Figure 9: Nature Communications

Fig. 9

From: Structural basis of adaptor-mediated protein degradation by the tail-specific PDZ-protease Prc

Fig. 9

Role of the hinge residues of Prc in NlpI-mediated substrate degradation. ae SDS-PAGE assays monitoring sNlpI-mediated sMepS degradation by Prc-K308W a, the catalytically inactive Prc-K477A as a reference b, Prc-L245A c, Prc-L340A c, Prc-L340G d, and Prc-L340G/L245A e. f A lever-like working model for degradation of protein substrate containing a flexible C-terminal tail by the Prc-NlpI complex. The initial cycle of substrate-docking (top) and ligand-triggered PDZ movement (bottom) steps are shown. See Discussion for details. The substrate C terminus is indicated by an asterisk. The ligand-sensing hydrophobic residues at the hinge are represented by a bifurcated side chain colored in red. The lipid anchors are depicted by colored curved lines linking to the outer membrane (OM). Sites for intramolecular interaction (e.g., the NHD and helix h9) and ligand binding in Prc are depicted by short black curves