24⁺ T cells contained significantly more transitional memory (T_TM) cells (CD45RO⁺/CD27⁺/CD62L⁻) than the p24⁻ or uninfected controls on day 6 p.i. (Fig. 5a, b), and the T_TM population increased further on day 11 p.i. (Fig. 5a, b). This increase in infected T_TM cells was mirrored by a decrease in the T_CM numbers compared to the uninfected controls, suggesting that the viral infection resulted in the memory CD4⁺ T cells transitioning from T_CM to T_TM. In comparison, a smaller increase in the number of CD45RO⁺/CD27⁻ T_EM cells was observed between the p24⁺ and p24⁻ populations (Fig. 5b). Thus, HIV-induced CD62L shedding likely results in the loss of central memory CD4⁺ T cells in infected individuals.

Previous studies showed that effector memory CD4⁺ T cells from HIV-1-infected individuals are dysfunctional in cytokine productions^44,45. To further investigate the link between HIV infection and the loss of effector memory function, we stimulated HIV-1_BAL-infected PBMC to produce cytokines and measured their production of IFN-γ, TNF-α, and MIP-1β by intracellular staining using flow cytometry. As expected, T_CM consistently produced less cytokines than T_EM cells (Fig. 5c)⁴⁶. However, HIV-1_BAL infection did not result in reduced IFN-γ production compared to uninfected cells (Fig. 5c), regardless of whether it was from central or effector memory T cells. Further, p24⁺ T_CM and T_EM cells consistently secreted more IFN-γ, TNF-α, and MIP-1β than their p24⁻ populations (Fig. 5d), suggesting that acutely infected cells are not dysfunctional in cytokine productions. We speculate that the previously reported T_EM dysfunction may result from grouping of infected T_TM (i.e. the CD62L shed T_CM) as T_EM cells, based on L-selectin expression. Since T_CM produce less cytokines than T_EM cells, this would result in an apparent decrease in cytokine production in T_EM cells. This does not exclude the possibility of dysfunction related to memory T cells exhaustion under persistent chronic HIV infections.

L-selectin shedding is required for HIV-1 viral release

To investigate if HIV-induced L-selectin shedding affects viral pathogenesis, we explored the inhibition of L-selectin shedding. While the proteases responsible for HIV-induced selectin shedding on CD4⁺ T cells remain to be identified, ADAM17 is known to shed L-selectin in response to neutrophil activation and the selectin shedding can be inhibited by Batimastat (BB-94)^47,48,49. Indeed, BB-94 significantly inhibited L-selectin shedding on CD62L-transfected, but not knockdown CEM cells (Fig. 6a). To our surprise, the inhibition of L-selectin shedding, instead of enhancing the viral infection, significantly reduced their susceptibility to HIV_LAI infection (Fig. 6b). Similarly, BB-94 inhibited both X4⁻ and R5-type HIV infection of PBMC (Fig. 6c, d, Supplementary Figure 6A). To confirm BB-94 inhibited L-selectin shedding during the viral infection, we detected the change of cell surface L-selectin expression, the presence of soluble L-selectin in the media, and the presence of a cleaved C-terminal L-selectin transmembrane fragment on cell surface during HIV-1_BAL infection. The results showed that BB-94 prevented HIV-1_BAL-induced downregulation of L-selectin on PBMC, inhibited the accumulation of soluble L-selectin, and suppressed the L-selectin cleavage fragment (Fig. 6e−g, Supplementary Figure 6B). The specificity of BB-94 is demonstrated by contrasting with a related matrix metalloproteinase (MMP) inhibitor, dichloromethylene-diphosphonic acid (DMDP). While both BB-94 and DMDP inhibit the enzymatic cleavage of MMP-1, DMDP did not block the downregulation of L-selectin expression nor its cleavage, and had no effect on HIV-1_BAL infection of PBMC (Supplementary Figures 6C–F). Consistent with the involvement of ADAM17 in HIV-induced L-selectin shedding, the HIV-1_BAL infection of PBMC was significantly inhibited by ADAM17-specific inhibitors TAPI-1 (TNF-α protease inhibitor-1) and TAPI-2 ⁵⁰ (Fig. 6h). Additionally, the viral infection-induced L-selectin cleavage resulted in an identical sized C-terminal fragment as that induced by PMA treatment, known to induce ADAM17 cleavage of L-selectin (Fig. 6g)⁵¹.

To examine if L-selectin shedding affected HIV entry, we infected PBMC with JRFL and SF33 pseudoviruses in the presence of BB-94. In contrast to HIV-1_BAL infection, BB-94 did not affect significantly the entry of JRFL and SF33 (Fig. 6i), suggesting L-selectin shedding affects post entry of the viral infection. Since L-selectin shedding likely occurred late in infection (Fig. 4), we then assessed the effect of BB-94 to HIV release using a trypsin-mediated viral release assay. HIV-1_BAL-infected cells were treated with trypsin on day 6 p.i. to release cell surface-associated viral particles. While BB-94 decreased the amount of virus detected in the supernatant prior to and without trypsin treatment, trypsin treatment resulted in the release of significantly more viral particles in the presence of BB-94 (Fig. 6j), suggesting BB-94 inhibited viral release. To visualize the effect of BB-94 on the viral release, we examined HIV-1_BAL-infected cells using scanning and transmission electron microscopy (SEM/TEM). In the absence of BB-94, the majority of budding viruses from HIV-1_BAL-infected PBMC exhibit typical size of ~100 nm (Fig. 7a, b, e)⁵². In the presence of BB-94, however, more virus-like particles were observed on infected cells, and many of them appeared significantly smaller than the size of budding virions in the absence of BB-94 (Fig. 7c, f, g), suggesting they may be defective viral particles. The retention of budding viruses in the presence of L-selectin shedding inhibitors suggests the selectin shedding is required for viral release. As HIV-1 viruses can infect target cells through cell−cell transfer, we then evaluated if the selectin shedding also affects viral release in cell−cell transfer infections^53,54. When TZM-BL cells were incubated with HIV-1_BAL-infected PBMC, the cell−cell transfer-mediated infection of TZM-BL cells was suppressed by BB-94 in all ratios of PBMC to TZM-BL (Fig. 6k). It is worth noting that BB-94 did not significantly affect VSV infections (Supplementary Figure 6G), suggesting L-selectin shedding impacts some but not all viral infections.

To further investigate if L-selectin shedding is required for HIV release in infected individuals, CD4⁺ T cells from HIV-1-infected viremic and aviremic individuals were stimulated with anti-CD3 to induce viral release. The viremic individuals had viral loads between 1200 and 100,000 copies of HIV-1 RNA/mL, while aviremic individuals were on ART regiments and had undetectable plasma viremia (<40 copies HIV RNA/mL). Consequently, anti-CD3 induced 10–100-fold more virion release from viremic than aviremic CD4⁺ T cells (Fig. 7h). Importantly, the release of HIV from both viremic and aviremic individuals were profoundly inhibited by BB-94, but not by DMDP (Fig. 7h). When the viral release was examined using CD4⁺ T cells from six HIV-1-infected individuals, BB-94 inhibited 30−90% of all viral releases (Fig. 7i). Together, these data suggest that L-selectin shedding is also required for HIV-1 release in vivo. While the mechanism regulating the selectin shedding and HIV-1 release remains to be elucidated, it is possible that failure to shed the selectin results in cell surface retention of budding virions through L-selectin binding to gp120. To assess if a budding virion interacts with L-selectin in the presence of BB-94, we performed immuno-TEM on HIV-1_LAI-infected Rev-CEM to observe budding virions in the presence of immunogold-conjugated anti-CD62L. While gold particles were readily found on infected cells, they were generally not associated with budding virions (Fig. 8a, b). In the presence of BB-94, however, a significant number of gold particles were associated with budding virions at the budding focal point (Fig. 8c, d), suggesting the presence of selectin tethering (Fig. 9).

Discussion

L-selectin (CD62L) provides rolling adhesion for lymphocyte extravasation to secondary lymph nodes and sites of inflammation²⁰. The binding of HIV-1 glycans to L-selectin can be viewed similarly as viral rolling adhesion on CD4⁺ T cells to facilitate the binding to CD4 and other coreceptors, and thus to enhance viral infections. Biochemically, the recognition of gp120 represents a novel function for L-selectin in addition to the binding of O-linked glycans. While L-selectin prefers sialyl-Lewis x type of O-linked glycans, its binding to gp120 is likely the result of engaging multiple low affinity N-linked glycans on heavily glycosylated envelope proteins. The carbohydrate promiscuity of L-selectin is supported by a recent crystal structure of L-selectin binding to a mannose moiety on an N-linked glycosylation⁵⁵. Importantly, we demonstrated the involvement of L-selectin in HIV-1 viral entry and infection. Thus, HIV viral envelope glycans not only shield the virus from host immune detection, but also provide necessary adhesion to macrophages and CD4⁺ T cells for viral entry and infection^10,11. It is worth noting that since the viral glycan-mediated L-selectin binding depends primarily on viral envelope glycosylations, other viruses with highly glycosylated envelope proteins may also bind L-selectin to facilitate their adhesion and entry.

Early experiments suggested the ability of HIV-1 envelope to induce L-selectin shedding on resting CD4⁺ T cells⁴⁰, we showed here that HIV-1 infection resulted in a progressive loss in L-selectin expression. Further, the downregulation of L-selectin expression was significantly more in p24⁺ than p24⁻ populations suggesting that the selectin shedding was induced by productive viral infection rather than gp120 binding. While shedding of L-selectin on HIV-1-infected T cells may deter additional virions from binding to an already infected cell, preventing so called “super-infection”, the inhibition of shedding did not significantly affect the viral entry. Instead, it resulted in the retention of virus-like particles on infected cells. Strikingly, many of the budding virus-like particles appeared smaller than fully formed viruses in the presence of BB-94, suggesting they are defective virions.

In summary, we showed here a direct involvement of L-selectin in HIV-1 attachment to CD4⁺ T cells to facilitate the viral entry. The L-selectin-mediated viral adhesion explains the preferential infection of central memory CD4⁺ T cells by HIV-1 virus. Upon infection, however, HIV induces L-selectin shedding by ADAM17 family of metalloproteinases from infected T cells to facilitate the viral release (Fig. 9). It remains unknown which viral genes induce L-selectin shedding. While HIV-1 encoded nef was shown important for CD4 downregulation⁵⁶, it would be interesting to see if nef, vpu, or other viral encoded proteins are responsible for activating L-selectin shedding. We speculate that the involvement of L-selectin in HIV-1 adhesion and release is not unique to HIV; rather, the underline mechanism can be generalized to all lectin receptors and their involvement in the adhesion and release of any glycosylated envelope viruses. Despite the success of HAART in reducing viral load in HIV-infected individuals, a cure remains elusive in the absence of an effective vaccine. The success of HAART in suppressing plasma HIV-1 viremia has brought renewed focus on finding and eliminating latently infected viral reservoirs through their reactivation followed by elimination with HAART therapy^{57,58,59,60,61,62}. Our finding that L-selectin shedding is required for HIV release either in experimental infections or from patient-derived CD4⁺ T cells reveals a novel pathway to suppress both active and latent viral release. While the active sheddase on infected CD4⁺ T cells remain to be identified, small molecular inhibitors of metalloproteinases can be explored as a new class of antiviral compounds targeted at HIV release.

Methods

Reagents

Unless otherwise specified, all reagents and chemicals were purchased from Sigma-Aldrich Co. (St. Louis, MO). Recombinant protein was purchased from R&D Systems, Inc. (Minneapolis, MN). Blocking antibody against CD62L (DREG-56) was harvested from hybridoma cells in serum-free media from Invitrogen (Carlsbad, CA) and purified using a Protein A column or purchased from eBioscience (San Diego, CA). Unlabeled mouse antihuman CD4 monoclonal antibody (RPA-T4), CD28 were obtained from eBioscience (San Diego, CA). Antihuman CD3 antibody (OKT3) was kindly provided by Dr. Gilliland of Johnson & Johnson. Fluorescently labeled antibodies for flow cytometry against CD14, CXCR4, CCR5, CD8, CD4, CD3, CD62L, CD27, CD45RO, CCR7, IFNγ and their isotype controls (IgG1, IgG2A, IgG2B) were obtained from BD Biosciences (San Jose, CA), BioLegend (San Diego, CA) or eBioscience (San Diego, CA). Alexa-647-labeled antibodies used for confocal microscopy were obtained from BioLegend (San Diego, CA). HIV-1 core antigen antibody (KC57-FITC) for intracellular p24 staining was purchased from Beckman Coulter, Inc. (Miami, FL). Interleukin 2 (IL-2) was obtained from Peprotech Inc. (Rocky Hill, NJ). Polyacrylamide (PAA)-conjugated model carbohydrates were obtained from Glycotech, Inc. (Rockville, MD). All other carbohydrates were purchased from Carbosynth Ltd. (Compton, Berkshire, UK). Recombinant gp120 proteins were expressed either using stably transfected CHO cells or transiently transfected 293T and 293S GnTI⁻ cells in monomeric forms⁶³. The Luciferase Assay System was purchased from Promega Corporation (Madison, WI). HIV-1 p24 ELISA kit was obtained from PerkinElmer Life Sciences, Inc. (Waltham, MA). Ficoll-Paque was purchased from GE Healthcare Life Sciences (Pittsburgh, PA). For FACS analysis, recombinant gp120 proteins were labeled with biotin using a biotinylation kit from Pierce Biotechnology (Rockford, IL). RPMI, penicillin/streptomycin, fetal bovine serum (FBS), HEPES, and Versene were purchased from Invitrogen Corporation (Carlsbad, CA). The metalloproteinase inhibitor, BB94 (Batimastat), TAPI-1 (TNFα protease inhibitor-1), and TAPI-2 were purchased from Santa Cruz Biotechnology.

Activation of peripheral blood mononuclear cells

Peripheral blood mononuclear cells were isolated by Ficoll-Paque gradient from randomly selected nonidentified healthy donors. The use of human PBMC is approved by the Department of Transfusion Medicine at the Clinical Center of National Institutes of Health. The isolated PBMC were distributed at 3×10⁶/mL in 12-well plates with RPMI supplemented with 10% FBS, 1% penicillin/streptomycin and 20 U/mL IL-2, activated with 1–2 µg/mL anti-CD3 antibody Okt3 for 48 h. CD8⁺ cell depletion was completed using the StemCell (Vancouver, BC, Canada) EasySep™ Human CD8 Positive Selection Kit prior to infection. Total cell count and viability determinations were assessed with the Guava Personal Cell Analysis System (Millipore) or the Luna FL Dual Fluorescence Cell Counter (Logos Biosystems); all assays were performed with a cellular viability greater than 90%. All data presented in this manuscript were results of individual typical experiments derived from at least two independent experiments.

Stable CD62L-transfected HeLa, 293T, and Rev-CEM cells

HeLa cells were cultured in DMEM/F12 medium supplemented with 5% FBS. Neomycin-resistant pEX02-CD62L vector (Genecopeia Inc., Rockville MD) containing coding regions for human CD62L were transfected into HeLa cells using Lipofectamine 2000 from Invitrogen (Carlsbad, CA), and cultured at 37 °C and 5% CO₂ in DMEM/F12 supplemented with 5% FBS, 1% penicillin/streptomycin, and 300 µg/mL G418. G418-resistant cells were sorted on an FACS Aria II (BD Biosciences) for CD62L expression. The sorted populations were expanded in the same growth media. To reduce cleavage of selectins from the cell surface, sorted and unsorted transfected cells were grown in Nunc™ UpCell^® six-well plates from Thermo Fisher Scientific (Waltham, MA) and released after incubating at room temperature in Versene and gentle pipetting. Rev-CEM cells³¹, obtained from the NIH AIDS Research and Reference Reagent Program (https://www.aidsreagent.org/), were transfected with 2 µg of full-length human L-selectin cDNA in pEX02 vector using Nucleofector^® Kit V (Lonza, Walkersville, MD). Two days later, cells were selected with 400 µg/mL of G418 and continue to culture for 2 weeks, and then sorted for CD62L expression. Individual clones were screened for CD62L expressions and high expression clones were expanded for infection experiments. CD62L knockout CEM cell lines were established similarly, except SELL CRISPR guide RNA 6 vector was transfected into the parental Rev-CEM cells and cells were selected with 1 µg/mL of puromycin (GeneScript, Piscataway, NJ)³². Resistant cells were sorted for the lack of CD62L expression and maintained in RPMI-1640 media supplemented with 10% FBS, 1% penicillin/streptomycin, and 1 µg/ml puromycin.

Preparation of the pseudotyped HIV viruses

The HIV viral vector, pNL4-3.Luc.R-E-, which contains the firefly luciferase gene inserted into the NL4-3 HIV nef gene and frameshift mutations to render it E-, was used to generate all pseudotyped viruses⁶⁴. In brief, the expression vectors for pNL4-3.Luc.R-E-, the amphotropic envelope pHEF-VSVG, and the R5-tropic HIV JRFL envelope were obtained through the NIH AIDS Research and Reference Reagent Program. Expression vectors for the X4-tropic SF33 HIV-1 envelope was obtained from M. Martin⁶⁵. Recombinant HIV luciferase viruses were generated by cotransfecting 293T cells with 5 µg of the NL4-3 backbone and either 5 µg of the HIV envelopes or 1.5 µg of the VSV envelope, as previously described⁶⁶. Virus collected in the culture supernatant were quantified by HIV p24 ELISA and adjusted to 1 mg/mL p24. Pseudotyped virus deficient for complex carbohydrates were generated similarly except with a transfected HEK 293S GNTI⁻ cells, obtained from American Type Culture Collection (Manassas, VA). Activated CD8-depleted PBMC (CD8⁻ PBMC) were infected with equal amounts of pseudotyped HIV-1 viruses, as measured by their p24 concentration.

Replication competent virus

The R5-tropic BAL strain of HIV-1 (HIV-1_BAL), propagated using primary human macrophages, was purchased from Advanced Biotechnologies Inc. (Columbia, MD). The X4-tropic HIV-1 LAI was obtained from the NIH AIDS Research and Reference Reagent Program (https://www.aidsreagent.org/). Both viruses were propagated by infecting CD8⁻ PBMC. Day 6 supernatant was harvested and 200 µL aliquots were frozen. Viral titers in TCID₅₀ were determined by titrating viral infection in CD8⁻ PBMC and measuring p24 ELISA using PerkinElmer ALLIANCE HIV-I p24 ELISA kit (PerkinElmer Life Sciences, Inc., Waltham, MA).

BIAcore, ELISA binding, and viral capture experiments

Surface plasmon resonance measurements were performed using a BIAcore 3000 instrument (GE Healthcare Life Sciences). Recombinant CD62L⁻Fc, DC-SIGN Fc fusion proteins (R&D Systems, Inc.), or anti-gp120 antibody 2G12 were immobilized onto either CM5 or C1-sensor chips by N-hydrosuccinimide/1-ethyl-3(-3-dimethylaminopropyl) carbodiimide hydrochloride(NHS/EDC) crosslinking in sodium acetate buffer at pH5.0. Human IgG₁ was immobilized as control to Fc fusion proteins. Binding assays were performed in HBS-P buffer (10 mM HEPES pH 7.2, 150 mM NaCl, 0.005% P20) (GE Healthcare Life Sciences), supplemented with 0.5–2 mM CaCl₂, and 0.5 mg/mL bovine serum albumin (BSA) to reduce nonspecific binding. Recombinant gp120 proteins with varying concentrations between 0.003 and 2 µM, in the HBS-P + Ca buffer, were injected over immobilized receptors. To deglycosylate gp120, 50 µg of gp120 was treated with 100 units PNGaseF (New England Biolabs) under nondenaturing conditions for 3 h at 37 °C following the manufacturer’s guide, and then dialyzed overnight in HBS-P binding buffer. The dissociation constants (K_D) were determined from kinetic curve fitting using the BIAevaluation software on blank subtracted sensorgrams (GE Healthcare Life Sciences). To detect gp120 and CD62L binding by ELISA, 50 ng of gp120 proteins were immobilized in individual wells of a 96-well plate for 1 h at room temperature in coating buffer (10 mM Tris [pH7.5] and 2 mM CaCl₂), blocked for 1 h using blocking buffer (10 mM Tris [pH7.5], 0.1% Tween20), and washed three times with the same buffer. CD62L-Fc (25 ng) was added to each well in the presence or absence of various inhibitors (10 mM EDTA or 10 mg/mL carbohydrates) together with a goat antihuman IgG-HRP secondary antibody for 1 h at room temperature. The plate was washed five times and readout was colorimetric using a TMB substrate and analyzed on a SpectraMax Plus 384 spectrophotometer (Molecular Devices). All statistics are calculated using unpaired Student’s t test.

To perform viral capture ELISA, 96-well plates were coated overnight at room temperature with 10 µg protein per well of CD4, CD62L-Fc, BSA, IgG1 (R&D Systems) or PBS. The wells were washed with ELISA wash buffer (PerkinElmer ALLIANCE HIV-I P24 ELISA), and blocked for 1 h at room temperature with 1% BSA in 1× PBS. HIV-1_BAL was added to each well at a 1:1000 dilution of virus for 1 h at 37 °C in the presence or absence of 5 mM EDTA. The wells were gently washed with PBS, and Triton X was added to denature the bound virus. The amount of captured virus was determined using the PerkinElmer p24 ELISA kit. To capture HIV-1_BAL by CD62L-transfected 293T cells or CD62L-sorted CD8⁺ T cells, HIV-1_BAL virus (1:1000 dilution) were mixed with 10⁶ CD62L-transfected or untransfected 293T cells, sorted CD62L⁺ or CD62L⁻ CD8⁺ T cells at m.o.i. of ~0.1, incubated for 1 h at 37 °C. The cells were gently washed with PBS, treated with Triton X. The amount of cell-bound virus was determined as above.

Single-round infection assay

Stimulated CD8⁻ PBMC were resuspended at 2×10⁶/mL in culture media. Aliquots of 200 µL (4×10⁵ cells) were transferred to 96-well plates for incubation in triplicate with anti-CD4 (10 µg/mL), anti-CD62L (30 µg/mL), or isotype (30 µg/mL) antibody at 37 °C for 60 min prior to the addition of virus. Luciferase viruses pseudotyped with envelopes from JRFL (R5-tropic) and SF33 (X4-tropic) HIV-1 and VSV were added to the cells at a concentration of 100 ng/mL HIV p24. The infected CD8⁻ PBMC were then incubated for 72 h, lysed, and assayed for luciferase activity according to the manufacturer’s recommendations (Promega Corporation, Madison, WI). Pseudotyped virus from GNTI⁻ cells were added at 100 ng/mL to cells as above.

Infection with replication-competent viruses

Rev-CEM cells expressing differing levels of CD62L were infected with 1:100 v/v dilution of HIV-1_LAI at 2×10⁵ cells/mL in a 48-well plate along with uninfected controls at 37 °C with 5.5% CO₂. Media was replaced every 3 days post-infection. For infection of PBMC, CD8-depleted PBMC were resuspended at 2×10⁶/mL in culture media. Cells (1 mL) were incubated with antibodies or inhibitors at 37 °C for 60 min prior to infection. The concentrations used were: 10 µg/mL anti-CD4, 30 µg/mL anti-CD62L, 10 or 30 µg/mL isotype antibody in matching concentration, 1 µg/mL polybrene, 5 mM EDTA and 25–100 µM BB-94. Cells were exposed to HIV-1_BAL (~4×10⁵ TCID₅₀) at 1:5000 dilution for 1 h at 37 °C followed by washing with 10 mL culture media. Culture supernatants were sampled and wells were replenished with fresh media on days 6 or 7, or otherwise indicated days p.i. Infections were detected by either intracellular p24 at indicated time points or real-time PCR for viral DNA copy number at day 3 post infections. Intracellular p24 levels were measured on viable CD3⁺ populations using FITC-conjugated KC57 antibody using the Cytofix/Cytoperm kit from BD Biosciences (San Jose, CA). Samples were collected on an FACSCanto II (BD Biosciences). Real-time PCR was assayed as described previously⁶⁷. All statistical analyses were carried out using the software Prism 7 (GraphPad Software, Inc.). The inhibitors were added 30 min before infection and were replenished after the postinfection wash and subsequent media exchanges. The concentrations of soluble L-selectin in supernatants of infected and uninfected samples were quantified using the human L-selectin DuoSet ELISA kit (R&D Systems) and normalized to per day accumulation. To detect the protease cleaved 6 kD C-terminal membrane-bound fragment of CD62L, western blot analyses were performed similar to previously reported⁵¹. In brief, equal amount of cell lysates from 10⁷ HIV-infected or uninfected PBMC in the presence of BB-94, DMDP, or control DMSO were incubated with 1 μg Dynabeads-conjugated anti-CD62L cytoplasmic domain antibody (RayBiotech, cat. no. 119-17273) for 20 min. After three washes, the protein is eluted for SDS-PAGE, and blotted onto a PVDF membrane. The membrane is blocked with 5% nonfat milk for 2 h and then incubated with the anti-CD62L cytoplasmic domain antibody for overnight. After washes, the membrane is probed with HRP-conjugated goat anti-rabbit antibody for 1 h, and visualized with chemiluminescent reagents. As positive controls for the cleaved 6 kD CD62L fragment, uninfected PBMC were activated with Phaseolus vulgaris agglutinin (PHA) for 5 days and treated with phorbol 12-myristate 13-acetate (PMA) for 30 min to induce L-selectin shedding. For trypsin-treated viral release, PBMC were infected with HIV-1_BAL in the presence and absence of BB-94. On day 6 of the viral infection, supernatants were collected, and the infected cells were washed with PBS and treated with 1× cell culture trypsin-EDTA solution or media for 15 min. The amount of virus released was determined by p24 ELISA.

PNGase treatment of activated CD8-depleted PBMC

HIV-1_BAL virus was diluted to 1:5000 in RPMI 1640 containing 0.5% FBS. The virus was treated with 20,000 U PNGase/mL or mock for 1 h at 37 °C. Activated CD8-depleted PBMC were resuspended at 2×10⁶/mL and infected with either the PNGase-treated, or mock-treated virus for 1 h at 37 °C as described above. The CD8⁻ PBMC were then washed with RPMI 1640 containing 10% FBS and resuspended at 2×10⁶ cells/mL and plated in a 48-well plate. Infection and analysis as described above.

Central memory CD4 T-cell staining

On day 6 or 11 p.i., cells were harvested and stained with T-cell memory surface markers including CD3, CD4, CD27, CD45RO, CD62L, CCR7, or the appropriate isotype controls. Cells were washed, permeabilized using the BD Cytofix/Cytoperm kit (BD Biosciences) according to the manufacturer’s instructions and stained for intracellular p24. Samples were acquired on an FACSCanto II and analyzed using FlowJo software (FlowJo, LLC; Ashland, OR). The covariance between T_CM and T_TM was calculated using two-way ANOVA for p24+ and p24− populations.

Stimulation for IFNγ, TNF-α, and MIP-1β production

On day 6 p.i., CD8-depleted PBMC were stimulated for 6 h with Leukocyte Activation Cocktail (BD Biosciences) at 37 °C. Cells were stained for memory cell markers as above, permeabilized with the BD Cytofix/Cytoperm Kit, then washed and stained for intracellular p24, IFNγ, TNF-α, and MIP-1β. Samples were acquired on an FACSCanto II and analyzed using FlowJo software.

Cell−cell transfer infections

For the cell−cell transfer-mediated infection, TZM-BL cells were seeded in a 96-well, flat-bottom plate at 3000 cells/well 3 days before the assay. PBMC infected with HIV-1_BAL for 3 days with and without the presence of BB-94 were added to the wells at the concentration of 80×10³, 40×10³, 20 × 10³, 10 × 10³, and 5 × 10³ cells/well in equal volume. Fresh BB-94 was added to cells that received BB-94 treatment for the cell−cell transfer assay. All conditions were prepared in triplicate. The cell mixtures were incubated at 37 °C for 3 days, followed by lysis and measurement of the subsequent luciferase expression as per the manufacturer’s instructions.

HIV-1 release assay

PBMC were obtained by leukapheresis and ficoll-hypaque centrifugation. CD4+ T cells were isolated using a cell separation system (StemCell Technologies). Cells were cultured with medium alone or with plate-bound anti-CD3 and soluble anti-CD28 antibody in the absence (DMSO) or presence of BB-94 in duplicate for 48 h. The copy number of virion-associated HIV RNA in the above cell culture supernatants was determined using the Cobas Ampliprep/Cobas Taqman HIV-1 Test, Version 2.0 (Roche Diagnostics). The limit of detection for this system is 20 copies/mL.

Confocal microscopy

CD4+ T cells were prepared from isolated PBMC using the StemCell EasySep™ Human CD4+T Cell Enrichment Kit. Isolated CD4+ cells were then spun onto Superfrost glass slides using a CytoSpin 3 and CytoSep funnels (Thermo Fisher Scientific, Waltham, MA) at 1000 rpm for 3 min followed by fixation in 90% methanol and stained in 1× PBS with 10% FBS and 0.03% NaN₃. Alexa-647-labeled CD62L antibody and FITC-, PE- or biotin-labeled CD4 antibody were used in a 1:250 dilution for 15 min followed by two washes. For biotin-labeled slides streptavidin-conjugated Alexa-405 was added to the staining mix. Labeled slides were mounted with ProLong^® Gold Antifade Reagent (Life Technologies, Grand Island, NY) and sealed after 24 h of curing with nail polish. Images were captured on a Zeiss LSM 780 AxioObserver confocal microscope. A 405 nm diode laser and 633 nm diode laser were used to excite Alexa-405-conjugated CD4 antibody and Alexa-647-conjugated CD62L antibody, respectively.

gp120-Qdot binding assay

gp120-QDots were prepared by mixing 12-fold molar excess of monomeric gp120 to Qdot^® 625 ITK™ carboxyl quantum dots (Thermo Fisher Scientific) with EDC and NHS in 1× PBS with 10 mM HEPES. The gp120 to Qdot ratio is chosen to be similar to the number of envelop trimers observed on a virion⁶⁸. After 2 h at room temperature and overnight at 4 °C, the reaction is quenched with 1 M Tris (final concentration 300 mM) and stored at 4 °C until used. The final concentration used in the assay was 27pM. Full conjugation of gp120 to the Qdots was examined by SDS-PAGE using the Pierce Silver Stain Kit from Thermo Fisher Scientific (Waltham, MA). Anti-CD4 (RPA-T4), anti-CD62L (DREG-56), and isotype (IgG1) were used at 10, 30, and 10 µg/mL, respectively, as in all other experiments. The prepared gp120-Qdots bound specifically to plate-immobilized soluble L-selectin and CD4 (Fig S1D).

Mock or CD62L-transfected HeLa cells were transferred to ethanol-cleaned eight-well glass coverslip chambers and allowed to adhere for 16−48 h before used. Qdots were added to the HeLa cells and allowed to equilibrate before imaging. Images were collected on an Olympus IX-81 microscope adapted for Total Internal Reflection Fluorescence (TIRF) imaging. A 405 nm diode laser was used to excite Qdots and emission was filtered with a 605/40 band-pass filter before imaging on a Cascade IIB 1024EM CCD camera. Image stacks were deconvoluted with a measured PSF in Huygens Essential software by Scientific Volume Imaging (Hilversum, Netherlands) followed by Qdot recognition and quantification using FIJI imaging software⁶⁹ and its 3D object counter. For immobilized protein binding, 100 ng of soluble CD4 or CD62L were adsorbed overnight at room temperature to eight-well sterile glass coverslip chambers. The wells were washed twice before the addition of QDots in 1× PBS containing 10% FBS. After room temperature equilibration for at least 10 min, the QDots were imaged as above.

Transmission and scanning electron microscopy

Specimens for TEM were fixed with 2.5% glutaraldehyde in 0.1 M Sorenson’s buffer. Samples were post-fixed 1 h with 0.5% osmium tetroxide/0.8% potassium ferricyanide, 1 h with 1% tannic acid and overnight with 1% uranyl acetate at 4 °C. Samples were dehydrated with a graded ethanol series, and embedded in Spurr’s resin. Thin sections were cut with a Leica UCT ultramicrotome (Vienna, Austria) stained with 1% uranyl acetate and Reynold’s lead citrate prior to viewing at 80 kV on a Hitachi 7500 transmission electron microscope (Hitachi-High Technologies, Tokyo, Japan). Digital images were acquired with an AMT digital camera system (AMT, Chazy, NY). For immunogold labeling, ~10⁶ cells were washed with 2 ml PBS, centrifuged at 250 × g for 5 min, resuspended in 100 µL of labeling buffer (PBS with 1% BSA), and incubated with 10 µg of mouse antihuman CD62L (clone FMC46, Thermo Fisher) for 1 h on ice. After removal of the primary antibody through a cycle of wash, cells were incubated with 10 µg of secondary goat anti-mouse IgG H&L conjugated to 10 nm gold (Electron Microscopy Sciences) for 1 h on ice, then washed and fixed in 2.5% glutaraldehyde with 0.1 M sodium cacodylate at pH 7.4. For SEM, cells were adhered to silicon chips and fixed with 2.5% glutaraldehyde in 0.1 M Sorenson’s buffer overnight at 4 °C. Specimens were post-fixed for 1 h with 1% osmium tetroxide and dehydrated in a graded ethanol series. The samples were critical-point dried under CO₂ in a Bal-Tec model cpd 030 dryer (Balzers, Liechtenstein), mounted on aluminum studs, and sputter-coated with 75 angstroms of iridium in a model IBS/TM200S ion beam sputter coater (South Bay Technologies, San Clemente, California). Specimens were viewed at 5 kV in a Hitachi SU-8000 field emission SEM (Hitachi-High Technologies, Tokyo, Japan) using secondary imaging mode. Virion-like particles were identified in SEM images as 50–150 nm size cell surface-associated spherical particles, excluding nodules appearing at the tip of filopodia. Virion-like particles in TEM were further characterized by the presence of capsid. The uninfected samples showed less than ten virion-like particles per image using this criteria.

Data availability

All data generated or analyzed during this study are available upon request.

References

Martin, A. R. & Siliciano, R. F. Progress toward HIV eradication: case reports, current efforts, and the challenges associated with cure. Annu. Rev. Med. 67, 215–228 (2016).
Article PubMed CAS Google Scholar
Berger, E. A., Murphy, P. M. & Farber, J. M. Chemokine receptors as HIV-1 coreceptors: roles in viral entry, tropism, and disease. Annu. Rev. Immunol. 17, 657–700 (1999).
Article PubMed CAS Google Scholar
Wu, L. et al. CD4-induced interaction of primary HIV-1 gp120 glycoproteins with the chemokine receptor CCR-5. Nature 384, 179–183 (1996).
Article ADS PubMed CAS Google Scholar
Reitter, J. N. & Desrosiers, R. C. Identification of replication-competent strains of simian immunodeficiency virus lacking multiple attachment sites for N-linked carbohydrates in variable regions 1 and 2 of the surface envelope protein. J. Virol. 72, 5399–5407 (1998).
PubMed PubMed Central CAS Google Scholar
Quinones-Kochs, M. I., Buonocore, L. & Rose, J. K. Role of N-linked glycans in a human immunodeficiency virus envelope glycoprotein: effects on protein function and the neutralizing antibody response. J. Virol. 76, 4199–4211 (2002).
Article PubMed PubMed Central CAS Google Scholar
Trujillo, J. R. et al. Noninfectious entry of HIV-1 into peripheral and brain macrophages mediated by the mannose receptor. Proc. Natl. Acad. Sci. USA 104, 5097–5102 (2007).
Article ADS PubMed CAS Google Scholar
Bozzacco, L. et al. DEC-205 receptor on dendritic cells mediates presentation of HIV gag protein to CD8+ T cells in a spectrum of human MHC I haplotypes. Proc. Natl. Acad. Sci. USA 104, 1289–1294 (2007).
Article ADS PubMed CAS Google Scholar
Geijtenbeek, T. et al. DC-SIGN, a dendritic cell-specific HIV-1-binding protein that enhances trans-infection of T cells. Cell 100, 587–597 (2000).
Article PubMed CAS Google Scholar
Snyder, G. A. et al. Characterization of DC-SIGN/R interaction with human immunodeficiency virus type 1 gp120 and ICAM molecules favors the receptor’s role as an antigen-capturing rather than an adhesion receptor. J. Virol. 79, 4589–4598 (2005).
Article PubMed PubMed Central CAS Google Scholar
Rempel, H., Calosing, C., Sun, B. & Pulliam, L. Sialoadhesin expressed on IFN-induced monocytes binds HIV-1 and enhances infectivity. PLoS ONE 3, e1967 (2008).
Article ADS PubMed PubMed Central CAS Google Scholar
Zou, Z. et al. Siglecs facilitate HIV-1 infection of macrophages through adhesion with viral sialic acids. PLoS ONE 6, e24559 (2011).
Article ADS PubMed PubMed Central CAS Google Scholar
Ostrowski, M. et al. Both memory and CD45RA+/CD62L+ naive CD4(+) T cells are infected in human immunodeficiency virus type 1-infected individuals. J. Virol. 73, 6430–6435 (1999).
PubMed PubMed Central CAS Google Scholar
Douek, D. C. et al. HIV preferentially infects HIV-specific CD4+T cells. Nature 417, 95–98 (2002).
Article ADS PubMed CAS Google Scholar
Schnittman, S. M. et al. Preferential infection of CD4+ memory T cells by human immunodeficiency virus type 1: evidence for a role in the selective T-cell functional defects observed in infected individuals. Proc. Natl. Acad. Sci. USA 87, 6058–6062 (1990).
Article ADS PubMed CAS Google Scholar
Chomont, N. et al. HIV reservoir size and persistence are driven by T cell survival and homeostatic proliferation. Nat. Med. 15, 893–900 (2009).
Article PubMed PubMed Central CAS Google Scholar
Lambotte, O. et al. Persistence of replication-competent HIV in both memory and naive CD4 T cell subsets in patients on prolonged and effective HAART. AIDS 16, 2151–2157 (2002).
Article PubMed CAS Google Scholar
Hua, W. et al. Central memory CD4 cells are an early indicator of immune reconstitution in HIV/AIDS patients with anti-retroviral treatment. Immunol. Invest. 41, 1–14 (2012).
Article PubMed CAS Google Scholar
Hong, F. F. & Mellors, J. W. Impact of antiretroviral therapy on HIV-1 persistence: the case for early initiation. AIDS Rev. 17, 71–82 (2015).
PubMed Google Scholar
von Andrian, U. H. Intravital microscopy of the peripheral lymph node microcirculation in mice. Microcirculation 3, 287–300 (1996).
Article Google Scholar
Sallusto, F., Geginat, J. & Lanzavecchia, A. Central memory and effector memory T cell subsets: function, generation, and maintenance. Annu. Rev. Immunol. 22, 745–763 (2004).
Article PubMed CAS Google Scholar
Letvin, N. L. et al. Preserved CD4+ central memory T cells and survival in vaccinated SIV-challenged monkeys. Science 312, 1530–1533 (2006).
Article ADS PubMed PubMed Central CAS Google Scholar
Potter, S. J. et al. Preserved central memory and activated effector memory CD4+ T-cell subsets in human immunodeficiency virus controllers: an ANRS EP36 study. J. Virol. 81, 13904–13915 (2007).
Article PubMed PubMed Central CAS Google Scholar
Clark, R., Fuhlbrigge, R. & Springer, T. L-Selectin ligands that are O-glycoprotease resistant and distinct from MECA-79 antigen are sufficient for tethering and rolling of lymphocytes on human high endothelial venules. J. Cell Biol. 140, 721–731 (1998).
Article PubMed PubMed Central CAS Google Scholar
Mitoma, J. et al. Critical functions of N-glycans in L-selectin-mediated lymphocyte homing and recruitment. Nat. Immunol. 8, 409–418 (2007).
Article PubMed CAS Google Scholar
Pan, C., Liu, S. & Jiang, S. HIV-1 gp41 fusion intermediate: a target for HIV therapeutics. J. Formos. Med. Assoc. 109, 94–105 (2010).
Article PubMed CAS Google Scholar
Liu, S. & Jiang, S. High throughput screening and characterization of HIV-1 entry inhibitors targeting gp41: theories and techniques. Curr. Pharm. Des. 10, 1827–1843 (2004).
Article PubMed Google Scholar
Doores, K. J. et al. Envelope glycans of immunodeficiency virions are almost entirely oligomannose antigens. Proc. Natl. Acad. Sci. USA 107, 13800–13805 (2010).
Article ADS PubMed Google Scholar
Pancera, M. et al. Structure and immune recognition of trimeric pre-fusion HIV-1 Env. Nature 514, 455–461 (2014).
Article ADS PubMed PubMed Central CAS Google Scholar
Poppe, L., Brown, G. S., Philo, J. S., Nikrad, P. V. & Shah, B. H. Conformation of sLe(x) tetrasaccharide, free in solution and bound to E-, P-, and L-selectin. J. Am. Chem. Soc. 119, 1727–1736 (1997).
Article CAS Google Scholar
Klopocki, A. G. et al. Replacing a lectin domain residue in L-selectin enhances binding to P-selectin glycoprotein ligand-1 but not to 6-sulfo-sialyl Lewis x. J. Biol. Chem. 283, 11493–11500 (2008).
Article PubMed PubMed Central CAS Google Scholar
Wu, Y., Beddall, M. H. & Marsh, J. W. Rev-dependent indicator T cell line. Curr. HIV Res. 5, 394–402 (2007).
Article PubMed PubMed Central CAS Google Scholar
Sanjana, N. E., Shalem, O. & Zhang, F. Improved vectors and genome-wide libraries for CRISPR screening. Nat. Methods 11, 783–784 (2014).
Article PubMed PubMed Central CAS Google Scholar
Toyoshima, K. & Vogt, P. K. Enhancement and inhibition of avian sarcoma viruses by polycations and polyanions. Virology 38, 414–426 (1969).
Article PubMed CAS Google Scholar
Reeves, P. J., Callewaert, N., Contreras, R. & Khorana, H. G. Structure and function in rhodopsin: high-level expression of rhodopsin with restricted and homogeneous N-glycosylation by a tetracycline-inducible N-acetylglucosaminyltransferase I⁻negative HEK293S stable mammalian cell line. Proc. Natl. Acad. Sci. USA 99, 13419–13424 (2002).
Article ADS PubMed CAS Google Scholar
Sadat, M. A. et al. Glycosylation, hypogammaglobulinemia, and resistance to viral infections. N. Engl. J. Med. 370, 1615–1625 (2014).
Article PubMed PubMed Central CAS Google Scholar
Garcia, J. V. & Miller, A. D. Serine phosphorylation-independent downregulation of cell-surface CD4 by nef. Nature 350, 508–511 (1991).
Article ADS PubMed CAS Google Scholar
Guy, B. et al. HIV F/3’ orf encodes a phosphorylated GTP-binding protein resembling an oncogene product. Nature 330, 266–269 (1987).
Article ADS PubMed CAS Google Scholar
Vassena, L. et al. Brief report: L-Selectin (CD62L) Is downregulated on CD4+ and CD8+T lymphocytes of HIV-1-infected individuals naive for ART. J. Acquir. Immune Defic. Syndr. 72, 492–497 (2016).
PubMed CAS Google Scholar
Galkina, E. et al. L-selectin shedding does not regulate constitutive T cell trafficking but controls the migration pathways of antigen-activated T lymphocytes. J. Exp. Med. 198, 1323–1335 (2003).
Article PubMed PubMed Central CAS Google Scholar
Wang, J., Marschner, S. & Finkel, T. CXCR4 engagement is required for HIV-1-induced L-selectin shedding. Blood 103, 1218–1221 (2004).
Article PubMed CAS Google Scholar
Klatt, N. R. et al. Limited HIV infection of central memory and stem cell memory CD4+ T cells is associated with lack of progression in viremic individuals. PLoS Pathog. 10, e1004345 (2014).
Article PubMed PubMed Central CAS Google Scholar
Paiardini, M. et al. Low levels of SIV infection in sooty mangabey central memory CD(4)(+) T cells are associated with limited CCR5 expression. Nat. Med. 17, 830–836 (2011).
Article PubMed PubMed Central CAS Google Scholar
Munoz-Calleja, C. et al. Highly active antiretroviral therapy induces specific changes in effector and central memory T cell sub-populations. AIDS 15, 1887–1890 (2001).
Article PubMed CAS Google Scholar
French, M., Keane, N., McKinnon, E., Phung, S. & Price, P. Susceptibility to opportunistic infections in HIV-infected patients with increased CD4 T-cell counts on antiretroviral therapy may be predicted by markers of dysfunctional effector memory CD4 T cells and B cells. HIV Med. 8, 148–155 (2007).
Article PubMed CAS Google Scholar
Younes, S. A. et al. HIV-1 viremia prevents the establishment of interleukin 2-producing HIV-specific memory CD4+ T cells endowed with proliferative capacity. J. Exp. Med. 198, 1909–1922 (2003).
Article PubMed PubMed Central CAS Google Scholar
Sallusto, F., Lenig, D., Förster, R., Lipp, M. & Lanzavecchia, A. Two subsets of memory T lymphocytes with distinct homing potentials and effector functions. Nature 401, 708–712 (1999).
Article ADS PubMed CAS Google Scholar
Le Gall, S. M. et al. ADAMs 10 and 17 represent differentially regulated components of a general shedding machinery for membrane proteins such as transforming growth factor alpha, L-selectin, and tumor necrosis factor alpha. Mol. Biol. Cell 20, 1785–1794 (2009).
Article PubMed PubMed Central Google Scholar
Wang, R. & Sun, P. D. Natural killer cell-mediated shedding of ULBP2. PLoS ONE 9, e91133 (2014).
Article ADS PubMed PubMed Central CAS Google Scholar
Peschon, J. J. et al. An essential role for ectodomain shedding in mammalian development. Science 282, 1281–1284 (1998).
Article ADS PubMed CAS Google Scholar
Mohler, K. M. et al. Protection against a lethal dose of endotoxin by an inhibitor of tumour necrosis factor processing. Nature 370, 218–220 (1994).
Article ADS PubMed CAS Google Scholar
Kahn, J., Ingraham, R. H., Shirley, F., Migaki, G. I. & Kishimoto, T. K. Membrane proximal cleavage of L-selectin: identification of the cleavage site and a 6-kD transmembrane peptide fragment of L-selectin. J. Cell Biol. 125, 461–470 (1994).
Article PubMed CAS Google Scholar
Gentile, M. et al. Determination of the size of HIV using adenovirus type 2 as an internal length marker. J. Virol. Methods 48, 43–52 (1994).
Article PubMed CAS Google Scholar
Pearce-Pratt, R., Malamud, D. & Phillips, D. M. Role of the cytoskeleton in cell-to-cell transmission of human immunodeficiency virus. J. Virol. 68, 2898–2905 (1994).
PubMed PubMed Central CAS Google Scholar
Jolly, C., Kashefi, K., Hollinshead, M. & Sattentau, Q. J. HIV-1 cell to cell transfer across an Env-induced, actin-dependent synapse. J. Exp. Med. 199, 283–293 (2004).
Article PubMed PubMed Central CAS Google Scholar
Mehta-D’souza, P. et al. Glycan bound to the selectin low affinity state engages Glu-88 to stabilize the high affinity state under force. J. Biol. Chem. 292, 2510–2518 (2017).
Article PubMed CAS Google Scholar
Vassena, L. et al. HIV-1 Nef and Vpu interfere with L-Selectin (CD62L) cell surface expression to inhibit adhesion and signaling in infected CD4+ T lymphocytes. J. Virol. 89, 5687–5700 (2015).
Article PubMed PubMed Central CAS Google Scholar
Eisele, E. & Siliciano, R. F. Redefining the viral reservoirs that prevent HIV-1 eradication. Immunity 37, 377–388 (2012).
Article PubMed PubMed Central CAS Google Scholar
Chun, T. et al. Presence of an inducible HIV-1 latent reservoir during highly active antiretroviral therapy. Proc. Natl. Acad. Sci. USA 94, 13193–13197 (1997).
Article ADS PubMed CAS Google Scholar
Chun, T. et al. Quantification of latent tissue reservoirs and total body viral load in HIV-1 infection. Nature 387, 183–188 (1997).
Article ADS PubMed CAS Google Scholar
Finzi, D. et al. Identification of a reservoir for HIV-1 in patients on highly active antiretroviral therapy. Science 278, 1295–1300 (1997).
Article ADS PubMed CAS Google Scholar
Wong, J. K. et al. Recovery of replication-competent HIV despite prolonged suppression of plasma viremia. Science 278, 1291–1295 (1997).
Article ADS PubMed CAS Google Scholar
Sgarbanti, M. & Battistini, A. Therapeutics for HIV-1 reactivation from latency. Curr. Opin. Virol. 3, 394–401 (2013).
Article PubMed CAS Google Scholar
Zou, Z. & Sun, P. D. Overexpression of human transforming growth factor-beta1 using a recombinant CHO cell expression system. Protein Expr. Purif. 37, 265–272 (2004).
Article PubMed CAS Google Scholar
He, J. et al. Human immunodeficiency virus type 1 viral protein R (Vpr) arrests cells in the G2 phase of the cell cycle by inhibiting p34cdc2 activity. J. Virol. 69, 6705–6711 (1995).
PubMed PubMed Central CAS Google Scholar
Cho, M. W. et al. Identification of determinants on a dualtropic human immunodeficiency virus type 1 envelope glycoprotein that confer usage of CXCR4. J. Virol. 72, 2509–2515 (1998).
PubMed PubMed Central CAS Google Scholar
Moir, S. et al. B cells of HIV-1-infected patients bind virions through CD21-complement interactions and transmit infectious virus to activated T cells. J. Exp. Med. 192, 637–646 (2000).
Article PubMed PubMed Central CAS Google Scholar
Chun, T.-W. et al. Effect of antiretroviral therapy on HIV reservoirs in elite controllers. J. Infect. Dis. 208, 1443–1447 (2013).
Article PubMed PubMed Central CAS Google Scholar
Zhu, P. et al. Distribution and three-dimensional structure of AIDS virus envelope spikes. Nature 441, 847–852 (2006).
Article ADS PubMed CAS Google Scholar
Johannes, S. et al. Fiji: an open-source platform for biological-image analysis. Nat. Methods 9, 676–682 (2012).
Article CAS Google Scholar

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Acknowledgements

We thank Dr. Gary Gilliland of Janssen for kindly providing the OKT3 antibody. We thank Mr. Nathan Max for his help in producing recombinant gp120. This work was supported by the Intramural AIDS Targeted Antiviral Program (IATAP) fund and Intramural Research of National Institute of Allergy and Infectious Diseases, National Institutes of Health.

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These authors contributed equally: Joseph Kononchik, Joanna Ireland.

Authors and Affiliations

Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 12441 Parklawn Drive, Rockville, MD, 20852, USA
Joseph Kononchik, Joanna Ireland, Zhongcheng Zou, Jason Segura, Genevieve Holzapfel, Ashley Chastain, Ruipeng Wang, Matthew Spencer, Biao He, Nicole Stutzman, Daiji Kano & Peter Sun
Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD, 20892, USA
James Arthos, Tae-Wook Chun & Susan Moir
Research Technology Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 South 4th Street, Hamilton, MT, 59840, USA
Elizabeth Fischer

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Joseph Kononchik
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Joanna Ireland
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Zhongcheng Zou
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Ruipeng Wang
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Matthew Spencer
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Biao He
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Daiji Kano
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Elizabeth Fischer
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Tae-Wook Chun
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Susan Moir
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Peter Sun
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Contributions

J.K., J.I., Z.Z., J.S., E.F., T.-W. C., and P.S. designed the experiments. J.K., J.I., Z.Z., J.S., G.H., A.C., R.W., M.S., B.H., N.S., D.K., E.F. and T.-W. C. carried out the experiments and analyzed the results. J.K., A.C., T.-W.C., and P.S. wrote the paper. J.A. and S.M. provided reagents and technical guidance.

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Correspondence to Peter Sun.

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Kononchik, J., Ireland, J., Zou, Z. et al. HIV-1 targets L-selectin for adhesion and induces its shedding for viral release. Nat Commun 9, 2825 (2018). https://doi.org/10.1038/s41467-018-05197-2

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Received: 19 October 2017
Accepted: 23 May 2018
Published: 19 July 2018
DOI: https://doi.org/10.1038/s41467-018-05197-2

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